Russia has very rich biodiversity because it is spread geo-morphologically regions which have very diverse climate character as well as it had carried out to do 1,842 times collecting missions in the world. The plant genetic resources of N.I. Vavilov Institute Gene bank collection have been conserved over 324 thousand germplasm accessions of 155 botanical families, 376 genera, 2,169 species. PGR in VIR have conserved in paper bags in aluminum boxes at room temperature for active working collections and also other PGRs were conserved in Long-term storage at – 100C and short-term storage at+40C at aluminum bags in plastic boxes from 2002. Results of studies of genetic potential of cultivated crops and their wild relatives have been processed into “Cultivated flora” and monographs, including new data of phylogeny, taxonomy, genetics, cytology, biochemistry, molecular biology, physiology, immunity. As a result of primary evaluation every year around 1000 new sources for breeding have been selected. The germplasm in VIR are distributed for 20,000 accessions per year for the new breed upbringing and genetic resources research is about 50%. Therefore, securing of resources is steadily seed with a variety of climates, but to secure the VIR collected from all over the world is worth. In addition, there is a lot of variety of vegetables and minor cereals ensure crops, the genetic resources of vegetables and minor cereals are conserved in VIR will be able to take advantage of a variety of vegetables and minor cereals resource utilization study, if used to conserved the landraces in VIR.

조지아는 지형학적으로 기후대가 아주 다양하게 분포함에 따라 생물다양성이 풍부한 국가이다. 조지아 종자 유전자원은 구 소련시절 러시아 바빌로프생물산업연구소에서 보존되어 오다 독립하면서 육종가들이 육종재료로 활용하기 위하여 개별로 냉장고에 보존하고 있다, 영양체 유전자원은 구 소련시절 수집 및 도입된 자원 중 과수자원은 배262 품종과 사과 147품종을 보존한 이후 1969년 사과 495품종과 배 200품종 등을 수집 보존하였고, 현재 조지아 농업대학에서 포도 유전자원은 지금까지 조지아 지역에 시험장, 농가 등에 산재되어 있던 약 2,664점을 1968년 이후 보존 관리하면서 육종 및 재배 등 연 구를 수행하고 있다. 조지아는 과수유전자원 뿐만 아니라 종자유전자원과 마늘, 감자 등 영양체유전자원도 풍부하다. 조지아는 밀, 보리, 옥수수, 해바라기, 감자 등을 많이 생산하고 있고, 강낭콩 등 두류작물 생산도 높은 편인데, 대부분 재배작물들이 조지아 재래종이다. 따라서 종자자원 등 1년생작물의 유전자원의 확보도 기후대가 다양한 이 나라에서 확보하는 것은 가치 있는 일이다. 또한 다양한 채소류 작물도 많은 량이 재배되고 있는데, 채소류 유전자원이 부족한 우리나라는 이 나라에서 재래종 자원을 확보하여 이용한다면 다양한 채소자원 육종연구에 활용할 수 있을 것이다.

This is presented general consideration for the establishment and management of germ-plasm collection. The topics addressed concern the establishment of the collection, the acquisition andentry of the plants into the collection, germplasm health issues, a presentation of the various conserva-tion methods available to germplasm collections and collection management procedures. Many impor-tant varieties of field, horticultural, root and tuber crops are ether difficult or impossible to conserve asseeds or reproduce vegetatively. Genetic resources of such plant species are generally conserved in fieldgenebanks. field genebanks provide easy and ready access to conserved material for research as well asfor use. For a number of plant species the alternative methods have not been fully developed so that theycan be effectively used. For many species, field genebank is one of the components of complementarystrategy for the conservation of vegetative germplasm. Despite the importance that field genebank playsin conservation of plant genetic resources, the concepts and scientific principles for establishing andmanaging field genebanks are not very well understood by many plant genetic resources workers. Plant-ing a few plants or tree does not constitute a field genebank. There is more science to it that is needed forcontinue maintenance of genetic diversity in the accessions planted in the field as well as to utilize thediversity being thus maintained. It is well recognized that an appropriate collection and conservationstrategy for a particular plant gene pool requires a holistic approach, combining the different ex situ con-servation techniques available in a complementary manner. Ex situ methods are options available for thedifferent genepool elements that are cultivated species, including landraces and modern varieties, wildrelatives, weedy types, etc. Vegetative germplasm has been maintained in field genebanks may be lostbecause of mite infestations or microbial infections. The level of duplication may also depend on theholdings of other genebanks. Accessions available at another genbank may require fewer duplicates thanunique samples

베트남은 지형학적으로나 기후대가 아주 다양하게 분포함에 따라 생물다양성이 풍부한 국가이다. 생물다양성확보와 유전자원의 안전보존을 위해서 1987년에 베트남 농업 연구청 소속 식물유전자원센터를 설립하여 1996년에 확대 개편하여 운영하고 있다. 베트남 유전자원센터의 정식명칭은 Center of Plant Genetic Resources(PGR)이고, 식물 유전자원의 효율적인 관리를 위해서 지역 연구소를 중심으로 관리기관을 지정하여 식물 다양성 확보, 식물 유전자원 보존 활용을 위한 기술개발 을 촉진하고 있다. 베트남의 국가관리 체계내의 관리기관들은 유용한 유전자원의 수집 관리를 위한 임무를 부여 받았다. 즉 식물자원 센터는 관리체계내의 관리기관의 업무를 조정하고21개 유전자원 관리기관이 맡은 작물을 확실히 관리하여 식물유전자원 유지할 수 있게 할 책임이 있다. 베트남에서 식물 유전자원은 약 300종(species) 25,000점(accessions)이 포장, 기내 및 종자 상태로 보존되어 있는데 매년 500점 이상의 식물자원이 수집되고 1,000점 정도가 분양되어 활용되고 있다. 지난 15년 동안 식물 유전자원센터 소속 관리기관에서 식물 유전자원 보존과 실제적인 이용은 향상되었고, 식물유전자원의 현지 보존도 증가하였으며, 농민과 연계한 현지 보존은 재래종 유전자원의 활용을 촉진시킬 뿐만 아니라 선발 육종으로 이용하고 있다. 그러나 저장시설의 열악성, 포장보존 수집 유전자원의 부적절한 평가, 현지보존의 참여자나 방법론의 부족, 정부지원 예산 및 정책의 부족 등은 유전자원 관리에 중요한 약점이다.

This study was carried out to build a database system for amylose and protein contents of rice germplasm based on NIRS (Near-Infrared Reflectance Spectroscopy) analysis data. The average waxy type amylose contents was 8.7% in landrace, variety and weed type, whereas 10.3% in breeding line. In common rice, the average amylose contents was 22.3% for landrace, 22.7% for variety, 23.6% for weed type and 24.2% for breeding line. Waxy type resources comprised of 5% of the total germplasm collections, whereas low, intermediate and high amylose content resources share 5.5%, 20.5% and 69.0% of total germplasm collections, respectively. The average percent of protein contents was 8.2 for landrace, 8.0 for variety, and 7.9 for weed type and breeding line. The average Variability Index Value was 0.62 in waxy rice, 0.80 in common rice, and 0.51 in protein contents. The accession ratio in arbitrary ranges of landrace was 0.45 in amylose contents ranging from 6.4 to 8.7%, and 0.26 in protein ranging from 7.3 to 8.2%. In the variety, it was 0.32 in amylose ranging from 20.1 to 22.7%, and 0.51 in protein ranging from 6.1 to 8.3%. And also, weed type was 0.67 in amylose ranging from 6.6 to 9.7%, and 0.33 in protein ranging from 7.0 to 7.9%, whereas, in breeding line it was 0.47 in amylose ranging from 10.0 to 12.0%, and 0.26 in protein ranging from 7.0 to 7.9%. These results could be helpful to build database programming system for germplasm management.

A statistical analysis for 3651 genetic resources collected from China (1,542), Japan (1,409), Korea (413), and India (287) was conducted using normal distribution, variability index value (VIV), analysis of variation (ANOVA) and Ducan’s multiple range test (DMRT) based on a data obtained from NIRS analysis. In normal distribution, the average protein content was 8.0%, whereas waxy type amylose and common rice amylose were found to be 8.7% and 22.7%, respectively. The protein contents ranged from 5.4 to 10.6% at the level of 95%. The waxy amylose and common rice amylose ranged from 5.9 to 11.5%, and from 16.9 to 28.5% at 95% confidence level, respectively. The VIV was 0.59 for protein, 0.64 for low amylose, and 0.81 for high amylose contents. The average amylose contents were 18.85% in Japanese, 19.99% in Korean, 20.27% in Chinese, and 25.46% in Indian resources, while the average protein contents were found to be 7.23% in Korean, 7.73% in Japanese, 8.01% in Chinese, and 8.17% in Indian resources. The ANOVA of amylose and protein content showed significant differences at the level of 0.01. The F-test for amylose content was 158.34, and for protein content 53.95 compared to critical value 3.78. The DMRT of amylose and protein content showed significant difference (p<0.01) between resources of different countries. Japanese resources had the lowest level of amylose contents, whereas, the lowest level of protein content was found in Korean resources compared to other origins. Indian resources showed the highest level of amylose and protein contents. It is recommended these results should be helpful to future breeding experiments.

This study was conducted to characterize the amylose and protein contents of 4,948 rice landrace germplasm using the NIRS model developed in the previous study. The average amylose content of the germplasm was 20.39% and ranged between 3.97 and 37.13%. The amylose contents in the standard rice were 4.99, 18.63 and 20.55% in Sinseonchal, Chucheong and Goami, respectively. The average protein content was 8.17% and ranged from 5.20 to 17.45%. Protein contents in Sinseonchal, Chucheong and Goami were 6.824, 6.869 and 7.839%, respectively. A total of 62% germplasm were distributed between 20.06% and 27.02% in amylose content. Germplasm of 81.60% represented protein content of 6.78-9.75%. The distinguishable ranges of amylose contents according to origin were 16.58-20.06% in Korea, 20.06-23.25% in Japan, 23.25-27.02% in North Korea, and 27.02-37.13% in China. In the protein content, approximately 30% of Chinese resources ranged from 9.75 to 17.45%, whereas less than 10% were detected in other origin accessions. Fifty resources were selected with low and high amylose ranging from 3.97-6.66% and 30.41-37.13%, respectively. Similarly, fifty resources were selected with low and high protein ranging from 5.20-6.09% and 13.21-17.45%, respectively. Landraces with higher protein could be adapted to practical utilization of food sources.

Little millet (Panicum sumatrense) is well known for its salt and drought stress tolerance and high nutritional value, but very limited knowledge of genetic variation and genomic information is available. In this study, a total of 779 primer pairs were designed from the 22,961 EST sequences of switchgrass (Pancium virgatum), of which 48 EST-SSR markers were developed based on the trials of transferability of these primers in little millet. The EST-SSR amplicons showed reproducible single band polymorphism and produced a total of 160 alleles with an average of 3.3 alleles per locus in 37 accessions of little millet. The average values of expected and observed heterozygosities were 0.266 and 0.123, respectively. The polymorphic information content (PIC) values were observed in range of 0.026 to 0.549 with an average of 0.240. The genetic relatedness among the little millet accessions was evaluated by neighbor-joining dendrogram, which grouped all accessions into two distinct groups. The validation thus demonstrated the utility of the switchgrass EST-SSR markers in assessing genomic relationships in little millet. The findings from this study could be useful for designing strategies for the identification of diverse germplasm for conservation and future molecular breeding programs for little millet.

The objective of this research was to develop Near-Infrared Reflectance Spectroscopy (NIRS) model for amylose and protein contents analysis of large accessions of rice germplasm. A total of 511 accessions of rice germplasm were obtained from National Agrobiodiversity Center to make calibration equation. The accessions were measured by NIRS for both brown and milled brown rice which was additionally assayed by iodine and Kjeldahl method for amylose and crude protein contents. The range of amylose and protein content in milled brown rice were 6.15-32.25% and 4.72-14.81%, respectively. The correlation coefficient (R 2 ), standard error of calibration (SEC) and slope of brown rice were 0.906, 1.741, 0.995 in amylose and 0.941, 0.276, 1.011 in protein, respectively, whereas R 2 , SEC and slope of milled brown rice values were 0.956, 1.159, 1.001 in amylose and 0.982, 0.164, 1.003 in protein, respectively. Validation results of this NIRS equation showed a high coefficient determination in prediction for amylose (0.962) and protein (0.986), and also low standard error in prediction (SEP) for amylose (2.349) and protein (0.415). These results suggest that NIRS equation model should be practically applied for determination of amylose and crude protein contents in large accessions of rice germplasm.

Understanding the genetic variation among landrace collections is important for crop improvement and utilization of valuable genetic resources. The present study was carried out to analyse the genetic diversity and associated population structure of 621 foxtail millet accessions of Korean landraces using 22 EST-SSR markers. A total of 121 alleles were detected from all accessions with an average of 5.5 alleles per microsatellite locus. The average values of gene diversity, polymorphism information content, and expected heterozygosity were 0.518, 0.594, and 0.034, respectively. Following the unweighted neighbor-joining method with arithmetic mean based clustering using binary data of polymorphic markers, the genotypes were grouped into 3 clusters, and population structure analysis also separated into 3 populations. Principal coordinate analysis (PCoA) explained a variation of 13.88% and 10.99% by first and second coordinates, respectively. However, in PCoA analysis, clear population-level clusters could not be found. This pattern of distribution might be the result of gene flow via germplasm exchanges in nearby regions. The results indicate that these Korean landraces of foxtail millet exhibit a moderate level of diversity. This study demonstrated that molecular marker strategies could contribute to a better understanding of the genetic structure in foxtail millet germplasm, and provides potentially useful information for developing conservation and breeding strategies.

The amount of genetic variability of a species is essential for its survival and adaptation in different environments, and studies of genetic diversity using molecular markers are necessary to understand the genetic structure of a population and to orientate effective strategies of germplasm conservation. The aim of current study was to determine the SSR markers that can be used rapidly and reliably to evaluated the pepper of Bulgaria landraces, and applied the markers to assement of introduce genetic diversity of the pepper germplasm. We used 22 polymorphic microsatellite markers to analysis of genetic diversity within 61 pepper collection of Bulgaria landraces germplasm, all SSR primers pairs produced 80 polymorphic and reproducible amplification fragments. An average value of polymorphic information contents (PIC) were 0.334 with a range of 0.061 to 0.63. The mean values of observed (HO) and expected heterozygosity (HE) were 0.383 and 0.154, respectively, indicating a considerable amount of polymorphism within this collection. A genetic distance-based phylogeny grouped into three distinct groups, which was the landrace, moderate and wilde type, genetic distance (GD) value was 0.540. An average day of flowering time was 53 days with a range of 45 to 60 days. The everage od fruit length and width were 9.38cm with a range 2.1 to 23.6cm, and 3.51cm with a range 0.6 to 8.9cm, respectively. Molecular data were complemented with morphological measurements according to the descriptor list for the pepper collection of Bulgaria landraces germplasm.

Fusarium head blight (FHB), also known as scab, caused mainly by Fusarium graminearum is a devastating disease of wheat in regions that are warm and humid during flowering. In addition to significant yield and quality losses, the mycotoxin deoxynivalenol produced by the pathogen in infected wheat kernels is a serious problem for food and feed safety. Twenty- three Korean cultivars and "Sumai 3", which is a FHB-resistant Chinese cultivar were tested for Type I, Type II resistances of FHB. Three cultivars were identified as resistant in Type I assessment, and two cultivars were resistant in Type II assessment. Genetic variation and relationship among the cultivars were evaluated on the basis of 11 Simple Sequence Repeat (SSR) and 29 Sequence Tagged Site (STS) markers that were linked to FHB resistance Quantitative Trait Loci (QTL) on chromosome 3BS. One SSR and 7 STS markers detected polymorphisms. Especially, using a STS marker (XSTS3B-57), 32.4% of the variation for Type II FHB resistance could be explained. Genetic relationship among Korean wheat cultivars was generally consistent with their released year. These markers on chromosome 3BS have the potential for accelerating the development of Korean wheat cultivars with improved Fusarium head blight resistance through the use of marker-assisted selection.