Italian ryegrass (Lolium multiflorum Lam., IRG) is a widely cultivated winter forage crop known for its high yield and nutritional value. This study evaluated the processing characteristics and feeding performance of IRG-based pellets in Hanwoo cattle (Bos taurus coreanae) and Korean native black goats (Capra hircus). IRG was harvested at the optimal growth stage and processed into two pellet formulations: IRG ≥80% (with up to 20% soybean meal) and 100% IRG. Feeding trials were conducted under ad libitum feeding conditions. Hanwoo cattle showed higher intake of 100% IRG pellets (7.9 kg/day/head) than IRG ≥80% pellets (7.5 kg/day/head, p<0.05), with similar average daily gain (0.9 ± 0.4 kg/day/head). Conversely, black goats exhibited significantly lower intake of IRG ≥80% pellets (54.6 g/day/head) compared to 100% IRG pellets (266 g/day/head), likely due to reduced palatability associated with soybean meal inclusion. These findings suggest that IRG pellets are suitable for Hanwoo cattle, while further optimization of pellet size and formulation is required to improve acceptance in goats. Future studies should assess long-term impacts on digestion, rumen fermentation, and metabolic responses.

온실 내부 환경은 지역에 따라 외부 환경의 영향을 지속적으로 받는다. 본 연구는 몽골, UAE(아부다비), 호 주(퀸슬란드) 등 지역별로 구축된 한국형 스마트 온실의 환경 특성을 비교하고자 수행하였다. 몽골과 아부다비의 온실 모두 내외부 엔탈피 차이가 감소함에 따라 환기율이 증가하였다. 아부다비의 반밀폐형 온실에서는 10시부터 14시까지 평균 내부 기온이 외부 기온보다 약 7－10°C 낮았고 내부 VPD(12mbar)는 외부 VPD(56mbar)보다 4.6 배 낮았는데 이 결과는 포그 시스템 운영과 관련이 있는 것으로 보인다. 퀸즐랜드 온실의 경우, 내부 온도가 외부 온 도보다 11시 기준 약 3.81°C 높았고, 내부 엔탈피와 VPD가 외부 온도보다 높았으며, 내부와 외부의 엔탈피 차이가 증가함에 따라 환기율이 증가하였다. 이 결과로 엔탈피를 낮추는 것은 환기와, VPD를 낮추는 것은 포그 시스템 작 동과 관련이 있는 것을 알 수 있다. 또한, 작물 생육에 적합한 환경 조건을 효과적으로 관리하기 위해 엔탈피와 VPD 기반의 포그, 환기 또는 난방 시스템이 필요하다는 것을 알 수 있다.

본 연구는 아메리카동애등에(Hermetia illucens)의 대량 사육을 위하여 남은음식물 사료의 최적 수분 함량과 적정 사육 밀도를 조사하였다. 실 험에서는 500 g의 습식 사료의 수분 함량을 각각 30%, 50%, 70%, 90%로 조정한 후, 3,500마리의 3령 유충을 7일 동안 사육하였다. 그 결과, 90% 수분 함량에서 평균 유충 무게는 7.19 g으로 측정되었고, 70% 수분 함량에서 가장 높은 무게인 10.54 g이 나타났다. 반면, 50%와 30% 수분 함량에 서는 유충 생육이 저조한 결과가 나타났다. 적절 사육 밀도를 조사를 위해 100,000, 150,000, 200,000, 250,000 마리의 3령 유충을 70% 수분함량 의 100 kg 습식 사료에 7일 동안 처리하였다. 그 결과, 250,000 마리에서 총 유충 생산량이 22.79 kg으로 가장 높게 나타났으나, 150,000, 200,000, 250,000 마리 밀도 간에는 유의미한 차이가 없었다. 100마리당 평균 무게는 100,000 마리에서 16.90 g, 150,000 마리에서 15.20 g, 200,000 마리 에서 11.30 g, 250,000 마리에서 9.50 g으로 확인되었으며, 100,000 마리와 150,000 마리 밀도 간에는 유의미한 차이가 없었다. 결론적으로, 남은 음식물 사료인 습식사료 100 kg를 급여할 경우 수분 함량을 70%로 유지하고 사육 밀도를 15만 마리로 설정하는 것이 가장 효과적임을 확인하였다.

Pluripotent stem cells (PSCs) are undifferentiated cells with the potential to develop into all cell types in the body. They have the potential to replenish cells in tissues and organs, and have unique properties that make them a powerful tool for regenerative therapy. Embryonic stem cells (ESCs) derived from the inner cell mass of the blastocyst of pre-implantation embryo and epiblast stem cells (EpiSCs) derived from the epiblast layer of post-implantation embryo are the well-known PSCs. These stem cells can differentiate into any of three germ layers of germ cells (endoderm, mesoderm and ectoderm). Additionally, induced pluripotent stem cells (iPSCs) refer to adult somatic cells reprogrammed to return to the pluripotent state by introducing specific factors. This is a breakthrough in stem cell research because ethical concerns such as fertilized embryo destruction can be avoided. PSCs have tremendous potential in treating degenerative cells by generating the cells needed to replace damaged cells, which can also allow to generate specific cell types to study the mechanisms of the disease and create disease models that screen for potential drugs. However, if the proliferative capacity of PSCs is not controlled, there is a risk that tumors will form, as this can lead to uncontrolled growth in their proliferative capacity. In addition, when PSCs are used for therapeutic purposes, there is a risk that the body’s immune system rejects the transplanted cells when the transplanted cells do not originate from the patient’s own tissue. Taken together, PSC is the foundation of stem cell research and regenerative medicine, providing disease treatment and animal development understanding. We would like to explain the classification of PSCs based on their developmental potential, the types of PSCs (ESCs, EpiSCs and iPSCs), their pluripotent status (naïve vs. primed) and alkaline phosphatase (AP) in PSCs and PSCs in domestic animals.

Ectopic ureter refers to a congenital anomaly in which one or both ureters do not connect to the urinary bladder at the correct anatomical site. This case report discusses the case of a 6-year-old female mixed-breed dog diagnosed with chronic urinary incontinence, systemic hypertension, pancreatitis, and sepsis resulting from an ectopic ureter. Treatment involved an initial nephro-ureterectomy to address severe pyonephrosis, followed by ureteroneocystostomy for the remaining functional kidney. Post-surgical outcomes showed notable improvements in clinical symptoms, laboratory findings, and blood pressure. This report emphasizes the need for early diagnosis and appropriate surgical treatment in cases of ectopic ureter. Additionally, it aims to present the clinical symptoms and conditions resulting from prolonged disease progression, as well as the corresponding treatment methods and prognosis.

Background: Pluripotent stem cells (PSCs) are capable of differencing into various cell types in the body, providing them valuable for therapy of degenerative diseases. Patientspecific treatments using PSCs, such as mesenchymal stem cells in patient’s own body, may reduce the risk of immune rejection. Inducing the differentiation of PSCs into vascular endothelial cells (ECs) altering culture conditions or using specific growth factors is able to applied to the treatment of vascular diseases. The purpose of this study was to induce the differentiation of porcine epiblast stem cells (pEpiSCs), bone marrow-derived mesenchymal stem cells (pBM-MSCs) and adipose-derived mesenchymal stem cells (pAMSCs) into ECs and then examine the functionality of vascular ECs. Methods: Porcine pEpiSCs, pBM-MSCs and pA-MSCs were induced to differentiate into ECs on matrigel-coated plates in differentiation medium (EBM-2 + 50 ng/mL of VEGF) for 8 days. Cells differentiated from these stem cells were isolated using CD-31 positive (+) magnetic-activated cell sorting (MACS) and then proliferated in M199 medium. Evaluation of ECs differentiated from these stem cells was treated with capillary-like structure formation and three-dimensional spheroid sprouting assay. Results: Porcine pEpiSCs, pBM-MSCs and pA-MSCs showed similar expression of pluripotency-related genes (OCT-3/4. NANOG, SOX2). These stem cells were differentiated into vascular ECs, but showed different morphologies after the differentiation. Cells differentiated from pEpiSCs showed an elongated spindle-like morphology, whereas cells differentiated from pBM-MSCs showed a round pebble-like morphology. In the case of pA-MSCs, these two morphologies were mixed with each other. Additionally, vascular ECs differentiated from these stem cells showed different formation of capillary-like structure formation and three-dimensional spheroid sprouting assay. Conclusions: Cells differentiated from pEpiSCs, pBM-MSCs and pA-MSCs presented the functionality of different vascular ECs, demonstrating the potential of the excellent ECs differentiated from pEpiSCs.

Background: This study explores the potential of discarded male layer embryos as a sustainable and non-GMO cell source for cultivated chicken meat production. The research aims to identify efficient methods for isolating muscle progenitor cells (MPCs) with high proliferative potential by conducting transcriptome analysis on thigh muscle tissues from both male and female chick embryos. Methods: Transcriptome analysis was performed on the thigh muscle tissues of male and female chick embryos, aged 12-13 days, (n = 4 each), to investigate the gene expression profiles and identify strategies for efficiently isolating MPCs. This approach aims to pinpoint techniques that would allow for the selection of MPCs with optimal growth and proliferation capabilities. Results: Using heatmap, hierarchical clustering, and multidimensional scaling (MDS), we found no significant sex-based differences in gene expression, except for the overexpression of the female-specific gene LIPBLL. The expression of muscle stem cell factors, including PAX3, PAX7, and other myogenic regulatory genes, showed no significant variation. However, to recover MPC-rich cells isolated from male thigh muscle, we found that by the pre-plating 7 stage, myogenesis-related genes, MYHs and MUSTN1 were minimally expressed, while the cell cycle arrest gene CDKN1A sharply increased. Conclusions: Our findings suggest that simple cell isolation directly from tissue is a more scalable and efficient approach for cultivated meat production, compared to labor-intensive pre-plating methods, making it a viable solution for sustainable research and resource recycling.

This study evaluated the efficacy of a wild boar repellent (Repellent A) consisting of tannins and plant oils (castor oil, garlic oil, and cinnamon oil). Sixty farmed wild boars (4-8 months old) were divided into three groups: the normal control group (NC, n=20), the experimental group (EP, n=20), and the comparative experimental group (C-EP, n=20), which used Repellent B consisting of guaiacol, eugenol, menthol, thymol, and indole. EP and C-EP were equipped with four repellents per feeder, while no repellents were installed in the NC feeder. The feed intake and the number of feeding approaches were measured for one week in all groups. The number of approach of wild boars in feeders was monitored daily using a CCTV camera. The daily feed intake per farmed wild boar in EP and C-EP was significantly decreased compared to NC (p<0.05), and EP was significantly decreased compared to C-EP (p<0.05). In the average number of daily approaches, EP and C-EP were significantly decreased compared to NC (p<0.05), and EP was significantly decreased compared to C-EP (p<0.05). In conclusion, Product A has been confirmed to have excellent repellent effects on wild boars, and it could be used to prevent wild boars from approaching pig farms.

Recently, ESG(Environmental, Social, Governance) has been recognized as an important factor for the sustainable growth of companies. However, only 14.5% of food manufacturing companies have adopted ESG management. In particular, small and medium-sized enterprises(SMEs) face difficulties in implementing ESG management due to a lack of specialized personnel and resource constraints. The purpose of this study is to analyze the impact of ESG ratings on the management performance of 40 food manufacturing companies listed on the Korea Exchange(KRX) that have been evaluated for ESG. The one-way ANOVA was used and performed on data for 40 food manufacturing companies published by the Korea Institute of Corporate Governance and Sustainability(KCGS) in 2023. The results of the analysis showed statistically significant differences in sales (F=12.936, p<0.001) and foreign ownership (F=7.74, p<0.01) based on ESG ratings. Furthermore, Scheffe's post-hoc analysis indicated that the higher the ESG rating and individual scores, the better the overall management performance. Therefore, it is concluded that food manufacturing companies should continuously invest in and focus on ESG management to secure a competitive advantage in the market and achieve sustainable growth.