The introduction of stress up-regulated genes into heterologous plants is found to confer stability trait to plants in otherwise hostile environments. The soybean cold inducible factor-1 gene(SCOF-1) encoding a zinc finger transcription factor was introduced into Populus alba L.(white poplar) by co-culture with Agrobacterium tumefaciens GV3101 that carried the binary vector, pGA643/SCOF1. The putative P. alba transformed plants were selected on Murashige and Skoog medium supplemented with 2.22μM 6-benzylaminopurine, 1.18mM carbenicillin, and 28.47μM hygromycin. The integration of SCOF-1 into the P. alba genome was also established by polymerase chain reaction(PCR), and the expression of the gene was confirmed by reverse transcription PCR. After exposure for 4h at 4℃ followed by 48h recovery at 27℃, the transgenic plants completely recovered to normal growth status. The chlorophyll content in transgenic plants was higher than that in wild type plants after chilling treatment. However, the moisture content and total phenolics was also not significantly different in transgenic and wild type plants after cold treatment.

The influence of the culture media on the growth and catechins and caffeine production was investigated in adventitious root cultures of tea tree. The growth rate of adventitious roots was higher than that of solid medium. Growth rate of adventitious roots was noted to be optimal in N6 liquid medium. Yields of (-)-epicatechin gallate (ECG) and caffeine were the highest when adventitious root cultures were maintained in Nitsch and B5 liquid medium, respectively. The production of (-)-epicatechin (EC) and (-)-epigallocatechin gallate (EGCG) from adventitious roots was maximal when cultured in 1/2MS liquid medium. The adventitious root extracts of tea tree produced on catechins as EC (20.77 mg/g) and EGCG (1.84 mg/g) in 1/2MS medium; EGC (24.39 mg/g) and caffeine (12.97 mg/g) in B5 medium and (-)-epigallocatechin (ECG) (2.16 mg/g) in Nitsch liquid medium.

In this study, 160 tea tree (Camellia sinensis L.) lines were classified by caffeine content using colorimetric methods. Among them, caffeine-rich lines (HR-78, HR-137, HR-82 and HR-123) and poor lines (HP-85, HP-88, HP-19, and HP-131) were selected. To know the difference in morphological and genetic characters between caffeine-rich and poor lines, we used leaf/shoot growth and RAPD methods. Cluster pattern of morphological characters (leaf width, leaf length, leaf area and shoot length) showed that shoot length was longer in caffein-rich lines than in -poor lines. In genetic analysis, amplified DNA bands having various sizes were detected in RAPD analysis where 30 random primers were used. However, the discriminated primer set that distinguish caffein-rich tree line from -poor lines was not found. These results can be used as the basic data to determine the morphological and genetic differences among caffein-rich and -poor lines

제주도와 남부지방에서는 콩잎을 채소용으로 이용하여 고기와 생선을 쌈사서 먹는데 이용해 왔으며, 주로 지역의 재래종이나 일본에서 도입된 ‘단파흑두’를 재배해왔다. 그러나, 이들은 잎 크기가 균일하지 않아 상품성에 문제가 있었다. 그러므로, 쌈용 콩잎의 채소용 품종개발이 필요하다. 콩잎의 채소용 ‘청엽1호’ 품종이 2009년 식량원에서 개발되었다. ‘청엽1호’는 내도복 다수성 품종인 ‘일품검정콩’과 대립이며 잎이 큰 ‘대황콩’을 교배한 품종이다. ‘청엽1호’는 꽃색이 백색, 검정색 종피, 황색 자엽, 구형의 종자모양, 100립중이 35.8g인 대립종이다. 또한, 콩잎의 채소용 적성으로 개화기가 늦어 채엽기간이 길고 잎이 크고 둥근 난형, 잎색이 연한 녹색, 내도복성, 마디의 수가 많으며, 줄기의 두께가 두꺼워 채엽시 잘 부러지지 않는 특성을 가진다. ‘청엽1호’의 비가림재배시 잎 수량은 헥터당 18.44톤으로 대비품종인 ‘단파흑두’보다 6% 높았다. 콩잎의 기능성 성분으로 이소플라본 함량이 잎 g당 809 ㎍으로 대비품종보다 63% 높았다. 이상과 같이 ‘청엽1호’는 콩잎을 채소용으로 이용하기에 매우 적합한 품종이다.