In this study, we investigated the variation in free sugars, organic acids, antioxidant activity and anti-inflammatory effect of Solanum nigrum Linne fruits according to harvest time. Four kinds of free sugars (fructose, glucose, sucrose, maltose) were detected in S. nigrum fruit, and the free sugar contents varied significantly with harvest time. Organic acid content of S. nigrum fruit showed the highest in malic acid and acetic acid, and the highest content of total organic acids was found in S. nigrum fruit harvested on October 18th and October 25th. For the total polyphenol content, S. nigrum fruit harvested on October 18th was the highest. The strongest DPPH and ABTS radical scavenging activity was showed in S. nigrum fruit harvested on October 11th and October 18th. The anti-inflammatory activity and antioxidant effects were the highest in the ethanol extract from S. nigrum fruit collected on October 18th and October 11th. Thus, it seems the best to harvest of S. nigrum fruit harvested on October 11th and October 18th.

Background : Recently, hair loss, which has been regarded as a mere means of middle-aged men due to stress and environmental pollution. The market for hair loss in Korea is about four trillion won and it is growing continuously. It is mainly made by mixing natural extracts such as medicinal plant. The purpose of this study was to investigate the effects of ethanol extracts of Houttuynia cordata Thunb. whole plant and Calendula officinalis L. flower extracts on the growth of fibroblasts, dermal papilla cells and lipid precursors, I want to try to make a materialization. Methods and Results : The cytotoxicity of each sample extracts treated with 50%, 100%, and 500 μg to fibroblasts, cell-viability were 107.3%, 109.6%, and 128.2%, respectively. The cytotoxicity of each sample to the dermal papilla cells was not observed. And the lipid differentiation of the lipogenic precursor cells which regulates the hairegeneration by secretion of the platelet derived growth factor. The 70% ethanol extracts of H. cordata whole plant and C. officinalis flower were showed promotes the hair growth activity. The lipolysis rate was significantly increased with increasing treatment concentration Conclusion : As a result of this study, in-vitro hair growth activity of herbal medicines for hair treatment material development was not shown to be toxic to each cell. And 70% ethanol extract of H. cordata whole plant stimulated lipid precursor cells inducing differentiation. As a result, the 70% ethanol extracts of H. cordata whole plant and C. officinalis flower have potential to developing hair-related product.

Background : Polygonum multiflorum Thunb. is a herbaceous perennial belonging to the Polygonaceae family. And is an herbal medicine which can be used as a raw material for food, which is excellent in immunity enhancement, vocalization and blood transfusion. The purpose of this study was to expand the utility of the P. multiflorum. Also, we fermented P. multiflorum by mushroom mycelial, and analyzed for general components and amino acids before and after fermentation Methods and Results : The moisture content of P. multiflorum and fermented P. multiflorum by mushroom mycelial (FPM) were 7.35% and 59%, respectively. The crude protein content did not show a significant difference between the two samples, crude fat, ash and crude fiber content of FPM were lower than P. multiflorum. The content of soluble nitrogen free extract of P. multiflorum (79.78%) was significantly higher than FPM (31.05%). Sixteen kinds of amino acids were detected in P. multiflorum, and the major amino acid was determined the arginine. The content of arginine and glutamic acid were 586.67 ㎎%, and 283.78 ㎎%, respectively. Sixteen kinds of amino acids were detected in FPM, and the major amino acids were determined the arginine (654.68 ㎎%) and threonine (591.18 ㎎%). The total amino acid contents of P. multiflorum and FPM were 3,469.03 ㎎%, and 3,630 ㎎%, respectively. Conclusion : The content of crude fat, ash, crude fiber, and soluble nitrogen free extract of FPM were lower than the P. multiflorum, and the major amino acids were different in two samples. Total amino acid content of FPM was higher than the P. multiflorum. As the mushroom fermentation progresses, it is confirmed that the amino acid content is increased, and it is expected to develop the product using the P. multiflorum fermented with mushroom mycelial.

In this study, we investigated the variation in free sugars, amino acids, antioxidant activity, and anti-inflammatory activity of Solanum nigrum Linne based on harvest time. Major amino acids identified by HPLC analysis were proline, histidine, and serine. The highest content of total amino acids were found in S. nigrum aerial parts and roots harvested on July 10th and August 10th. Four kinds of free sugars (fructose, glucose, sucrose, maltose) were detected in S. nigrum, and the free sugar content varied significantly with harvest time. The fructose content of S. nigrum decreased with as harvest time increased. The total polyphenol content of S. nigrum was highest in those harvested on August 30th. The antioxidant activity of ethanol extract from S. nigrum collected at different harvest times were measured by DPPH and ABTS radical scavenging assays. The anti-inflammatory activity of these extracts were assayed via nitric oxide suppression in C6 glioma cells with a lipopolysaccharide (LPS)-induced inflammatory response. The anti-inflammatory activity and antioxidant effects were the highest in the extract from S. nigrum collected on August 30th. Good correlations were observed between antioxidant and anti-inflammatory activities in ethanol extract of S. nigrum roots harvested on August 30th.

배합사료 제조 시 우리나라는 95% 이상을 수입에 의존하고 있으나 한약재 부산물을 활용할 경우 공급처에서는 수입에 의존하는 단미사료 원료를 대체가능하며 악화된 조사료 시장 및 축우 경영에 많은 도움이 될 것이다. 또한 수요처에서는 한약재 부산물을 처리함으로써 주변환경 개선 및 연간 2억원 정도의 절감효과를 기대할 수 있고 절감된 비용을 활용하여 약품개발을 위한 연구 인력을 보충할 수 있다. 한약재 부산물을 활용하여 첫째 TMR 사료 원료인 볏짚 및 수입 단미사료 원료를 대체 하는 것을 목표로 한다. 한약재 부산물을 최적의 조건으로 발효시켜 유효기간을 늘리고 축우의 기호성을 증가시키며 자동화 TMR 배합 설비를 구축하여 많은 양의 한약재 부산물을 처리한다. 둘째 유용 미생물인 바실러스 균, 유산균, 효모균을 자연생태계에서 분리 동정한 후 효소, 항균력, 발효력, 상호 공존력을 평가하고 소화제 한약재 부산물을 유용미생물로 발효시켜 건조, 분쇄 후 제과분과 적정 비율로 혼합 후 친환경 고품질 생균제를 개발한다. 개발된 생균제를 위탁기관을 통해 양어･양계에 적용하여 생체 내 비특이적 면역반응과 항균력을 입증하여 전라북도 뿐만 아니라 전국적인 사업망을 통해 안정적인 생균제 판매망을 구축하고자 한다. 또한 기존 공업용 미생물 배지를 대체할 수 있는 한약재 부산물을 활용한 미생물 배지를 개발하여 음수첨가제 및 발효제를 개발하고자 한다.

신안산 손바닥선인장의 수분함량은 열매 71.18%, 줄기 74.85%로 나타났고, 조단백질 함량은 줄기가 3.48%로 높았으며, 조섬유 또한 줄기가 2.58%로 열매 1.54%보다 높은 함량을 보였고, 조회분, 조지방은 열매와 줄기 모두 비슷한 함량을 보였다. 신안산 손바닥선인장의 유리당은 fructose, glucose, sucrose으로 구성되어 있었으며 maltose는 소량 검출되었고, 유기산은 tartaric acid, malic acid