본 연구는 우리나라 Holstein의 번식형질에 대한 유전모수를 추정하기 위하여, 2009년부터 2013년까지 분만한 농협 젖소개량사업소의 검정자료 경산우 144,793두의 211,899개의 번식기록을 이용하였다. 7가지 형질의 유전 및 오차 (공)분산을 WOMBAT 패키지를 이용하여 분석하였다. CTFS, GL, NRR, NS, FSTC, DO 및 CI의 유전력은 각각 0.064, 0.060, 0.005, 0.014, 0.012, 0.021 및 0.021로 추정되었다. 형질들 간의 유전상관은 –0.910에서 0.995로 나타났다. NRR과 NS, NRR과 FSTC 간에는 부의 유전상관이 나타났으나, DC와 CI는 강한 양의 유전상관을 나타냈다. CTFS와 FSTC는 DO 및 CI와 유의하게 양의 유전상관관계를 나타냈다(0.479~0.862). CTFS와 FSTC 사이의 낮은 유전상관은 두 형질이 서로 다른 유전적 측면에 있다는 것을 의미한다. 본 연구는 번식능력 개량을 위한 기초 자료를 제공할 수 있을 것이라 판단한다.

목적 : 정상안에서 조도에 따른 눈의 굴절력과 동공 크기의 동적변화를 측정하고, 동공 중심점의 이동량과 방향을 알아보고자 하였다. 방법 : 2020년 7월부터 2020년 9월까지 경기도 지역 안경원에 원용 안경 처방을 위해 내원했던 안과적인 질환 및 각막 굴절 교정 수술 경험 없는 성인 29명(남 14명, 여 15명) 58안을 검사하였고, 평균 나이는 35.62±14.48세였다. 전자동 수차 분석기를 이용하여 조도에 따른 눈의 굴절력, 동공 중심점의 변화 값을 측정하 였고, 각각 3회씩 측정하여 평균값을 사용하였다. 통계 프로그램 SPSS를 이용하여 조도 변화에 따라 명소시와 암소시에서의 굴절력, 그리고 동공 중심의 이동량을 비교 분석하였다. 결과 : 조도에 따라 구면 굴절력은 –2.80 D에서 -2.94 D로 평균 –0.14 D 만큼 변화하였다(p<0.001). 원주 굴 절력은 –0.93 D에서 -0.98 D로 평균 –0.05 D가 증가하였다(p<0.001). 동공 중심점의 평균 이동량은 수평 방향으로 0.14 mm, 수직 방향 0.13 mm만큼 이동하였다. 방향 별 동공 중심점 이동은 양안에서 상측 35안(60.3%), 하측 20안(34.5%), 코측 25안(43.1%), 귀측 33안(56.9%) 이동하였다. 양안에서 동공 중심점 이동은 수평방향으 로 높은 상관관계가 있었고(r=0.700), 수직방향으로는 낮은 상관관계가 있었다(r=0.244). 결론 : 조도가 어두워질수록 구면굴절력은 (-)방향으로 커지고, 동공 크기가 증가하며, 동공 중심은 수평과 수직 방향으로 모두 이동하였다. 따라서 야간 활동 안경렌즈를 조제할 때는 굴절력 변화와 동공 중심 이동에 따른 시력의 질적 저하를 고려해야 할 것으로 생각된다.

An experiment was carried out to assess the effect of feed selenium-lysine (Se-Lys) supplementation on milk compositions and serum biochemical indices in Saanen dairy goats in Korea. A total of twelve 36 months old Saanen lactating dairy goats (47±6.21 kg) fed the similar dry matter intake twice a day at 2% of BW (DMI) (10.9% moisture of concentrate and 19% moisture of roughage), milk yield (2.5 kg/d) and parity (2) were randomly selected and subjected for the present study, divided into two groups with six goats in each group. The goats in the control group received rice hulls (10 g/ day) only, and did not receive Se-Lys; goats in the treatment group were fed 0.06 g of Se-Lys with 10 g of rice hulls every day before feeding roughage for six weeks. The milk sample was collected every week, and its compositions were analyzed. The results of the present study showed that there is no significantly increased milk production in Se-Lys treated group goats when compared with control group goats. But, Se-Lys treatment significantly increased the milk protein content (3.98±0.16%), fat (3.72±0.27%), lactose (4.07±0.14%), total solids (12.51±0.28%) and urea (14.42±1.45 mg/dl) content as compared to the control group goats (p<0.05). The somatic cell counts (207,740±28.81 cells/ml) were significantly lower in the Se-Lys treated group than in the control group (p<0.05). Also, the results of the current study showed that supplementation of Se-Lys were significantly decreased the blood biochemical indices of IL-6 (34.34±6.04 pg/ml), TNT-α (0.56±0.22 ng/ml), MDA (5.07±1.03 ng/ml), GPx-1 (9.07±5.17 ng/ml), sCD4 (2.64±1.02 ng/ml) and sCD8 (5.08±2.08 ng/ml) level when compared with without addition of Se-Lys group dairy goats (p<0.05). On the other hand, the selenoprotein P (1,580.18±127.62 ng/ml) level was significantly higher in Se-Lys supplemented group than in the control group (p<0.05). Based on the study results, it was concluded that feed Se-Lys supplementation may improve milk yield with positively improved protein, fat, lactose, total solids, urea content, and biochemical indices without negative effects on milk production traits.

Although some factors, including season, age, type of estrus (natural estrus vs. induced estrus) and semen type (conventional vs. sexed), affect the conception rate following artificial insemination (AI) in dairy cattle, there is little information about the influence of ovarian characteristics, such as preovulatory follicle (PF) location at estrus, on fertility in dairy cattle. In most breeds of cattle the right ovary appears to function more actively than the left and about sixty percent of pregnancies in dairy cattle occur in the right horn of uterus (Reece and Tuner, 1938). Our study aimed to compare conception rates in dairy cattle between PFs that developed in the left ovary and those that developed in the right ovary at estrus. In this study, we examined the locational effect (left or right ovary) of the preovulatory follicle (PF) on fertility in dairy cattle. In total, 955 artificial inseminations (AI) were analyzed. At AI, PF locations were examined using rectal palpation, and dairy cattle were divided into two groups on their PF locations: (i) the PF located in the left ovary (L-PF); and (ii) the PF located in the right ovary (R-PF). Pregnancy was diagnosed by rectal palpation or ultrasonographic examination 60 days after AI. The conception rate was 38.1% in all dairy cattle. Conception rate was higher in the R-PF (40.8%) than in the L-PF (33.2%). In summary, PF development in the right ovary was associated with increased conception rates in dairy cattle.

The boar sperm has more lipid droplets and specialty of seminal plasma compared with other species, causing difficulties of freezing sperm and decreases for the utilization of frozen semen into the artificial insemination. However, several studies reported significant results for the recovery of sperm motility and reproductive by addition of cryoprotectants and seminal plasma after thawing. This study was designed to investigate the effects of supplementation of trehalose or glycerol in the LEY (lactose and egg yolk in BTS) solution for the conventional freezing and vitrification process. Two boars aged 16 months were used to collect semen for 2 times in a week. The samples were allotted to 3 freezing solutions (LEY + glycerol 10.5% + OEP 1.5%, LEY + trehalose 1M + OEP 1.5%, and sucrose 1.5M + trehalose 1 M + OEP 1.5%) after centrifugation at 800 g for 10 minutes. Semen was equilibrated in freezing solutions for 10 minutes and injected into plastic straws with 2∼3 air bubbles to minimize freezing damages. Vitrification was performed to locate sperm in 5 cm above LN2 for 5 minutes, and the conventional freezing was conducted with an automatic freezer. Motility and survival rates were measured by CASA (Computer assisted sperm an alyzing system) and FITC (Fluorescein isothiocyanate), respectively after thawing semen at 50℃ for 12 seconds. The results were analyzed by ANOVA with STATVIEW statistical program. The vitrificatioin solution (LEY + 10.5% glycerol + 1.5% OEP) presented higher motility (20.9%) than other solutions while the solution (LEY + 1M trehalose + 1.5% OEP) showed the lowest (motility : 5.2%). However, survival rates of vitrified sperms detected by FITC showed 1~4% live sperms in almost of dead sperms at all vitrification solutions’ groups, but survival rate of freezing solution of LEY + 1M trehalose + 1.5% OEP LEY and LEY + 10.5% glycerol + 1.5% OEP were showed 49%, and 79%, respectively. There were differences (P<0.05) survival rate of conventional freezing in LEY + 10.5% glycerol + 1.5% OEP and LEY + 1M trehalose + 1.5% OEP and the remaining showed no differences. The results suggested that vitrified boar semen was not enough to be utilized for the artificial insemination, but it showed possibility to utilize for ICSI and conventional freezing with glycerol would be useful method for artificial insemination in pig while we choose the outstanding semen against tolerance to freezing damages.

Bioenergy is classified to one of the renewable energy resources such as solar, wind, hydro and tidal energies. It should be noted that all the renewable energies contribute to the reduction of greenhouse gases emission. In some cases, energy from wastes was also categorized as a renewable energy in our country even though it has only negligible effect on the emission reduction. In this paper, we tried to identify the bioenergy in order to follow the global indices of the renewable energy. The indices evaluated here were whether a resource is renewable, iogenic, biodegradable, combustible and organic. Biogenic and combustible were selected as the indices to identify the bioenergy. It was also suggested that combustible as an index can be exchangeable to organic.

This study was carried out to investigate synthetic extender for semen cryopreservation of Jeju Native Black Bull. The semen was collected using an artificial vagina and transported to the laboratory. The semen was diluted 1:1 by Tris-Egg yolk extender and contrifuged in 1,500 rpm for 15 minutes. The supernatant was removed. The pellect was diluted to final sperm concentration of 2×108/ml by doubling in every 30 minutes at 4℃ cold chamber. The semen was equilibrated for 4 hours at cold chamber and packed to 0.5 ml straw. The semen straws were located above 5 cm for 10 minutes. The height and duration affect the freezing speed by temperature. The frozen straw was plunged to LN2. The presented straws were examined the viability and motility after thawed at 37℃ water bath. Frozen-thawed sperm were evaluated sperm viability, membrane integrity and acrosome integrity. Post-thawed sperm viability has been significantly higher (p<0.05) in fresh sperm (93.27±1.62%) than frozen-thawed sperm (73.34±3.27%). However, there were no significant differences between fresh and frozen-thawed dead cell rate (7.35±2.63 vs, 13.71±2.85). In sperm motility, between Triladyl and AndroMed Extender, there was no significant different (72.86±2.83 vs, 81.47±2.48), similarly, the dead cell rates was similar (18.41±3.42% and 17.26±4.25). The results of our study suggest that AndroMed to the freezing extender showed more positive effect on the frozen-thawed spermatozoa in Jeju Native Black bull semen.

해양경찰 경비함정에서 근무하는 경찰관, 전경 등 승조원이 바다로 추락하는 사고가 발생할 경우 CCTV 영상을 실시간으로 분석하여 경보를 발령하고 즉시 구조할 수 있는「함정 승조원 추락 경보 시스템」을 개발하였다. 길게는 7박 8일의 출동 임무를 수행하는 함정요원들은 수시로 급변하는 해상기상의 영향을 많이 받는 함상생활 중 불의의 추락사고를 당할 위험에 노출 돼 있는 것이 사실이다.

The aim of this study was to evaluate the effect of α-tocopherol on blastocysts development and subsequent cryosurvival of the vitrification. The α-tocopherol(0, 100, 200, 400 μM) was added in to culture medium for the bovine embryos. The blasocysts from the α-tocopherol and untreated control groups were then frozen-thawed, and their cryosurvival was assessed by in vitro culture for 48 h. There were no differences in the overall cleavage rate(56.14±4.66, 58.18±4.70, 62.97±6.86 and 51.17±7.28) among four treatment groups. However, in blastocyst development and total cell number were significantly higher in α-tocopherol 200 μM(38.60±7.12; 106.33±3.50) to culture medium than other treatment groups(29.30±5.24, 31.60±7.12 and 26.37±4.18; 101.36±5.12, 97.27±2.87, and 91.23±7.52 respectively). Before and after vitrification, the total cell number and blastocyst development of embryo were significantly higher in July to August than September to October. In conclusion, addition of α-tocopherol 200 μM to in vitro bovine embryo culture medium was beneficial for improving embryo quality by decreasing the embryo damage blsstocysts cell number and improving the tolerance of the embryos to cryopreservation.

명량대첩(鳴梁大捷)은 1597년 음력 9월 16일(양력 10월 25일) 정유재란 당시 충무공 이순신의 지휘를 받는 조선 수군 전함 13척이 일본 수군 전함 133척을 물리친 세계 해전사에 빛나는 전투이며, 정유재란의 판도를 바꾼 대첩이다. 이충무공은 13척의 조선 수군 전선으로 수백 척의 적선을 대적하는 데에 있어서, 명량수도의 좁은 지형과 독특한 조류를 이용하고자 하여 9월 15일에 해남 우수영으로 진영을 옮긴 다 음 날에 명량해협에서 적선을 맞이하였다. 본 연구에서는 명량대첩 당시에 조ㆍ일 양국 수군의 전함 기동에 큰 영향을 준 명량해협의 조류 상 태를 추산하기 위한 분석을 수행하였다. 연구에 있어서는 평형조석론(equilibrium theory of tide)과 동력학적 조석론(dynamical theory of tide) 을 기반으로 하여, 지구에 대한 천체(달, 태양)의 위상이 명량대첩 당시에 있어서의 위상과 동일하게 되는 회귀시점을 천체 운동의 주기를 분석 함으로써 계산하였다. 그리고 그 회귀시점에 대하여 조화분석법으로 계산한 조석 자료를 적용하는 방법으로 명량대첩 당시의 조류 상태를 추산 하였다. 그 결과, 명량수도의 조류는 새벽 06시 36분에 전류해서 북서쪽으로 흐르기 시작하여 09시30분에 최대 유속인 8.3노트에 이르렀고, 12 시 48분에 다시 남동쪽으로 전류하여 16시 12분에 최대 유속인 9.9노트에 도달한 다음, 19시 06분에 북서쪽으로 다시 전류하였다고 판단할 수 가 있었다.