To estimate the genetic characteristics and cumulative power of discrimination (CPD) existing among Hanwoo (Korean cattle) and exotic foreign population (Angus, Herford, Charolais, Holstein) we used a total of 414 genomic DNAs from five breeds population (Hanwoo, Angus, Hereford, Charolais, Holstein). Genetic characteristics indices including mean allele number among loci, unbiased heterozygosity () within locus and polymorphic information content (PIC) and unbiased average heterozygosity (H) among loci in four breeds were calculated using the generated allele frequencies by each marker. The mean allele numbers for all loci ranged between 5 and 7 while heterozygosity (H) ranged from 0.75 (HW) to 0.64 (HF) among loci and across breeds heterozygosity (H) was 0.69. The generated unbiased average heterozygosity among loci in each breed was integrated to the global formula of CPD resulting in 99.71 % within the populations. The genetic variation of HW (Hanwoo) showed highest estimates among the analyzed breeds.

본 연구는 후보 유전자의 경제형질에 미치는 염기 변이 효과를 검증하기 위해 국내에서 사육된 듀록 품종 96두와 한국 재래 돼지 86두를 활용하였다. 검증에 활용된 4개의 후보 유전자는 MC4R, PA-KAG3, FABP3 그리고 ESR 유전자였다. 각 후보 유전자들의 유전자형 분석 결과 두 집단 간에 유전적 특성의 차이가 분명히 나타나고 있음을 확인하였다. MC4R 유전자의 A 대립 유전자는 두 집단 모두에서 성장 형질과의 유의한 연관성이 검출되었고,

본 연구는 젖소(Holstein종)의 mtDNA D-loop 영역 염기변이 다형성과 경제형질간의 관련성을 분석하기 위하여 수행하였다. 젖소의 mtDNA D-loop 영역에서 단일염기의 치환에 의해 총 35개의 polymorphic site가 확인되었다. 그중 주요 Polymorphic site의 염기변이 빈도는 106, 169, 16057, 16231 및 16255 지역에서 높은 빈도의 염기치환이 검출되었다. 169 지역은 A가 G로 염기치환이 일어났고 그 빈도는 0.555로 높게 나타났으며 염기치환에 의한 산유량 효과는 1259.6 kg(P<0.05)로 나타났다. 유지방과의 관계를 분석한 결과 16118 지역은 A가 G로 치환되는 빈도가 0.02로 검출되었으며, 16135 지역은 T가 C로 치환되는 빈도가 0.02로 검출되었고, 16302 지역은 G가 A로 치환되는 빈도가 0.04로 검출되었다. 이 지역들이 유지방에 미치는 변이 효과는 - 156, - 118.15 및 - 67.77 kg(P<0.1)으로 나타났다. 본 연구에서 검출한 젖소 mtDNA내 D-loop 영역의 염기서열 변이 빈도와 유량, 유지방과의 연관성 분석 결과 등은 젖소집단의 유전적 변이성 추정과 좀 더 다양한 경제형질과의 관련성 분석으로 다양한 유전적 지표인자 발굴에 도움이 될 것이며 이를 통해 분자유전학적 기법을 이용한 젖소의 육종전략을 확립하는데 기초 자료가 되는 것은 물론 분자육종학적인 연구에 기초 자료로서 유용하게 활용할 수 있을 것으로 기대된다.

요크셔종과 버크셔종 교배 실험 집단을 활용하여 양적형질 유전자좌 (QTL)의 발현 특성 관련 유전 양식을 조사하였다. 총 512두의 F 자손이 F간의 65교배 조합으로부터 생산되었으며 표현형 조사 기록은 일당증제량(ADG), 평균 등지방 두께(ABF), 10번째 등뼈 부위 등지방 두께(TRF) 및 등심단면적(LEA), 최후 척추부위 등지방 두께 (LRF)였다. 125종의 유전자 표지 (microsatellite)에 대한 3세대 개체별 유전자형이 분석되었

This study was carried out to establish an effective system for embryo transfer techniques by analyzing several factors affecting in-vivo embryo transfer in Korean cattle. Embryos produced in-vivo were transferred into a total of 301 recipients The results obtained in studies on the factors affacting pregnancy rate after embryo transfer by condition of recipients were as follows ; 1. The pregnancy rate by age and parity of recipients showed high in 5~8 and over 12 years old(72.7~73.9%), and 3rd~4th parity(82.1%) for fresh embryos(P<0.05). The pregnancy rate did not differ by age and parity of recipients in frozen embryos. The pregnancy rate of frozen embryos tended to be similar to that of fresh embryos(38.5% and 25.0~36.7%). 2. The number of observation for normal estrus cycles of recipients did not differ In pregnancy rate between one and 2 times in fresh embryos(64.9%, 69.8%). The pregnancy rate by transferred frozen embryos showed significantly higher after 2 times of observation(P<0.05, 16.3%, 37.5%). The pregnancy rate by days open did not differ between fresh and frozen embryos. But the pregnancy rate was slightly higher in 12 months and 6 months of days open for fresh and frozen embryos, respectively(70.1~71.1% and 24.5%, respectively). 3. The pregnancy rate of transferred fresh and frozen embryos into right and left side of uterine horn did not differ(62.1% : 65.9% 25.0% : 24.3%, respectively). The pregnancy rate by the grade of CL was not different in fresh embryos, but the pregnancy rate was significantly higher in the grade A than B for frozen embryos(P<0.01, 43.2%, 16.2%).

This study was carried out to establish an effective and practical system for commercialization of embryo production techniques by analyzing several factors influencing in vivo embryo production on condition of donors treated FSH-P and SUPER-OV in Korean native cattle. In vivo embryos were flushed 226 times from 128 donors. The results obtained for the factors influencing in vivo embryo production by conditionof donors treated FSH-P and SUPER-OV were as follows :1. Age and parity of donors did not affect the transferable and freezable embryos among the treatments in FSH-P but the transferable and freezable embryos were decreased after 6 years old and 4th parity in SUPER-OV(P<0.01). 2. The production of embryos on the weight of donors was higher in over 400kg of body weight in FSH-P(P<0.01) and was higher in over 450kg than 400~450kg of body weight in SUPER-OV(P<0.05). For FSH-P embryo production was better responded in 350~450kg of body weight with 30~32mg doses, and showed a better result in over 450kg body weight with 32~34mg doses.(Key Words : in vivo embryo, donors, FSH-P SUPER-OV)

본 연구는 수정능획득유기물질로 알려진 카페인 헤파린을 병행처리하여 한우 정자의 첨체 반응율과 생존율을 알아보고 수정능획득과정 중에 단백질의 변화상을 전기영동방법으로 조사하였다. 동결융해후 정자의 생존율은 90%이상이었으나 전배양처리후 0.5시간에 70%로 감소하고 2시간 이후에는 35%로 감소하였다. 정자의 첨체반응율은 동결융해후 정상정자가 85.7%였으나 전배양시간에 따라 53.4%에서 14.3%로 감소하였다. 동결융해후 첨체가 소실된 생존정자는 9.

다배란 수정란이식(MOET)과 체외수정(IVF)기술을 이용한 육종체계에서 예상되는 유전적 개량량을 여러 집단의 크기에서 비교한 결과 체외수정기술이 MOET육종기술보다 특히 개량대상 유전력이 낮을때 훨씬 효율적으로 나타났다. 그러나 유전력이 높아지면 (h=0.3, 0.5), MOET와 IVF간의 상대적 차이는 미진하게 나타났다. 체외수정을 이용한 육종기술에서 암컷의 선발 강도는 대단히 높일 수 있는 반면 난자의 회수율이 많을수록 상대적으로 수컷에 대한 선

Genetic changes from improving female's reproductive rate through in vitro fertilization of large number of oocytes were studied. The breeding scheme employed was multiple ovulation and embryo transfer of juveniles and adults. Both balanced and unbalanced matings were examined for the four closed progeny population sizes, 10, 10, 105, 106. In balanced matings, all selected sires and dams were mated to each other(cross-classified mating) while unbalanced matings allowed selected dams and sires mated partially, eg. unbalanced matings allowed averages of .5 and .25 progeny per each mating. Various numbers of selected sires and dams were also examined in both balanced and unbalanced matings. In all mating schemes, selection of males and females was restricted to he one from each fullsib family to reduce the rate of inbreeding. The model calculations were deterministic and accounted for the effects of selection and inbreeding on loss of the genetic variation in succeeding generations. Balanced rectangular mating schemes, where more donors were selected than sires, resulted in larger selection responses than balanced square mating schemes, where equal numbers of sires and donors were selected, and unbalanced rectangular mating. The first round selection responses from the balanced rectangular matings of juvenile MOET, eg. number of progeny per mating equals 2 with 10 sires selected, were 1.192, 1.406, 1.580 and 1.735 times larger than the first round selection responses from the balanced square mating schemes for the given four progeny population sizes, 10, 10, 105 and 106, respectively. Similar results were obtained in adult MOET breeding schemes. However, balanced square matings gave greater selection responses than the unbalanced rectangular matings.

본 연구는 한우의 지역별 유전적 다양성 및 집단간의 유연관계를 평가하기 위하여 경기, 강원, 충남, 충북, 전남, 전북, 경남 그리고 경북 8개 지역에서 비교적 지역간의 이동이 적은 번식우를 대상으로 공시축을 선발하여 분석을 실시하였다. 각 microsatellite marker의 평균 대립유전자의 수는 7.5개로 검출되었다. 관측된 대립유전자의 이형접합율을 보면 전북(CEB)과 전남(CEN)에서 가장 높게 나타났지만 기대되는 이형접합율은 전체 평균보다

In an animals, identification system has been widely used by ear tag with dummy code and blood typing for parernity. Also, genotyping methods were using for useful mean of individual identification for live animals. In the case of genotyping estimation of gene in population of korean native chicken. In this study, we tested for development of genetic markers used it possible to determination of individual identification system. The candidate genetic markers were used already know 10 of microstalite DNA sequence information in chromosome No. 1 and 14. Result of analysis for genotyping, the number of alleles of those microstatelites DNA was shown minimal 3 to 12 and the heterozygote expression frequency range was shown from 0.617 to 0.862. In our result, effective number of allele for each microsatellites DNA was shown 3~7, and the accuracy of individual identification was shown nearly 100%, when used with 6 genetic marker. This study was about genotyping method for identification used specific genetic marker form microsatellite DNA in the brand marketing of korean native chicken. Our results suggest that genotyping method used specific genetic marker from microsatellite DNA might be very useful for determination of individual identification.

Identification of animals has been used with an ear tag with dummy code and blood typing has been used for paternity and individual identification in live animals. Various genetic markers are different for breeds of pig and hence, it is necessary to identity the discrete genetic marker in korean native pig. A total of 240 pigs were used to find korean native pig population specific markers that expressed in population of korean native pigs. To identify the individual traceability, 20 animals were randomly chosen and tested for a whole process from being live to slaughter stages. The candidate genetic marker used in the study were 18 DNA microsatellites which were identified in pig genome. The number of alleles of those DNA microsatellites ranged form a minimum of 3 to maximum of 6. The heterozygote frequency ranged from 0.44 to 0.69. Effective number of alleles for each DNA microsatellotes were 2 to 4. By choosing 6 candidate genetic markers among all, the traceability of individual identification was estimated as accurate as 99.99%(p＞0.0014), nearly.