본 실험은 국화의 바이로이드 제거에 이용되는 초저온처리 시 국화 품종 'White ND'을 적합한 처리조건을 확립하기 위해 초저온처리의 단계별 요인을 실험하였다. 그 결과 생장점의 크기는 1 mm(엽원기 2~3매 포함)에서 높은 생존율과 신초 재생율을 나타내었고, vitirification 처리시 PVS3가 효과적이었으며, 처리 시간은 60분 처리 하였을 때 높은 생존율 및 정 상 신초 재생율을 보였다. 또한 vitrification을 위한 전처리 조건은 sucrose 농도를 88 mM 24시간, sucrose 0.3 M 16시간, sucrose 0.5 M 6시간, sucrose 0.7 M 3시간으로 처리하는 것이 초저온 처리 후 생존율 및 신초 재생율을 높이는데 효과적이었으며, 재생된 정상 식물체는 모본과 비교하여 ploidy level이 동일한 것으로 보아 식물체의 유전적 변이가 일어나지 않았다.

화경을 상, 중, 하부로 나누어 신초 및 자구를 유도하기 위하여 NAA와 몇 종류의 cytokinin 첨가 배지에서 각 부위별 화경 절편체를 배양해서 얻은 결과는 다음과 같다. 화경 조직에서 상부, 중부, 하부 중 소화경이 부착된 상부조직에서 다아체 및 자구 형성이 전반적으로 가장 양호 하였다. 다아체 형성은 NAA 와 TDZ 혼용배지에서 가장 효율적이었고 자구 형성은 NAA 와 kinetin 또는 NAA 와 kinetin 혼용배지에서 가장 효율적이었다. 온실에서 순화된 식물체는 정상적으로 개화하였으며 재식 2년차에 1.5개 내외의 자구를 가진 구를 생산할 수 있었다.

본 연구는 우리나라 주요 재배종인 Muscari armeniacum ‘Early Giant’ 품종을 사용하여 엽절편체로 부터 직접적으로 신초재생과 체세포배 발생에 미치는 생장조절제의 효과를 구명하였다. 무스카리의 엽조직으 로부터 캘러스 과정을 거치지 않은 직접 신초형성은 2,4-D 0.1 mg·L−1가 함유된 배지에서 가장 좋았다. 반면, 체세포배 발생은 생장조절제를 첨가하지 않는 대조구와 IPA 0.1~1.0 mg·L−1가 함유된 농도의 배지에서 비교적 양호하였다. 무스카리의 엽조직으로부터 재생된 자구를 기외로 이식했을 때 맹아율은 모든 처리구에서 80%이상 으로 높았으며 특히 NAA 0.1mg·L−1, IPA 1.0~3.0mg·L−1 배지에서 재생된 자구의 생장이 양호하였다.

ZnO nanostructures were grown on an Au seed layer by a hydrothermal method. The Au seed layer was deposited by ion sputter on a Si (100) substrate, and then the ZnO nanostructures were grown with different precursor concentrations ranging from 0.01 M to 0.3M at 150˚C and different growth temperatures ranging from 100˚C to 250˚C with 0.3 M of precursor concentration. FE-SEM (field-emission scanning electron microscopy), XRD (X-ray diffraction), and PL (photoluminescence) were carried out to investigate the structural and optical properties of the ZnO nanostructures. The different morphologies are shown with different growth conditions by FE-SEM images. The density of the ZnO nanostructures changed significantly as the growth conditions changed. The density increased as the precursor concentration increased. The ZnO nanostructures are barely grown at 100˚C and the ZnO nanostructure grown at 150˚C has the highest density. The XRD pattern shows the ZnO (100), ZnO (002), ZnO (101) peaks, which indicated the ZnO structure has a wurtzite structure. The higher intensity and lower FWHM (full width at half maximum) of the ZnO peaks were observed at a growth temperature of 150˚C, which indicated higher crystal quality. A near band edge emission (NBE) and a deep level emission (DLE) were observed at the PL spectra and the intensity of the DLE increased as the density of the ZnO nanostructures increased.

캘러스, 반수체, picloram, 전처리양식을 규명할 목적으로 배양효율에 미치는 화분발육 단계와 온도전처리가 미치는 영향에 대한 실험결과는 다음과 같다. ‘Dreamland’의 감수분열중인 약을 현 미경적방법으로 검경하여 본 결과 화뢰의 길이가 23.0-24.9mm는 tetrad 단계이었고 25.0-26.9mm는 uninucleate 단계, 27.0-28.9mm에서는 binucleate 단계 임을 확인할 수 있었으며, 저온 및 고온 온도처리 결 과는 전체적으로 캘러스 및 배 발생율은 저조하였고 특 이한 점은 발견할 수 없었다. Microgametogenesis 단 계별로 약배양 효율을 조사한 결과, late uninucleateearly binucleate 단계인 23.0-28.0mm에서 17.8% callus가 유도되었고, 식물체 재분화율도 6.7%로 나타 나 적정 배양 stage임을 확인할 수 있었다. 유기된 캘 루스를 picloram과 zeatin을 혼용한 MS 배지에 이식 하여 25oC에서 배양하여 식물체를 얻었다.

The purpose of this research is describing the structural behavior of the Head-Splice Sleeve by a finite element analysis. It is consistent with the experimental date that considering the contact element between mortar and structural steel. This paper is trying to propose that the results of analysis method can be used on the future Finite Element Analysis．

In this study, compressive capacity of Form-Block-Wall (FBW) was studied through prism test. Main variables are the difference in strength of grout concrete and steel fiber-reinforced block. The result showed that the strength of FBW increased corresponding to the increase of the strength of grout concrete and mortar. Also, it showed that steel fiber-reinforced FBW had a positive effect on prism strength and deformation capacity.

In this paper, the seismic performance of RC frame with deterioration of structural capacity was evaluated by using capacity spectrum method. And the efficiency of retrofit by using infilled masonry was checked from the evaluation of its seismic capacity for various layout. As a results, it was found that the masonry infill wall increased the stiffness and strength, but reduced ductility.

In this study, compressive behavior of form-block-wall was studied through prism test. In the experiment, main variables are the difference in mix design of block and strength of grout concrete. Experiment result showed that the prism strength of form-block-wall increased in proportion to the strength of grout and block. The initial stiffness in load-displacement curve varied corresponding to the difference of block strength in low strength of grout concrete while there was not a difference of it in the case of high strength of grout concrete.

This paper presents a finite element analysis to evaluate thermal conductivity of FRP (Fiber Reinforced Polymer) retrofit system in reinforced concrete structure. Especially, this paper focused on near-surface-mounted retrofit method; FRP is mounted into the groove after making a groove in concrete. Midias-FEA program was used to analyze the case that the epoxy was covered by various board to resist fire such as, calcium silicate or alumino silicate board. Analysis results give that the prediction of temperature transition from surface is possible by FE analysis and the result can be used to evaluate fire resistance of certain fire proof materials in FRP retrofit system.

In this study, compressive capacity of a new form-block-wall (FBW) was studied through both the prism test and compressive strength test for single form-block. In the experiment, main variables are the difference in mix design of block and in strength of grout concrete. The result of prism test showed that the compressive strength of FBW was largely influenced by the strength of grout concrete while it was not influenced by the block‘s mix design difference.

Recently, retrofit by using fiber reinforced polymer (FRP) has been widely applied to building structure. However, it loose the retrofit contribution under high temperature due to fire because of its low glass transition temperature. In this manner, this paper presents a study on the development of fire resistant capacity in FRP retrofit system. Thermal transition of various fire resistant materials were verified by performing high temperature loading test. As a result, it was found that calcium silicate and alumino silicate had high resistance on the heat transition.

This paper presents an analytical approach to evaluate a possibility of progressive collapse of reinforced concrete frame structure. In order to simulate adequate behavior of the structure, we considered the bond failure of lap spliced bars due to concrete crack in a plastic hinge region. Analysis result gave that there was additional deflection due to the bond failure of bar in beams at the node where the increasing displacement load was applied. The stress was concentrated to the beam where a column was removed, and as it goes up to higher stories, the moment change of an upper story appeared less.

Recently, retrofits by using fiber reinforced polymer (FRP) have been widely performed for strengthening of reinforced concrete structure. However, in spite of the weakness of FRP for fire, the research result of it is not enough for practical use. In korea, fire is most frequent disaster which occurs in building. This means the improved structural capacity of building by a retrofit with FRP may be lost by fire if the FRP was not properly protected from it. In this paper, a fire analysis result is presented for the evaluation of damage of reinforced concrete member enhanced by FRP. As a result, the loss of strength of RC member is able to be predicted by using FE analysis with proper material properties for temperature variation.

Recently, retrofits by using fiber reinforced polymer (FRP) have been widely performed for strengthening of reinforced concrete structure. However, in spite of the weakness of FRP for fire, the research result of it is not enough for practical use. In korea, fire is most frequent disaster which occurs in building. This means the improved structural capacity of building by a retrofit with FRP may be lost by fire if the FRP was not properly protected from it. This paper present a fire test of concrete block reinforced by FRP. From the test, it was found that the bond capacity of FRP attached to concrete decreased from temperature loading of 65℃.