Arthrobacter sp. L-3이 생성하는 glucose isomerase를 DEAE-cellulose column chromatography법으로 2단계 NaCl농도 구배로 용출함으로서 순수분리하였다. 이것이 SDS-acrylamide gel electrophoresis상에서 단일띠를 보임으로서 매우 잘 분리되었음을 알 수 있었다. Glucose isomerase의 K_m값과 V_max값이 각각 0.175M, 0.29(㎎/㎖/min)로 얻어졌다. 한편, SDS-acrylamide gel electrophoresis와 Sephadex-G100-50에 의한 gel filtration으로부터 분자량이 각각 42, 000과 180, 000으로 얻어져, 이 효소는 분자량이 42, 500인 4개의 subunit로 구성되었음을 알 수 있었다.
The objective of this study was to propse the way that could enlarge the course in life for the graduates of Department of Food and Nutrition in Junior College. This study was carried out through the analysis of statistical data and questionaries gathered from professors of Department of Food and Nutrition in Junior College and from dieticians of industry, school and hospital. The results of this study could be summarized as follows. 1. The cultivation number of dieticians is too excessive in comparison with the need, and so it is necessary for the colleges to restrict the new establishment of Department of Food and Nutrition for a time, and for the government to tighten the employment system of dieticians. 2. The employment of dieticians will be extended to such fields as school, hospital, public health center and counsel office of nutrition to act as a nutritional educator or a medical server in the future, and consequently the colleges which cultivate dieticians should improve the curriculum to agree with this. 3. It is necessary for the government to renovate the system of dietician arrangement to such a way as establishes a new rule in which additional dietician should be employed in accordance with the feeding number in industry and hospital, as restricts the position-combine of a dietician with a cook in a large feeding institute, as gives the qualification of a nutritional instructor only to the diatician at public health center, as gives the qualification of a nutrition -teacher to the dieticians who completed the course of study for the teaching profession and then allows them to act as a nutrition-teacher in school. 4. The way to give a higher grade of educational opportunity should be given to the graduates of Department of Food and Nutrition in Jonior College for the supplement of dietician`s faculty.