본 연구는 영유아교사의 근무환경과 이직의도 관계를 알아보고 감정노동이 이 두 변수를 조절하는지 확인하는 데 목적이 있다. 연구를 위해 영유아교사 199명을 대상으로 빈도분석, 기술통계분석, 상관분석을 실시하였으며, 인구통계학적 주요변인의 차이검증을 위해 t-검증 (t-test) 및 일원분산분석(one-way ANOVA)을 실시하였다. 또한 감정노동 조절효과 검증을 위해 위계적 회귀분석과 단순기울기 분석을 적용하였다. 분석 결과, 근무환경의 하위 변인들은 감정노동의 내면적 행동에는 정적 상관관계를, 이직의도에는 부적 상관관계를 보여주었다. 영 유아 교사들의 감정노동은 표면적 행동과 내면적 행동에 정적 상관관계로 나타났으며 감정노 동의 표면적 행동은 이직의도와 정적 상관관계가 있는 것으로 나타났다. 낮은 물리적 환경과 대인관계에 기반 한 감정노동은 이직의도를 유도하지만 반면 감정노동은 근무환경과 이직의 도 간의 조절요인으로 작용하였다. 근무환경의 개선방안으로 영유아교사의 업무에 대한 코칭 개입 방법은 궁극적으로 이들의 이직의도를 낮추는 효과적인 방안일 수 있다. 즉 정서적인 지 지를 통해 스트레스적인 근무 환경을 정서 안정적이고 긍정적인 환경으로 변경하고, 물리적으 로 교재․교구비 지원, 근로시간 보장, 처우 개선을 통해 영유아 교사들의 근무환경에 대한 만족도를 높이는 것이 요구된다. 본 연구는 궁극적으로 코칭기법을 중심으로 교사 심리 상담 과 같은 정서적 지원프로그램을 제공하여 그들의 이직의도를 낮추는 방안을 제언한다.

호접란은 세계적으로 분화뿐만 아니라 절화로도 판매되는 주 요 화훼작물이다. 상업적 종묘 대량생산은 조직배양 기술에 의 해 이루어지고 있으나 우리나라는 아직까지 균일하고 우수한 발근묘 생산 기술이 확립되지 않았다. 생육이 고르고 우수한 발근 배양묘를 생산하기 위한 적정 배양 신초수를 찾기 위해 용적 500mL 유리 배양병에 호접란 2품종(‘Lovely Angel’과 ‘UniVivace’)의 신초를 1, 4, 7, 10개씩 배양한 후 3개월에 지상부 및 지하부 초기 생육 특성을 조사한 결과, 7개의 신초를 배양하였을 때 신초의 생육이나 뿌리 유도 및 생육에 효과적이 었다. 또한, 배양병 재질이 발근묘 생육에 미치는 영향을 확인하 고자 용적이 500mL로 동일한 유리 배양병과 플라스틱 배양병 에 신초 7개 배양 3개월 후 2품종의 생육 특성을 비교한 결과, 2품종 모두 플라스틱 배양병 보다는 유리 배양병이 유의적으로 생육에 효과적인 것으로 확인되었다. 기내 발근묘 생산을 위한 배양병 재질의 영향은 이후까지 지속적으로 영향을 미치는 것으 로 확인되었다. 결론적으로, 균일한 호접란 발근묘 생산은 플라 스틱 재질보다는 유리병 재질의 배양병이 적합하며, 특히, 용적 이 500mL인 유리 배양병의 경우 신초를 7개(묘당 재식면적 5.4㎠) 이내로 배양하는 것이 바람직한 것으로 확인되었다.

This study evaluated the virucidal efficacy of a chlorine dioxide (ClO2) gas-generating fumigation disinfectant consisting of sodium chlorate solution (25% sodium chlorate) and reaction solution against avian influenza virus (AIV). After AIV suspensions had been deposited on stainless steel carriers, the 9 dried carriers were exposed to the fumigant (sodium chlorate solution: 8.5, 17, 34, 50, and 100 mL) in a 25-m³ test room for 2, 3, and 4 h, respectively. Thereafter, all carriers were submerged in a neutralizing solution (20% fetal bovine serum) to scrape off the surviving viruses, and the respective suspensions were diluted. Each diluent was inoculated into the allantoic membrane of five 10-day-old embryos. After incubation for 5 days at 37℃, AIV viability in the collected allantoic fluids was examined, and the egg infectious dose 50 (EID50) was calculated. When the carrier was exposed to ClO2 gas generated from reacting 34 mL of the fumigant for 3 h, the AIV titer reduced by more than 104.0 EID50/carrier compared to the control, which was exposed to the fumigant without inoculation of AIV suspension. In addition, the control was non-toxic to the embryos.

A combination of a series of epoxy coatings filled with octadecylamine (ODA)-modified graphene oxide (mGO) or commercial exfoliated graphite nanoplatelets (xGnP) was developed to boost the anticorrosion performances of mild steel substrates in acidic and NaCl aqueous solutions. The xGnP and mGO were applied successfully as fillers for the preparation of layer by layer (LBL) xGnP or mGO/epoxy coatings, respectively, which were coated on the clean steel surfaces to form LBLassembled layers. The LBL-assembled xGnP or mGO/epoxy coating-coated steel substrates exhibit excellent anticorrosion performances. The corrosion potentials (Ecorr) of xGnP-1/xGnP-2/3 and mGO-1/mGO-2/3 display at − 193 and − 150 mV, respectively, while Ecorr of the bare steel shows at − 871 mV of immersion in the 3.5 wt% NaCl solution. The most positive Ecorr values are obtained for xGnP-1/2/3 (− 117 mV) and mGO-1/2/3 (− 66 mV), showing the best anticorrosion performances compared to the bare steel (− 404 mV) in 17 wt% HCl solution.

수염풍뎅이(Polyphylla laticollis manchurica)는 과거에는 흔히 발견되었으나, 1970년대 이후 한반도 내 개체수 가 급격히 감소하여 2005년 환경부에 의해 멸종위기 야생생물 Ⅰ급으로 지정되었다. 또한 해당종의 분자생물학적 연구는 멸종위기종이라는 특성으로 인해 제한적으로 진행되었다. 그로 인해 NCBI 등 공공 데이터베이스에서 제공되는 서열정보들 또한 부족한 실정이다. 이 연구는 이러한 한계를 극복하고 수염풍뎅이의 유전적 특성을 규명하기 위해 생물정보학적 기술을 활용하여 전사체 분석을 진행하였다. Illumina HiSeq 2500 플랫폼을 사용하여 53,433,048개의 RNA reads를 얻었으며, Trinity와 TGICL을 이용한 De novo 어셈블리 분석을 통해 18,172개의 unigenes를 생성하였다. 생성된 unigenes는 GO, KOG, KEGG, PANM DB를 활용하여 annotation을 진행하였다. 그 결과, GO 분석에서는 ‘binding and catalytic activities’와 관련된 항목이 높은 발현을 보였으며, KOG 분석의 경우 ‘Cellular Processes and Signals’ 범주가 높은 비율을 나타내었다. KEGG 분석을 통해 2,118개의 unigenes가 metabolic 카테고리에 annotation된 것을 확인하였다. SSR 모티프 분석에서는 AT/AT (42.90%) 모티프, AAT/ATT (13.13%) 모티프 순으로 많이 나타나는 것을 확인하였다. 이 연구를 통해 분석한 결과 들을 이용하여 유전자원 및 종 정보를 실시간 제공 및 정보 공유가 가능하도록 Database 및 web-interface를 구축하 였으며, 이러한 자료들은 국내 멸종위기종인 수염풍뎅이의 고유한 유전적 특성을 발굴 및 확보할 수 있는 기반자 료로써 활용될 수 있을 것으로 사료된다.

Haemaphysalis longicornis는 사람과 동물에게 여러 심각한 병원체를 전달하는 주요 매개체로, 한반도에 널리 분포하고 있다. H. longicornis는 Rickettsia spp., Borrelia spp., Francisella spp., Coxiella spp., 그리고 중증열성혈소판 감소증후군 바이러스 (SFTS virus) 등을 매개하는 것으로 알려져 있다. 국내에 서식하는 H. longicornis의 미생물 군집과 관련된 연구는 많이 진행되지 않은 것으로 확인되었다. 이 연구는 한반도 내 다양한 지역에서 채집된 H. longicornis의 미생물군집 다양성을 지역별, 성장 단계 및 성별에 따라 분석하였다. 2019년 6월부터 7월까지 질병관리청 권역별기후변화매개체감시거점센터 16개 지역에서 채집한 H. longicornis의 16S rRNA 유전자 V3-V4 영역을 PCR로 증폭 후 Illumina MiSeq 플랫폼으로 시퀀싱하였다. Qiime2를 활용한 미생물 다양성 분석을 통해 총 46개의 샘플에서 1,754,418개의 non-chimeric reads를 얻었으며, 평균 126개 의 operating taxonmic unit (OTU) 을 식별하여 총 1,398개의 OTU를 확인하였다. 대부분의 지역에서 Coxiella spp.가 우점종으로 나타났으며, 특히 Coxiella endosymbiont는 가장 높은 우점도를 보이며, Coxiella burnetii와 계통 발생 학적으로 유사한 것으로 확인되었다. 이 연구를 통해 분석된 결과는 각 지역의 H. longicornis 미생물군집 데이터 베이스 구축에 활용되었으며, 이를 통해 지역별 미생물군집의 특이성을 식별할 수 있게 하였다. 이는 한반도의 H. longicornis에 의한 질병 전파 연구와 이를 통한 공중보건 개선에 기여할 것으로 기대된다.

갈색거저리 유충의 사료인 밀기울은 대부분 수입에 의존하고 있는데 일부 국가의 식량 수출 중단 조치 등에 따른 국제 곡물가격 상승으로 밀기울 가격은 인상되고, 식용곤충 판매가격이 하락하면서 생산비 절감을 위한 사료 개발이 요구되고 있다. 농업부산물 3종을 50% 이상 급이하게 되면 유충 생육이 저하되었기 때문에 본 연구는 적정한 배합비율을 선정하기 위해 첨가사료 20, 30% 함량으로 밀기울과 혼합하여 사료를 급이하였을 때 갈색거 저리 유충의 생육 특성과 영양성분 변화에 대해 밀기울만 제공한 대조구와 비교하였다. 부산물 A와 B, C를 각각 30% 함유한 처리구에서 갈색거저리 유충 무게는 대조구와 차이가 없음을 확인하였다. 먹이소화율은 부산물B를 20% 함유한 처리구가 80.5%로 대조구에 비해 높았고, 부산물C 30% 처리구에서 72.6%로 가장 낮았다. 갈색거저 리 유충의 생육일수 100일 기준으로 부산물B 30% 처리구에서 용화율이 76.1%로 대조구보다 1.6배 높았으며, 부산물A 20% 처리구는 29.2%로 용화율이 가장 낮았다. 갈색거저리 유충의 조단백질 함량은 부산물 C 30% 처리 구에서 대조구보다 10.3% 증가하여 아미노산 분석을 진행한 결과 sarcosine과 ornithine이 2.5배 이상 증가하였다. 이를 통해 부산물 B나 C를 30% 함유한 사료를 급이하였을 때 사육 원가를 절감하여 유충을 생산할 수 있을 것으로 사료된다.

The purpose of this study is to select various insect species for healing resources and develop a healing program in order to use insect as a healing agriculture. In this study, there are two kinds of breeding kit were developed, one for Gryllus bimaculatus and the other for Oxya chinensis sinuosa. Using these insect breeding kits, we conducted a survey of 60 children and the elderly. In the case of children, the results of the insect breeding satisfaction showed that 30.6% said that the sound of crickets was very good, and 11.1% said that it was good. In addition, the higher the child's awareness of insects, the higher the proportion of children who wanted to raise insects in the future. As a result of a survey of seniors, 45.2% do not like insects and 51.6% are not interested, meaning that most seniors are not very interested in insects. However, the emotions after breeding insects showed positive results, with 45.2% saying their personality became brighter, 48.4% reducing their anger, 48.4% relieving their irritation, 54.8% relieving loneliness, 58.1% feeling more responsible, and 51.6% developing intimacy.

In response to the expanding landscape of the biotechnology industry and the increasing demand for comprehensive drug development as well as the conduct of preclinical and clinical trials, there is a growing need for employment of diverse animal models, including both small and large animals. The focus of this study was on refining ex vivo culture techniques for bioluminescence imaging following administration of intradermal injections in large animals. To examine the feasibility of our approach, varying concentrations of the rFluc protein were administered to rats and live imaging was employed to validate the corresponding levels of expression. Subsequently, following administration of rFluc to mini-pigs, ex vivo analyses were performed on sample tissues to assess the levels of protein expression across different concentrations. In particular, optimal culturing conditions that facilitated the sustained expression of the protein in samples post-euthanasia were identified. Moreover, by employing small animal imaging devices, we were able to capture clear images of the sample plates, which provided evidence of the successful application of our experimental techniques. The findings from this research represent a significant effort toward refining bioluminescence imaging methods tailored for use with large animal models—an imperative facet of contemporary drug development and biomedical research.

Aligned with the recent shift in fruit consumption trends, there is a growing increase in the cultivation of these fruits. Thus, various types of small fruits, including blueberries, have recently been introduced and cultivated in Korea. According to the data, there has been a notable uptick in blueberry farming, in stark contrast to the decline in cultivation of black raspberries, black chokeberries, and blackcurrants. New varieties of blueberries continue to be introduced and cultivated, aligning with the recent consumption trends and are expected to be consistently cultivated in the future. Despite the decrease in the other berries, health benefits associated with all berries have ignited growing consumer interest, resulting in domestic and international market expansion. In conclusion, this research underscores the importance of identifying plant varieties that are well-suited to Korea's climatic conditions, validating efficient cultivation strategies, and establishing robust distribution networks to foster sustainable development in the berry industry.

This study evaluated the fungicidal efficacy of weakly acidic hypochlorous acid water (WAHW) against Microsporum canis (M. canis) and its therapeutic effect on M. canis-infected mouse skin. WAHW was produced by a WAHW generation module. A fungicidal efficacy test by the broth dilution method was used to determine the lowest effective concentration of the WAHW. The lowest effective concentration of WAHW was less than 10 ppm. For T-1, T-2, and T-3, 30 ppm of WAHW was applied to the infected skin once, twice, and three times a day, respectively, and for T-4, 50 ppm of WAHW was applied once a day. On the 3rd day after the initiation of treatment, skin scores in all of the WAHW-treated groups were significantly decreased compared to those in the positive control group (PC) (p<0.05), and there were no significant differences compared to the normal control (NC). The area of the infected skin in all of the WAHW-treated groups was significantly decreased compared to PC from the first day after the initiation of WAHW treatment (p<0.05). The results showed that WAHW had a fungicidal efficacy on M. canis at less than 10 ppm, and it was effective in improving skin symptoms when 30 ppm of WAHW was applied to the M. canis-infected area once a day for five days or 50 ppm of WAHW was applied once a day.

The striped fruit fly, Zeugodacus scutellata., is ubiquitous in agricultural areas, making it a critical pest to monitor and manage. It is necessary to develop a technique for rearing Z. scutellata in the laboratory to achieve mass production for biological control. The pumpkin is an important host plant for Z. scutellata; females lay their eggs on the tops of pumpkin flower buds, and larvae consume the stamens from top to bottom before dropping to the ground. Several types of diets are considered in the present study, including liquid, solid, and semisolid diets. Methyl benzoate, propionic acid, sodium benzoate, and citric acid were tested for their ability to suppress microbial contamination and improve the shelf life of diets. Fresh pumpkin, pumpkin flower, and pumpkin powder were used as primary ingredient. Reared adults were tested for their egg laying and survival abilities based on different diets. Approximately 86% of adults emerged from liquid and semisolid diets. Solid diets reduce pupae production and adult emergence. The diets with a total viability of less than 75% were discarded, which is considered as a reasonable threshold to determine whether a diet is nutritionally sufficient to rear the flies. Based on the results of the present study, we have developed a diet for mass rearing in laboratories, which may assist in providing a scientific basis for the effective control of Z. scutellata.

The striped fruit fly (SFF), Zeugodacus scutellata, is an agricultural pest species with a strong and rapid reproductive ability that can cause significant harm. To control the population of these kind of pests, the sterile insect technique (SIT) is being used as one of the effective methods. SIT involves the introduction of sexually transmitted factors that reduce the reproductive capacity of males. This study shows that knocking down the testis-specific serine/threonine protein kinase 1 (Zs-Tssk1) gene alters male fertility and male-initiated types of communication. Since Zs-Tssk1 influences the physiology of the testes, spermatogenesis is also affected, which in turn alters the lifespan of Zs-Tssk1 knock down group in comparison with the control. Based on these results, Zs-Tssk1 may be crucial in reproductive function, and its down-regulation may be helpful in controlling SFF through SIT.

Social marketing is a discipline promoting voluntary behaviour change in target audience to effect societal and individual benefits by applying commercial marketing strategies. Considering the UNFCCC mechanisms are established to foster voluntary cooperation of parties of the Paris Agreement (2015) in achieving their emission reduction targets and adaptation goals, potential for social marketing playing a critical role in contributing to the context seems endless. The Climate Technology Center and Network (CTCN), established in 2013, is an operational arm of the Technology Mechanism (TM) introduced by the Conference of the Parties (COP) under the United Nations Framework Convention on Climate Change. With the purpose of pursuing impactful mitigation and adaptation technology transfer based on global cooperation ontology, the CTCN supports technical assistance (TA) to developing countries on climate technology issues. Nevertheless, the participation rate of the domestic members in the CTCN TA project is low at 7 per cent. With this regard, little has known about what government support would benefit the Network members most, therefore, contributing to the global climate change issue. This research aims to respond to these calls. Three sequential studies outlining 4P strategy development were conducted. Study 1 conducted an online survey to identify pricing factors influencing participation of climate technology transfer business (RQ1). Study 2 tested four different types of government support program that will is most likely to encourage the Network member’s CTCN TA participation (RQ2). Study 3 pilot tested the most appropriate government support program, including a new communication strategy (RQ3). This research has taken climate technology experts’ perspectives in order to enhance the participation of 81 Korean Network members in climate technology projects. This paper contributes to applying the social marketing approach in expanding climate technology assistants globally, thereby purposefully bringing new theoretical and practical insights affecting the mitigation of carbon emissions and increasing the adaptation to climate-related disasters in developing countries.