본 연구에서는 청강문화산업대의 여학생 중 체지방률이 30% 이상인 총 40명을 대상으로 다이어트 프로그램 S-28을 실시함에 있어서 1997년 4월 1일부터 8월 31일까지 5개월 동안의 장기적인 영양교육의 효과를 관찰한 결과이다. 처음 다이어트 2주 동안은 하루 800㎉, 다음 2주 동안은 1, 200㎉가 제공되었으며, 그후에도 제충유지 프로그램이 계속되었다. 운동과 영양교육 프로그램은 다이어트 전 기간을 통해 진행되었다. 실험결과를 요약하면 다음과 같다. 1) 체중은 초기 측정치 69.5±4.9㎏에서 3개월째 65.6±3.0㎏(P<0.01)으로 유의적인 감소를 하였으며, 5개월째에도 63.2±3.5㎏(P<0.01)으로 초기 체중에 비해 유의적인 감소를 하였다. 2) 비만도는 초기 23.9±12.1%에서 3개월째 21.3±9.2%(P<0.05), 5개월째 19.7±8.5%로 감소하였다. 3) BMI는 초기 27.3±1.5㎏/㎡에서 3개월째 26.1±1.2㎏/㎡(P<0.01)로 감소하여 유의적인 차이를 보였으며, 5개월째는 25.7±0.9㎏/㎡(P<0.01)로 감소하였다. 4) 체지방률은 다이어트 시작시 31.1±2.5%에서 3개월째는 29.0±1.3%(P<0.05), 5개월째는 28.2±1.8%(P<0.05)로 유의적으로 감소하였다. 체지방량은 다이어트 시작시 22.6±3.5㎏에서 3개월째 20.7±3.6㎏(P<0.05), 5개월째 20.1±2.9㎏(P<0.05)으로 유의적으로 감소하였다. 5) 식습관 점수는 초기 33.4±4.5점에서 3개월째 35.2±3.7점, 5개월재 37.4±4.0점으로 다소 증가하였으며, 영양태도 점수도 초기 53.8±5.3점에서 3개월째 55.7±5.3점, 5개월째 58.0±6.2점으로 증가하였다.

산업체 급식소에서 생산직 근로자에게 제공되고 있는 음식의 적정 분량을 제시하기 위해 목포지역 생산직 근로자 240명을 대상으로 섭취량 측정과 설문지를 이용한 만족도 평가를 실시하였다. 조사대상 근로자의 연령은 30∼39세가 57.1%, 학력은 대부분 고졸이었으며, 건강상태는 52.1%가 건강하다고 하였다. 근로자의 좋아하는 식사의 형태는 한식이었고, 식품을 선택할 때 가장 중요하게 생각하는 것은 맛으로 나타났으며, 식사의 가치관은 조사대상 근로자의 37.1%가 공복을 채우는 것이라고 대답해서 생리적 욕구충족에 가치를 둔 비율이 가장 높았다. 산업체 급식소에서 제공된 음식의 섭취량과 섭취율은 밥류 238g(98.4%), 국류 212g(82.7%), 찌개류 230g(77.2%), 볶음류 40g(80.7%), 조림류 60g(83.5%), 무침류 42g(72.3%), 김치류 51g(75.9%), 일품요리류 406g(77.7%), 구이류 51g(72.6), 부침류 48g(48.7%)였다. 섭취량에 대한 만족도 평가 결과, 섭취량에 대해서 두 비교군 거의 모두가 `적은 양`이라고 대답하였다. 섭취량에 대한 만족도 평가를 기초로 음식의 적정량에 대해 밥류 240∼270g, 국류 270g, 찌개류 310g, 볶음류 60g, 조림류 75g, 무침류 76g, 김치류 67g, 일품요리류 470g, 구이류 80g, 부침류 50g으로 제시할 수 있다.

Sweet potato β-amylase is a tetrameric enzyme consisting of four identical polypeptide chains with a molecular weight of 5.6×10 exp (4), though most of the other β-amylases are monomeric enzymes. But, the relationship between subunit structure and catalytic function of the enzyme is not known. This study was done to know what the function of the subunit structure of the enzyme is. We obtained the monomer from the enzyme by the treatment of SDS, alkali pH buffer and urea. But the monomer had not activity. We tried to prepare the active monomer from the enzyme by the modification with periodate-oxidized soluble starch. In the result, we succeeded in isolating an active monomer as an oxidized soluble starch-conjugated form. The active monomer had 57% of the original activity, 13.2% of the sugar and the molecular weight was estimated to be 6.4×10 exp (4). This results suggest that the tetrameric form of the enzyme is a most stable one and exists in nature, and the subunit structure of the enzyme plays an important role in stabilization but not catalytic function.

Background : The hyphal growth of Poria cocos are known to grow well under medium temperature(25 - 28 ℃) and acidic(pH 4.0 - 5.0) conditions. Also, it is known that a large difference in the yield of Poria cocos depending on the cultivation method and environment. Therefore, this research was carried out to secure stable yield of Poria cocos using plastic house and bed soil. Methods and Results : The inoculation time was in mid-April 2016 and the inoculation amount was 3/4 lb each in pine trees of 60 (L) × 10 (D) ㎝. The inoculated wood was buried directly in the bed by soil composition and growth characteristics investigated after cultivating in plastic house from April to September, 2016. The composition of the bed soil was 6 treatments using peatmoss, cocopeat and perlite. The combination ratio ranges of peatmoss, cocopeat and perlite were 0 to 80 %, 0 to 60 % and 20 to 60 %, respectively. We assumed that the combination ratio of peatmoss (organic material) 60 % : perlite(inorganic material) 40% is good for the growth of the Poria cocos. In this ratio, instead of peatmoss, the amount of cocopeat was replaced by 20, 40, and 60 %, respectively. The pH and EC range of bed soil was 4.8 - 5.6, 0.7 - 2.2 dS/m, respectively. pH and EC tended to be lower in treatments with only peat moss and perlite. On the other hand pH and EC tended to raise with increasing cocopeat content. Volumetric soil water content and soil temperature were in the range of 13 - 28 % and 20 - 29℃, respectively, during the period of July to September. Soil water content tended to be higher with increasing cocopeat and peatmoss content and soil temperature tended to be lower. The degree of initial hyphal development and sclerotia formation were good in all treatments compared to the control. However, the number of sclerotia was 6 times higher in the ② treatment than in the control. The size of sclerotia was also the best at 12 (L) × 8 (W) ㎝ in the ② treatment. Conclusion : As a result of cultivating Poria cocos in plastic house, growth characteristics were different according to composition of bed soil. The first reason for this result is that the difference of soil moisture content depending on organic matter content affected the soil temperature. Actually, the average soil water content and soil temperature of ① treatment (organic material 80% ) showed 24% and 22℃ respectively during July to September, and no sclerotia was formed. However, in the ② treatment(organic material 60%), the average soil moisture and soil temperature were 14% and 26℃ during July to September, respectively and sclerotia formation was good. Another reason is that the pH of the bed soil affects the formation of sclerotia. Overall, the degree of hyphal development was good in low pH treatments, but no significant difference was found between the pH and the formation of sclerotia.