In this paper, a simple, cost-effective, and efficient electrochemical sensor for molecular imprinting melatonin was established. The molecular imprinted films were formed by in situ electrochemical polymerization using molecular imprinting technology. The modification method, modification time and other parameters of the electrode were optimized. Under optimized conditions, the sensor responds to melatonin concentration in a linear range of 0–100 μM. The detection limit was 0.171 μM. In addition, the sensor has little response to interfering substances, such as uric acid, vitamin B6, vitamin C, and glucose, and can be tested in real samples. The recoveries were 98.73–101.60%.

경상남도농업기술원 화훼연구소에서 2021년 화색이 연황 색이며 화심이 녹색인 미니 절화용 거베라 품종 ‘크림쿠키’ (Cream Cookie)를 육성하였다. ‘크림쿠키’ 품종은 2014년 황색 미니 ‘Sun City’를 모본으로, 백색 미니 ‘Blandy’를 부 본으로 인공교배를 실시하여 육성된 품종이다. 2021년까지 생육 및 개화 특성검정과 기호도 조사를 실시하였으며 ‘크림 쿠키’의 생육 및 개화특성을 대조품종인 ‘Sun City’와 비교하 였다. ‘크림쿠키’는 연황색(RHS 4C) 꽃잎과 녹색 화심을 가진 반겹꽃 거베라 품종이다. 화폭이 7.1cm인 작은 꽃이며, 화경 장은 58.9cm였다. 화경 직경은 상부 0.4cm, 하부 0.7cm 였 다. 외부설상화의 길이는 2.8cm이며 폭은 0.8cm였다. 개화 소요일수는 65.7일로 ‘Sun City’에 비하여 18.8일 빨랐으며, 첫 개화시 엽수는 22.4매였다. 연간 주당 절화수량은 102.3 본으로 ‘Sun City’의 82.0본에 비하여 20.3본이 많았다. 절 화수명은 17.8일로 ‘Sun City’보다 4.1일 더 길었다. ‘크림쿠 키’는 화폭이 7.1cm의 미니 품종으로 연간 주당 100본 이상 절화 생산이 가능하다. 또한 수확 후 꽃 캡 씌우기, 꽃목 보강 등 추가적인 작업을 생략할 수 있어 생산비 절감이 가능하므 로 농가보급 확대가 기대된다.

Biomass-derived porous carbon is an excellent scientific and technologically interesting material for supercapacitor applications. In this study, we developed biomass-derived nitrogen-doped porous carbon nanosheets (BDPCNS) from cedar cone biomass using a simple KOH activation and pyrolysis method. The BDPCNS was effectively modified at different temperatures of 600 °C, 700 °C, and 800 ℃ under similar conditions. The as-prepared BDPCNS-700 electrode exhibited a high BET surface area of 2883 m2 g− 1 and a total pore volume of 1.26 cm3 g− 1. Additionally, BDPCNS-700 had the highest electrical conductivity (11.03 cm− 1) and highest N-doped content among the different electrode materials. The BDPCNS-700 electrode attained a specific capacitance of 290 F g− 1 at a current density of 1 A g− 1 in a 3 M KOH electrolyte and an excellent longterm electrochemical cycling stability of 93.4% over 1000 cycles. Moreover, the BDPCNS-700 electrode had an excellent energy density (40.27 Wh kg− 1) vs power density (208.19 W kg− 1). These findings indicate that BDPCNS with large surface areas are promising electrode materials for supercapacitors and energy storage systems.

To investigate the effect of the catalyst and metal–support interaction on the methane decomposition behavior and physical properties of the produced carbon, catalytic decomposition of methane (CDM) was studied using Ni/SiO2 catalysts with different metal–support interactions (synthesized based on the presence or absence of urea). During catalyst synthesis, the addition of urea led to uniform and stable precipitation of the Ni metal precursor on the SiO2 support to produce Ni-phyllosilicates that enhanced the metal–support interaction. The resulting catalyst upon reduction showed the formation of uniform Ni0 particles (< 10 nm) that were smaller than those of a catalyst prepared using a conventional impregnation method (~ 80 nm). The growth mechanisms of methane-decomposition-derived carbon nanotubes was base growth or tip growth according to the metal–support interaction of the catalysts synthesized with and without urea, respectively. As a result, the catalyst with Ni-phyllosilicates resulting from the addition of urea induced highly dispersed and strongly interacting Ni0 active sites and produced carbon nanotubes with a small and uniform diameter via the base-growth mechanism. Considering the results, such a Ni-phyllosilicate-based catalyst are expected to be suitable for industrial base grown carbon nanotube production and application since as-synthesized carbon nanotubes can be easily harvested and the catalyst can be regenerated without being consumed during carbon nanotube extraction process.

The inclusion of conductive carbon materials into lithium-ion batteries (LIBs) is essential for constructing an electrical network of electrodes. Considering the demand for cells in electric vehicles (e.g., higher energy density and lower cell cost), the replacement of the currently used carbon black with carbon nanotubes (CNTs) seems inevitable. This review discusses how CNTs can contribute to the development of advanced LIBs for EVs. First, the reason for choosing CNTs as a conducting agent for the cathode is discussed in terms of energy density. Second, the reinforcing effect of CNTs on the anode is described with respect to the choice of silicon as the active material. Third, the development of water-based cathode fabrication as well as dry electrode fabrication with aid of CNTs is discussed. Fourth, three technical hurdles, that is, the price, dispersion issue, and entrapped metal impurities, for widespread use of CNTs in LIBs are discussed.

In 2018, media reports raised issues related to radon released from building materials used as finishing materials in apartment houses. Accordingly, related ministries recommended not to use materials with a radiation index value exceeding 1. In order to calculate the radioactivity index, not only 226Ra producing radon (222Rn) but also 232Th and 40K radioactivity concentrations are required. To determine the concentration of the radionuclide, 40K is measured by a single gamma ray of 1,460.8 keV. And the 228Ac used to measure 232Th mainly utilizes gamma rays of 911.2 keV. However, 228Ac does not appear as a single peak unlike 40K, and appears as multiple peaks at various energies. Among them, gamma rays are emitted at a intensity of 0.83% at 1,459.2 keV, which is likely to interfere with 40K. Therefore, what is actually measured at 1,460.8 keV is theoretically a compound peak of 40K and 228Ac. Because the probability of emission at 1,459.2 keV (0.83%) is low, a low concentration of 232Th will result in little 40K radioactivity error. However, samples containing a high concentration of 232Th overestimate the 40K radioactive concentration, so correction is required. In this study, the IAEA standard substance (IAEA-RGTh-1) ontaining 232Th of actual high concentration was analyzed, and the results of the analysis without correction of 40K were compared and verified. As the 40K correction method, the 911.2 keV gamma-ray of 228Ac was used as the reference peak to separate the peak of 228Ac (1,459.2 keV) from the 40K (1,460.8 keV) mixed peaks. And, the coefficient value obtained by subtracting the peak of 228Ac (1,459.2 keV) from the 40K (1,460.8 keV) mixed peak was set to a pure peak of 40K and the radioactivity concentration was calculated therefrom. As a result of calculating the IAEA-RGTh-1 reference material without correction, it was confirmed that the 40K value was overestimated by about 38 times. If a measurement beyond the MDA of 40K is generated by 228Ac radioactivity because the 40K correction constant is not applied, there is an error in determining that there is 40K radioactivity. However, even if 40K radioactivity is overestimated due to the high concentration of 232Th, the degree to which this effect contributes to the radioactivity index is very small. However, as an analyst, 40K radioactivity correction should be made for more accurate analysis.

Although canine brucellosis has been known to be an important re-emerging zoonosis, the pathophysiological mechanisms of Brucella canis infection remains clues to be solved. Different culture models, single and co-culture models, were constructed with canine epithelial cells, D17 and macrophage, DH82 to investigate the induction of immune responses in in vivo B. canis infection. Expression of genes related with induction of immune responses, Th1, Th2 and Th17, was compared in the two different models after the bacterial infection. In this study, expression of cytokine genes, IL-1β, IL-5, IL-6, IL-10, IL-23, and TNF-α was quantified in the DH82 at different time points using RT-qPCR in the two different culture systems after the infection. Cytokine genes related with Th1, IL-1β and TNF-α and Th17, IL-6 and IL-23 were expressed with time-dependent manners in the both systems (p<0.05). However, increase of Th2-related cytokine genes expression was not detectable in the both systems by comparison with control. The expression of Th1 and Th17 related cytokine genes was earlier in single cell culture than those in co-culture model (p<0.05). In general, amounts of the expressed genes were shown higher in single cell model than those in co-culture models. This study indicate that Th1 and Th17-associated immune responses are central to B. canis infection in dogs. In addition, it suggests a specific role of epithelial cells in the B. canis infection in vivo, which should resolved in the further study.

Methyl bromide (MB) has been still routinely used in quarantine fumigation on imported citrus, although there had been issuing chronic inhalation toxicity to fumigators and related workers as well as phytotoxic damages after fumigation. Ethyl formate (EF), is the only option to replace MB in terms of its safety for consumers (food additive and naturally occurred) and worker with higher threshold level limit (TLV = 100 ppm). Its application technology also provide cost effectiveness, good commercial practice in terms of application time (< 10 min) for 40 ft container. The replacement of MB with EF is recommended not to fumigate with hazardous and phasing-out MB on imported oranges.

식품 중 곰팡이 이물에 대한 연구를 위하여 식품 제조 및 유통/보관현장에서의 곰팡이 오염수준 및 주요 곰팡이류를 조사하였다. 측정은 식품유형별 총 9장소 (젓갈류, 식초류, 쌀류, 밀가루류, 냉동만두류, 면류, 과자류, 육가공류, 김치류)와 유통/보관현장 총 8장소(물류 창고)에서 진행하였고, 각 생산라인에서 부유곰팡이, 표면 곰팡이 오염도를 조사하였다. 측정결과, 육가공류 생산현장에서 부유곰팡이 오염도가 가장 높게 측정되었으며, 김치류 생산현장에서의 부유곰팡이 오염도가 가장 낮게 측정되었다. 설비 및 벽면에서의 표면 곰팡이 오염도의 경우, 식품 제조 및 유통/보관 현장에 관계없이 거의 검출되지 않았다. 현장에서 검출된 주요곰팡이를 조사한 결과, Penicillium sp., Aspergillus sp., Cladosporium sp., 종이 우점종 곰팡이로 조사되었다.

식품 중 곰팡이 이물에 대한 연구를 위하여 식품 제조 및 유통/보관 현장에서의 곰팡이 오염수준 및 주요 곰팡이류를 조사하였다. 측정은 식품유형별 총 8장소(과자류, 젓갈류, 식초류, 빵류, 쌀류, 밀가루류, 냉동만두류, 생면류)와 유통/보관현장 총 8장소(물류창고)에서 진행하였고, 각 생산라인에서 부유곰팡이, 표면 곰팡이 오염도를 조사하였다. 측정결과, 젓갈류 생산현장에서 부유곰팡이 오염도는 5.5 × 102 CFU/m3 로 가장 높게 측정되었으며, 쌀류 생산현장에서 의 부유곰팡이 오염도는 38 CFU/m3 로 가장 낮게 측정되었다. 설비 및 벽면에서의 표면 곰팡이 오염도의 경우, 식품 제조 및 유통/보관 현장에 관계없이 거의 검출되지 않았다. 현장에서 검출된 주요곰팡이류를 조사한 결과, 총 42종의 곰팡이가 검출되었고, 그 중 Aspergillus sp., Penicillium sp., Cladosporium sp. 종이 우점종 곰팡이로 조사되었다. 기존 문헌에서 알려진 식품제조현장에서의 곰팡이 종과 공통적으로 발견된 종은 총 8종이고 새롭게 발견된 종은 총 34종으로 조사되었다.