The objective of this study was to evaluate the sperm liquid storage diluted with Brine Mineral Water (BMW) in miniature pig. Therefore we performed to find optimal concentration of BMW. The ejaculated semen from miniature pig was collected by gloved-hand method. The collected semen was diluted with dilution solution (Mulberry Ⅲ; modified-Modena B) which BMW was added. Concentration of BMW was 0, 2.5, 5, 7.5, 10 and 12.5% in dilution solution. The diluted semen was preserved at 17℃. Sperm ability was estimated by viability, capacitation acrosome reaction using chlortetracycline (CTC), hypoosmotic swelling test (HOST), morphologic abnormality. The diluted semen was observed for 7 days. The viability was significantly measured higher at 2.5% concentration of BMW than other groups (p<0.05). The AR-pattern of CTC analysis was significantly measured lower at 12.5% concentration of BMW than other groups (p<0.05). However, abnormality was not significantly different among all the groups (p<0.05). In conclusion, viability of sperm was the highest at 2.5% concentration of BMW but BMW had a negative effect on HOST, capacitation and acrosome reaction in sperm of miniature pig.

In this study, in order to analyze the air quality of the indoor environments of schools, we measured the indoor, outdoor and personal exposure concentration level of PM10 for 40 classrooms(20 old, 20 new) in chungnam area from June 22 to July 19 and from November 21 to December 30, 2003. 1. Old classrooms contained more dust than new classrooms; the average of respirable dust is 43.27 ㎍/㎥ for new classrooms while 53.38 ㎍/㎥ for old one. The exposure concentration level of respirable dust in new classrooms were in summer higher outdoors than indoors. The values were indoors 46.71 ㎍/㎥, outdoors 50.46 ㎍/㎥, and personal 41.62 ㎍/㎥. Meanwhile in winter indoors had a higher concentration level than outdoors, the values being indoors 39.11 ㎍/㎥, outdoors 34.86 ㎍/㎥, and personal 49.01 ㎍/㎥. 2. Cr concentration level within dust was slightly higher in summer indoors (101.50±32.10 ng/㎥) and outdoors (100.89±35.18 ng/㎥) than winter indoors (85.80±48.95 ng/㎥) and outdoors (74.43±38.93 ng/㎥), but in personal concentration level, winter was higher. The results of this research show insufficient understanding of health risks from indoor air pollution, and shows possible health problems to students from school indoor air pollution. As such, a logical and systematic education program for students about the importance of indoor air quality should be carried out. Also the results of PM10 concentration level measurements emphasize the need for regular measurements of indoor / outdoor and personal concentration level. New classrooms in particular needs to be used after measuring pollutants and safety, and requires installation of a ventilation device in all classrooms to improve air quality.