토마토 온실에서 담배가루이 (Bemisia tabaci) 유인을 위한 작물에 따른 선호도를 조사하였다. 피망, 토마토, 오이, 가지의 4 종류의 작물을 아크릴케이지에 같이 넣어두고 담배가루이 성충 30쌍을 방사하여 선호도 및 산란수를 조사하였다. 담배가루이 성충은 오이(약 40%)와 가지(약 40%)에서 가장 선호하였으며, 토마토(10%)와 피망(10%)의 선호도는 낮았다. 기주에 따른 담배가루이의 성충 30쌍의 산란수는 오이에서 485개로 가장 높았으며 가지(250), 토마토(131), 피망(126)의 순으로 조사되었다. 토마토 온실에 가지묘를 정식하였을 경우에는 토마토에서는 주당 약 20마리의 담배가루이가 조사되었으나 가지에서는 주당 400∼500마리의 담배가루이가 조사되어 토마토 온실에서 가지를 이용한 담배가루이 유인이 가능함을 알 수 있었다.

In the current study, a total of 102 common Todarodes pacificus squid caught in the East Sea were investigated for parasitological research. The results revealed that 33 (32.35%) out of 102 squid were infected by Nybelinia surmenicola, the mean intensity was 5.58 parasites per squid, and the maximum abundance was 11. Morphological analysis using a field emission scanning electron microscope showed the characteristic features of N. surmenicola. Molecular identification based on the 28S rRNA gene confirmed the isolated parasite as N. surmenicola, while phylogenetic analysis revealed that N. surmenicola isolated in this study was clustered with N. surmenicola isolated from Japan. This is the first report of phylogenetic characterization of N. surmenicola isolated from Korea.

1분 간격 자동 잎면적 분석을 통해 온도에 따른 담배거세미나방 유충의 섭식과 발육을 조사하였다. 20, 25, 30℃의 세 온도에서 피망 잎을 배지에 부착하여 담배거세미나방 유충에 제공하였고 하루에 한번내지 두번 새로운 잎을 갈아주었다. 30℃에서 담배거세미나방 유충은 탈피 직후 섭식을 즉시 시작하여 1회 섭식은 10~15분 지속하고, 60~90분 간격으로 섭식을 하였다. 이러한 섭식 행동은 약 30~50시간 지속되며 탈피 직전 10~16시간 동안은 섭식이 중단되었다. 탈피 후 앞서의 섭식 패턴은 다음 영기에서 동일하게 반복되었다. 25℃에서 담배거세미나방 유충의 영기 내 섭식 기간은 약 32~53시간 지속되며 탈피 전 비섭식 기간은 16~22시간 지속되었다. 20℃에서는 영기 내섭식 기간은 60~133시간으로 크게 증가하고 비섭식 기간도 11~38시간으로 다른 온도에 비해 길어졌으며 다른 온도와 달리 7령충까지 발육하였다. 2령충에서 고치 형성 전까지의 발육 시간은 20℃에서는 39046분(27.1일)이 소요되었고 25℃에서는 17626분(12.2일), 30℃에서는 14168분(9.8일)으로 조사되었다. 각 온도별 유충의 섭식량은 영기에 따라 지수적으로 증가하였다.

본 연구에서는 2015년부터 2017년까지 전국 29개의 지역에서 갈색날개매미충(Ricania spp.) 난괴를 채집하여 갈색날개매미충 알기생봉(Telenomus spp.)의 발생 분포 및 생태적 특성을 조사하였다. 강원도를 제외한 내륙의 19 지역에서 갈색날개매미충의 난괴로부터 알기생봉이 출현하였다. 알기생봉에 기생된 구례지역 채집 난괴를 실내조건 (25 ± 1℃, 40~60% RH, 16L:8D)에서 관찰한 결과, 알기생봉의 평균발육기간(day)이 알 2.6, 유충 5.4, 번데기 15.0 였으며 알부터 성충 우화 전까지 20.0였다. 6월부터 8월까지 일주일 단위로 채집된 구례지역의 갈색날개매미충 미부화 월동 난괴에서 알기생봉은 7월부터 난발육을 시작하여 8월중순에 우화하였다. 우화한 알기생봉은 갈색날개매 미충 신성충의 알에 기생하여 20일 후 재발생 하였고 다시 산란하여 알 상태로 월동하였다. 실내조건에서 알기생봉은 점등 후 우화하기 시작하여 2-4시간 후에 가장 높은 우화율을 보였고 점차 감소하여 소등 이후에는 전혀 우화하지 않았다.

약제 처리에 따른 담배거세미나방(Spodoptera litura) 유충의 섭식 행동 및 반응을 조사하기 위해 1분 간격으로 잎면적을 자동 측정하였다. 담배거세미나방 유충의 섭식 행동은 섭식기와 비섭식기가 뚜렷하게 구분되었으며, 섭식기가 끝나면 비섭식기를 거쳐 탈피를 하고 다시 섭식기에 들어갔다. 1회 섭식 지속 시간은 약 15분으로 조사되었고, 영기 내 평균 섭식 회수는 약 27.5회로 섭식 지속 시간과 마찬가지로 영기에 따른 차이는 없었다. 약제 처리는 피망 잎을 Buprofezin methoxyfenozyd과 BT (Bacillus thuringiensis) 500ppm 약액에 10초간 침지 처리하였다. Buprofezin methoxyfenozyd를 처리 하였을 경우 섭식 시간은 평균 8~9분으로 감소하였고, 치사 전까지 섭식량은 각각 176mg으로 조사되었다. 하지만 BT제를 처리 시에는 담배거세미나방 유충은 치사하지 않았다.

The cowpea aphid Aphis craccivora Koch (Hemiptera: Aphididae) is an important pest on several species of legumes. We investigated the development periods and survivorship of A. craccivora nymph, and longevity and fecundity of A. craccivora adult. The study was conducted at five constant temperatures of 10, 15, 20, 25, and 30°C, respectively. The lower developmental threshold (LDT) and thermal constant (K) of A. craccivora nymph stage were estimated by linear regression. Higher temperature threshold (TH) and lower temperature threshold (TL) were calculated by biophysiological model. Developmental completion of A. craccivora nymph stage was described using a two-parameter Weibull distribution model. Life table parameters were estimated by the jackknife method.

The squid Todarodes pacificus is a commercially important fishery species in East Asia. As East Asians consume raw or lightly cooked squids, there has been growing concern about parasitic infections associated with squid consumption. In the current study, five squids caught in the East Sea were sampled for parasitological research from the biggest fishery market in Seoul, Republic of Korea. They were dissected and examined for parasites using a light microscope. Proteocephalus sp. was isolated and identified using field emission scanning electron microscopy, PCR, and sequencing. Given that Proteocephalus spp. can be affected by water temperature and geographical characteristics, further research on Proteocephalus spp. with respect to changes in water temperature in the East Sea is important.

바다에 기름오염 사고가 발생하면 여러 가지 방제 방법 중 물리적 회수 방법을 우선적으로 사용하고 유처리제는 최후 수단으로 고려하는 경향이 있다. 유처리제는 수중으로 기름이 신속히 분산되도록 하여 해수면으로부터 제거하는 방법이다. 해수면으로부터 신속히 기름을 제거하는데 대한 유처리제의 효용성은 널리 증명되어 왔으나 아직도 대부분의 국가들은 해양환경에 미치는 독성을 우려하여 적극적인 사용을 하지 않고 있는 실정이다. 보고된 자료에 의하면 유처리제 사용 후 수중생물에 대한 독성은 발견되지 않았으며, 유처리제와 혼합된 기름이 기 름 그 자체보다 독성이 더 크게 나타나지 않았다. 멕시코만 기름유출 사고 시 미국 정부와 BP사는 최대한 해안에 기름이 도달하지 않는데 중 점을 두고 해수면뿐만 아니라 수중의 기름에 대해서도 유처리제를 사용하였다.

Background : The purpose of this study was to evaluate methods to high quality medicinal vegetables, thereby increasing farm income. This study investigated the effects of different green manures crop and application culture stable foliage production in Peucedanum japonicum Thunberg. Methods and Results : Method of application with green manure crop were conducted under four condition compose to control (non application), single cropping in barley, hairy vetch and mixed cropping in hairy vetch (60%) + barley (40%)culture. Sowing dates of green manure crop were October 14 in 2014. Transplanting with Jeonam Yeosu varieties in Junel 28 by growing pot seedling for 45 days in 2015. Method of application were standard application (N-P-K-Compost applied at 20-12-10-600 ㎏․10 a−1). Plants were spaced 10 ㎝ apart in rows 10 ㎝ apart with open cultivation. Conclusions : The utilization effect of nitrogen supplied by green manure crop was highest with the hairy vetch by 21.1% in single cropping and hairy vetch + barley by 29.8% in mixed cropping. Organic matter, available phosphate, K2O, CaO, and MgO of soil increased in plots with the mixed cropping hairy vetch + barley culture. Non application < barley < hairy vetch < hairy vetch + barley in mixed cropping, aerial part as a result were so many such amount of growth as number of leaf per plant, etc., green leaf yields is untreated control plot (1,876 kg․10 a−1) compared to 7% from using barley culture, hairy vetch culture 10% higher and hairy vetch + barley culture increased to 15% of the most.

Background : Recently, there is a urgent demand for development of new varieties with enhanced resistance to various biotic/abiotic stresses. In order to develop ginseng varieties with such traits, systematic breeding program and comprehensive field studies are prerequisite. Methods and Results : ‘Cheonmyeong' seeds were collected in 2008 from the farmer field of Buyeo. Physiological investigation and propagation were conducted from 2009 to 2011. It was given the name 'Eumseong No. 8 through the observed yield trial from 2012 to 2013 and local adaptability was carried out from 2014 to 2015. All phenotypes including agronomic characteristics, seed yield, and physiological response to biotic/abiotic stresses were investigated according to the ginseng GAP and UPOV guidelines. Yellow-red leaf and pink berry at maturing stage were observed. The time of emergence, flowering and berry maturity of the ‘Cheonmyeong’ were faster than those of ‘Chunpoong’. Stem length of ‘Cheonmyeong’ was shorter than that of ‘Chunpoong’, whereas stem diameter was thicker than that of ‘Chunpoong’. Main root length was shorter but main root diameter is thicker than that of ‘Chunpoong’. Number of seeds of ‘Cheonmyeong’ was fewer than that of ‘Chunpoong’ but 1,000-seeds weight and stratification rate were higher than those of ‘Chunpoong’. The yield performance of this variety was 661 kg/10 a in local adaptability test for two years, which is 22% higher than that of ‘Chunpoong’. ‘Cheonmyeong’ showed strong resistance to phytophthora blight, mulberry mealybug and nematode and moderate resistance to alternaria blight. ‘Cheonmyeong’ did almost not occur yellow spot of aerial part and rusty skin of root, show moderate resistance at high temperatures. Conclusion : Our study demonstrated that ‘Cheonmyeong’ is an ideal variety with heavier root weight and enhanced stress resistance and contribute will enhance biotic/abiotic stress resistance and increase the farmers' income.

Background : Water uptake and flow across cellular membranes is a fundamental requirement for plant growth and development, and plant water status is important not only for plant growth under favorable conditions but also for ability of a plant to tolerate adverse environmental conditions. Thus identification of plasma membrane water channel genes (aquaporins) in ginseng provides extensive information for functional studies and the development of markers for salinity stress tolerance. Methods and Results : For salinity treatment, the plants were grown for 4 weeks in culture medium gelled with 0.8% Phytoagar, and the old media were replaced with the fresh medium containing NaCl at 0, 50, 100, 200 and 400 mM, respectively. The samples for stress treated and non-stressed plants were collected from 6h to 72h, and frozen immediately into liquid nitrogen. According to the sequence information from the assembled transcripts, four primer pairs were designed from the aquaporin gene regions. In order to determine the pattern of aquaporins expression in ginseng seedlings to salinity stress, we conducted semi-quantitative RT-PCR. Conclusion : A tonoplast intrinsic protein 1 (TIP1)-type aquaporin is not only believed to be essential for plant life, but also to be beneficial for growth under salinity stress. Therefore, a deeper understanding of aquaporin genes in ginseng will be essential for crop improvement, which could help us to understand the molecular genetic basis for the ginseng genetic improvement and also provide the functional genetic resources for selective breeding and transgenic research.

Background : This study was carried out to understand the effect of seedling weight (SW) on growth and flowering in Panax ginseng. Methods and Results : The testing materials were Chunpoong (CP), Yunpoong (YP) and Jakyeongjong (JK). The increase of seedling (1yr) weight led to an increase in ratio of flowering plant and in number of flower per plant. The seed setting rate of two year-old plant (CP, YP, JK) increased with increase of SW at the planting time (PT) and number of flower per plant of three year-old plant (CP, YP) increased also. In the two year-old plant (JK), the ratio of three leaves per plant was 8.8, 19.6, 31.0, 42.0, 44.7 and 58.2%, respectively, in the SW of >0.6, 0.6~0.8, 0.8~1.0, 1.02~1.2, 1.2~1.4 and 1.4g<. The growth of ginseng plant was good with increase of SW at the PT. Conclusion : There was a highly positive correlation between seedling weight and flowering characteristics.

Background : Correct identification of Panax species is important to ensure food quality, safety, authenticity and health for consumers. This paper describes a high resolution melting (HRM) analysis based method using internal transcribed spacer (ITS) and 5.8S ribosomal DNA barcoding regions as target (Bar-HRM) to obtain barcoding information for the major Panax species and to identify the origin of ginseng plant. Methods and Results : A PCR-based approach, Bar-HRM was developed to discriminate among Panax species. In this study, the ITS1, ITS2, and 5.8S rDNA genes were targeted for testing, since these have been identified as suitable genes for use in the identification of Panax species. The HRM analysis generated cluster patterns that were specific and sensitive enough to detect small sequence differences among the tested Panax species. Conclusion : The results of this study show that the HRM curve analysis of the ITS regions and 5.8S rDNA sequences is a simple, quick, and reproducible method. It can simultaneously identify three Panax species and screen for variants. Thus, ITS1HRM and 5.8SHRM primer sets can be used to distinguish among Panax species.