자주초롱꽃(Campanula punctata 'Rubriflora')의 자가영양배양시 CO2 농도,환기횟수 및 광도가 소식물체의 생장에 미치는 영향을 조사하였다. 1,500μmol·m-1고농도CO2에서는 광도를 높일수록 초장, 엽수가 증가되었으나, 최대근장은 유의성이 인정되지 않았다 엽록소의 함량은 광도가 높을 때보다는 낮은 경우인 70μmol·m-2s-1에서 가장 많이 증가되었다 엽면적은 광도가 높을수록 작아지는 경향을 보였으며, CO2 1,500μmol·mol-1 환기횟수 2.8h-l, PPF 220μmol·m-2·s-1에서 가장 큰 차이로 작게 나타났다. 생체중과 건물중은 CO2 농도, 환기횟수, PPF가 높을수록 증가되었다. 특히 CO2 1,500μmol·mol-1, 환기횟수 2.8h-1, PPF 220μmol·m-2·s-1에서 가장 크게 증가되었다. 환기횟수의 영향은 전반적으로 적었다. 종합적으로 자주초롱꽃 소식물체의 기내 자가영양배양 차량번식을 위해서는 CO2 1,500μmol·mol-1, PPF 220μmol·m-2·s-1의 조건이 적합하였다.

Background : The causative organism of Astragalus membranaceus (Hwanggi) root rot disease was known as Pythophthora drechsleri. In this study, a new species of root rot causative organism has been identified and reported. Methods and Results : Hwanggi, which has root rot disease, was collected in Jungsun-gun, Gangwon-do. The diseased root was cut into 1 ㎝ size and disinfected with 1% sodium hypochlorite solution for 1 minute and washed with sterile distilled water. The dried samples were dipped in the PDA medium and cultured in at 25℃ incubator. The 21 isolates were cultured under the same conditions and genomic DNA was extracted. The genomic DNA of isolated strains was extracted using a kit of Biofact. The isolated strains were identified using internal transcribed spacer (ITS) region sequencing. The ITS region was amplied using the primer pair ITS1 (5’-TCCGTAGGTGAACCTGCGG-3’) and ITS4 (5’-TCCTCCGCTTATTGATATGC-3’). The PCR was performed in a final volume of 50 ㎕ containing 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 1.5 mM MgCl2, each dNTP at a concentration of 0.2 mM, 1.25 IU of Taq polymerase, each primer at a concentration of 10 pmol, and 2ul of the DNA template. Sequencing was performed by asking Macrogen. The analyzed sequences were compared using the Blast program provided by NCBI. As a result of identification of 21 strains, 13 Fusarium spp., 1 Peronospora sp. (causative organism of downy mildew), 3 Acremonium spp., 3 Uncultured fungus, 1 Phyrtophthora sp.. The main pathogen of wilt disease was known as Fusarium sp., a soil-borne pathogen. After analyzing the systematic taxonomic relationships, the systematic diagram was created using the neighbor-joining method. Conclusion : We have finally identified Phytophthora cryptogea as a causative organism of Hwanggi root rot disease and will check whether the disease has occurred on the inoculation through pot test.

Background : Atractylodes macrocephala is a perennial herbaceous plant belonging to the family Asteraceae and should be cultivated in field soils with good water dripping due to plant characteristics. However, cultivating farmers mainly have recently been cultivated in paddy soil due to their regional characteristics, which causes the decrease in yield due to poor drainage. Therefore, this study was carried out to investigate the cultivation in high ridge and subsoil breaking effect for stable paddy soils cultivation technology of A. macrocephala. Methods and Results : Soil was paddy soils in the fall of 2017, and the pH (1 : 5) was 6.61 ± 0.15 as a result of chemical and physical properties. EC was 0.49 ± 0.05 dS/m, and organic matter content was 28.69 ± 69 g/㎏ and effective phosphoric acid was 306 ± 17.8 ㎎/㎏. As a result of the soil layering survey, the surface layer was 0 - 26 ㎝ deep as paddy soil mounded with sandy loam in the past. In the surface layer, there was a light layer after 17 ㎝ depth, and volume density was 1.71 ± 1.3 g/㎤. The porosity of the plow pan was 33.41 ± 2.34%. The cultivation methods were cultivation in high ridge (30 ㎝ or more) and level row (10 ㎝ or less) at 2 levels and 3 repetitions. In subsoil breaking, the depth of the plow pan was increased from 17.1 ± 0.5 ㎝ before treatment to 31.1 ± 3.6 ㎝ after treatment and the hardness was 24.8 ± 1.5 ㎜. In the case of rotary plowing, the depth of the plow pan was 17.1 ± 1.9 ㎝ before treatment and 26 ± 2.4 ㎝ after treatment and the hardness was 25.8 ± 2.9 ㎜. The medium growth characteristics of A. macrocephala per treatment showed the tendency of increase in plant length, culm length, number of nodes, number of leaves, and fresh weight in level row cultivation after subsoil breaking. Root growth of cultivation in high ridge after subsoil breaking tended to be good with rhizome weight of 11.6 g per hill. The survival percentages were 98.8 - 100% and the bolting rate was 93.4 - 96.2% Conclusion : In cultivation in high ridge after subsoil breaking in the paddy field of Gangwon area, the decrease of yield of A. macrocephala due to drainage was expected to be alleviated, but final conclusion should be drawn after analyzing soil temperature and soil moisture data.