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        검색결과 128

        1.
        2024.06 KCI 등재 구독 인증기관 무료, 개인회원 유료
        Background: Porcine embryonic development is widely utilized in the medical industry. However, the blastocyst development rate in vitro is lower compared to in vivo . To address this issue, various supplements are employed. Extracellular vesicles (EVs) play the role of communicators that carry many bioactive cargoes. Additionally, the contents of EVs can vary on the estrous cycle. Methods: We compared the effects of adding EVs derived from porcine uterine fluid (UF), categorized as non-EV (G1), EVs in estrus (G2) and EVs in diestrus (G3). After in vitro culture (IVC) was performed in three different groups, cleavage rate and blastocyst development rate were examined. In addition, glutathione (GSH) and reactive oxygen species (ROS) levels were measured 2 days after activation to assess oxidative stress. Results: Using NTA and cryo-TEM, we confirmed the presence of EVs with sizes ranging from 30 nm to 200 nm, that the particles were suitable for analysis for analysis. In IVC data, the highest cleavage rate was observed in G2, which was significantly different from G1 but not significantly different from the next highest, G3. Similarly, the highest blastocyst development rate was observed in G2, which was significantly different from G1 but not significantly different from the next highest, G3. Conclusions: These results indicate that estrus derived EVs contain biofactors beneficial for early blastocyst development, including GSH which protects the blastocyst from oxidative stress. Additionally, although diestrus-derived EVs are expected to have some effect on blastocyst development, it appeared to be less effective than estrus-derived EVs.
        4,000원
        2.
        2024.02 KCI 등재 구독 인증기관 무료, 개인회원 유료
        이 연구에서는 Inception V3, SqueezeNet(local), VGG-16, Painters 및 DeepLoc의 다섯 가지 인공지능(AI) 모 델을 사용하여 차나무 잎의 병해를 분류하였다. 여덟 가지 이미지 카테고리를 사용하였는데, healthy, algal leaf spot, anthracnose, bird’s eye spot, brown blight, gray blight, red leaf spot, and white spot였다. 이 연구에서 사용한 소프트웨 어는 데이터 시각적 프로그래밍을 위한 파이썬 라이브러리로 작동하는 Orange3였다. 이는 데이터를 시각적으로 조작하여 분석하기 위한 워크플로를 생성하는 인터페이스를 통해 작동되었다. 각 AI 모델의 정확도로 최적의 AI 모 델을 선택하였다. 모든 모델은 Adam 최적화, ReLU 활성화 함수, 은닉 레이어에 100개의 뉴런, 신경망의 최대 반복 횟수가 200회, 그리고 0.0001 정규화를 사용하여 훈련되었다. Orange3 기능을 확장하기 위해 새로운 이미지 분석 Add-on을 설치하였다. 훈련 모델에서는 이미지 가져오기(import image), 이미지 임베딩(image embedding), 신경망 (neural network), 테스트 및 점수(test and score), 혼동 행렬(confusion matrix) 위젯이 사용되었으며, 예측에는 이미 지 가져오기(import image), 이미지 임베딩(image embedding), 예측(prediction) 및 이미지 뷰어(image viewer) 위젯 이 사용되었다. 다섯 AI 모델[Inception V3, SqueezeNet(로컬), VGG-16, Painters 및 DeepLoc]의 신경망 정밀도는 각 각 0.807, 0.901, 0.780, 0.800 및 0.771이었다. 결론적으로 SqueezeNet(local) 모델이 차나무 잎 이미지를 사용하여 차 병해 탐색을 위한 최적 AI 모델로 선택되었으며, 정확도와 혼동 행렬을 통해 뛰어난 성능을 보였다.
        4,200원
        4.
        2023.11 구독 인증기관·개인회원 무료
        The disposal of spent nuclear fuel (SNF) in a deep geological repository (DGR) is a widely accepted strategy for the long-term sequestration of radiotoxic SNF. Ensuring the safety of a DGR requires the prediction of various reactions and migration behaviors of radionuclides (RNs) present in SNF within its geochemical surroundings. Understanding the dissolution behaviors of mineral phases harboring these RNs is crucial, as the levels of RNs in groundwater are basically linked to the solubility of these solid phases. Accurate measurements of solubility demand the use of welldefined solid materials characterized by chemical compositions and structures. Herein, we attempted the synthesis of sklodowskite, a magnesium-uranyl (U(VI))-silicate, employing a twostep hydrothermal synthetic approach documented previously. Subsequently, we subjected this synthesized sklodowskite to various analytical techniques, including powder X-ray diffraction (pXRD), scanning electron microscopy/energy dispersive X-ray spectrometry (SEM/EDX), and vibrational spectroscopies (FTIR and Raman). Based on our findings, we confidently identify the obtained mineral phase as sklodowskite (Mg[UO2SiO3OH]2·5H2O). This identification is primarily based on the similarity between its pXRD pattern and the reference XRD pattern of sklodowskite. Furthermore, the measured infrared and Raman spectra show the vibrational modes of UO2 2+ and SiO4 4- ions, particularly within the 700~1,100 cm-1 region, which support that the synthetic mineral has a characteristic layered uranyl-silicate structure of crystalline sklodowskite. Finally, we utilized synthetic minerals to estimate its solubility up to about three months in a model groundwater, where the dissolved species composition is analogous to that of granitic groundwater from the KAERI Underground Research Tunnel. In this presentation, we will present in detail the results of spectroscopic characterizations and the methodology employed to assess the solubility of the U(VI)-silicate solid phase.
        5.
        2023.09 KCI 등재 구독 인증기관 무료, 개인회원 유료
        This study was conducted to assess the genetic variability and correlation of phenotypic characteristics in 12 tomato (Solanum lycopersicum L.) genotypes including 11 WorldVeg and one commercial variety (Pusa Ruby) in Terai (plain) region of Nepal in 2021–2022. This experiment was laid out in a randomized complete block design with three replications. The phenotypic traits, including days to 50% flowering, plant vigor and height, fruit number/plant, fruit yield, fruit weight and diameter, fruit firmness and fruit pericarp thickness, and total soluble solids (TSS) content of the fruits, were studied. Analysis of variance revealed significant differences among the genotypes for all the traits except for plant vigor. The genotype of AVTO1705 resulted the highest fruit yield (2.9 kg/plant) than Pusa Ruby, a commercial check (0.5 kg/plant). The phenotypic coefficient of variation (PCV) was higher than the genotypic coefficient of variation (GCV) for all the traits and PCV values were maximum for the number of fruits, fruit yield, and fruit weight. High PCV, GCV, and genetic advance (GA) were observed for yield, fruit weight, and plant height, respectively, indicating the additive gene effect. High heritability for fruit yield/plant and plant height inferred the phenotypic selection for their genetic improvement. Fruit yield was significantly (P<0.05) positively correlated with the fruit number and fruit weight, and direct selection of these traits are reliable for yield improvement in tomato.
        4,000원
        6.
        2023.05 구독 인증기관·개인회원 무료
        As the importance of radioactive waste management has emerged, quality assurance management of radioactive waste has been legally mandated and the Korea Radioactive Waste Agency (KORAD) established the “Waste Acceptance Criteria for the 1st Phase Disposal Facility of the Wolsong Lowand Intermediate-Level Waste Disposal Center (WAC)”, the detailed guideline for radioactive waste acceptance. Accordingly, the Korea Atomic Energy Research Institute (KAERI) introduced a radioactive waste quality assurance management system and developed detailed procedures for performing the waste packaging and characterization methods suggested in the WAC. In this study, we reviewed the radioactive waste characterization method established by the KAERI to meet the WAC presented by the KORAD. In the WAC, the characterization items for the disposal of radioactive waste were divided into six major categories (general requirements, solidification and immobilization requirements, radiological, physical, chemical, and biological requirements), and each subcategories are shown in detail under the major classification. In order to satisfy the characterization criteria for each detailed item, KAERI divided the procedure into a characterization item performed during the packaging process of radioactive waste, a separate test item, and a characterization item performed after the packaging was completed. Based on the KAERI’s radioactive waste packaging procedure, the procedure for characterization of the above items is summarized as follows. First, during the radioactive waste packaging process, the characterization corresponding to the general requirements (waste type) is performed, such as checking the classification status of the contents and checking whether there are substances unsuitable for disposal, etc. Also, characterization corresponding to the physical requirements is performed by checking the void fraction in waste package and visual confirmation of particulate matter, substances containg free water, ect. In addition, chemical and biological requirements can be characterized by visually confirming that no hazardous chemicals (explosive, flammable, gaseous substances, perishables, infectious substances, etc.) are included during the packaging process, and by taking pictures at each packaging steps. Items for characterization using separate test samples include radiological, physical, and chemical requirements. The detailed items include identification of radionuclide and radioactivity concentration, particulate matter identification test, free water and chelate content measurement tests, etc. Characterization items performing after the packaging is completed include general requirements such as measuring the weight and height of packages and radiological requirements such as measurements of surface dose rate and contamination, etc. All of the above procedures are proceduralized and managed in the radioactive waste quality assurance procedure, and a report including the characterization results is prepared and submitted when requesting acceptance of radioactive waste. The characterization of KAERI’s radioactive waste has been systematically established and progressed under the quality assurance system. In the future, we plan to supplement various items that require further improvement, and through this, we can expect to improve the reliability of radioactive waste management and activate the final disposal of KAERI’s radioactive waste.
        10.
        2022.05 구독 인증기관·개인회원 무료
        To obtain confidence in the safety of disposal facilities for radioactive waste, it is essential to quantitatively evaluate the performance of the waste disposal facilities by using safety assessment models. Thus, safety assessment models require uncertainty management as a key part of the confidencebuilding process. In application to the numerical modelling, the global sensitivity analysis is widely employed for dealing with parametric and conceptual uncertainties. In particular, the parametric uncertainty can be effectively reduced by minimizing the uncertainty of critical parameters in the safety assessment model. In this paper, the numerical model of each step disposal facility (Silo, Near surface, and Trench type) at Wolsong Low and Immediate Level Waste (LILW) Disposal Center is designed by using a two-dimensional finite element code (COMSOL Multiphysics). In order to determine the critical parameters for non-adsorbed nuclides such as H-3, C-14, Tc-99, we introduced the variance-based sensitivity analysis methodology of the global sensitivity analysis. In the case of Silo type, the density of waste is highly sensitive to the total leakage quantity of all nuclides. Additionally, the initial nuclide concentration of H-3 was identified as another important parameter of H-3. On the other hands, the mass transport coefficient showed a high contribution in C-14 and Tc-99. In other types of disposal facilities, the leaking properties of H-3 are significantly affected by the amount of infiltration water. However, C-14 and Tc-99 were found to be more sensitive to the density of waste.
        12.
        2021.12 KCI 등재 구독 인증기관 무료, 개인회원 유료
        In the present study, we examined the effect of different lecithin concentrations on spermatozoa characteristics after freeze-thawing. Hanwoo semen was collected from one bull and divided into five groups (tris-citric acid semen extender with 0, 0.1, 0.25, and 0.5% lecithin groups as well as a 20% egg yolk group). Semen extender with 20% egg yolk was used as control treatment. After the freeze-thawing of semen, spermatozoa motility, motility parameters, viability, acrosomal membrane integrity, mitochondrial membrane potential, and plasma membrane integrity were examined. In experiment 1, the effect of different lecithin concentrations on spermatozoa motility and associated parameters was examined. The 0.1% lecithin-treated spermatozoa showed greater fast progressive motility (%) in addition to higher VCL (μm/s), VSL (μm/s), and VAP (μm/s) when compared to other lecithin concentration groups and controls. In experiment 2, the effect of different lecithin concentrations on spermatozoa viability was examined. The 0.1% and 0.25% lecithin addition groups (55.4±7.3 and 51.7±11.2%) exhibited similar viability compared to the control group (54.1±12.6%). In experiment 3, the effects of different lecithin concentrations on viability, acrosomal membrane integrity, and mitochondrial membrane integrity of spermatozoa were examined. The percentage of live spermatozoa with an intact acrosome and high mitochondrial membrane potential in the 0.1% lecithin group was not significantly different compared to the control group (31.2±13.3 vs. 30.5±10.9%). In experiment 4, the effect of different lecithin concentrations on the plasma membrane integrity of spermatozoa was examined. The percentage of spermatozoa with a normal plasma membrane was similar between the 0.1% lecithin and control groups (31.2±13.3 vs. 30.5±10.9%). In conclusion, we suggest that semen extender supplemented with 0.1% lecithin can replace 20% egg yolk without reducing spermatozoa quality.
        4,000원
        15.
        2021.09 KCI 등재 구독 인증기관 무료, 개인회원 유료
        기대수명의 증가나 산업구조의 다변화 등 여러 요인에 의해 평생교육의 중요성이 부각되고 있으며, 우리나라에서도 국가사업의 일환으로 평생교육정책이 확대되면서 성인학습자들에 관한 연구가 증가하고 있다. 본 연구는 교수자와의 관계를 통한 지지가 중년기 여성인 성인학습자의 삶의 질에 미치는 영향을 살펴보고자 하였다. 특히 중년기 여성이 대학생활을 경험함에 있어서 느끼는 만족이 이 두 변수 사이에서 어떠한 역할을 하는지 분석하여 그 영향력을 규명하고, 차후 평생교육정책의 수립 및 평생교 육실천현장에 유용한 정보를 제공하는데 목적이 있다. 이를 위하여 경남에 위치한 2년제 및 4년제 대학에 재학 중인 중년기 여성을 대상으로 설문조사한 255부의 설문지를 분석에 활용하였다. 본 연구의 연구결과는 다음과 같다. 첫째, 중년 여성학습자의 대학생활만족에 교수자 지지는 통계적으로 유의한 정(+)의 영향을 미치는 것으로 나타났다. 둘째, 교수자 지지는 중년 여성학습자의 삶의 질에 통계적으로 유의한 정(+)의 영향을 미치는 것으로 나타났다. 셋째, 중년 여성학습자의 대학생활만족은 교수자 지지와 삶의 질과의 관계에 매개효과가 있는 것으로 나타났다. 분석결과를 바탕으로 중년여성의 평생 교육 활성화 및 삶의 질 향상을 위한 제언을 하였다.
        6,100원
        16.
        2021.06 KCI 등재 구독 인증기관 무료, 개인회원 유료
        In the present study, we examined if deep uterine artificial insemination (DUAI) can improve the pregnancy rate of artificial insemination (AI) using epididymal spermatozoa (ES) in Hanwoo cattle. The estrus cycles of 88 Hanwoo cows were synchronized, and 17 cows were artificially inseminated using the DUAI method with ES, 20 cows were artificially inseminated via the uterine body (BUAI) method with ES, and as a control, 51 cows were inseminated by using the BUAI method with ejaculated spermatozoa from 1 proven bull after frozen thawing. The pregnancy rate of the DUAI method (58.8%) was higher than that of the BUAI method (25.0%, p = 0.0498). The motility of ES was examined immediately after thawing and after 3 and 6 h of incubation. The rapid progressive sperm motility of the control group was significantly higher than that of the ES group immediately after thawing and after 3 and 6 h of incubation (p < 0.05). The straight line velocity and average path velocity of the ES group after 6 h of incubation were significantly lower than those in the control group (p < 0.05). The linearity and amplitude of lateral head of ES were lower than those at 6 h (p < 0.05). The flagellar beat cross frequency and hyperactivation of ES were lower than the control spermatozoa immediately after thawing and at 3 h (p < 0.05). These motility parameters suggested that ES had a low motility and fertilization ability compared to the control spermatozoa. After frozen-thawing and 3 h of incubation, the percentage of live spermatozoa with intact acrosomes in the ES was significantly lower than that in ejaculated spermatozoa (p < 0.05). Our findings suggested that the DUAI method can overcome the low pregnancy rate of ES, despite the low motility, viability, and fertilization ability of ES.
        4,000원
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