The purpose of this study was to analyze lower limb muscle activity and 3D motion analysis according to change foot arch height during walking. We selected 9 young and healthy people who have been normal foot. And we selected 7 young and healthy people who have been flatfoot. So, people were divided into 2 groups and walked platform during 2 minutes twice for checked by 3D motion analysis. These data were characterized by EMG measurements of three muscles( tibialis anterior, medial and lateral gastrocnemius) while they were walking. The collected data were analyzed by Independent t test using the SPSS statistics program(Ver 12.0). In foot arch change, there were no significant difference in three muscles 3D motion analysis also found that there were no significant difference in joint angles. In this study was to analyze lower limb muscle activity and 3D motion analysis according to change foot arch, but there were no significant difference in 6 muscles neither joint angles.
We have generated transgenic mice that expressed mouse extracellular superoxide dismutase (EC-SOD) in their skin. In particular, the expression plasmid DNA containing human keratin K14 promoter was used to direct the keratinocyte-specific transcription of the transgene. To compare intron-dependent and intron-independent gene expression, we constructed two vectors. The vector B, which contains the rabbit -globin intron 2, was not effective for mouse EC-SOD overexpression. The EC-SOD transcript was detected in the skin, as determined by Northern blot analysis. Furthermore, EC-SOD protein was detected in the skin tissue, as demonstrated by Western blot analysis. To evaluate the expression levels of EC-SOD in various tissues, we purified EC-SOD from the skin, lungs, brain, kidneys, livers, and spleen of transgenic mice and measured its activities. EC-SOD activities in the transgenic mice skin were approximately 7 fold higher than in wild-type mice. These results suggest that the mouse overexpressing vector not only induces keratinocyte-specific expression of EC-SOD, but also expresses successfully functional EC-SOD. Thus, these transgenic mice appeared to be useful for the expression of the EC-SOD gene and subsequent analysis of various skin changes, such as erythema, inflamation, photoaging, and skin tumors.
"Gopumbyeo" is a new japonica rice variety developed and registered by the rice breeding team of National Institute of Crop Science, RDA in 2004, which was derived from a cross between a line SR15225-B-22-1-2 which has good eating-quality and high yield potential and a line SR15140-58-2-2-3 which has lodging and disease resistances. This variety has medium heading date of Aug. 13, medium culm length of 78 cm and tolerance to lodging. It has also relatively semi-erect pubescent leaf blades and slightly tough culm with good canopy architecture. Gopumbyeo has a bit less number of tillers per hill and more spikelets per panicle than Hwaseongbyeo. It shows less sensitivity to premature heading in delayed transplanting of 50-day old seedlings. It showed longer delay of heading date but higher spikelet fertility than Hwaseongbyeo when exposed to cold stress. This variety shows delayed leaf senescence and considerable tolerance to viviparous germination during ripening. It shows moderate resistance to leaf blast, and bacterial blight, but susceptibility to stripe virus and insect pests. The milled rice of Gopumbyeo exhibits translucent, clear non-glutinous endosperm and medium short grains. It shows similar amylose content of 19.6%, and gelatinization temperature, and better palatability of cooked rice compared to Hwaseongbyeo. The milled rice yield of this variety is about 5.48 MT/ha at ordinary culture in local adaptability test for three years. Gopumbyeo would be highly adaptable to the central lowland and mid-southern mountainous areas of Korea.
The pollen grain is a unique tricellular structure suitable for the delivery of the sperm cells to the ovule. All nutrients required for microspore and pollen cell growth are derived by passage through the anther locule and secretion by the tapetum lining. During later stages the tapetum degenerates but contributes to produce pigments, waxes, lipids and proteins which form the pollen coat and function in signaling between male (pollen) and female (pistil) tissues. The development of both normal pollen and tapetum is necessary for the fertilization processes in rice and would be exploited for the induction of male-sterility which is very useful to improve economic value of crops.
We aredeveloping new approaches using a conditional male-sterility for the F1 hybrid seed production in rice. The conventional three parental systems for F1 hybrid seed production requirethe following three lines: male-sterile line, maintainer line, and restorer line. In this system, a critical requirement is to maintain the male-sterile inbred lines. Here we suggest molecular approaches, in which the engineered male-sterile plants are generated by regulating endogenous hormonal balance through the loss-of-function of genes. We can expect the male-sterility can be restored by exogenous applications of hormones such as gibberellin or jasmonic acid. Based on two parental systems, we will address the answer onfollowing question: how can we maintain a male-sterile line producing 100% male-sterile progenies without a maintainer?
This work was supported by grants from Crop Functional Genomics Center of the 21C Frontier Program (CG1517), RepublicKorea.
“Jinpumbyeo” is a new japonica rice cultivar developed from a cross between lodging tolerant semi-dwarf plant type, SR14703-60-5-GH1 and high eating quality cultivar Suweon353 by the rice breeding team of National Crop Experiment Station, RDA made in 1987