We investigated 18 amino acid contents in the fruiting bodies of Agaricus strains to understand their variation in different growth temperature. Although total content of amino acid was more or less different depending on the isolates, it increased until the temperature was 22℃ and decreased above 22℃. Commercial strains of A. bisporus, such as Yangsongi-505, Yangsongi-705 and commercial strain A collected in the market exhibited these general trend in total amino acid content. However, another commercial strain B did not follow these trend. A strain of A. bitorquis did not produce mushrooms at 10 and 13℃, but mushrooms harvested in the other treated temperatures exhibited with the similar patten. Generally, the most amino acid contained in Agaricus spp. was cysteine and followed by phenylalanine, glutamic acid, lysine, proline and histidine. We detected two trends for the contents of each amino acid in the fruiting bodies of Agaricus spp. grown at the different growing temperature. Some amino acid contents were largely correlated with the growth temperature, but the others showed the trend of increase until 22℃ and decrease above 22℃.

To understand the saccharide contents and quantity of winter mushroom (Flammulina velutipes) according to its growth temperature, we measured the saccharide contents at different growth temperature. In our results, the saccharide of its fruiting body turned out to be mainly composed of xylose, trehalose and mannitol in all treatments. In the other hand, Ribose, myo-inositol and sucrose were detected in some treatments. The quantity of trehalose decreased as the growth temperature increased with a variation of its quantity depending on the isolates used in the experiments. In the case of xylose and mannitol, detected in all treatments, the pattern of their quantities was not possible to be profiled and the pattern might be largely depending on the isolates. However, the quantities of xylose and mannitol were largely in a direct proportion and the fluctuation of their quantities was congruent with the exception of ASI 4103, ASI 4166 and ASI 4065. The xylose quantity of ASI 4103 and ASI 4166 increased until the temperature was up to 10℃ and decreased when the temperature was above 10℃. That of ASI 4065 decreased as the temperature rose and increased when above 13℃. The mannitol quantity of ASI　4065 and ASI 4166 decreased until the temperature was up to 10℃ and increased when the temperature was above 10℃. That of ASI 4103 increased as the temperature rose and decreased when above 13℃.

We investigated 18 amino acid contents in the fruiting bodies of Agaricus strains to understand their variation in the harvesting periods. Total content of amino acid was different depending on the isolates. In the case of A. bitorquis and A. brunnescens, the total amino acid content increased and correlated with the harvesting periods. For commercial strains of A. bisporus, such as Yangsongi-505, Yangsongi-705 and commercial strain A collected in the market, it increased until the second harvesting period, but decreased in the third harvesting period indicating that these three isolates exhibit the exceptions for the general trend. Among isolates we used in our experiments, the isolate of A. brunnescens contained the highest and two isolates of A. bisporus were the lowest in total amino acids. We found two trends for the contents of each amino acid in the fruiting bodies of Agaricus spp.. One is the same with the trend of the total amino acid content and the amino acid content were largely correlated with the harvesting period. The other is the trend of increase of amino acid until the second harvesting period and decrease of amino acid in the third harvesting period, which was also shown in the total amino acid content of A. bisporus. Generally, the most amino acid contained in Agaricus was cysteine and followed by phenylalanine, glutamic acid, lysine, proline and histidine.

팽이버섯 병재배에서 재배사 내의 탄산가스 농도를 조절하여 소비자 요구에 맞는 버섯을 안정적으로 생산하고, 생육과 수확에 관련하여 적정기준 설정을 위해 실험을 수행한 결과이다. 공시균주 특성 값을 종합해보면 탄산가스 농도가 높아지면 초발이소요일수, 생육일수, 수확일수는 증가하는 경향이고, 백색계열보다 갈색계열이 탄산가스 농도에 상관없이 초발이소요일수, 생육일수, 수확일수가 전체적으로 짧으며, ASI 4103 균주와 같이 균주의 유전적 형질이 재배적 특성에 많은 영향을 주는 것으로 판단된다. 병당 수량성은 균주에 따라 처리농도에 따른 증감의 차이는 있으나, 전반적으로 탄산가스 농도가 높아지면 수확량은 감소하였다. 개체중은 균주에 따라 약간씩 차이는 있으나 대체적으로 탄산가스 농도가 증가하면서 약간씩 감소하는 경향이다. 병당 개체수에서는 균주간의 차이가 커서 탄산가스 농도 증가에 따른 일정한 경향을 확인 할 수 없었다. 자실체의 수분함량은 균주에 따라 약간의 차이는 있으나 탄산가스 농도의 증가에 따라 수분함량이 감소되었다.

Mushroom is cultivated in many areas of the Korea as one of the major economical crops. The production areas have steadily increased approximately 3,674 ha in 2002 to 4,118 ha in 2005. Several bacteria have been known as the causal agents of certain diseases of cultivated button mushroom, Agaricus bisporus, and oyster mushroom, Pleurotus ostreatus. It is well known as bacterial diseases of the cultivated mushroom such asbrown blotch, mummy disease, bacterial pit, bacterial rot and weeping disease, ginger blotch, and drippy gill. Unknown soft rot bacterium was isolated from sunken browning symptom of cultivated oyster mushrooms grown in Korea. The symptoms are appear as a sunken browning lesions on the caps of affected mushrooms. The bacterium causes a rapid soft rot of cultivated mushrooms in comparison with brown blotch bacteria at temperatures above 25℃. From these lesions we isolated one bacterial strain (designated OM1). Inoculation of bacterial isolates into mushroom caps yielded characteristic sunken brown, watersoaked and severe soft rot symptoms, but which were indistinguishable in early stage from those of the bacterial brown blotch well known to mushroom growers. Results of Gram stain and biochemical tests identified this isolate as Burkholderia gladioli pv. agaricicola. This was confirmed by pathogenicity, physiological and biochemical characteristics, and results of an analysis of the 16S rRNA gene sequences and the fatty acids profile. This is the first report of the isolation of B. gladioli pv. agaricicola from cultivated oyster mushroom in Korea.