Fish assemblage of total 325 of Korean peninsula estuaries were surveyed to analyze the characteristics of community structure and diversity by sea areas for three years from 2016 to 2018. The scale (stream width) of Korean estuaries were various (14~3,356 m), and 68.9% of all estuaries showed salinity of less than 2 psu. Total 149 species classified into 52 families of fish were identified, and the dominant and sub-dominant species were Tribolodon hakonensis (relative abundance, RA, 12.5%) and Mugil cephalus (RA, 9.5%), respectively. The estuary of the Korean Peninsula had different physical and chemical habitat environments depending on the sea area, and accordingly, fish community structure also showed statistically significant differences (PERMANOVA, Pseudo-F=26.69, P=0.001). In addition, the NMDS (nonmetric multidimensional scaling) results showed the patterns that indicating fish community difference by sea areas, even though low community similarity within sea area (SIMPER, 21.79~26.39%). The estuaries of east sea areas were distinguished from the others in the aspects of which, the higher importance of migratory fishes and endangered species, and that of brackish species were characterized at south sea estuaries. However, the estuaries of west sea showed higher importance of species that have a relation with freshwater (primary freshwater species, exotic species), which is the result that associating with the lower salinity of west sea estuaries because of the high ratio of closed estuaries (78.2%). The SIMPER analysis, scoring the contribution rates of species to community similarity, also showed results corresponding to the tendency of different fish community structures according to each sea area. So far, In Korea, most studies on fish communities in estuaries have been conducted in a single estuary unit, which made it difficult to understand the characteristics of estuaries at the national level, which are prerequisite for policy establishment. In present study, we are providing fish community structure characteristics of Korean estuaries in a national scale, including diversity index, habitat salinity ranges of major species, distribution of migratory species. We are expecting that our results could be utilized as baseline information for establishing management policies or further study of Korean estuaries.

A new WRKY transcription factor gene was isolated by ESTs screening from a cDNA library of suspension cultured cells of Sweet potato (Ipomoea batatas). The 2,285 bp cDNA fragment, IbWRKY, was sequenced, from which a 505 amino acid residue protein was deduced. A search of the protein BLAST database identified significant similarity to other plant WRKY31 protein sequences. RT-PCR analysis showed expression patterns of IbWRKY31 in various intact tissues and suspension cultured cells of Sweet potato, and in leaves exposed to different stresses. The IbWRKY31 gene was highly expressed in suspension cultured cells. In leaf tissues, IbWRKY31 showed strong expression during salicylic acid and methyl jasmonate treatments. Expression of IbWRKY31 was also induced under various abiotic stress and pathogen infection conditions, such as wounding, H2O2, MV, PEG, NaCl, and bacterial pathogen infection. These results suggest that IbWRKY31 is involved in plant responses to various stress conditions, such as abiotic stresses and pathogen infection through a defense signaling pathway.

본 연구는 버섯산업의 현황 및 개선과제에 대한 분석을 바탕으로 계층적 분석방법(AHP)을 적용하여 개선과제의 우선순위를 도출하였다. 버섯산업의 4개 부문 내 11개 개선과제를 선정하고, 버섯산업 관련 전문가 32명에 대한 설문조사를 통하여 실증분석을 진행하였다. AHP 분석결과 버섯산업의 부문별 우선순위는 수출·마 케팅부문이 1위, 가공부문이 2위, 유통부문이 3위, 그리고 생산부문이 4위의 순으로 나타났다. 세부적인 개선과제에서는 품질 및 가격 경쟁력 강화가 1위, 다양한 제품 개발에 대한 지원 확대가 2위, 그리고 버섯의 국내가격 안정이 3위의 순으로 우선순위가 나타났다. 한편 신선도 유지를 위한 포장재개선, 재배시설 현대화, 저장비·물류비 지원 확대 등은 우선순위가 상대적으로 낮게 나타났다. AHP분석에서 1순위로 나타난 ‘품질 및 가격 경쟁력 강화’를 위해서는 국내 연구기관의 연구개발(R&D)에 대한 지원 확대가 필요하다. 그리고 생산농가들도 버섯의 품질 향상을 위한 적극적인 교육 참여 및 안전성 확보를 위한 노력을 기울여야 할 것이다. 또한 소비자 선호 조사를 통해 기능성 버섯 품종 개발 등 다양한 품종의 개발 및 생산이 필요하다. AHP분석에서 2순위로 나타난 ‘다양한 제품 개발에 대한 지원 확대’를 위해서는 국내외 소비자의 특성에 맞는 제품 개발이 필요하다. 그리고 트렌드(trend)를 반영한 신상품 및 기존 제품에 대한 품질 개선의 노력이 필요하다. 또한 수출국별로 버섯의 레시피(recipe) 개발 등 가공산업의 부가가치를 높일 수 있도록 적극적인 정책 지원이 필요하다. AHP분석에서 3순위로 나타난 ‘국내가격 안정’을 위해서는 생산단계에서 버섯 출하를 조절할 수 있는 출하조절 기구를 만들어 시기별, 품목별 생산량을 조정하여 과잉생산으로 인한 가격 하락을 막아야 한다. 또한 버섯이 과잉 생산될 경우 국내 물량을 신속하게 해외로 수출함으로써 국내 가격에 영향이 없도록 출하와 수출 간의 긴밀한 협력도 필요하다. 버섯산업의 종합적인 발전을 위해서는 부문별로 나누어 어느 한 부문만의 중요성을 강조하기는 어렵다. 버섯의 생산, 가공, 유통, 수출·마케팅이 유기적으로 연결되어 있으므로 어느 한 부문의 집중적인 개선 및 지원도 필요하지만, 산업 전반을 거시적으로 파악하여 전반적으로 개선하는 노력도 병행되어야 할 것이다.

청계천에 서식하는 어류 군집의 공간적 변이와 환경과의 관계를 측정하기 위해, 청계천 내 6개 지점을 대상으로 2년간 (2014~2015년) 이화학적 요인, 서식처 환경, 어류 군집을 조사하였다. 어류 군집의 공간적 변이는 지점-종 군 집 데이터 메트릭스를 기반으로 한 베타다양성 분석을 통해 정량적으로 제시하였다. 또한 청계천 내 전체 군집 변이 값 (베타다양성)과 함께 각각의 지점이 청계천 전체 베타다양도에 기여하는 값 (LCBD, Local Contribution to Beta Diversity)도 계산하였다. 데이터 분석의 기반이 되는 지점-종 군집 데이터 테이블은 출현-비출현, 풍부도, 헤링거 변환 값의 세 가지 형태로 적용하였고, 해당 데이터 형태에 따른 베타다양성과 지점별 변이 영향을 각각 계산하여 비교하였다. 헤링거 변환을 통해 계산된 베타다양성 값은 출현-비출현 정보나 풍부도를 바탕으로 한 분석보다 큰 값을 보여주어 공간적 변이를 가장 잘 보여주는 것으로 나타났다. 각 지점별 군집 변이 기여도 (LCBD)는 출현-비출현 정보와 해링거 변환을 통한 분석이 유사한 경향을 보여주었다. 자료의 정규성을 가지기 어려운 어류 군집 자료의 경우 풍부도를 이용한 공간 변이 분석은 적절하지 않은 것으로 판단된다. 추가적으로 다양한 환경 요인 및 군집 지수와 베타다양성 기여도 값의 관계를 상관분석을 통해 나타내었다. 해당 지점의 알파다양성 지수와 베타다양성 기여도가 높은 음의 상관관계를 보였고 이는 선행 연구와 유사한 결과이다. 본 연구에 적용한 방법은 매트릭스 형태의 자료를 대상으로 베타다양성 계산과 지점별 군집 변이 기여도를 수치화하는 데 유용한 것으로 나타났다.

Endoplasmic reticulum (ER) stress is well known as a suppressor in osteoblast differentiation and activating transcription factor 3 (ATF3) could be induced by a various extracellular signals including cytokines, hormones, DNA damage. Up to date, although the role of ATF3 have been studied, the function of ATF3 in osteoblast differentiation is still not clear yet. Our study showed that expression level of ATF3 could be incresed by tunicamycin which is ER stress inducer in preosteoblasts. BMPs, which are secreted by osteoblasts, can be important regulators in osteogenic differentiation. The stress-responsive transcription factor ATF3 is a negative regulator of osteoblast differentiation in MC3T3-E1 cells. In this study, we verified that BMP2-stimulated osteoblast differentiation could be inhibited by over-expressed ATF3 through regulating alkaline phosphatase (ALP) expression and activation.

The ambrosia beetle, Xyleborinus saxesenii Ratzeburg, infests physiologically stressed apple and peach trees in Korea. Dispersing females utilize the degradation product ethanol and host-related volatiles to locate and colonize new host trees. We examined the extent to which 12 chemicals emitted from fruit trees act synergistically with ethanol to attract X. saxesenii. The addition of benzaldehyde to ethanol significantly increased beetle attraction, although benzaldehyde was not attractive by itself. The addition of α-pinene, ethyl butyrate, ethyl isovalerate, limonene, 3-methyl-1-butanol, ethyl tiglate, aromadendrene, vanillin, 2-butanol, styrene, or ethyl 3,3-dimethylacrylate to ethanol had no effect on beetle attraction. In a dose-response test, the addition of 5–50% benzaldehyde doses synergistically increased the number of beetle captures; however, trap catches did not increase as the benzaldehyde dosage increased. The synergistic influence of benzaldehyde on beetle response to ethanol was lower in early spring than in late summer to early fall, probably because synthetic benzaldehyde emissions from field lures were overwhelmed by background levels of natural benzaldehyde emitted from peach twigs in the flowering stage.

버섯과 배지에 피해를 주는 긴수염버섯파리와 원예작물의 뿌리를 가해하는 작은뿌리파리 같은 버섯파리류의 성충 발생예찰에 끈끈이트랩이 사용되고 있으며 끈끈이트랩을 이용한 예찰은 살충제 및 친환경 방제 수단의 투입을 결정하기 위한 중요한 측도이기도 하다. 다양한 색상의 끈끈이트랩은 간편하고 빠르게 설치할 수 있기 때문에 버섯파리류의 성충을 비롯한 비행 능력이 있는 대부분의 해충 예찰에 이용된다. 버섯파리류 성충 예찰에는 황색 끈끈이트랩이 가장 효과적이며, 예찰뿐만 아니라 대량유살을 통한 방제 목적으로 이용되기도 한다. 버섯재배사와 원예작물 재배지에 발생하여 피해를 주는 버섯파리류 성충의 효과적인 예찰 및 방제를 위한 끈끈이트랩의 최적 설치위치를 검토한 결과,양송이와 느타리버섯은 2단 베드에, 톱밥표고버섯은 베드 위 100 cm에, 수경재배 딸기는 베드 하단에, 수경재배 토마토는 베드 위 20 cm에 설치하였던 끈끈이트랩에서 버섯파리류 유인수가 가장 많았다. 본 연구는 버섯파리류 성충의 효율적인 예찰 및 방제를 위해서 작물의 재배형태나 환경에 따라 끈끈이트랩의 설치 위치를 달리하여야 되는 것을 시사했다.

Neodymium-iron-boron (Nd-Fe-B) sintered magnets have excellent magnetic properties such as the remanence, coercive force, and the maximum energy product compared to other hard magnetic materials. The coercive force of Nd-Fe-B sintered magnets is improved by the addition of heavy rare earth elements such as dysprosium and terbium instead of neodymium. Then, the magnetocrystalline anisotropy of Nd-Fe-B sintered magnets increases. However, additional elements have increased the production cost of Nd-Fe-B sintered magnets. Hence, a study on the control of the microstructure of Nd-Fe-B magnets is being conducted. As the coercive force of magnets improves, the grain size of the Nd2Fe14B grain is close to 300 nm because they are nucleation-type magnets. In this study, fine particles of Nd-Fe-B are prepared with various grinding energies in the pulverization process used for preparing sintered magnets, and the microstructure and magnetic properties of the magnets are investigated.

In order to evaluate the role of macrophytes as refuge of zooplankton on physical distribution (i.e. summer rainfall), we investigated the environmental factors, macrophytes, and zooplankton in waterside zones (macrophytes zones) and open water zones of 17 wetlands from May and August, 2011. In this study, a total of 51 zooplankton species were identified, and Polyarthra sp. and Diaphanosoma brachyurum were found to be the most dominant species. Waterside area of each wetland were occupied by a total of 10 macrophyte species, species composition and biomass (dry weight) were different in the survey sites. Zooplankton was more abundant in waterside zone than open water zones lacking macrophytes (One-way ANOVA, df=2, F=27.1, P<0.05), in particular, waterside zone of 1, 8, 9, 10, and 11 wetland were supported by high zooplankton density after summer rainfall. This wetlands were developed by various macrophyte species than other wetland, and submerged plant commonly presented. Waterside zones with various macrophyte species provides complexity to the habitat structure, should be utilized as refuge to avoid disturbance such as summer rainfall. The results indicate that macrophytes are the key components to enhance bio-diversity include zooplankton, and the inclusion of diverse plant species in wetland construction or restoration schemes will result in ecologically healthy food webs.

Ribosomal protein L21 (RPL21) plays an important role in ribosome assembly. It is considered to be a major cause for the occurrence of the hypotrichosis simplex (HTS), a type of sustained hair loss from early childhood to adulthood. In this study, the full-length sequence of pig RPL21 gene (GenBank accession number: KU891824) was cloned and identified for the first time. We found it contains a 483-bp open reading frame (ORF) encoding 160 amino acids. It is located in the plus strand of chromosome 11, which spans 2,167 bp from 4,199,792 to 4,201,958. We found RPL21 expression level is closely related to cell proliferation and cell cycle arrest. In the knockdown group, the cell proliferation activity was significantly decreased (P<0.01) and an obvious accumulation of cells at the G2/M phase with a simultaneous up-regulation of p53 and p21 was observed. This likely due to knockdown of RPL21 triggered ribosomal stress, which affected the normal ribosome assembly and caused defective ribosome biogenesis. The unassembled RPs were released consequently from the nucleolus to the nucleoplasm where they can activate p53-dependent cell-cycle responsive factors and led to a G2/M arrest. We expect these results may provide valid information for further study on the pig RPL21 gene and the cause of hypo trichosis simplex.

Armor block is used to reduce wave energy. Concrete armor block is prone to be damaged under the ultimate load like typhoon. It is necessary to develop a new type of armor block to enhance to the strength. So, the research of new type armor block..

In order to research the wave characteristics in Suyeong bay on typhoon in 2011, this study was carried out observation of wave on typhoon "Ma-on",calculation of wave characteristics using STWAVE and comparison with significant wave heights between observ

Damages of medicinal crops by thrips and identification of thrips species were investigated on major medicinal crops, Platycodon grandiflorum, Schizandra chinensis, Codonopsis lanceolata, Lycium chinense, Bupleurum falcatum, Ledebouriella seseloides, Angelica gigas, Glycyrrhiza uralensis, Liriope platyphylla, Atractyloides sp., in Suwon, Eumseong, Jecheon, Pyeongchang, Chyungyang 2012. Thrips and their damages were found at all of the medicinal crops investigated, mostly occurring at early growing stages of each medicinal crop and causing apical meristem region withered, thereby inhibiting the growth of plants. As a result, Frankliniella intonsa, Thrips tabaci, and Megalurothrips distalis have been identified as dominant species on medicinal crops in open field, whereas Frankliniella occidentalis was restricted to Lycium chinense in which protected cultivation. Occurrence of thrips species on medicinal crops seems to be affected by host specificity and environmental factors such as cultivation types.

Intermediate disturbance hypothesis is one of important hypotheses for presenting the diversity patterns under disturbances. To investigate the diversity patterns of benthic macroinvertebrate communities under various disturbances, we collected macroinvertebrates in 47 sites representing various habitat types as well as pollution gradients in Southeastern Korea in summer and winter. Environmental variables such as depth, velocity, substrate and conductivity, and biological water quality indices (e.g., BWMP) were measured to show the intensity of disturbance in each site. Species abundance distributions (SADs) which are one of the efficient ways to assess community structure states based on macroecology were also considered to represent the pollution status based on the community patterns along an established pollution gradient. The community patterns were broadly divided into log-normal distribution and geometric series groups according to SAD models with weak and strong disturbance, respectively. According to the application of physicochemical water quality variable (conductivity), the gradient of community indices showed higher values on the intermediate disturbance state. Higher values of community indices, however, were presented on the low disturbance state with the gradient from BMWP. The parameters of SAD models, log-normal distribution, γ, and geometric series, k, were additionally calculated and compared with the traditional community indices.