기후변화로 인해 국내에서의 기온, 강수량, 호우일수가 증가 할 것으로 예측됨에 따라, 토양 답압과 높은 불투수면적 비율이 나타나는 도심지를 중심으로 정원과 공공녹지에 활용되는 식물 소재에 있어 내침수성이 중요한 특성으로 부각될 가능성이 높 다. 그러나 내침수성 식물소재에 대한 대다수의 문헌은 재배경 험이나 각 종의 서식지 환경에 기반하고 있는 경우가 많아 실험 적 검증이 필요하다. 따라서 본 연구는 토양수분함량이 높은 서식지에서 기원한 골등골나물(Eupatorium lindleyanum DC.), 새등골나물(E. fortunei Turcz.), 좀개미취(Aster maackii Regel) 3개 종과 중간인 서식지에서 기원한 까실쑥부쟁이 (A. ageratoides Turcz.) 1개 종의 침수조건에 대한 생육반응 비교로 서식지 환경이 내침수성 평가의 유효한 지표인지 알아보 고자 하였다. 실험대상 종을 6주간 무처리(대조구), 5일 침수처리, 7일 침수처리한 결과, 골등골나물과 새등골나물은 대조구와 처리구간 생육반응에서 유의한 차이가 관찰되지 않아 높은 수준 의 내침수성을 지니는 것으로 나타났다. 좀개미취는 생장반응이 처리구에서 대조구 대비 유의하게 감소하여 내침수성은 제한적 인 것으로 보였으나, 5일 침수처리구에서 광계 II의 최대양자효 율(Fv/Fm)이, 두 처리구 모두에서 지상부 대비 지하부의 비율 (R/S율)이 대조구와 유의한 차이를 보이지 않아 침수조건의 해 소 후 회복 가능성을 지니는 것으로 나타났다. 까실쑥부쟁이는 처리 4 주차에 모든 처리구에서 100%의 고사율을 보여 내침수 성을 갖추지 못한 것으로 판단되었다. 결론적으로, 서식지 환경 은 내침수성과 관련이 있다 할 수 있었으나, 내침수성의 정확한 평가를 위해서는 토양수분함량 외 다양한 환경요소들을 함께 고려해야할 필요성이 있을 것으로 보인다.

본 연구는 국내에서 실내 관상용으로 많이 활용되는 스킨답 서스의 실내재배 시 적정 인공광원, 수경재배 전기전도도 농 도 및 용토를 선발하기 위하여 수행하였다. 실내재배 시 인공 광원은 형광등, 적청 LED, 백색 LED로 처리하였다. 수경재배 시 양액 농도는 EC 1.2, 1.6, 2.0ds·m-1 수준으로 하였고, 재 배용토는 제올라이트, 코코피트, 펄라이트, 황토볼 4가지로 달리하였다. 스킨답서스의 실내 재배 시 줄기 길이는 형광등 과 적청 LED 처리보다 백색 LED 처리에서 길어졌다. 잎의 크기는 적청 LED 처리에서 형광등과 백색 LED 처리보다 커 지는 경향이었다. 엽록소 지수값은 백색 LED>적청 LED>형광 등 순으로 높았다. 수경재배 시 스킨답서스의 줄기길이는 EC 농도가 높아질수록 길어지는 경향이었고, 용토별로 비교하면 제올라이트와 코코피트에서 높은 경향이었다. 엽록소 지수값 도 EC 농도와 비례하여 높아졌고, 용토별로는 제올라이트와 코코피트에서 높았다. 스킨답서스의 생체중도 EC 농도가 높 을수록 높았고, 제올라이트와 코코피트에 높은 경향이었다. 상기 결과들을 종합할 때, 스킨답서스의 NFT를 이용한 실내 재배 시 적정 인공광원으로는 백색 LED를, 수경재배 양액의 농도는 EC 1.6∼2.0ds·m-1를, 그리고 NFT 용토는 제올라이 트나 코코피트가 적합하다는 것을 알 수 있었다.

Intermuscular fat is essential for enhancing the flavor and texture of cultured meat. Mesenchymal stem cells derived from intermuscular adipose tissues are a source of intermuscular fat. Therefore, as a step towards developing a platform to derive intermuscular fat from mesenchymal stem cells (MSCs) for insertion between myofibrils in cultured beef, an advanced protocol of intermuscular adipose tissue dissociation effective to the isolation of MSCs from intermuscular adipose tissues was developed in cattle. To accomplish this, physical steps were added to the enzymatic dissociation of intermuscular adipose tissues, and the MSCs were established from primary cells dissociated with physical step-free and step-added enzymatic dissociation protocols. The application of a physical step (intensive shaking up) at 5 minutes intervals during enzymatic dissociation resulted in the greatest number of primary cells derived from intermuscular adipose tissues, showed effective formation of colony forming units-fibroblasts (CFU-Fs) from the retrieved primary cells, and generated MSCs with no increase in doubling time. Thus, this protocol will contribute to the stable supply of good quality adipose-derived mesenchymal stem cells (ADMSCs) as a fat source for the production of marbled cultured beef.

We aimed to predict the Italian ryegrass (IRG) productivity change of introduced and domestic varieties based on climate factors and identify suitable areas for IRG cultivation using the RCP 8.5 scenario. The minimum mean air temperature in January showed the highest correlation with productivity. The ratio of possible and low productivity areas was high in Gangwondo, and the ratio of suitable and best suitable areas was relatively high in the central and southern regions in the past 30 years. The change in the IRG cultivation area was found to be 26.9% in the best suitable area between 1981–2010 but increased significantly to 88.9% between 2090s. In the IRG suitability comparison classes between domestic and introduced cultivars, the ratio of suitable and best suitable areas was relatively high in the domestic varieties during the past 30 years. However, there is almost no difference between the IRG domestic and introduced varieties in the IRG suitability classes after the 2050s. These results can predict changes in the IRG suitability classes between domestic and introduced cultivars according to the climate change scenario, but there are limitations in accurately predicting the productivity of IRG because the results may vary depending on other environmental factors.

Mesenchymal stem cells (MSCs) are capable of differentiating into mesenchymal tissue such as bone, cartilage, muscle, and adipose, and have been isolated and characterized from various species. Deer adipose tissue-derived MSCs (dAD-MSCs) have not been studied and deer bone marrow-derived MSCs (dBM-MSCs) have not been fully characterized. In this study, we firstly isolated MSCs from deer tissues and then compared characteristics of dAD-MSCs and dBM-MSCs. dAD-MSCs and dBM-MSCs exhibited significant increase in proliferation under low-glucose DMEM culture condition during 20 and 10 passages consecutive passages, respectively. Both cells expressed cell surface markers such as CD73, CD90, and CD105, but did not express CD34 and CD45. Two types of cells expressed stemness markers (Oct4, Sox2, and Nanog) and exhibited differentiation potential into mesodermal lineages. Both cells exhibited osteogenic and chondrogenic differentiation potential, but poor adipogenic differentiation potential. Specifically, dAD-MSCs have a greater capacity for chondrogenic differentiation potential compared to dBM-MSCs. Collectively, we successfully isolated dAD-MSCs from deer for the first time. This study suggests that adipose tissue of deer could be used as a source of deer MSCs.

Coke aggregates and carbon artifacts were produced to investigate the interactions of coke and pitch during the kneading process. In addition, the kneading ratio of the coke and binder pitch for the coke aggregates was controlled to identify the formation of voids and pores during carbonization at 900°C. Experiments and thermogravimetric analysis revealed that carbon yields were improved over the theoretical yield calculated by the weight loss of the coke and binder pitch; the improvement was due to the binding interactions between the coke particles and binder pitch by the kneading process. The true, apparent, and bulk densities fluctuated according to the kneading ratio. This study confirmed that an excessive or insufficient kneading ratio decreases the density with degradation of the packing characteristics. The porosity analysis indicated that formation of voids and pores by the binder pitch increased the porosity after carbonization. Image analysis confirmed that the kneading ratio affected the formation of the coke domains and the voids and pores, which revealed the relations among the carbon yields, density, and porosity.