Tuberculosis is a potentially deadly infectious disease caused by the Mycobacterium tuberculosis (M. tuberculosis). Tuberculosis is diagnosed by proving the M. tuberculosis in sputum samples based on the results of acid-resistant staining, culture, and nucleic acid amplification tests. However, there is a report that the detection rate of M. tuberculosis is low in acid-resistant staining using tissue specimens. It has been suspected that the cause is a potential loss of acid resistance by the organic solvents used for tissue specimen preparation. Therefore, this study was pursued to find out if Gram staining and fluorescent staining in addition to acid-resistant staining would be helpful in diagnosing tuberculosis. We used four tissue (lung, small intestine, large intestine, and lymph node) samples with chronic granulomatous inflammation observed in HE staining and positive results in real-time PCR. These detection rates and staining properties were investigated through microscopic examination using the Ziehl-Neelsen, Gram, and Auramin rhodamine staining. In this studies, M. tuberculosis were observed by Ziehl-Neelsen, Gram, and Auramin rhodamine staining in all four samples. In the evaluation of clinical microbiology proficiency testing (CMPT), the Ziehl-Neelsen and Gram staining were the same result, but the Auramin rhodamine staining was relatively low. These data indicated that Gram staining is useful for detecting M. tuberculosis in formalin-fixed tissue specimens. Therefore, if the Ziehl-Neelsen and Gram staining are combined as the M. tuberculosis staining method in tissue specimens, a better direction may be provided for tuberculosis diagnosis.

수십 년간의 세계화에도 불구하고, 기업들은 여전히 국내와 해외 시장에서의 마케팅 환경의 불일 치로 어려움을 겪고 있다. 기업들은 이러한 환경의 차이를 극복하기 위해 각 시장의 특성을 고려한 광고 어필(advertising appeal)을 이용하여 특정 메시지를 전달한다. 이에 따라 기업의 국내와 해외 시장에서의 광고 어필 사용의 차이가 존재하는데, 그 차이를 찾기 위해 본 연구는 한국과 말 레이시아에서 한국브랜드가 어떤 광고 어필을 활용하는지 살펴보려고 한다. 이번 연구는 유튜브 동 영상의 콘텐츠 분석을 통해 한국과 말레이시아 시장 간 광고 어필 사용의 차이를 도출하였다. 본 논문은 말레이시아 시장에서의 마케팅 커뮤니케이션 전략을 계획할 때 국내 마케팅 매니저들이 고 려할 수 있는 실질적인 시사점을 제시한다.

토마토 온실에서 담배가루이 (Bemisia tabaci) 유인을 위한 작물에 따른 선호도를 조사하였다. 피망, 토마토, 오이, 가지의 4 종류의 작물을 아크릴케이지에 같이 넣어두고 담배가루이 성충 30쌍을 방사하여 선호도 및 산란수를 조사하였다. 담배가루이 성충은 오이(약 40%)와 가지(약 40%)에서 가장 선호하였으며, 토마토(10%)와 피망(10%)의 선호도는 낮았다. 기주에 따른 담배가루이의 성충 30쌍의 산란수는 오이에서 485개로 가장 높았으며 가지(250), 토마토(131), 피망(126)의 순으로 조사되었다. 토마토 온실에 가지묘를 정식하였을 경우에는 토마토에서는 주당 약 20마리의 담배가루이가 조사되었으나 가지에서는 주당 400∼500마리의 담배가루이가 조사되어 토마토 온실에서 가지를 이용한 담배가루이 유인이 가능함을 알 수 있었다.

본 연구에서는 주요 발생지인 구례 지역의 산수유, 때죽나무를 중심으로 2018년 8월에서 9월 사이에 갈색날개매미충 월동 난괴를 채집하여 날개매미충알벌(phanuromyia spp.)의 온도별(15℃, 20℃, 25℃, 30℃) 발육기간, 수명 및 우화 후 15일간의 총 산란수를 조사하였다. 날개매미충알벌의 발육기간은 25℃에서 알 2.6일, 유충 5.4일, 번데기 15.0일, 30℃에서 알 1.9일, 유충 7.6일, 번데기 13.7일이 소요되었다. 날개매미충알벌 성충의 온도별 수명은 15℃에서 암컷 77.4일, 수컷 66.6일, 20℃에서 암컷 49.0일, 수컷 44.0일, 25℃에서 암컷 27.6일 수컷 28.4일, 30℃에서 암컷 18.0일, 수컷 14.0일로 암수 모두 사육온도가 높아질수록 수명이 짧아졌으며 15℃와 30℃에서만 암수 수명의 차이가 있었다. 또한 온도별 총 산란 수는 15℃를 제외한 나머지 세 가지 사육온도에서 산란 수 차이는 없었으나 25℃에서 평균 23.2개로 가장 높았다.

It was available to store both nymphs and adults of Geocoris pallidipennis at 5℃ for 3 weeks without negative effects on their survival, fecundity, and Bamisia tabaci nymph predation. Up to 140 days, it was also possible to store Micromus angulatus adults at low temperature, showing approximately 50% cumulative survival rate, but over 2 week cold storage was not suitable for their fecundity. With the increase of cold storage duration, the aphid predation by M. angulatus decreased, but it could be possible to cold store until 5 weeks without a significant effect on their aphid predation. Thus, short term cold storage of G. pallidipennis and M. angulatus could be useful for inundative biological control.

약제 처리에 따른 담배거세미나방(Spodoptera litura) 유충의 섭식 행동 및 반응을 조사하기 위해 1분 간격으로 잎면적을 자동 측정하였다. 담배거세미나방 유충의 섭식 행동은 섭식기와 비섭식기가 뚜렷하게 구분되었으며, 섭식기가 끝나면 비섭식기를 거쳐 탈피를 하고 다시 섭식기에 들어갔다. 1회 섭식 지속 시간은 약 15분으로 조사되었고, 영기 내 평균 섭식 회수는 약 27.5회로 섭식 지속 시간과 마찬가지로 영기에 따른 차이는 없었다. 약제 처리는 피망 잎을 Buprofezin methoxyfenozyd과 BT (Bacillus thuringiensis) 500ppm 약액에 10초간 침지 처리하였다. Buprofezin methoxyfenozyd를 처리 하였을 경우 섭식 시간은 평균 8~9분으로 감소하였고, 치사 전까지 섭식량은 각각 176mg으로 조사되었다. 하지만 BT제를 처리 시에는 담배거세미나방 유충은 치사하지 않았다.

Probiotics, enzymes, organic acids, oligosaccharides, antioxidants, and other functional materials are actively being explored as alternatives to antibiotics. Probiotics include live beneficial microorganisms that colonize the intestinal tract and competitively inhibit attachment and growth of harmful microbes. Probiotics also increase feed efficiency by assisting in nutrient absorption and digestion. The current study was conducted in order to evaluate the effect of a new probiotic, CS-A, as a dietary supplement of a fermented product on growth performance, feed intake, and feed conversion efficiency in broiler chickens, and to evaluate its value as an alternative for antibiotics used as a feed additive. Antibacterial and anti-inflammatory effects of CS-A were investigated in vitro and the in vivo effects of a constant concentration of supplemented CS-A on growth rate and feed efficiency were evaluated. In addition, the safety of CS-A was assessed by examination of common symptoms and mortality. Determination of minimal inhibitory concentration revealed an excellent antibacterial effect of CS-A. Cytotoxicity was low and anti-inflammatory effects were achieved at the effective concentration of CS-A. Supplementation with 0.1% CS-A resulted in a feed efficiency score of 1.84 in broilers, compared to 2.00 in the control group. There were no adverse clinical findings, necropsy findings, hematology, and altered serum biochemistry parameters, and no mortality. Thus, it is concluded that CS-A is safe and effective as a feed additive.

The mannanase, cellulase and xylanase-producing thermophilic bacteria, designated EJ3, was isolated from fresh spent mushroom (Pleurotus eryngii) substrates taken from the DOJUN farm located in Keyongnam, Korea. The isolate EJ3 was facultatively anaerobic and grew at temperature ranging from 20℃ to 60℃ with an optimal temperature of 37℃. The DNA G+C content of the isolate EJ3 was 45 mlo%. The major fatty acids were anteiso-15:0 (38.9%), 17:0 (7.4%), and iso-15:0 (38.2%). The 16S rRNA gene sequence similarity between the isolate EJ3 and other Bacillus strains varied from 97% to 99%. In the phylogenetic analysis based on these sequences, the isolate EJ3 and Bacillus subtilis clustered within a group and separated from other species of Bacillus. Based on the physiological and molecular properties, the isolate EJ3 was classified within the genus Bacillus as Bacillus subtilis EJ3. The optimal growth condition of B. subtilis EJ3 were pH 6.0 and 55℃, respectively.

This study aimed to produce fermented soy-powder milk (FSPM) with Lactobacillus plantarum P1201 and to evaluate its anti-obesity activity. Isoflavone and conjugated linoleic acid (CLA) of unfermented soy-powder milk (UFSPM) and FSPM and were analyzed via high-pressure liquid chromatography (HPLC) and gas chromatography (GC). Their inhibitory activities against α-glucosidase, α-amylase, and pancreatic lipase were assayed. Their anti-obesity activities were evaluated on the basis of their inhibitory effects on adipocyte differentiation in 3T3-L1 cells, and the expression of mRNAs associated with adipogenesis and lipid metabolism were analyzed via real time-polymerase chain reaction (RT-PCR) and quantitative PCR (qPCR). FSPM with L. plantarum P1201 increased the isoflavone aglycones (daidzein, glycitein, and genistein) content and produced CLA in soy-powder milk (SPM), both of which possessed bio-activity. Both UFSPM and FSPM showed dose-dependent inhibitory activity for α-glucosidase, α-amylase, and pancreatic lipase. FSPM, but not UFSPM, suppressed adipogenesis in 3T3-L1 cells and reduced their triglyceride content by 23.1% after treatment with 1,000 μg/mL of FSPM, compared with the control group. The anti-obesity effect of FSPM can be attributed to CLA and isoflavone aglycones, which targeted CCAAT/enhancer binding protein α (C/EBP-α) and down-regulated lipoprotein lipase (LPL), adiponectin, adipocyte fatty acid-binding protein (aP2), fatty acid synthase (FAS), and acetyl CoA carboxylase (ACC) mRNA. Furthermore, FSPM enhanced the inhibitory activity of glucosidase and pancreatic enzymes and anti-obesity activity. Further studies are required to investigate whether the anti-obesity effect of FSPM persists in an in vivo mouse model of diet-induced obesity.

In this study, soy-powder yogurt (SPY) with enhanced levels of γ-aminobutyric acid (GABA) and isoflavone aglycone was produced from sprouting high-protein soybeans (HPSs). The fermented steam-HPS sprouts (0 to 4 cm) were fermented (72 h) with Lactobacillus brevis, and the total free amino acids (FAAs) of the formed mixtures were determined to be 79.53, 489.93, 877.55, 780.53, and 979.97 mg/100 mL in the fermented HPS (FHPS), and the fermented steam-HPS with 0 cm (FSHPS-0), 1 cm (FSHPS-1), 2 cm (FSHPS-2), and 4 cm sprouting lengths (FSHPS-4), respectively. The levels of glutamic acid (GA) and GABA were observed to be the highest, 100.31 and 101.60 mg/100 mL, respectively, in the unfermented HPS (UFSHPS-1, 1 cm) and FSHPS-1 sprouts, respectively. Moreover, the total contents of the isoflavone glycoside form decreased proportionally to the increasing total levels of isoflavone aglycones after fermentation in FSHPS-0, FSHPS-1, FSHPS-2, and FSHPS-4. The levels of isoflavone aglycones were detected as 350.34, 289.15, 361.61, 445.05, and 491.25 μg/g in FHPS, FSHPS-0, FSHPS-1, FSHPS-2, and FSHPS-4, respectively. While FSHPS-1 exhibited the highest DPPH (63.28%) and ABTS (73.28%) radical scavenging activities, FSHPS-4 contained the highest isoflavone aglycone ratio (81.63%). All in all, the FSHPS-1 mixture prepared in this study exhibited high GABA content and functional prosperity, thereby making it suitable for potential applications in the soy-dairy industry.

A resveratrol synthase (RS) gene was isolated from peanut (Arachis hypogaea, L. cv. Jinpoong) plants. This gene was placed under the control of the cauliflower mosaic virus 35S promoter (CaMV35S) and introduced into two Korean varieties of potato (Solanum tuberosum L. cvs. Jasim and Jowon) plants by Agrobacterium-mediated gene transfer. Putative transformants were screened by PCR with primers designed from CaMV 35S promoter, NOS terminator and RS gene. Most of selected transgenic potato plants showed the amplification of expected fragments by PCR of genomic DNA with gene-specific primers, while they were absent in untransformed control plants. Expression of the resveratrol synthase gene was also examined by northern blot analysis. The transformants showed a band which was lacking in the control plant, confirming that the introduced gene is transcribed into mRNA in the transformants. The strength of the band, which reflected the level of mRNA expression, differed among the individual transformants. Among the transformants obtained, the highest trans-resveratrol content in the transgenic young leaves of purple-fleshed "Jashim" was 2.11 μgg-1 fresh weight and that in the microtubers in vitro of purple fleshed "Jashim" was 8.31 μgg-1 fresh weight. This amount of resveratrol may have a positive biological effect on human health.