Palytoxin (PLTX)는 자연계 해양생물독소 중 가장 강력 한 독소로, 전 세계적으로 관리되지 않는 해양생물독소이 다. PLTX는 Oetreopsis, Palythoa, Trichodesmium sp. 등에 서 발견된다. 이러한 해양생물독소가 식용 해산물에서 발 견될 경우, 공중보건에 심각한 위험을 초래할 수 있다. 기 후변화로 인한 Ostreopsis sp.의 서식지 확장은 국내 수산 식품의 PLTX 오염에 대한 우려를 불러일으킬 수 있다. 따 라서, 본 연구에서는 국내에 유통되는 국내산 패류에서 PLTX의 오염도 수준을 분석하였다. 그 결과, 실제 시료에 서는 PLTX의 유의미한 농도가 검출되지 않았다. 이러한 결과는 국내에서 생산되는 패류는 PLTX에 오염되지 않았 음을 기기분석법으로 확인하였으며, 이는 패류 섭취에 대 한 안전성을 제공한다.

이 연구는 신경독성을 나타내는 cyclic imine (CI) 독소 의 한국 내 수입되는 패류 내의 오염 수준을 분석한 것으 로, LC/MS/MS를 통해서 spirolide, pinnatoxin, gymnodimine 유도체 7종을 대상으로 분석하였다. 총 21개의 수입산 패 류를 수집하여 분석한 결과, 뉴질랜드산 홍합에서 SPX C 가 227.84 ppt, 칠레산 홍합에서 PnTX G가 80.42 ppt, 뉴 질랜드산 홍합에서 Gym A가 118.79 ppt로 검출되었다. 이 러한 결과는 수입산 패류에서 CI 독소가 초래할 잠재적인 위협의 가능성을 나타내며, 소비자 건강 보호를 위하여 지 속적인 모니터링의 필요성을 시사한다. 또한 이러한 독소 가 포함된 패류 제품에 대한 규제 조치의 시행이 필요함 을 시사하며, 이를 보다 잘 이해하고 관리하기 위한 지속 적인 연구가 필요하다.

This study is investigated the growth characteristics(number of available stipe, pileus diameter, pileus thickness, stipe length, stipe thickness, object weight, comparison yield ability of 1 cycle) and storage characteristics of ‘Sanjo 701’ (S7) cultivars according to relative humidity. The S7 growth characteristics were investigated by quantifying the growth and the characteristics according to the relative humidity, The storage stability was investigated every 5 days and freshness was measured by ‘Minamide Method’. S7 pileus diameter is The higher the relative humidity was confirmed becomes larger and the more increased relative humidity also increase the comparison yield ability of 1 cycle. However, pileus diameter or stipe length This could not see the big difference in the three treatment groups, the plieus thickness was no significant difference in the treatment of 80% and 95%. The fresh weight of S7 decreased significantly at 80% and 95% relative humidity after 10 days of storage, but decreased continuity in 65% humiditiry. The elongation percentage of S7 pileus was observe in 95% relative humidity, values of L, a and b (SCI), showed the highest L value in 65% and the a value in 95%. b values were similar in 65%, 80% and 95% treatments. On the ‘Minamide Method’ measure freshness was changed from the 10th day of storage at relative humidity of 95%, but humidity of 65% and 80% treatments, it changed after 15 days of storage. In this study growth characteristics and yield were increased at higher relative humidity, but storage stability was decreased. Therefore, it is deemed necessary to change the relative humidity to produce high quality mushrooms.

Genetic linkage maps serve the plant geneticist in a number of ways, from marker assisted selection in plant improvement to map-based cloning in molecular genetic research. Genetic map based upon DNA polymorphism is a powerful tool for the study of qualitative and quantitative traits in crops. The objective of this study was to develop genetic linkage map of soybean using the population derived from the cross of Korean soybean cultivar 'Kwangkyo, and wild accession 'IT182305'. Total 1,000 Operon random primers for RAPD marker, 49 combinations of primer for AFLP marker, and 100 Satt primers for SSR marker were used to screen parental polymorphism. Total 341 markers (242 RAPD, 83 AFLP, and 16 SSR markers) was segregated in 85 ~textrmF2 population. Forty two markers that shown significantly distorted segregation ratio (1:2:1 for codominant or 3:1 for domimant marker) were not used in mapping procedure. A linkage map was constructed by applying the computer program MAPMAKER/EXP 3.0 to the 299 marker data with LOD 4.0 and maximum distance 50 cM. 176 markers were found to be genetically linked and formed 25 linkage groups. Linkage map spanned 2,292.7 cM across all 25 linkage groups. The average linkage distance between pair of markers among all linkage groups was 13.0 cM. The number of markers per linkage group ranged from 2 to 55. The longest linkage group 3 spanned 967.4 cM with 55 makers. This map requires further saturation with more markers and agronomically important traits will be joined over it.