To attenuate and control the spread of infectious disease, a body of research has been conducted to generate safe vaccines and to continue national-level surveillance. However, understanding on viability and persistence of avian influenza virus (AIV) in infected carcasses, and effective disposal approaches are still limited up to date. Here, using HA test and RT-PCR, we assessed active status of AIV and degradation of viral RNA in collected specimens at different sites and time points. First, AIV infectivity was recovered until day 2, and viral nucleic acids persisted to day 14 and 21 in inorganic and organic samples, respectively, in sealed vials incubated at room temperature. Second, AIV was totally inactivated in all examined specimens, and viral RNA was not detectable at all time points tested at least one month post-infection in AIV-inoculated carcasses buried directly in soil or fiber reinforced plastic (FRP) bin. Lastly, among different burial sites in South Korea, 6 out of 17 sampling sites in Jeonbuk province showed the presence of viral genetic materials, while the rest of the field samples displayed neither the presence of infective AIV nor detectable viral RNA. This study showed a linear relation between time and degradation degree of viral RNA in buried samples suggesting that burial disposal method is effective for the control or at least attenuation of spread of AI infection in infected animals although consistent monitoring is required to verify safety of disposal.

Luehdorfia puziloi (Erschoff, 1872) is a monophagous butterfly species that occurs only once during spring in a year and distributed nationwide in Korea. From 2012 to 2013 the adult emergence has been monitored to assess how the species responds to temperature rise in the country. Monitoring sites were located in four different provinces, two in the northern part of the country and the others the southern part. In each site a small emergence cage was set up with 60 to 70 pupae and a HOBO data logger to record the soil temperature of the site from February to April. The earliest record from literatures and specimen collection data was 28th of March in Geojae-gun, one of far south parts of the country, during the late 90’s. In 2013, the butterfly started emerging in a southern site, Namhae-gun, from 20th of March, and it was the earliest record so far. The emergence from all of the sites occurred on average 7.75 days faster in 2013 than 2012. The emergence patters during the two years clearly showed that the adult emergence reached to 50% emergence rate faster as the soil temperature rose.

아티초크는 가을에 정식하여 겨울철 월동 중에 화아분화가 이듬해 5월경에 수확이 가능하다. 본 시험은 아티초크 육묘 중 저온처리를 하여 생산시기를 7월로 늦추고자 수행하였다. 시험품종은 ‘그린글로브’ 및 ‘임페리얼스타’를 이용하였다. 파종은 72공 플러그 트레이를 이용하여 2월 10일에 파종하였고 4주간 17oC에서 육묘한 후 3, 6, 9, 12oC에서 4주간 육묘하였으며 4월 8일에 노지 포장에 정식하였다. 재식거리는 이랑폭 150cm에 주간거리 50cm(1,523주/10a), 1조식으로 하였다. 온도 처리별로는 6oC 처리에서 화뢰 발생이 63%로 가장 많았고 12oC에서는 33%로 가장 낮았으며 무처리의 경우는 5%의 화뢰가 형성되어 저온에 민감한 품종임을 알 수 있었다. ‘그린글로브’ 품종의 경우 9oC에서 화뢰 형성율이 가장 높아 28%였고 12oC에서는 10%로 가장 낮았으며 무처리의 경우는 화뢰가 전혀 형성되지 않았다. 화뢰중은 ‘임페리얼스타’ 품종이 97g 내외였고 ‘그린글로브’ 86g 내외로 자랐다. 수량은 ‘임페리얼스타’에서 215kg, ‘그린글로브’에서는 108kg을 보였다.

To produce abiotic stress resistant transgenic cucumber, the cotyledonary node explants of cucumber (c.v. Eunsung) were inoculated with A. tumefaciens strain EHA105 containing the binary vector (pPZP211) carrying Nit gene. The 491 explants inoculated with bacterium solution for 30 min were maintained on 50 mg/L paromomycin contained shoot induction (SI) medium for first 2 weeks and then subcultured on 100 mg/L paromomycin to obtain transgenic adventitious shoots for 4 x 14 days. So far, 5 plant were selected, and then acclimated in soil. Of them, 3 transgenic plants with Nit gene were confirmed by Southern blot analysis.

Soyasaponins A1 , DDMP-conjugated group B soyasaponins αg~;and~;βg , non-DDMP counterpart soyasaponin I, II+III, and DDMP moiety were quantified in the large-, midium-, and small-seed soybean varieties. Protein contents were ranged from 38.1% to 41.8%, and oil contents were ranged from 15.5% to 18.9%, respectively. Oil contents in the large-seed varieties were significantly higher than those of medium- and small-seed varieties. Among detected soyasaponin peaks, βg was a major soyasaponin in DDMP-conjugated group B soyasaponins followed by soyasaponin I, DDMP moiety and A1 . Soyasaponin concentration among different seed size soybean varieties. The soyasaponin concentration of mediumseed (4014.5~mug/g ) was slightly higher than those of largeseed (3755.0~mug/g ) and small-seed varieties (3620.3~mug/g ), however, the differences was statistically not significant. The composition rates of soyasaponins in the large-size seeds were 9.4% of soyasaponin A1 , 26.5% of DDMP-conjugated soyasaponins, 49.9% of non-DDMP counterpart soyasaponins, and 14.2% of DDMP moiety, respectively. Similar results were observed in the composition ratios of middle- and small-size seeds. Oil content and C:N ratio showed the significant positive correlations with total soyasaponin concentration, while the 100-seed weight, fiber, and ash contents showed the negative correlations with total soyasaponin but statistically not significant. It was noted that protein contents didn't have any relationship with group A, group B, DDMP moiety, and total soyasaponin. This fact suggested that protein contents are not affects the variation of soyasaponin concentration.