본 연구는 소양강댐 하류에서 서식하는 생태계교란 생물 종인 브라운송어와 그 먹이원으로 이용되는 저서성 대형무척추동물에 대한 파악을 위해 2022년부터 2023년까지 총 8회에 걸쳐 소양강댐 하류(St.1~St.3)와 지류 (St.4)에 대해 브라운송어와 공서종, 브라운송어의 위 내용물, 저서성 대형무척추동물의 종조성 및 기능군 분석을 실시하였다. 저서성 대형무척추동물의 경우, 하루살이목에서 가장 많은 분류군이 확인되었으며(27.1%), 그 중 붙는 무리(CL)와 헤엄치는 무리(SW)가 높은 비율을 차지하는 것으로 확인되었다. 브라운송어 채집 결과, 전장은 26∼246mm까지 총 105개체가 채집되었으며, 전장-체중 관계의 매개변수 b값이 3을 초과하여 안정적인 성장이 이루어지는 것으로 확인되었다. 브라운송어의 위 내용물에 대한 먹이원 분석 결과, 빙어(0.2%, TL: 246mm)와 육상곤충(2.7%, TL: 154mm, 183∼185mm)을 섭식한 개체는 매우 적었으며 상대적으로 전장이 큰 개체에서 확인 되었다. 대부분 수서곤충(73.8%)과 물 속에서 서식하는 비곤충류(23.3%)를 섭식하는 것으로 나타났다. 브라운 송어의 전장에 따른 먹이 섭식 패턴을 파악하기 위해 위 내용물에서 확인된 종들과의 상관분석을 실시한 결과, 브라운송어의 먹이원 중 유수성 환경 선호 종들의 경우 전장과 양의 상관관계(p<0.05)를 나타낸 반면, 모래 기질 이하의 흐름이 적은 서식처를 선호하는 종들의 경우 전장과 음의 상관관계(p<0.05)를 나타냈다.

The purpose of this paper was to investigate the comparison of balance and muscle strength between dominant and non-dominant legs in adults. Thirty adults in their 20s participated in this study. The dominant and non-dominant legs were selected based on the dominant hands of the target. The subject's muscle strength of legs was measured with Nicholas MMT, and the balance was measured with BIO-Rescue. We compared the dominant and non-dominant legs based on the results. The result, indicated no statistical difference on balance and muscle strength between dominant and non-dominant legs(p>.05). The results of this study will be helpful in setting the effective treatment direction and treatment level, and in controlling posture, balance and motor function.

Incursions of red imported fire ant (RIFA), Solenopsis invicta into Korea have been increasing. After a first interception of a colony of S. invicta on Gamman pier, Pusan port while intensive surveillance by Animal & Plant Quarantine Agency (APQA) in September 2017, three more RIFA colonies have been found in sea port piers of Pyeongtek, Incheon and Pusan cities. The social forms and mitochondrial DNA haplotypes of the intercepted RIFA colonies were analysed by allelic discrimination assay (peptide nucleic acid probe based RT-PCR) of Gp-9 gene and mt-DNA fragment of 768 bp, which is part of the Cytochrome oxidaseⅠ gene. The colony on Gamman pier, intercepted in Sep. 2017 was previously reported as a haplotype 5 (H5) of mitochondrial DNA and a social form of polygyne. The colony on Hutchison pier of Pusan port, intercepted in June 2018 were confirmed as a H22 haplotype and a monogyne. Those different social forms show different origins of each colonies. Those on piers of Pyeongtek and Incheon ports, also found in 2018 were confirmed samely as H22 and monogyne. However, it could be putatively assumed that those two colonies were differently introduced via different container cargoes, considering those colonies were found in container yards of distantly located different sea ports. More genetic variation analyses using diverse sets of molecular markers such as microsatellites, genome-wide single nucleotide polymorphisms, etc. in nuclear gens are being proceeded for more exact introduction routes (origins).

벼메뚜기는 분류학적으로 메뚜기목(Orthoptera), 메뚜기과(Acrididae)에 속하는 곤충으로 작물에 피해를 주는 해충으로 인식하고 있지만 예로부터 단백질 보충을 위하여 채집하여 튀기거나 볶음요리 로 이용해 왔다. 벼메뚜기는 갈색거저리, 쌍별귀뚜라미 등과 함께 식품공전에 등록되어 있으나 1년 1세대로 가을철에 채집하여 이용하는 실정으로 공급확대에 한계가 있다. 최근 우리나라에서 벼메뚜기를 사육하고자 하는 농가와 다양한 용도로 가공을 통해 이용하려는 수요가 점차 증가하고 있으나 연중사육기술이 개발되지 않아 대량사육하는 농가가 거의 없는 실정이다. 이에 벼메뚜기의 연중대량사육기술 뿐만 아니라 인공사료 및 자동화 사육시설 개발에 따른 생산비 절감 기술 개발이 필요한 실정이다. 벼메뚜기의 먹이로는 여름작물로 옥수수, 수단그래스, 겨울작물로 밀, 보리 등의 볏과작물이 있으며 많은 양의 생엽과 발육에 적합한 작물의 선발 및 볏과작물의 통곡실 가루를 이용한 인공사료의 개발로 먹이공급 부족 시 15~20일 급여 가능하며, 벼메뚜기 사육시설로 단독형, 연결형, 절충형 등의 사육시설을 개발하여 연결형 사육시설의 수확량과 생존율이 가장 높았으며, 예취 급이 노동력이 단독형의 16% 소요되었다.

Generally, fate of spematogonial stem cells (SSCs) can be determined specifically by microenvironments enclosed with various extracellular matrix (ECM) components and integrins recognizing directly ECM proteins play an pivotal role in transporting ECM-derived signals into cytoplasm, resulting in inducing a variety of biological functions such as cell attachment, self-renewal and differentiation. However, to date, studies on type of integrins expressed on the undifferentiated SSCs remain unclear. Therefore, we tried to investigate systematically what kind of integrin subunits are expressed transcriptionally or translationally in the SSCs derived from testis of hybrid B6CBAF1 mouse. For these, isolation of SSCs from testis were conducted by magnetic activated cell sorting (MACS) using Thy1 antibody. Subsequently, transcriptional and translational level of integrin α and β subunits in the isolated SSCs were measured by real-time PCR and fluorescene immunoassay, respectively. As the results, transcriptional levels of genes encoding total 25 integrin subunits were quantified, and integrin α4, α6, α7, α9, αV, αL and αE and integrin β1, β5 showed higher expression levels than other subunits. By contrast, integrin α3, α5, α 10 and α11 and integrin β2, β3, β4, β7 were weakly transcribed. When translational levels of the integrin α subunits showing high transcription level (α4, α6, α7, α9, αV αL, and αE) were measured, integrin α6, α7, α9, αV and αL were higher than integrin α4 and αE. In case of integrin β subunit, β1 evaluated more expression than β5. From these results, we speculate that the undifferentiated SSCs derived from hybrid B6CBAF1 mouse may express integrin α4β1, α6β1, α7β1, α9β1, αVβ1 and/or αVβ5 on plasma membrane. Moreover, this information will greatly contribute to constructing non-cellular niche supporting self-renewal of SSCs in the future.

The oedemerid species Oedemera sexualis Marseul, 1877 is reported for the first time from Korea. The genus Oedemera Olivier, 1789, within subfamily Oedemerinae and family Oedemeridae, is one of the common oedemerid beetles being widely distributed throughout the world. The world-widespread genus Oedemera is presented 3 subgenera and 104 species in Palaearctic region. Until now, 8 species and subspecies were recorded in Korea. O. sexualis was found in Is. Yongcho and Is. Bijin in Tongyeong-si, Gyeongsangnamdo of Korea, bringing the number of Korean Oedemera species to 9 species. In this study, we report the taxonomic key to the genus Oedemera of Korea, and morphological description of O. sexualis.

Nitidulid beetles of the genus Aethina Erichson, 1843 are a well-known group as harmful to agricultural and apicultural industry, especially including A. tumida, an American small hive beetle, which is a beekeeping pest. The world-widespread genus Aethina, within subfamily Nitidulinae and family Nitidulidae, is presented 5 subgenera and 28 species in Palaearctic region. Until now, 2 subgenera, Aethina s. str. and Aethina (Circopes), and 3 species, A. (A.) flavicollis, A. (A.) inconspicua and A. (C.) suturallis, have been recorded in Korea, and we added one more species, A. (A.) maculicollis as a newly recorded species. The larvae of A. (A.) maculicollis have been reported as pests of mulberry fruits in Japan. In this study, we report the taxonomic key to the genus Aethina of Korea, and morphological description of A. (A.) maculicollis.

Olibrus Erichson, 1845 and Olibrus particeps Mulsant & Rey, 1861 (Coleoptera: Cucujoidea: Phalacridae) are reported from Korea for the first time and historical review of the taxonomic position of this genus is provided. The genus Olibrus Erichson is one of the common phalacrid beetles being widely distributed throughout the world. This genus is easily distinguished from other phalacrid genera by combination of the following characters: Antennae inserted at sides of front, base visible from above; Last segment of antenna softly indented; Basal metatarsomere shorter than second; Elytral surface very polished. O. particeps was found in Andong-si and Yeongju-si, Gyeongsangbuk-do of Korea, bringing the number of species within the Korean Phalacridae to 2 species. In this study, we provide a redescription of O. particeps Mulsant & Rey, and illustrations of its genitals and other appendages.

The present study investigated the effects of follicle stimulating hormone (FSH) and human chorionic gonadotrophin (hCG) on the nuclear maturation of canine oocytes. Oocytes were recovered from mongrel female ovaries in various reproductive states; follicular, luteal or anestrous stage. Oocytes were cultured in serum-free tissue culture medium (TCM)-199 supplemented with various concentrations of FSH (Exp. 1: 0, 0.5, 1.0 or 10 IU) or hCG (Exp. 2: 0, 0.5, 1.0 or 10 IU) or both (Exp. 3: 1 IU FSH + 1 IU hCG) for 72 hr to determine the effective concentration of these hormones, and to examine their combined effect. After maturation culture, oocytes were denuded in PBS containing 0.1% (w/v) hyaluronidase by gentle pipetting. The denuded oocytes were stained with 1.9 μM. Hoechst 33342 in glycerol and the nuclear state of oocytes was evaluated under UV light. More (p<0.05) oocytes matured to MII stage when follicular stage oocytes were supplemented with 1 IU FSH (6.2%) compared with the control, 0.1 or 10.0 IU FSH (0 to 1.2%). Significantly higher (p<0.05) maturation rate to MII stage was observed in follicular stage oocytes supplemented with 1.0 IU hCG (7.2%) compared with the control or other hCG supplemented groups (0 to 1.5%). However, the combination of FSH and hCG did not improve the nuclear maturation rate of canine oocyte (2.4 %) compared with FSH (6.2%) and hCG alone (7.2%). In conclusion, FSH or hCG alone significantly increased the maturation of canine oocytes to MII stage.

고무화합물 형태로 구성된 조영제의 병에 Syringe Connector의 Spike를 연결 시 고무의 찢김 정도를 알아보고 찢김 및 분쇄로 인한 합성고무의 혼입 유무와 분쇄된 합성고무가 검출 시 분쇄물의 크기를 실험을 통해 알아보고자 하였다. 그 결과 찢김 정도의 경우 Syringe Connector의 끝과 최초 접촉하는 앞면이 약 3.14±0.04 ㎜로 뒷면 보다 많이 찢겼으며, 실험 대상인 10 병의 조영제에서 평균 7 개에서 15 개로 모두 분쇄물이 검출되었다. 검출된 분쇄물을 이용하여 크기를 측정한 결과 평균크기는 약 7.89±0.31 ㎛이었다. 향 후 다양한 실험 및 분석방법을 통한 추가실험과 더불어 흡인된 분쇄물 차단을 위한 미세 필터타입 자동주입장치의 개발이 필요하며, 분쇄물 유입 시 치명적 사고를 대비하여 관련기관의 관심 또한 필요할 것으로 사료된다.

As a result of inquiring into the analysis consequent on a symptom by research subject, it was found that there were 1 paper (1.5%) which did research for the purpose of rehabilitation of the general public, 26 papers (38.8%) targeting diagnosis-related groups (DRG), and 40 papers (59.7%) targeting social consideration subject. Also, as a result of the inquiry about the frequency & number of times of horticultural therapy program implementation, it was found that 49 papers (73.1%) implemented one time a week, and 45 papers (67.2%) were surveyed as the highest by conducting a total of 11~20 sessions of horticultural therapy programs, In the analysis of horticultural therapy activities by type, plant cultivation activity was found to be the most 506 times, accounting for 41.5%, followed by 297 time crafts activity (24.4%), 213 time floral decoration activity (17.5%), and 203time others activities (16.7%). In cultivation activity, soil-using cultivation activity (25.1%) was found to be lower than the proportion (74.9%) of soiless cultivation (16.4%), crafts activity (24.4%), floral decoration activity (17.5%) and other activities (16.7%). The most used plants in a restricted place like a hospital were found to be in the order of Hedera helix, Chamaedorea elegans, Succulent plant, Syngonium podophyllum, Neofinetia falcata HU, Hoya carnosa (L.f.) R.Br.), Rosmarinus officinalis, and Spathiphyllum spp.

Background : Five medicinal herbs have been selected from the preliminary screening for in vitro anti-allergy activity (in RBL-2H3 cells). The present study is conducted to investigate the inhibitory effects of the medicinal herbs on allergic inflammation in other kind of cells. Methods and Results : Cells treated with five extracts prepared from Betula costata Trautv. (aerial part), Camellia sinensis L. (aerial part), Polygonatum stenophyllum Maxim. (root), Pyrus pyrifolia var. culta (Makino) Nakai (leaf), and Rehmannia glutinosa (Gaertn.) Libosch. ex Steud. (root) were measured for mRNA levels of TNF-α on HaCaT keratinocytes stimulated by TNF-α /INF-γ and for mRNA levels of IL-2 in Jurkat T cells mediated by PMA/A23187. Pre-treatment with the five extracts reduced the mRNA levels of TNF-α in HaCaT cells and mRNA levels of IL-2 in Jurkat T cells. In particular, the leaf extract of Pyrus pyrifolia var. culta (Makino) Nakai significantly and dose-dependently decreased the mRNA levels of TNF-α and IL-2. To determine the toxicity of the extracts from the selected medicinal herbs to HaCaT cells and Jurkat T cells, the viabilities of the cells treated with several concentrations of the five extracts were measured by MTT assay. Extracts of Polygonatum stenophyllum Maxim. (root), Pyrus pyrifolia var. culta (Makino) Nakai (leaf), and Rehmannia glutinosa (Gaertn.) Libosch. ex Steud. (root) (up to 250 ㎍/㎖) did not show cytotoxic effects on HaCaT cells and Jurkat T cells. On the other hand, 250 ㎍/㎖ of extracts of Betula costata Trautv. (aerial part) and Camellia sinensis L. (aerial part) reduced cell viability in both cells. Conclusion : These results demonstrate that the leaf extract of Pyrus pyrifolia var. culta (Makino) Nakai has an anti-inflammatory effect by inhibiting pro-inflammatory cytokine expression. Therefore, the leaf of Pyrus pyrifolia var. culta (Makino) Nakai can be a useful resource for the development of anti-allergy/anti-inflammatory materials.