피부를 대상으로 한 살균을 목적으로 하는 외용소독제 의 경우 식품 취급자에 오염된 미생물의 사멸 또는 제거 를 목적으로 활용될 수 있으며, 최근 개인위생에 대한 관 심 증가에 따라 제품 소비 증가와 제품 다양화가 두드러 지게 나타나고 있다. 살균 효능은 소독제의 핵심 품질 평 가 요소로서 수행 절차 및 조건에 따라 상이한 결과가 나 타날 수 있기 때문에 시험법의 효율성과 정확성을 높이기 위한 연구가 필요하다. 이에 본 총설논문에서는 주요 제 형별(겔형, 액제형, 와이프형) 시험법 개발 현황을 파악하 고 시험법별 특장점 분석 결과와 최근 관련 연구를 통하 여 제시된 시사점을 기반으로 향후 효능 평가 체계의 발 전 방향을 제시하고자 하였다. 인체 대상 시험법의 경우 시험 유형에 따라 소독제를 시험 대상 피부 표면에 처리 하는 조건이 다양화되어 있어 시험법 간 동등성에 대한 평가를 통해 소독제 제품의 성분이나 특성에 따라 최적의 시험 유형을 파악하고 그에 대응되는 적절한 평가 체계 및 관련 규제의 표준화의 필요성을 시사하였다. 특히 와 이프형 소독제의 경우 처리 방식이 미생물 제거 및 살균 에 직접적으로 영향을 미침에도 불구하고 피부에 노출하 는 손 대상 처리를 위한 사용 패턴의 표준화 사례가 부족 하였다. 한편 [전처리 - 소독제 노출 - 미생물 회수] 등 각 시험 절차별로 결과에 영향을 미치는 주요 결정 요인을 발굴하는 연구의 지속 수행을 통해 기존 시험법을 개선하고 신규 시험법을 개발하고자 하는 노력이 요구된다. 최 근 활발하게 개발되고 있는 ex vivo 시험법은 인체 시험 의 제한적인 연구 재현성과 같은 한계를 극복하면서도 인 간 피부 환경을 구현하기 위한 기술의 적용을 통해 연구 결과의 신뢰도를 확보할 수 있을 것으로 판단된다. 한편 손 피부를 대상으로 한 균총 연구 등 소독제 처리 전후 미생물의 특성과 분포 분석 관련 연구가 최근 다수 보고 되고 있어 이를 활용한 미생물 군집 단위의 소독제 효능 평가 시험법의 확립이 기대된다. 본 연구를 통해 제시된 소독제 효능 시험법의 현황 기반 발전 전략은 보다 효과 적인 개인위생 관리 확립을 통해 손을 통해 교차 오염되 는 미생물에 의한 감염성 질병 발생을 최소화하여 공중보 건 및 식품 안전성 향상에 기여할 수 있다.

This mini-review focused on the current advances in the development and the application of the antibacterial agents produced from probiotics strains against foodborne pathogens. Inhibitory activity of the pathogen growth could be achieved by co-culture with probiotics and/or the treatment of metabolites extracted from probiotics culture, whereas strain-dependent efficacy was mainly reported according to the bacterial species of the target pathogens. To overcome the limited antibacterial spectrum and the efficacy of the metabolites from probiotics, the recent research highlight the discovery of novel bioactive substances with broad range of the inhibitory activity of foodborne pathogens and mode-of-action which has not been reported as the major research goal. Moreover, understanding the distribution of functional and regulation genes coding the production of the antibacterial metabolites based on the bacterial genome analysis can provide the clues for the mechanisms of the pathogen control by using probiotics. Major strategies on the application of the genomics in this research area can be represented as follows: 1) functional annotation specialized for antimicrobial proteins, 2) assessment of the antibacterial effects followed by the general/functional annotation, 3) genome and metabolites analysis for the purification of antimicrobial proteins, 4) comparative genomics and the characterization of antimicrobial potential. Especially the relationship between phenotype-genotype based on genomic bioinformation with the results of the practical efficacy tests of bacterial metabolites as an active substance of disinfectants and/or preservatives can be expected to act as supportive research for broadening our knowledge regarding the key metabolic pathways associated with the production of antibacterials from probiotics.

It is well known that thermal plasma process has lots of advantages such as high temperature and good quality for synthesis of nano particles. In this research, we attempt the synthesis of nano unitary and composite powder (Ag, Mg-Al, Zr-V-Fe) using transferred thermal plasma. Nano particles of metal alloy, ranging from 20 nm to 150 nm, have been synthesized by this process.

The study is about variations in Carbonyl compounds concentration within apartment buildings according to pre-residence and residence. We consecutively investigated indoor air pollutants in 120 households in 6 cities at pre-residence and residence. Carbonyl compounds were collected using the 2,4-DNPH cartridge and were analyzed using HPLC. The carbonyls concentration of indoor air in the new apartments before occupation measured formadlehyde(76.0㎍/㎥), acetone(85.9㎛/㎥), acetaldehyde(13.8㎍/㎥). The carbonyls concentration of indoor air in the new apartments after occupation measured formadlehyde(233.1㎍/㎥), acetone(128.9㎍/㎥), acetaldehyde(29.8㎍/㎥), respectively. As a result, the mean concentration of carbonyl compounds within the pre-occupancy stage was lower than those of residence.

The results of particulate matters level and heavy metal concentration, which surveyed in Gwang-Yang, Dang-Jin steel industry area, are as follows; The PM2.5, PM10 of exposure area are 22.3μg/m3, 40.4μg/m3 each in Kum-Ho dong, and 28.1μg/m3, 51.5 each in Jung dong. The PM2.5, PM10 of control area are 16.4μg/m3, 29.5μg/m3 each in Bonggang-myeon. The level is higher in exposure area than control area. In case of Dang Jin, the concentration of PM10 and PM2.5 is higher in exposure area than control area (PM2.5-20.4μg/m3, PM10-39.2μg/m3). The Pb level of Dang Jin area is higher in exposure area(0.13μg/m3) than control area(0.1μg/m3), and both Gwang-Yang and Dang-Jin area lower level than the Guideline level of Korea EPA.

In this study, in order to analyze the air quality of the indoor environments of schools, we measured the indoor, outdoor and personal exposure concentration level of PM10 for 40 classrooms(20 old, 20 new) in chungnam area from June 22 to July 19 and from November 21 to December 30, 2003. 1. Old classrooms contained more dust than new classrooms; the average of respirable dust is 43.27 ㎍/㎥ for new classrooms while 53.38 ㎍/㎥ for old one. The exposure concentration level of respirable dust in new classrooms were in summer higher outdoors than indoors. The values were indoors 46.71 ㎍/㎥, outdoors 50.46 ㎍/㎥, and personal 41.62 ㎍/㎥. Meanwhile in winter indoors had a higher concentration level than outdoors, the values being indoors 39.11 ㎍/㎥, outdoors 34.86 ㎍/㎥, and personal 49.01 ㎍/㎥. 2. Cr concentration level within dust was slightly higher in summer indoors (101.50±32.10 ng/㎥) and outdoors (100.89±35.18 ng/㎥) than winter indoors (85.80±48.95 ng/㎥) and outdoors (74.43±38.93 ng/㎥), but in personal concentration level, winter was higher. The results of this research show insufficient understanding of health risks from indoor air pollution, and shows possible health problems to students from school indoor air pollution. As such, a logical and systematic education program for students about the importance of indoor air quality should be carried out. Also the results of PM10 concentration level measurements emphasize the need for regular measurements of indoor / outdoor and personal concentration level. New classrooms in particular needs to be used after measuring pollutants and safety, and requires installation of a ventilation device in all classrooms to improve air quality.

The concentrations of HCHO(formaldehyde), PM10(particulate matter), CO₂(carbon dioxide) and TBC(total bacteria counter) distribution in schools(Chung-Nam Area) were examined, and the results were compared with the recommended criterion of the administration law of indoor air. The subjects were an elementary school, a middle school and a high school in Chung-Nam area, and the concentration of TBC was examined by Single Stage Air Cascade Sampler, which applied the inertia collision catching method of 28.29L/min(flux) during 5 months from March, 2007 to July, 2007. The instrument(LD-3B, SIBATA Company)was used to examine PM10, by a light scattering method and a light transmission method. The instrument(Airboxx(KD Engineering) was used to examine CO₂. The instument(Z300XP(Environmental sensor)was used to examine HCHO. The result indicated that the PM10 average concentrations of the surveyed classrooms were 49 μg/m3 in Spring and 59 μg/m3 in Summer. The CO₂ average concentration of the surveyed schools were 576 ppm in the classroom and 527 ppm in the stateroom. The average concentration of TBC were 729 CFU/m3 in an elementary school, 401 CFU/m3 in a middle school, 381 CFU/m3 in a high school. The HCHO average concentration of the surveyed schools were 0.03 ppm in the classroom, 0.02 ppm in the stateroom.

연약지반의 점토퇴적물을 시멘트와 함께 생석회, 소석회, 석고, 고령토, 제올라이트, 규조토 등의 광물재료를 혼합하여 고화 반응시킨 후 양생시간에 따른 물성변화와 반응생성물을 조사하였다. 그 결과, 시멘트로는 포틀랜드 시멘트보다는 슬래그시멘트가 점토의 고화반응에 따른 강도발현이 큰 것으로 나타났다. 그리고 슬래그시멘트와 함께 각종 광물재료와 고화 반응시킨 실험결과에서는, 석고가 가장 높은 강도발현을 나타냈다 따라서 슬래그시멘트와 석고에 대해 이들의 혼합비를 달리하여 고화 실험을 수행하였다. 그 결과, 슬래그시멘트 70%, 석고 30%의 비율에서 가장 좋은 강도발현을 보이는 것으로 나타났다. 이 실험의 고화반응물에서는 에트린자이트 등의 반응생성물이 포함되었다. 이것은 석고가 슬래그시멘트의 효율적인 수화반응을 촉진시켜서 높은 강도 발현에 기여 한 것으로 생각된다. 이러한 실험 결과는 연약지반의 안정화 처리에 유용한 자료로 이용될 것으로 보인다.