A reliable and selective liquid chromatography–ultraviolet detection method for determination of antiprotozoals (selamectin, doramectin and fenbendazol) has been described. HPLC separation of active constituents was achieved on various C18 columns using methanol, acetonitrile, 0.1% phosphoric acid, acetic acid and distilled water as mobile phase, with UV detection at 243, 245 and 224 nm. The analytical procedure has been successfully identified. The method was validated for specificity, linearity, accuracy, repeatability and intermediated precision. All calibration curves showed good linearity (R2 of 0.9999) within the concentrations ranges (0~200, 0~200 and 50~400 μg/mL). The accuracy and repeatability showed 99%, 100%, 100% and below 0.4%, 0.5%, 0.6%, respectively. The precision tests conducted for 3 days in three different concentrations with standard also revealed below 3.5%, 2.4% and 2.7%. The method has also been applied successfully to monitor post-market 5 veterinary products of which active ingredient are selamectin, doramectin and fenbendazol. There were no non-compliant products.

In general, the larvae of Protaetia brevitarsis is raised on fermented oakwood sawdust. Many attempts have been to changes components sawdust, such as using other tree instead of oakwood or adding another material. Like this some study exists, about 'component' of sawdust, however little is known of relation between larval growth and quality of sawdust. The phenomenon-big particle size sawdust flows up on excrement from the larvae-is occurring in used sawdust for raising the larvae. This study was conducted to observe the larvae and adult of Protaetia brevitarsis act according to each sawdust particle size. We conducted choice experiments with Protaetia brevitarsis, which were offered sawdust differing in particle size. Females preferred to lay their eggs on the fine particle sawdust. Also in the case of larvae, the more fine sawdust particle has the more high level of preference, and so is hatchability. Especially larvae tend to be slanted toward finer sawdust. Growth rate of the larvae was shown the highest level in the finest sawdust just as others do. These results show that fine sawdust particle size is advantage for larval development and oviposition.

In order to establish an indoor-rearing system for Copper butterflies; Large Copper butterfly, Lycaena dispar, the effect of temperature, photoperiod on larval development was investigated. As temperature increased, the developmental period was gradually reduced. The developmental periods of Large Copper larvae was 11.0 days and 28.5 days at 30℃ and 17.5℃, respectively. We investigated the sensitivity of stages to diapause induction. The experiment involved transfer of individuals from diapause averting (LD 16:8 h, 25℃) to diapause inducing condition (LD 8:16 h, 20℃) at various stages. Daipause was induced in 95.2% insect transferred at hatching larvae, in 15.6% of insects transferred at 2nd stadium molt, in 0% of insects transferred at after 3rd stadium molt. Percentage of diapause induction increased with the length of short days and low temperature. The main sensitive stage to photoperiodic and temperature induction of diapause determination was the early first larval instar. The diapause sensitivity began 14 days and ended 20 days after hatching larvae. The main stage sensitive to photoperiodic induction of diapause determination was the early first larval instar under low temperature and short day length. In relation to diapause termination, pupation was accelerated with cold treatment after diapausing 15 to 20 days and suitable chilling temperature was 8℃.