집단 내 갈등연구는 갈등의 유형을 과업갈등과 관계갈등으로 분리하여 집단성과에 미치는 서로 다른 영향에 초점을 맞추었지만, 대다수의 선행연구는 관계갈등뿐만 아니라 과업갈등도 집단성과에 부(-)적인 영향을 미치는 것으로 보고하고 있다. 또한 과업갈등과 관계갈등 사이에서 관찰되는 밀접한 정(+)의 상관 관계로 두 갈등 유형간의 전이효과에 대한 연구의 필요성이 제기되고 있다. 본 연구의 목적은 크게 두 가지로 나눌 수 있다. 첫째, 집단 내 갈등유형 간의 전이과정을 약화시킬 수 있는 집단 내 전반적 공정성 풍토의 조절역할을 살펴보고, 둘째, 집단 구성원들이 지각하는 전반적 공정성 풍토가 과업갈등이 집단성과에 긍정적인 영향을 이끌어 낼 수 있는 하나의 상황 조건인지를 검증하였다. 이를 위해, 83개 작업 집단을 대상으로 설문 자료를 수집하였다. 조절회귀분석 결과, 관계갈등과 과업갈등 간의 관계에 전반적 공정성 풍토의 조절효과가 유의미하게 나타났다. 전반적 공정성 풍토가 낮을 때, 관계갈등과 과업갈등 사이에 정(+)의 상관관계가 강하게 나타났지만, 전반적 공정성 풍토가 높을 때, 두 갈등 유형간의 상관관계는 많이 약화되었다. 또한 과업갈등과 집단성과 간의 관계에 대한 전반적 공정성 풍토의 조절효과도 유의미하게 나타났다. 전반적 공정성 풍토가 낮게 지각 될 때, 과업갈등은 집단성과에 부(-)의 효과가 강하게 나타났지만, 전반적 공정성 풍토가 높게 지각될 때, 집단성과에 대한 과업갈등의 부(-)의 효과는 더 이상 나타나지 않았다

In this paper, we present the result of investigations pertaining to the elastic buckling of simply supported columns with various cross-sectional dimensions but the same length and volume. In the investigations the accuracy of the analysis methods is studied and it was found that the result obtained by the successive approximations technique is the most accurate. In addition, the elastic buckling loads of columns with variable cross-section dimensions are obtained by the theoretical and numerical methods. From the results, it was found that the buckling loads obtained by the numerical methods are close to the buckling loads obtained by the successive approximations technique for the practical standpoints. Moreover, the buckling load of column with convexity in its middle is the highest while the buckling load of the tapered column is the lowest as expected.

This study was carried out in the peatland for estimating the paleoenvironment based on the analysis from its fossils plant and radiocarbon at the Hwayang-ri, Hyeondeok-myeon, Pyeongtaek-city, South Korea. Variety of fossil was collected from the peatland which could discriminated with naked eye and sorted into seeds, leaves, stems from four-stage of standard sieve. During analysis for the large plant fossil within the peatland, the dead plants were largely divided into 3 fossil zones according to its peatland. In the fossil zone Ⅲ(the oldest layer; 6,970~6,070 yr C14 B.P.), the observation of leaves of hydrophyte such as Trapa sp. and stems of Alnus japonica which appeared in wetland means that the environment was influenced by the climate changes such as rainfall increasing and sea level rise. In the fossil zone Ⅱ(the middle layer; 6,070~5,800 yr C14 B.P.), the occurrence of Gramineae, Cyperaceae and Fagaceae indicate that the environmental condition might be more or less dry by decreasing rainfall and drop in sea level. The fossil zone Ⅰ(the recent layer; 5,800~4,540 yr C14 B.P.) where lots of herbaceous plants, increasing of Fagaceae, decreasing of A. japonica. and distribution of Pinaceae were showed, was inferred to be repeated both dry and wet environment due to human disturbance.

Alfalfa (Medicago sativa L.) is one of the most important forage legumes in the world. It has been demanded to establish the efficient transformation system in commercial varieties of alfalfa for forage molecular breeding and production of varieties possessing new characteristics. To approach this, genetic transformation techniques have been developed and modified. This work was performed to establish conditions for effective transformation of commercial alfalfa cultivars, Xinjiang Daye, ABT405, Vernal, Wintergreen and Alfagraze. GUS gene was used as a transgene and cotyledon and hypocotyl as a source of explants. Transformation efficiencies differed from 0 to 7.9% among alfalfa cultivars. Highest transformation efficiencies were observed in the cultivar Xinjiang Daye. The integration and expression of the transgenes in the transformed alfalfa plants was confirmed by polymerase chain reaction (PCR) and histochemical GUS assay. These data demonstrate highly efficient Agrobacterium transformation of diverse alfalfa cultivars Xinjiang Daye, which enables routine production of transgenic alfalfa plants.

This study was conducted to investigate the effects of bacterial inoculation (Lactobacillus plantarum) and cutting height on the chemical composition, fermentation characteristics and in vitro dry matter digestibility (IVDMD) in whole crop barley silage. Barley forage (Youngyang hybrid) was harvested at about 27% of dry matter (DM) level at two different cutting height (5 vs. 15 cm). And it was chopped to 5 cm length and treated with or without L. plantarum. Four replicates of each treatment were ensiled into 10 L mini silo (3 kg) for 100 days. After 100 days, bacterial inoculation decreased (p=0.001) DM content, while increased cutting height increased (p=0.002) DM in uninoculated silage. Crude protein (CP) concentration was decreased by increasing height in uninoculated silage (8.84 vs. 8.16) but increased in inoculated silage (8.19 vs. 8.99). Both neutral detergent fiber (NDF) (p<0.011) and acid detergent fiber (ADF) (p<0.004) were decreased by increasing cutting height of forage at harvest. The IVDMD and ammonia-N was increased (p=0.001) by increasing cutting height and inoculation, respectively. Lactic acid bacteria (LAB) was increased (p=0.002) in inoculated silage, but yeast count was decreased (p=0.026) in uninoculated silages. It is concluded that increased cutting height of forage at harvest could be useful to make a fibrous portion with increase of dry matter digestibility of silages.

다변량통계기법은 수리지구화학 자료의 분석 및 해석에 많이 이용되어 왔다. 본 연구에서 대응분석과 주성분분석을 동시에 사용하여 인위적인 활동에 의한 지하수의 특징을 살펴보았다. 본 연구의 목적은 NETPATH 프로그램 속의 WATEQ4F를 이용하여 지하수 화학성분의 분화를 계산하고 이를 다변량통계기법을 이용하여 지구화학적인 정보를 추출하는 것이다. 연구지역은 한반도의 남동쪽에 위치한 울산의 LPG 저장시설이다. 본 연구지역에서는 다른 저장시설에서 관찰되는 초염기성의 조성을 가지는 지하수가 관찰되었다. 이러한 인위적인 영향에 의한 높은 pH를 가지는 지하수로 인해 Al의 분화특징과 탄산염의 침전을 유발할 수 있다. 본 연구에서는 연구지역에 지하수에 영향을 주는 두 인위적인 요소(세정작용와 시멘트영향)에 의해서 수리지구화학적인 특징과 상이 어떻게 변하는 가에 초점을 두었다. 이전 연구결과와 두 통계분석을 통해 제시된 결과를 비교하여 지구화학적인 정보를 이용한 주성분분석과 대응분석인 수리지구화학 연구에서 기초연구로 활용될 수 있음을 알 수 있다.

The objective of this study was to evaluate the characteristics of Korean Native Cattle sperm frozen-thawed with L-cysteine and/or catalase. The semen from bulls was collected by the artificial vagina method, and Triladyl containing 20% egg-yolk and/or L-cysteine (L), catalase (C) and L-cysteine + catalase was added to the diluted semen for cryopreservation. The results showed that sperm viability was significantly higher in the L-cysteine + catalase (69.49 ± 3.16%) group than in the control (60.5 ± 3.94%) group (p<0.05). Acrosome damage was significantly lower in the L-cysteine (17.12 ± 1.08%) group than in the control (21.46 ± 1.14%), catalase (20.54 ± 0.76%), and L-cysteine + catalase (19.29 ± 0.65%) groups (p<0.05). In addition, the level of intact mitochondria in the spermatozoa was significantly higher in the L-cysteine (58.65 ± 1.39%) group than in the control (50.63 ± 2.37%) group (p<0.05). The hydrogen peroxide level in the frozen-thawed sperm was significantly lower in the L-cysteine (3.74 ± 1.66%), catalase (4.65 ± 1.87%), and L-cysteine + catalase (8.11 ± 2.15%) groups than in the control (13.22 ± 1.6%) group (p<0.05). The glutathione level was significantly higher in the L-cysteine (1.33 ± 0.03%) group than in the control (1.08 ± 0.06 %), catalase (1.05 ± 0.02%) and L-cysteine + catalase (1.11 ± 0.03%) groups (p<0.05). In conclusion, L-cysteine and catalase could protect the membrane of Korean Native Cattle sperm from damage during sperm cryopreservation. Especially, L-cysteine was more effective for keeping acrosomes and mitochondria intactness during sperm cryopreservation.

Chios Gum Mastic (CGM) is a natural resin extracted from the leaves of Pistacia lentiscus, a plant endemic to the Greek island of Chios. It has been used by traditional healers, and it has antibacterial, antifungal properties, and therapeutic benefits for the skin. The CGM reduces the formation of dental plaque and bacterial growth in oral saliva, and recent studies have demonstrated the role of antioxidant activity of CGM. Although CGM has been widely investigated, its protective effect against oxidative-damage to keratinocytes, as well as the relationship between CGM and autophagy, has not been investigated. The aim of this study was to assess the protective effect of CGM against H2O2-induced oxidative stress and to evaluate the autophagic features induced by CGM in human keratinocytes. The pretreatment with CGM significantly reduced apoptosis in H2O2-exposed HaCaT cells. It promoted the degradation of caspase-3, caspase-8, and caspase-9; and it induced the formation of the processed PARP. The treatment with CGM caused an increase in vesicle formation compared to control group. The level of p62 was reduced and the conversion of LC3-I to LC3-II was increased in CGM treated HaCaT cells. Also, the treatment with CGM increased cleavage of ATG5-ATG12 complex. In summary, CGM helps the cells to survive under stressful conditions by preventing apoptosis and enhancing autophagy. Besides, the present investigation provides evidence to support the antioxidant potential of CGM in vitro and opens up a new horizon for future experiments.

한국산 동백나무류에서 서식하는 3종의 국내 미기록 혹응애(전기문목: 혹응애과), Acaphylla theae (Watt), A. theavagrans Kadono 및 Calacarus carinatus (Green)에 대한 분류학적 설명과 주요형질의 삽화를 제공하고, 기주식물과 피해양상 등의 생물학적 정보 또한 제공한다.

Activated carbon nanofibers(ACNF) were prepared from polyacrylonitrile (PAN)-based nanofibersusing CO2 activation methods with varying activation process times. The surface and structural characteristics of the ACNF were observed by scanning electron microscopy and X-ray diffraction, respectively. N2 adsorption isotherm characteristics at 77 K were con-firmedby Brunauer-Emmett-Teller and Dubinin-Radushkevich equations. As experimental results, many holes or cavernous structures were found on the fibersurfaces after the CO2 activation as confirmedby scanning electron microscopy analysis. Specificsurface areas and pore volumes of the prepared ACNFs were enhanced within a range of 10 to 30 min of acti-vation times. Performance of the porous PAN-based nanofibersas an electrode for electrical double layer capacitors was evaluated in terms of the activation conditions.

In this work, nanocomposites of epoxy resin and chemically reduced graphene oxide (RGO) were prepared by thermal curing process. X-ray diffractions confirmed the microstructural properties of RGO. Differential scanning calorimetry was used to evaluate the curing behaviors of RGO/epoxy nanocomposites with different RGO loading amounts. We investigated the effect of RGO loading amounts on the mechanical properties of the epoxy nanocomposites. It was found that the presence of RGO improved both flexural strength and modulus of the epoxy nanocomposites till the RGO loading reached 0.4 wt%, and then decreased. The optimum loading achieved about 24.5 and 25.7% improvements, respectively, compared to the neat-epoxy composites. The observed mechanical reinforcement might be an enhancement of mechanical interlocking between the epoxy matrix and RGO due to the unique planar structures.

Multi-walled carbon nanotube (MWCNT)/epoxy composites are prepared by a vacuum assisted resin transfer molding (VARTM) method. The mechanical properties, fracture surface morphologies, and thermal stabilities of these nanocomposites are evaluated for epoxy resins with various amounts of MWCNTs. Composites consisting of different amounts of MWCNTs displayed an increase of the work of adhesion between the MWCNTs and the matrix, which improved both the tensile and impact strengths of the composites. The tensile and impact strengths of the MWCNT/epoxy composite improved by 59 and 562% with 0.3 phr of MWCNTs, respectively, compared to the epoxy composite without MWCNTs. Thermal stability of the 0.3 phr MWCNT/epoxy composite increased compared to other epoxy composites with MWCNTs. The enhancement of the mechanical and thermal properties of the MWCNT/epoxy nanocomposites is attributed to improved dispersibility and strong interfacial interaction between the MWCNTs and the epoxy in the composites prepared by VARTM.

2000년도 이후로 많은 분자 연구 논문들이 육각강에서 진행되었으며, 그 결과 방대한 양의 미토콘드리아 유전자 염기서열이 생산되었다. 본 연구에서는 2000년도부터 2009년까지 육각강에 보고된 COI 염기서열과 이들을 활용한 분자 연구 논문들을 분석하기 위하여, 58,323개의 COI 염기서열들을 바탕으로 보고된 488개의 분자 연구 논문들을 26개 목들과 사용된 COI 염기서열들의 5', 3', 중간 위치에 따라 재분류하였다. 세 지역의 COI 염기서열을 이용한 연구 논문의 수는 26개 목들에 따라 매우 다양하였다. 하지만, 7개 목들은 특정 지역의 COI 염기서열들이 선호되었음을 나타내었다. 예를 들어, 파리목과 메뚜기목에서는 5' 지역의 COI 염기서열을 사용하는 논문의 수가 가장 높았으며, 이에 반해 딱정벌레, 이목, 잠자리목, 더듬이벌레목, 대벌레목에서는 3' 지역의 COI 염기서열을 사용하는 논문의 수가 가장 높았다. 2000년 이전에 보고된 84개의 분자 연구 논문들과의 비교를 통해, 2000년부터 2009년까지 딱정벌레목, 파리목, 이목, 대벌레목에서는 1999년 이전에 각 목들에서 주로 사용되었던 COI 염기서열의 특정 지역과 같은 지역을 사용하는 경향성을 보여주었다. 본 연구는 육각강에서 2000년에서 2009년까지 보고되었던 분자 연구 논문 뿐만 아니라 이들 논문에서 사용된 COI 염기서열에 대한 전체적인 경향을 이해하는데 유용한 정보를 제공한다.

환경변화에 대응하여 정부 조직이 시민과 공공서비스에 더욱 집중하기 시작했다. 하지만 대부분의 정부 조직은 영리 기업에 비해 소비자친화성을 높이는데 있어 어려움이 많다. 정부가 제공하는 공공서비스의 품질을 향상시키기 위해 이 연구는 소비자 지표에 근거하여 서비스 프로세스를 향상시키는 모델링방법을 제안하였다. 이 모델링 방법은 시민 패널로부터 마케팅 설문 자료를 수집하고 여러 가지 지표를 계산하여 핵심 프로세스를 도출하며 고객 유지 및 성숙 전략을 고안하는

The core aim of this paper is to empirically scrutinize a strength characteristic and ductility of the beam-column frame of reinforced with steel subjected to the cyclic lateral load. First and foremost, I the author embarks upon making four prototypes vis-à-vis this research. Through this endeavour, the author has analysed cyclic behavior, fracture shape, ductility and energy dissipation of the normal beam-column frame and a beam-column frame of reinforced with steel. In addition, the survey has revealed the exact stress transfer path and the destructive mechanism in order to how much a beam-column frame of reinforced with steel has resistance to earthquake regarding all types of building, as well as school construction. To get the correct data, the author has compared the normal beam-column frame and three types of the beam-column frame of reinforced with steel following these works, the characteristic of cyclic behavior, destructive mechanism, ductility, and Energy dissipation of normal beam-column frame and a beam-column frame of reinforced with steel have been examined clearly.

The core aim of this dissertation is to empirically scrutinize a strength characteristic of beam-column frame subjected to the cyclic lateral load, a beam-column frame of un-reinforced masonry wall, and a shear wall frame. First and foremost, I embark upon making three prototypes vis-à-vis this research. By conducting this process, I touch on an analysis of cyclic behavior and a damage characteristic of the beam-column frame, the beam-column frame of un-reinforced masonry wall, and the shear wall frame. What is more, through the previous procedure, the next part delves into the exact stress transfer path and the destructive mechanism to examine how much and how strong the beam-column frame of un-reinforced Masonry Wall does have a resistance capacity against earthquake in all the architecture constructed by the above-mentioned frame, as well as school buildings. In addition to the three prototypes, two more experimental models, a beam-column frame and shear wall frame, are used to compare with the beam-column frame of un-reinforced masonry wall. Lastly, the dissertation will suggest some solutions to improve the resistance capacity against earthquake regarding all constructions built with non bearing wall following having examining precisely all the analysis with regard to not only behavior properties and the damage mechanism of the beam-column frame and the beam-column frame of un-reinforced Masonry Wall but also the resistance capacity against earthquake of non bearing wall and school buildings.

한반도에서 산출되는 초염기성 맨틀포획암의 지화학적 특징과 평형 온도와 압력 조건을 계산하고, 산소동위원소비를 분석하였다. 연구 결과 (1) 한반도 맨틀포획암은 전형적인 초염기성 포획암(MgO: 49.12-50.95 wt.%, Mg값: 90.1-92.2)으로 구성되어 있다. (2) 한반도 맨틀포획암의 평형온도는 854-1016˚C이고, 압력은 4.6-24.4 kbar로 얻어졌다. (3) 맨틀포획암을 구성하는 감람석의 산소동위원소비(δ18Ool)는 5.06-5.51‰의 균질한 값으로 N-MORB와 상부 맨틀 감람석의 값(δ18O: 5.2±0.2‰)과 유사하다. 그러나 백두산과 제주도의 맨틀포획암을 구성하는 감람석의 산소동위원비는 각각 5.07-5.51‰과 5.07-5.45‰로 상대적으로 넓은 범위의 δ18O 값을 갖고 있다. 이 결과를 바탕으로, 이 연구에서는 백두산 맨틀포획암의 높은 δ18O가 맨틀포획암 물질에 재순환된 퇴적물원 EM2 물질의 혼입 때문일 수 있다는 가능성을 제안하였다.

The purpose of the present study was to investigate the effect of IFN- on prostaglandin synthesis, cyclooxygenase-2 (COX-2) gene expression in vitro and concentration of progesterone (P4) in endometrial cells. Epithelial and stromal cells cultured in vitro were isolated from bovine endometrium and stimulated with increasing doses of IFN- (0, 0.02, 0.2 and 2 ug/ml). Human chorionic gonadotropin (hCG, 1.5 IU/ml) was used as a positive control. Prostaglandin and levels in the culture media were analyzed by enzyme immunoassays and total RNA was extracted from the cells for RT-PCR. P4 concentrations of blood samples were assayed by chemiluminescent immuno assays system. In epithelial cells, COX-2 gene expression was increased in the presence of IFN- (p<0.05), but it was not significantly different in all groups of stromal cells except for 2 ug/ml IFN- group (p<0.05). Although IFN- did not affect and production in epithelial cells, it decreased and production significantly in stromal cells (p<0.05). In vivo experiment, blood concentration of P4 was significantly increased after addition of IFN- (1 ug/ml). The results indicate that PG production was mediated by COX-2 expression in stromal cells but it was not affected in epithelial cells and this suggest that treatment of IFN- could improve the implantation environment of uterine by maintenance of high P4 concentration.

Epigenetic modification including genome-wide DNA demethylation is essential for normal embryonic development. Insufficient demethylation of somatic cell genome may cause various anomalies and prenatal loss in the development of nuclear transfer embryos. Hence, the source of nuclear donor often affects later development of nuclear transfer (NT) embryos. In this study, appropriateness of porcine embryonic germ (EG) cells as karyoplasts for NT with respect to epigenetic modification was investigated. These cells follow methylation status of primordial germ cells from which they originated, so that they may contain less methylated genome than somatic cells. This may be advantageous to the development of NT embryos commonly known to be highly methylated. The rates of blastocyst development were similar among embryos from EG cell nuclear transfer (EGCNT), somatic cell nuclear transfer (SCNT), and intracytoplasmic sperm injection (ICSI) (16/62, 25.8% vs. 56/274, 20.4% vs. 16/74, 21.6%). Genomic DNA samples from EG cells (n=3), fetal fibroblasts (n=4) and blastocysts from EGCNT (n=8), SCNT (n=14) and ICSI (n=6) were isolated and treated with sodium bisulfite. The satellite region (GenBank Z75640) that involves nine selected CpG sites was amplified by PCR, and the rates of DNA methylation in each site were measured by pyrosequencing technique. The average methylation degrees of CpG sites in EG cells, fetal fibroblasts and blastocysts from EGCNT, SCNT and ICSI were 17.9, 37.7, 4.1, 9.8 and 8.9%, respectively. The genome of porcine EG cells were less methylated than that of somatic cells (p<0.05), and DNA demethylation occurred in embryos from both EGCNT (p<0.05) and SCNT (p<0.01). Interestingly, the degree of DNA methylation in EGCNT embryos was approximately one half of SCNT (p<0.01) and ICSI (p<0.05) embryos, while SCNT and ICSI embryos contained demethylated genome with similar degrees. The present study demonstrates that porcine EG cell nuclear transfer resulted in hypomethylation of DNA in cloned embryos yet leading normal preimplantation development. Further studies are needed to investigate whether such modification affects long-term survival of cloned embryos.

본 연구는 학교 건물의 구조적 성능 평가를 통한 내진보강에 대한 연구이다. 본 연구의 목적은 학교 건물의 구조적 성능평가를 통하여 안전성과 사용성을 고려한 구조보강방안을 비교분석하고 합리적 내진 보강안을 제시하여 보다 지진에 안전한 건축물을 유지하는데 도움 이 되고자 한다. 이 목적을 위해 기존의 학교건물을 연구 대상으로 선정하여 내진성능평가를 실시하고 내진 보강안을 제시하였다. 본 연구의 방법은 기존의 철근콘크리트 학교건물을 대상으로 1차 내진 성능평가와 2차 내진 성능평가를 실시하였다. 위와 같은 방법으로 내진성능평가를 하여 그 결과를 분석하고 내진보강방안을 제시(강재댐퍼, 탄소막대보강재)하였다. 제시된 내진 보강방안을 대상 건축물에 적용하여 내진보강 전, 후의 내진성능평가를 통해 종합적인 결과를 도출 하였다.