of the marketed multi-later insulating curtain was carried out. Experiments is conducted by fabricating a test apparatus for investigating the heat flux characteristics. The multi-later insulating curtain used for the experiment was compared using the P, N, S, U and T company, which are commercially available, and the heat flux due to temperature difference between the experimental apparatus and the outside was compared and analyzed. When the internal temperature of the experimental result is the maximum temperature 60℃, the heat flux of multi-later insulating curtain is T Co.(73.1W/m2) > S Co.(119.5W/m2) > U Co.(155W/m2) > N Co.(163.1 W/m2) > P Co.(177.7W/m2). The heat flux means the quantity of heat passing through the unit time per unit area, and the higher the numerical value, the higher the quantity of heat passing through the multi-layer insulating curtain. This can be determined that high heat fluxes produce low heat resistance. Further, it has been found that the weight of the insulating curtain is largely unrelated to the heat insulating property, and the heat insulating curtain having a thickness containing a high internal air layer is excellent in the heat insulating property. In the future when manufacturing a heat insulating curtain, It is judged that it is desirable to manufacture a combination of heat insulating materials that contain a high internal air layer content and that can maintain the air layer even for long-term use while minimizing the volume.

To preserve the superior genetic resources and restore the endangered species, Somatic cell nuclear transfer (SCNT) has been used widely. In Korea, the research of dog cloning has made outstanding achievements including the production of the world`s first cloned dog. Sapsaree (Sapsalgae), the representative dog of Gyeongsan-si was designated as a Korea natural monument (No. 368). This male dog used in this study has azoospermia due to unknown cause. In this study, the aim was to confirm the cause of infertility in the cell donor dog and to evaluate the reproduction potential of dog cloning using infertile male dog by SCNT. First, to confirm the infertility of the cell donor dog, the reproductive history and the testis were evaluated. The breeding histology was not recorded in individual document. In histopathology, the Sertoli cell tumor was confirmed in biopsy of the cell donor dog after death. But, these tumors are predominantly in older dogs. Second, we produced the cloned dogs with the somatic cells of the infertile dog and the appearance was similar with the cell donor dog. Also, microsatellite analysis confirmed the genetic relationship between the cell donor and clone dogs. Third, the potential breeding capacity of the cloned dog was confirmed. In T4 assay, the normal dog (same age with cloned dogs), cell donor dog, and cloned dogs was investigated. The cell donor dog with azoospermia had very low T4 level, and cloned dogs showed higher level of T4 than normal dogs. In CASA, There was no significant difference in sperm motor ability between normal dogs and cloned dogs. As a result, cloned dogs produced by SCNT had no problem regarding the reproductive function of the testis. In AI experiment, the semen of clone dogs was used to fertilize a natural female bitch and was diagnosed pregnancy by ultrasonography. In total, 7 puppies were born by normal delivery (male: 3, female: 4). In conclusion, this study confirmed that the reproduction problem of non-genetic infertility can generate a normal descendant by SCNT. Also, the first successful research to restore infertile dogs was completed. Furthermore, SCNT would be useful for the restoration of endangered species and application of superior traits.