Cucumber mosaic virus (CMV) poses a considerable threat to a diverse array of crops in global agriculture. CMV impacts commercially important cut lilies by diminishing both yield and flower quality. We used RNA sequencing (RNA-seq) to investigate the changes in gene expression in the leaves and bulbs of four distinct cultivars of cut lily, ‘Cancun,’ ‘Brunello,’ ‘Connecticut King,’ and ‘Casa Blanca’ following CMV infection. Notably, CMV affected photosynthetic processes by significantly downregulating genes associated with photosynthesis. In addition, CMV infection was detrimental to chloroplast function and energy production. We observed differential expression of genes associated with both dominant and recessive resistance pathways that are crucial for preventing virus entry, replication, and systemic spread within the plant. Based on functional annotation and differential gene expression analysis, we identified the regulatory genes involved in triggering immune responses, modulating signal transduction, and specific host factors during CMV infection. To validate the RNA-seq findings, we selected four genes involved in resistance, virus multiplication, and virus spread and analyzed them using real-time quantitative reverse transcription PCR (qRT-PCR) with specific primers. The qRT-PCR results aligned closely with those from RNA-seq, showing consistent fold-change responses for the genes that were differentially expressed, indicating that the RNA-seq results were reliable. These results deepen our understanding of the complex genetics behind plant-virus interactions while also providing information for breeding programs that aim to develop CMV-resistant lily cultivars.

As part of the 2024 research initiative, “Investigation and Discovery of Prokaryotes in Freshwater Systems,” samples were collected from diverse freshwater habitats, including both water and soil environments. Approximately 2,000 bacterial strains were isolated as single colonies and identified through 16S rRNA gene sequence analysis. Among these, 38 strains shared ≥98.7% 16S rRNA gene sequence similarities with those of known bacterial species not previously reported in Korea. These strains were thus categorized as newly recorded bacterial species in Korea. These 38 bacterial strains displayed significant phylogenetic diversities, spanning 2 phyla, 4 classes, 15 orders, 24 families, and 34 genera. These unrecorded species were classified into the following classes: Actinomycetia (with genera including Microcella, Conyzicola, Curtobacterium, Leucobacter, Microbacterium, Frigoribacterium, Lysinibacter, Streptomyces, Nonomuraea, Actinocorallia, Ruania, and Actinoplanes), Alphaproteobacteria (Paracoccus, Youngimonas, Loktanella, Corticibacterium, Neorhizobium, Onobrychidicola, Ferranicluibacter, Aureimonas, Asticcacaulis, and Novosphingobium), Betaproteobacteria (Rhodoferax, Rugamonas, and Cupriavidus), and Gammaproteobacteria (Rheinheimera, Shewanella, Kosakonia, Leclercia, Hafnia, Yersinia, Pseudomonas, Lysobacter, and Acinetobacter ). Further characterization included assessment of Gram reaction, colony and cell morphology, biochemical properties, and phylogenetic relationships. This report presents detailed phylogenetic and phenotypic characteristics of these bacterial species.

The recent release of contaminated water from the Fukushima Daiichi Nuclear Power Plant highlights the need for accurate tritium measurement, particularly near the minimum detectable activity (MDA) of 5 Bq·L−1 set by South Korea’s Nuclear Safety and Security Commission. This study aims to improve low-level tritium measurement accuracy by optimizing the region of interest (ROI) for quench curve determination. These adjustments are crucial for separating tritium signals from background noise. Quench standards were prepared and measured using a liquid scintillation counter (LSC). Three ROIs were analyzed to assess the impact of channel selection on measurement precision: A 20-148 channel range optimized via figure of merit (FOM) analysis, a 20-250 channel range covering tritium’s full beta spectrum, and a broad 1-1024 channel range. Quench curves were obtained by fitting the counting efficiency of each ROI to the quench standards. Tritium samples with six different activity levels were prepared, and their radioactivity was calculated using the quench curves. Selecting appropriate ROIs for quench curve determination is critical for measuring low-concentration tritium accurately. This approach reduces uncertainty and emphasizes reliable methods to improve the precision and consistency of tritium measurements.

The Yeongsan River is a prominent inland waterway, alongside the Han River, Nakdong River, and Geum River in South Korea. Numerous bacterial strains were isolated from the Yeongsan River basin for a comprehensive investigation into indigenous prokaryotic species conducted between 2020 and 2023. These bacterial strains were identified using 16S rRNA gene sequencing, wherein 45 bacterial strains shared >98.7% sequence similarities with bacterial species not recorded in Korea thus far. Therefore, this study aimed to catalogue aforementioned unrecorded species and characterize them contingent upon their Gram nature, colony and cell morphologies, biochemical properties, and phylogenetic positions. These bacterial species were determined to be phylogenetically diverse. They were categorized into nine classes, 18 orders, and 25 families. These previously unrecorded species were classified into the following genera and classes: Chitinophaga (class Chitinophagia); Flavobacterium (class Flavobacteriia); Rhodopseudomonas, Gemmobacter, Paracoccus, Azospirillum, Sphingomonas, Novosphingobium, Sphingorhabdus, and Erythrobacter (class Alphaproteobacteria); Bordetella, Pararobbsia, Polynucleobacter, Rhodoferax, Aquabacterium, Malikia, Comamonas, Ideonella, Paucibacter, Undibacterium, Cupriavidus, and Thauera (class Betaproteobacteria); Pectobacterium, Arenimonas, Lysobacter, and Luteimonas (class Gammaproteobacteria); Luteolibacter (class Verrucomicrobiia); Mycolicibacterium, Angustibacter, Ornithinibacter, Janibacter, Schumannella, Aurantimicrobium, Luedemannella, Nocardioides, and Propionicimonas (class Actinomycetes); Geothrix (class Holophagae); and Lactococcus (class Bacilli).

본 연구는 소래풀을 경관화훼로 이용하기 위해 온도조건에 따른 발아특성을 알아보고 회귀분석(bilinear, parabolic, beta distribution)모델을 통해 주요온도(최저, 최적 및 최고온 도)를 구명하고자 하였다. 소래풀 종자는 5, 10, 15, 20, 25, 30, 35℃ 항온 조건 중 25℃에서 약 6~7일만에 최종발아율이 100%에 도달하였으며, 발아세, 발아속도, 평균발아속도와 평균발아시간이 각각 100%, 21.37ea/day, 14.48, 4.39일 로 다른 처리보다 발아특성이 우수하였다. 이를 바탕으로 발아 속도(germination rate, GR)가 50%인 시점(GR50)을 역수로 (1/GR50)하여 주요온도를 분석한 결과, bilinear모델의 경우, 최저, 최적 및 최고온도는 4.8℃, 25.8℃, 35.6℃였으며 (R2=0.9566, p<0.001), parabolic모델은 최저온도 6.1℃, 최 적온도 21.6℃, 최고온도 36.7℃였다(R2=0.8818, p<0.001). 또한 beta distribution 모델의 주요온도는 최저온도 6.1℃, 최 적온도는 23.1℃, 최고온도 40.1℃였다(R2=0.9102, p<0.001). 본 연구에서 분석한 회귀모델 모두 0.1% 수준에서 통계적 유의 차가 인정된 것으로 보아 소래풀 종자의 발아 시 최저온도는 4.8~6.1℃, 최고온도는 35.6~40.1℃, 최적온도는 21.6~25.8℃ 이며, 50% 이상의 발아율을 기대하였을 때 온도의 범위는 20~25℃가 적합할 것으로 판단된다. 이와 같은 결과는 소래풀 을 이용하여 경관조성을 할 때 파종 및 발아시기를 예측할 수 있는 자료로 활용될 수 있을 것으로 판단된다. 그러나 경관조성 을 하는 현장에서 실질적인 도움을 제공할 수 있도록 발아의 주요온도 모델과 함께 식물의 생물계절 관점에서 추가적인 연구 가 필요할 것으로 판단된다.

장미 속은 오랜 시간에 걸쳐 잡종화, 배수화 및 육종 등을 통해 약 250개의 종과 30,000여 품종이 존재하는 진화 역사를 가지고 있으며, 다양한 분류체계로 구분되고 있다. 이에, 본 연구 는 장미 속 자원의 다양성을 평가하고 자생식물 해당화의 육종적 활용을 위해 수행되었다. 장미 속 자원의 형태학적 다양성을 확인하기 위해 장미 속 자원 303점에 대해 형태적 특성조사를 실시하고 해당화 및 장미 속 자원 29점을 선발하여 SSR 분석을 실시하였다. 추가적으로, 해당화의 육종적 활용을 위한 자원 6점 을 선발하고 화분 검정을 실시하였다. 본 연구의 결과, 장미 속 자원 303점의 다양성 평가 결과에서 유사한 형태적 특징을 지닌 자원끼리 7개 그룹으로 나뉘었다. 선발된 29개 자원의 형태학적 데이터와 분자학적 데이터를 이용한 군집 분석 결과, 데이터의 유사성을 보인 자원끼리 각각 5개, 4개 그룹으로 구분되었다. 또한, 혼합분석 시에는 3개 그룹으로 확인되었다. 분류 결과를 바탕으로 자생 식물을 이용한 정원장미 육종을 위해 각각 특성이 다른 해당화를 3점 선발하였고 장미 속 자원에 속하는 정원 장미 3점을 선발하여 총 6자원을 선발하였다. 선발된 자원의 화분 검정 결과, 종간 교잡체를 제외한 5가지 자원에서 90% 이상의 정상화분율을 확인하였다. 본 결과는 자생식물 해당화를 재평가 하고 정원 장미 육종 전략 수립에 도움이 될 것으로 기대된다.

본 연구는 디지털 영상 제작 프로세스에서 점점 중요한 역할을 하고 있는 생성형 AI의 발전과 그 영향을 탐구한다. GPT, GAN 및 기타 생성 알고리즘과 같은 모델의 개발에서 AI 기술의 급속한 발전으로 영상 제작 환경이 큰 변화를 겪고 있다. ChatGPT, Runway, DALL·E, MidJourney, SunoAI 등 생성형 AI 모델의 발전으로 영상 제작 단계에서 적용 가능성이 크게 확장되었다. 생성형 AI는 아이디어 기획에서부터 최종 편집 프로세스에 이르기까지 다양한 제 작 단계를 간소화할 수 있는 잠재력이 있다. 예를 들어, AI는 플롯 아이디어나 대화를 생성하 여 대본 작성을 지원할 수 있으며, 후반 작업에서는 시각 효과를 향상시키고, 사실적인 환경을 만들거나, 반복적인 편집 작업을 자동화할 수 있다. 또한, AI 기반의 사운드 디자인 도구는 영 상 분위기에 맞춘 음악과 사운드 효과를 자동으로 생성할 수 있다. 본 연구는 현재 사용되고 있는 생성형 AI 기술을 조사하고, 특히 런웨이 AI 영화제에서 소개된 사례들을 통해 그 장점 과 한계를 분석한다. 연구 결과, 생성형 AI는 영상 제작에 드는 시간과 비용을 획기적으로 줄 일 수 있는 잠재력을 지닌 반면, 저작권 문제와 딥페이크와 같은 기술적, 윤리적 문제는 신중 한 고려가 필요함을 제시한다. 향후 연구 과제는 AI와 인간의 예술적 창의성 간의 균형을 유 지하는 방법에 관한 것이다.

Geometric abstract art has been a persistent form since ancient times and is defined in modernity as a genre of abstract painting. According to previous studies, the geometric structure of traditional Korean costume possesses uniqueness based on Korean thought and philosophy. Therefore, the purpose of this study is to first derive the characteristics of Wilhelm Worringer’s theory, a theorist of geometric abstraction, and then compare the spiritual similarities between the traditional Korean costume and geometric abstract art, with the aim of examining the artistic competitiveness of Hanbok. Photographic materials from domestic and international museums, as well as books published by museums and government agencies, were analyzed. Further, the characteristics of Worringer's theory of the abstract impulse were derived. The results are as follows. First, the external factors are characterized by a single geometric shape on a flat surface with restrained spatial expression. The internal factors include anxiety, comfort from religion or ideology, and the need for a visual refuge for rest. Second, traditional Korean costume and geometric abstraction share common spiritual contexts of artistry, transcendence, and stability according to Worringer's theory. Third, the study of the spiritual similarities between the traditional Korean costume and geometric abstract art through Worringer’s theory reveals that the artistry of traditional Korean costume is expressed in universal forms, transcendence as intrinsic origins, and stability as a unique aesthetic consciousness. These findings provide a framework for reinterpreting the originality and universality of traditional Korean costume as Korean cultural content, infused with the characteristics of modern art.

We aimed to evaluate the effectiveness of ensemble optimal interpolation (EnOI) in improving the analysis of significant wave height (SWH) within wave models using satellite-derived SWH data. Satellite observations revealed higher SWH in mid-latitude regions (30o to 60o in both hemispheres) due to stronger winds, whereas equatorial and coastal areas exhibited lower wave heights, attributed to calmer winds and land interactions. Root mean square error (RMSE) analysis of the control experiment without data assimilation revealed significant discrepancies in high-latitude areas, underscoring the need for enhanced analysis techniques. Data assimilation experiments demonstrated substantial RMSE reductions, particularly in high-latitude regions, underscoring the effectiveness of the technique in enhancing the quality of analysis fields. Sensitivity experiments with varying ensemble sizes showed modest global improvements in analysis fields with larger ensembles. Sensitivity experiments based on different decorrelation length scales demonstrated significant RMSE improvements at larger scales, particularly in the Southern Ocean and Northwest Pacific. However, some areas exhibited slight RMSE increases, suggesting the need for region-specific tuning of assimilation parameters. Reducing the observation error covariance improved analysis quality in certain regions, including the equator, but generally degraded it in others. Rescaling background error covariance (BEC) resulted in overall improvements in analysis fields, though sensitivity to regional variability persisted. These findings underscore the importance of data assimilation, parameter tuning, and BEC rescaling in enhancing the quality and reliability of wave analysis fields, emphasizing the necessity of region-specific adjustments to optimize assimilation performance. These insights are valuable for understanding ocean dynamics, improving navigation, and supporting coastal management practices.

본 연구는 한국의 다양한 형태의 미디어 콘텐츠를 가리키는 용어인 K-콘텐츠 제작에 있어 생 성형 AI 활용에 관한 연구이며, 특히, 제작 전, 제작, 제작 후 등 영상 콘텐츠 제작의 모든 단 계에서 AI 기술의 혁신적인 영향을 강조한다. 대본 작성, 트렌드 분석, 캐스팅, 장면 편집, 시 각 효과 등의 작업에 AI 기반 도구를 활용함으로써 제작자는 창의성을 강화하고 효율성을 높 이며 제작 품질을 높일 수 있다. 주요 조사 결과에 따르면 생성형 AI는 반복 작업을 자동화하 고 참신하고 창의적인 아이디어를 생성하며 품질의 일관성을 보장함으로써 생산 프로세스를 크게 간소화할 수 있다. 예를 들어, AI 도구는 스크립트 작성 및 스토리보드 작성을 지원할 수 있으며, 얼굴 인식 알고리즘 및 지리공간 데이터 분석은 배우 캐스팅 및 촬영 장소를 추천하는 것이 가능하다. 포스트 프로덕션 과정에서는 AI 기반 비디오 편집 및 시각 효과 도구를 활용하 여 콘텐츠의 내러티브 응집력과 시각적 매력을 향상시키는 동시에 생성 오디오 도구는 맞춤형 음향 효과를 창조시킬 수 있다. 생성형 AI의 K-Contents 활용은 기획-제작-유통 등 전체 제작 과정에 도입이 예상되며 생산 비용 절감, 글로벌 시장 확대 등의 경제적인 측면에서도 상당한 영향을 미칠 수 있다. 본 논문은 K-콘텐츠 제작에서 생성적 AI의 잠재력을 모색하고 향후, K- 콘텐츠 산업이 글로벌 시장에서 더욱 성장하고 번영할 수 있는 시사점을 탐구한다는 점에서 의의를 찾을 수 있다.

Traditional medicine and herbal remedies are gaining popularity worldwide, comprising a significant portion of healthcare research, advancements, and market demand. Growing scientific evidence supports their substantial efficacy as pharmaceutical ingredients and dietary supplements in preventive healthcare. When developing pharmaceuticals, it is crucial to ensure that ingredients are free from side effects and toxicity in order to prioritize safety. Geckos, known as shou gong, are a diverse group of lizards that are widely utilized for treating various diseases in Korean Medicine. This study was conducted to assess the potential acute toxicity of a water extract Gekko gecko by a single oral dose in Sprague-Dawley rats. Twenty rats of each sex were randomly assigned to four groups (5 rats each). Test articles were administrated once by oral gavage to rats at dose levels of 0, 500, 1,000, or 2,000 mg/kg body weight. Mortality, changes of body weight, and clinical signs of gross observation were monitored for 14 days after dosing. At the end of a 14-day observation period, all animals were sacrificed and complete macroscopic and hematological examinations were performed. There was no dead animal or test article-related effect on clinical signs, body weight, or gross finding. Other specific changes were not found between control and treated groups in hematology. Results showed no adverse effect at a dose of 500, 1,000, or 2,000 mg/kg in rats. The minimal lethal dose was considered to be over 2,000 mg/kg body weight in rats.

This paper aims to investigate how and to what extent ‘critical’ global citizenship is reflected in middle school English textbooks in Korea. Framed within Freire’s concept of critical literacy, the study is concerned with analyzing the written texts in two English textbooks, with a focus on the issue of representations. Using critical content analysis, the research centers on unpacking how race, racism, or racialization, especially in the United States, is represented, and to what extent these representations may be associated with global citizenship education in English language learning. The major findings indicate a notable absence of sufficient sociohistorical and cultural contexts of race in the United States as presented in the concerned English textbooks. Based on the analysis, this paper calls for an expansion of the dimensions of critical global citizenship in English language learning settings, aiming to provide students with broader opportunities to question colonial discourse and challenge issues related to power and systemic oppression.