Background : Haskap berries commonly refer to fruits of Lonicera caerulea L., recognized by the Japanese aborigines as the “The elixir of life.”. Due to their recent arrival on the North American market, haskap berries have not yet been positioned among other berries and compared in terms of their phytochemical content. And haskap berries have higher ascorbic acid and anthocyanin content than other berries known for their health-promoting benefits, such as blueberries. However, no study has reported on the antioxidant and anti-cancer activity of Lonicera caerulea stem. The purpose of this study is to present the current research on the chemical content, antioxidant and anti-cancer activities of Lonicera caerulea stem. Methods and Results : The stem of Lonicera caerulea L. ware dried in the shade at room temperature and extracted with 100% methanol. The extract was suspended in deionized water and partitioned sequentially with n-hexane, chloroform, ethyl-acetate and butanol (water saturated BuOH) fractions. Antioxidant activities were measured by determination of antioxidants, DPPH (2,2-diphenyl-1-picrylhydrazyl). Cell viability was determined by the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay. All cell lines were purchased from the Korean Cell Line Bank (Seoul, Korea). All results were performed with three replications were processed statistically. By DPPH assay, the Lonicera caerulea L. the highest activity was obtained from the ethyl-acetate fraction (IC50=15.46 ㎍/㎖). By MTT assay, the chloroform fraction showed a significant growth inhibiting effect on MCF-7 (Human breast cancer, IC50=225.91 ㎍/㎖), COLO 205 (Human colon cancer, IC50=179.55 ㎍/㎖), but on AGS (Human stomach cancer) and other fractions it did not show effect. Conclusion : We demonstrated that Lonicera caerulea L. stem extract and fractions has antioxidant and antiproliferation activity in vitro. Further studies should identify the active constituents in Lonicera caerulea L stem to evaluate the potential in vitro antioxidant and antiproliferation activities of the extract.

Background : Perilla frutescens L. is valuable as a medicinal plant as well as a natural medicine and functional food. Limonene perilla collected from various places showed 60% limonene compounds. However biological activity of these accession has not been reported before. Therefore, this study was conducted to investigate the biological activity of limonene perilla. Methods and Results : Fractional solvent extracts were obtained by using organic solvents such as n-hexane, chloroform, ethyl acetate, n-BuOH, and aqueous solvent from different parts of limonene perilla extracted initially in 70% EtOH. We investigated the effects of limonene perilla on total phenol and flavonoid contents, FRAP (Ferric Reducing Antioxidant Power), total saponin contents and tyrosinase inhibition activity. Leaves of limonene perilla produced the highest total phenolic contents (29.88 mg·CAE/g), flavonoid (8.39 mg·QE/g) and saponin contents (47.77 mg·GIE/g) than stems and roots of limonene perilla. FRAP of leaves was 823.00±3.58 μM·FeSO4·E/mg. Tyrosinase inhibition activity rate was 40.31% in 70% ethanol extracts from leaves of limonene perilla. Conclusion : This results suggest that leaf of limonene perilla fractions has significant antioxidant activity. Also, limonene perilla could be used as a functional biomaterial in developing cosmetics and functional foods.

Background : This study aimed to investigate the antioxidant and anti-inflammatory activities of water chestnut (Trapa japonica Flerow) extract. Methods and Results : The antioxidant and anti-inflammatory activities of 100% methanol extract of water chestnut were investigated. The methanol extract was evaluated for its total phenolic and flavonoid content, DPPH•(1,1-diphenyl-2-picrylhydrazyl) free-radical scavenging activity,reducing power, andeffect on nitric oxide (NO) production and cell viability using real-time polymerase chain reaction (qPCR). The total phenolic content was 438.31 ㎍ allic acid equivalent (GAE)/㎎ extract and the total flavonoid content was 61.40 ㎍ quercetin equivalent (QE)/㎎ extract. In addition, results revealed the extract possessed antioxidant activity (DPPH• free-radical scavenging activity) with IC50 value of 5.28 ㎍㎖ The reducing power of the extract was assayed spectro photometrically and showed Abs of 0.71 at 100 ㎍㎖ Furthermore, extracts of water chestnut exhibited no cytotoxicity in RAW 264.7 cells. In addition, the NO assay revealed that LPS-induced NO production was significantly inhibited following treatment with water chestnut extracts. The expression of pro-inflammatory proteins such as inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 decreased in a concentration-dependent manner. The water chestnut extract also decreased tumor necrosis factor α (TNF-α) release. Conclusion : Therefore, the present findings provide scientific evidence for the nutritional potential, chemical composition, and biological activities of Trapa japonica Flerow anddemonstrate its potential use as a functional food forapplication in the pharmaceutical industry

경부 CT검사 시 선속경화인공물(Beam Hardening Artifact)에 의해 제 6번 7번 경추 및 추간판 등의 질환 및 그밖 에 해부학적 구조를 정확히 구분하기에 어려움이 있다. 경부 CT검사를 시행할 경우 자세의 변화방법을 적용한 견관절 의 방향과 위치에 따른 영상평가 및 커널값의 변화에 따른 영상평가를 통하여 선속경화 인공물의 원인을 알아보고 가 장 적절한 검사자세 및 Kernel값을 실험을 통하여 알아보고자 하였다. 경부 CT검사를 위해 내원한 환자 30명(2010년 7월1일 ~ 2010년 12월31일까지 내원한 환자)을 대상으로 Somatom Sensation 16(Siemens, Enlarge, Germany)장비 를 이용하였고, workstation은 AW 4.4 version(GE, USA)을 이용하였다.. 환자 자세는 견관절의 방향과 위치에 따라 세가지 자세로 변화를 주었으며 양쪽 팔을 편안하게 위치시킨 바로 누운 자세(group N), 왼쪽 팔을 거상 시킨 자세 (group S) 그리고 양손을 외 선위(eversion)시켜 최대한 아래로 내리는 자세(group P)로 견관절의 방향을 변화를 주 어 스캔을 시행하였고, 두 번째로 영상 재구성 방법을 이용하여 스캔 데이터에 커널값을 B10(very smooth), B20(smooth), B30(medium smooth), B40(medium), B50(medium sharp), B60(sharp), B70(very sharp)로 변화를 주어 재구성 하였다. 검사자세의 변화와 Kernel값의 변화를 주어 얻어진 영상 데이터를 이용하여 각 각의 노이즈 값 측정과 영상평가를 통하여 분석해보았다. 경부 CT검사 시 검사자세는 양손을 외선위(eversion)시켜 최대한 아래로 내 리는 자세(group P)로 하며, 커널 값은 B40(medium)또는 B50(medium sharp)으로 재구성할 경우 가장 적절한 자세 와 커널값으로 분석되어 임상에 적용 시 매우 유용할 것으로 사료된다.