The objective of this study was to empirically analyze the shipping method and trading intent of landscaping tree farms. The analysis used survey data on landscaping tree seedling farms. Crosstabs were utilized to analyze the shipping method and characteristics of landscaping trees, and a binary logistic regression to analyze the trading intent. The results were as follows: The most common shipping method used by landscaping tree farms was “agricultural forward contract,” followed by “delivery of goods by individuals to middlemen.” However, “direct dealing with the ordering body” was the most desired in the long run. Among the characteristics of farms desiring direct dealing with the ordering body, variables that showed statistical significance were “experience in producing landscaping trees,” “whether landscaping tree production is a full-time profession,” “awareness of retail prices of landscaping trees,” and “difficulty in finding market and price information related to shipping.” In particular, more experience in producing landscaping trees led to a lower probability of direct dealing. Moreover, farms specializing in landscaping trees, farms aware of retail prices, and farms that had difficulty determining market or price information were more likely to focus on direct dealing.

The Korean Ministry of Education, Science, and Technology has made strenuous efforts to improve the quality of English education in public sector. One such attempt is a long-term, intensive teacher training that began in 2007 to facilitate teachers' ability for teaching English in English (TEE). This initiative has expanded over the years such that the number of training institutions has increased to 22 in 2009 to serve growing numberof trainees. This development is not without problems as there is a lack ofconsistency in curriculum, course content, quality of instructors, and overseas training among these institutions. This study aims to check the current status of various teacher training programs and suggest ideas for enhancing the quality of the programs. For the purpose of the study, questionnaires were administered to 900 English teachers to have themevaluate the training programs they had participated in. The findings from 449 respondents showed that most teachers were satisfied with their training programs. These teachers felt that the training programs helped to promote both English skills and teaching skills. Yet, they expressed some concerns about unbalanced curriculum, lack of follow-up service, inefficiency ofoverseas training, etc.

The hemipteran whitefly Bemisia tabaci (Gennadius) is one of the most destructive pests damaging more than 600 agricultural crop species worldwide. The B and Q biotypes are most widely spread in Korea but they are not distinguishable based on morphological characters. Carboxylesterase 2 (Coe2) was determined to be 5.9 times more expressed in B biotype compared to Q biotype. Comparison of deduced amino acid sequences of Coe2 (595 a.a.) showed a total of 3.85% polymorphisms between B and Q types but no differences in major active sites. Quantitative real-time PCR revealed that both B and Q biotypes possess a single copy of coe2, suggesting that the overexpression of Coe2 in B biotype is likely due to overtranscription. To determine the putative role of Coe2 in insecticide tolerance, esterases were separated by native isoelectric focusing (IEF) and inhibited by various insecticides. The putative Coe2 band was apparently inhibited by pyrethroid and organophosphate insecticides, but not by imidacloprid. These findings suggest that overexpression of Coe2 confers chemical defense against pyrethroid and organophosphate insecticides, perhaps by sequestration.

The hemipteran whitefly Bemisia tabaci (Gennadius) is one of the most destructive pests damaging more than 600 agricultural crop species worldwide. The B and Q biotypes are most widely spread in Korea but they are not distinguishable based on morphological characters. In order to search for protein markers that can be employed for rapid and accurate diagnosis of biotypes, two-dimensional PAGE (2DE) in conjunction with mass spectroscopic analysis were conducted. Eleven biotype-specific spots were repeatedly identified during three repetitions of 2DE and analyzed by Q-TOF. One of the B type-specific protein spots was identified as carboxylesterase 2 (Coe2). The transcript level of coe2 was determined to be 6 times higher in B type than in Q type by quantitative real-time PCR. In addition, comparison of genomic DNA sequence of coe2 between B and Q types identified a biotype-specific intron, from which specific primer sets were designed. One-step PCR using these biotype-specific primers successfully distinguished the two biotypes in a high accuracy. Availability of the biotype-specific protein and DNA markers will greatly improve the detection of B. tabaci biotype in the field.

To search for hyper-variable genetic markers that can distinguish regional populations of head lice, we screened the inter simple sequence repeats (ISSR) based on the genome database of body louse, which is closely related conspecific species. An ISSR mining software, SciRoKo 3.4, was employed to excavate ISSR markers from the genome database under the MISA mode (≥ 60 bp repeats). Entire body louse genome (ca.100 Mb) was loaded to SciRoKo for ISSRs mining. A total of 5,336 ISSRs were obtained, and primers specific to individual ISSRs were designed by the Primer 3 and DesignPrimer 1.0 softwares. In order to prove the compatibility of body louse ISSRs to head lice, 31 PCR primers were randomly chosen out of a total of 613 pairs, and their appropriateness was tested by comparing the amplified PCR band patterns between body and head lice. Eleven primer pairs that resulted in poor or little amplification were excluded, and 20 primer pairs were further tested for three head louse populations (California, Panama and Chung-ju, Korea). Finally, nine primer pairs ensuring robust amplification of highly variable band patterns were selected to use for population genetic study of head lice.

With the increasing prevalence of e-learning courses in higher education, it is important to investigate how the courses are being run and examine students’ perceptions of e-learning courses to provide an optimal learning environment. An end-of-course, summative evaluation and log files in an e-learning system were used in order to describe the characteristics and students’ perceptions of three e-learning English courses in a university. The section of background information in the evaluation showed that the majority of the students taking the courses are seniors, majoring in humanities and social sciences. Factors were found to influence students’ satisfaction with the courses such as use of various multimedia, interactions between instructor and students, and among students, and instructors’ evaluation of students’ achievement. A post-hoc analysis showed evidence significant differences existed by course. These findings of the study suggest that quality assurance is an issue in setting and managing e-learning English courses, which are expected to grow rapidly in a near future due to students’ increasing need for e-learning.