In this study, we used dried ginger ethanol extracts which had been used for alleviation of pain and antipyretics for a long time, and confirmed anti-inflammatory and anti-oxidative activities among various pharmacological actions of dried ginger. The result of antioxidant activity using DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical scavenging activity, dried ginger shows concentration-dependent radical scavenging activity and more than 80% radical scavenging activity at concentration of dried ginger above 1 mg/ml. Also, in the mouse experiment in which gluten intolerance was induced by providing gluten meal, the effect of inhibiting weight loss was confirmed comparing administrated gluten group and dried ginger administration group. In addition, IL-4, an allergic cytokine, was measured in the small intestine and serum using ELISA (enzyme-linked immunosorbent assay), the results showed that IL-4 production in the dried ginger administration group was significantly reduced compared with the administered gluten 200 mg/ml group. In case of IL-6 and TNF-α production, which are inflammatory cytokines, decreased in the primary culture cell treated with lipopolysaccharide (LPS) for induction of inflammation after treatment with dried ginger in a dose-dependent manner. As a result, it was confirmed that the dried ginger extract has a high antioxidant activity and has a mitigating effect on the gluten intolerance which is an inflammatory allergic reaction.

본 연구에서는 참마와 명아주의 항산화 및 항염증 효능을 평가하기 위해 참마와 명아주 에탄올 추출물을 이용하여 free radical 소거활성, enzyme-linked immunosorbent assay (ELISA) 실험을 수행하였다. 참마와 명아주 추출물의 free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) 소거활성(EC50)에서 각각 2.386, 0.524 mg/mL로 측정되었다. 또한 참마와 명아주 추출물의 혼합 시료의 free radical 소거활성은 참마 추출물 : 명아주 추출물 비율이 2 : 1일 때 가장 뛰어난 것으로 나타났다. IL-6와 TNF-α의 ELISA 실험을 통해 항염증 효능을 평가한 결과, 마우스 비장세포에서 IL-6의 경우 1 mg/mL 농도에서 참마 추출물은 대조군과 대비하여 27.17% IL-6 생성을 감소시켰으며, 명아주 추출물은 72.30%의 감소를 나타내었다. TNF-α의 경우 참마 추 출물은 61.97%, 명아주 추출물은 77.85%로 유의성 있는 TNF-α생성 감소 효능을 나타내었다. 이 결과들을 통하여 참마와 명아주 추출물은 항산화, 항염증 효능을 가지고 있으며, 이를 활용하여 항염증에 효과가 있는 천 연물 제제에 응용 가능성이 있음을 확인하였다.