In this study, changes in the microbial ecosystem of the Yeongheungdo island coastal waters were investigated for five years to collect basic data. To evaluate the influence of distance from the coast on the microbial ecosystem, four sites, coastal Site (S1) and 0.75, 1.5, and 3 km away from the coast, were set up and the changes in physicochemical and biological factors were monitored. The results showed seasonal changes in water temperature, dissolved oxygen, salinity, and pH but with no significant differences between sites. For nutrients, the concentration of dissolved inorganic nitrogen increased from 6.4 μM in April-June to 16.4 μM in July-November, while that of phosphorus and silicon phosphate increased from 0.4 μM and 2.5 μM in April-June to 1.1 μM and 12.0 μM in July-November, respectively. Notably, phosphorus phosphate concentrations were lower in 2014-2015 (up to 0.2 μM) compared to 2016-2018 (up to 2.2 μM), indicating phosphorus limitation during this period. However, there were no differences in nutrients with distance from the coast, indicating that there was no effect of distance on nutrients. Phytoplankton (average 511 cells mL-1) showed relatively high biomass (up to 3,370 cells mL-1) in 2014-2015 when phosphorus phosphate was limited. Notably, at that time, the concentration of dissolved organic carbon was not high, with concentrations ranging from 1.1-2.3 mg L-1. However, no significant differences in biological factors were observed between the sites. Although this study revealed that there was no disturbance of the ecosystem, further research and more basic data on the microecosystem are necessary to understand the ecosystem of the Incheon.

PURPOSES : The objective of this study is to develop an optimal placement of the overturning prevention device based on a 3D object model during the development of the girder-type overturning prevention device for the remote control construction of bridges METHODS : Based on existing construction methods and literature research, a detailed design model of a phase-optimized conduction prevention device is presented. The 3D-object model data, optimal placement, and an operating range of the equipment developed for the remote control construction of bridge girders were considered. RESULTS : The shape of the overturning prevention device was selected taking into account the dimensions and operating range of numerous devices developed for the remote control construction of the bridge. In particular, the detachable device was chosen to allow a trouble-free construction considering the spacing between the pier cap of the bridge and the girders. The overturning prevention device presented based on this 3D object model will be manufactured as a mock-up and used for a demonstration project on remote control bridge construction. CONCLUSIONS : This will enable easy and economical construction as this development will support remote control construction of bridge girders to avoid accidents such as workers falling from high places like piers.

In 2020, the diversity of benthic macroinvertebrate communities was investigated in the Sohan stream, an ecological and landscape conservation area, and the results were compared with the previous research conducted in 2011. In total, 42 species (two phyla, three classes, and seven orders) were found in the Sohan stream. Species richness and abundance sharply decreased at all sampling sites because of Typhoons Haishen and Maysak in 2020, which had a direct impact on the stream. In the functional feeding group, the ratio of collector-gatherers was the highest at all sampling sites. However, during the autumn season, the shredder ratio increased from 13.4% to 42.4% in the uppermost stream site. Compared with the diversity of benthic macroinvertebrates surveyed in 2011, a total of 53 species (two phyla, three classes, and eight orders) were found. The percentage of species richness and abundance of Ephemeroptera, Plecoptera, and Trichoptera was more than 50% both in 2011 and 2020. Only the richness of Ephemeroptera was significantly different between them (2011: 2.9 and 2020: 6.7). In this study, the abrupt changes of species richness and abundance in benthic macroinvertebrate were not observed before and after the designation of an ecological and landscape conservation area. However, it is necessary to monitor benthic macroinvertebrates in order to confirm that biodiversity is continuously maintained long after the designation of the ecological and landscape conservation area.

유해 남조류 Microcystis 종에 의한 녹조현상은 매년 빈번하게 일어나며, 이로 인한 수자원의 질적 변화와 먹는 물 확보에 문제가 발생하고 있다. Microcystis 종에 의한 피해를 막고자 개발된 naphthoquinone (NQ) 유도체 물질의 장점 분석을 위해 국내에서 빈번히 사용되는 황토를 이용한 microcosm 실험을 하고자 하였다. 그 결과, NQ 40, NQ 2-0 물질은 선행 연구 결과와 동일하게 유해 남조류 Microcystis 종을 선택적으로 99.9%, 99.6% 제어했으며, 식물플랑크톤 종 다양성을 증진시켰다. 그러나, 황토를 처리한 실험구는 실험 초기 일시적인 조류 제어효과를 보인 후 다시 증가하였으며, 유용 조류를 포함한 모든 식물플랑크톤에게 적용되어 다른 조류의 성장은 이루어지지 않았다. 뿐만 아니라, NQ 물질을 처리한 처리구는 비생물학적, 생물학적요인 모두 영향을 미치지 않았으며, 대조구와 유사한 경향이 관찰되었다. 따라서, 최종적으로 개량된 유해 남조류 제어물질 NQ 2-0은 높은 살조효과, 선택적 제어효과, 저독성, 자연분해에 의한 비잔류성 뿐만 아니라, 편의성 및 경제성까지 갖춘 새로운 살조물질로서 현재까지 개발된 살조물질 보다 현장 생태계 적용에 가장 적합한 친환경 녹조제어 물질이라고 판단된다.

Bloom of small centric diatom Stephanodiscus is quite occasional in winter season in temperate freshwater ecosystems. It often leads to degradation of water quality and affects the quality of supplied drinking water. In a previous study, we have found that naphthoquinone (NQ) 4-6 derivate is an effective tool for efficient mitigation of natural S. hantzschii blooms. In the present research, polylactide (PLA) and agar foam were used as immobilized agent for NQ 4-6 to improve the efficiency of NQ 4-6 compound releasing process for its application under various field conditions. Mesocosm experiments at 10 ton scale suggested that the abundance of S. hantzschii was continuously increased in the control and upon treatment of the mesocosm with immobilized NQ 4-6 from PLA and agar foam. Their algicidal activities were 78.8% and 77.1%, respectively, on S. hantzschii after 10 days. In the mesocosm experiments, the dynamics of biotic (bacteria, HNFs, ciliates, zooplankton) and abiotic (water temperature, dissolved oxygen, pH, conductivity, nutrients) factors remained unaffected. They exhibited similar trends in the control and treatment groups. Therefore, the immobilized NQ 4-6 from PLA and agar foam has potential to be used as an alternative algicidal substance to effectively mitigate natural S. hantzschii blooms under various field conditions. In addition, it not only can be used to control S. hantzschii, but also is an effective technique. The immobilized NQ 4-6 showed stable controlled release in desired system.

For several decades, lactic acid bacterium (Lactobacillus graminis: LAB) has been generally recognized as safe. To develop the pan-environmental bio-control agent, algicidal activity of the live LAB cell and its culture filtrate (CF) was examined against Microcystis aeruginosa. LAB cells perfectly lysed M. aeruginosa within 3 days, while the CF had a less effect than the live cells, approximately 78% inhibition of algal growth during a same culture period. The concentration of microcystin in alone culture of M. aeruginosa was 7.1 μg L-1, but gradually increased and leach 158.5 μg L-1 on 10 days. However, LAB cells clearly decreased the microcystin by 10.3 μg L-1 in the same period, approximately 93.5%. CF of LAB showed a strong algicidal activity over 75% between pH 2-7, 91.3% by the treatment of proteinase K, 87.8% by below 3 kDa in particle size, and 75.3% by heat treatment, respectively. Of five solvents, fractions of CF passed through solvents diethyl ether and ethyl acetate showed an obvious algicidal activity in the algal-lawn test. Among 5 fractions purified by silica-gel TLC plate, two spots showed a most strong removal activity on M. aeruginosa. Another analysis of GC indicate that CF contained six representative fatty acids. Even though most of these substance have been known as an anti-algal substance against M. aeruginosa, oleic acid is the most effective. These results suggested that the culture filtrate or specific substances, like a fatty acids, in comparison with live L. graminis can be a successful and eco-friendly agent to control Microcystis bloom.