In 2010, a new variety for sawdust cultivation,was produced by monokaryotic-monokaryotic crossing between Sanjo 701ho and FMRI0995. The optimum temperature for Sanjo 708ho mycelial growth was 25oC; mycelial growth on potato dextrose agar was 51.6 mm over 7 days. The fruiting pattern was sporadic, and the optimal temperature range for fruiting was 5~20oC. Regarding fruiting body characteristics, the pileus was 65.8 mm in size, 16.1 mm in thickness, and hemispherical in shape. The stipe was 42.6 mm in and 19.6 mm in thickness. Mproductivity was good during thelow-temperature period from autumn to spring, with an average productivity of 310.7 g/bag.

Five empirical farmhouses were selected to reduce the high temperature damage in oak mushroom cultivation using bed-logs. The cultivation facilities were categorized as follows: those having two blackout curtains or one blackout curtain and outdoor oak mushroom cultivation. The inequality of the indoor condition, oak mushroom hyphae rampant ratio, and fruit body production in each test condition was evaluated. 3oC was lower in indoor temperature of cultivation facility having two blackout curtains than one blackout curtain. Specifically, the indoor air humidity average of cultivation facilities having one or two blackout curtains was 10% lower than that of outdoor oak mushroom cultivation. This condition is not ideal for oak mushroom cultivation as continuous indoor humidity control is essential for producing good fruit bodies. The Inoculated bed-log surface and oak mushroom hyphae rampant ratio of bed-logs cultivated with two blackout curtains was superior to other tested conditions. The mushroom production ratio observed in facilities with two blackout curtains was 117-204% higher than those cultivated in facilities with only one blackout curtain. Furthermore, the mushroom production ratio increased in based on these findings, we recommended five cultivation facility models to reduce high temperature damage in oak mushroom cultivation using bed-logs.

실내에서 육성된 117개 교배균주를 시험 재배하여 자실 체의 특성 및 생산성을 검정하고 결과가 우수한 07-55,07-66, 07-84, 07-93, 07-117균주를 선발하고 이들의 모균 주들과 확대 재배하여 모균주와의 생산성을 비교한 결과 각 균주의 생산성은 07-66균주가 36.1%의 회수율을 보여 가장 높게 나타났으며 07-117균주는 35.6%, 07-93균주는 27.1%, 08-84균주는 25.7%로 조사되었고 07-55균주는 가장 생산성이 낮은 6.5%로 조사되었다. 07-93 균주와 07-66균주는 생산성이 높으나 대가 긴 특성을 보였고 07- 117균주는 전체적인 자실체 품질이 다른 균주들보다 낮았 다. 또한 모균주와 교배균주간에 재배를 통한 온도형 분 석 결과 07-117균주는 고온형, 07-66 및 07-84균주는 중 온형, 07-55 및 07-93균주는 저온형으로 구분되었으며 07-93균주는 비교적 버섯발생온도범위가 넓은 가능한 균 주이나 품질이 저조하였다.

재배사 시설구조 조사결과 2~3중 구조로 재배하는 임가 가 96%로 외부의 환경변화에 대비한 안정적인 재배시설 구조를 갖추고 재배하는 것으로 조사되었으며, 여름철 재 배환경 변화를 분석하기 위하여 재배기간 온습도 변화를 조사한 결과 외부 백엽상의 온도는 20.2~29.9oC, 습도는 66.2~99.9%로 나타났다. 재배시설 내부의 고온 환경조절 결과 시험재배사 2에서 19.3~25.7oC, 습도변화 81.6~ 99.9%로 온도제어에 효과적이었다. 자실체 특성조사에서 시험재배사 2에서 갓직경이 66.2 mm, 대길이 54.1 mm로 품질이 우수하였으며, 배지당 수확량도 312 g/봉, 개체중 26.6 g으로 개선되었다. 냉방시설 사용에 따른 일간 전력 소비량은 56 kwh로 조사되었으며, 전력비용은 2,195원/일 로 산출되었다.

In order to develop of practical model of the sawdust cultivation of Lentinula edodes, we conducted experiments of the sawdust cultivation in different conditions of cultivation environment and shapes of sawdust medium. For different types of cultivation environments, we provided environmental control system facility, vinyl house and forest cultivation. We also chose the 2.6kg cylindrical type and the 1.5kg cylindrical type for shapes of medium. We decided cultivation on shelves for the 2.6kg cylindrical type sawdust medium and cultivation on the ground for the 1.5kg cylindrical type sawdust medium to cultivate the mushroom. The result showed that forest cultivation was the first place cultivated mushroom followed by environmental control system facility and cultivation vinyl house. Among 2 types of sawdust medium, the 1.5kg cylindrical type showed better quantity in terms of a fruit body of mushroom.

This study investigated physiological characteristics and genetic relationship of 30 strains of Lentinula edodes, collected from the Europe, Asia, North America and preserved in the Forest Mushroom Research Institute(FMRI). In physiological characteristics, Papua New Guinea strain was excellent mycelium growth in 25℃ for 7 days on PDA media. For all strains, the optimal temperature for mycelial growth, their tunicate and color of hypha were observed. It surveyed their mycelial growth on oak sawdust media in test tube and independence with inter-strains and cultivar developed in FMRI by strain's anastomosis culture. It was carried by RAPD using operon primers as molecular genetic methods, investigated genetic relationships among strains using UPGMA in NYSYSpc(2.1) according to the presence or absence of bands. <This research was supported by Technology Development Program for Agriculture and Forestry, Ministry for Food, Agriculture, Forestry and Fisheries>