본 연구는 간호대학생의 그릿과 사회적 지지가 대학생활적응에 미치는 영향요인을 파악하기 위한 조사연구이다. 연구 대상자는 간호대학생 196명을 대상으로 하였으며 자료수집은 2020년 7월 1일부터 8월 13일까지 수행하였다. 자료 분석은 SPSS 22.0을 이용하여 기술통계, 상관관계 분석 및 다중회귀분석을 사용하였다. 연구결과, 간호대학생의 대학생활적응은 그릿(r=.328, p<.001)과 사회적 지지(r=.334, p<.001) 와 유의한 정적 상관관계를 보였다. 학년, 그릿 및 사회적 지지는 간호대학생의 대학생활적응에 유의한 영향을 미치는 요인으로 나타났다. 이에 본 연구결과를 바탕으로 간호대학생의 대학 생활 적응을 위해서 그릿과 사회적 지지와 같은 긍정적 요인을 포함시킨 대학생활적응 프로그램 개발 및 적용을 제안한다.

서울시내에 위치한 프랜차이즈 매장과 대형마트 내 판매되고 있는 4종(후라이드, 양념, 간장, 치즈가루 치킨)의 치킨제품 123건에 대하여 당과 나트륨 검사를 실시하였다. 전체 치킨제품의 평균 당 함량은 100 g 당 4.5 g이었으며, 양념치킨(8.7 g), 치즈가루 치킨(4.3 g), 간장치킨(3.6 g), 후라이드 치킨(0.6 g) 순으로 나타났다. 평균 나트륨 함량은 전체 치킨제품의 경우 100 g 당 552.4 mg이었으며, 치즈가루 치킨(627.0 mg), 간장치킨(536.3 mg), 양념치킨(511.2 mg), 후라이드 치킨(448.0 mg) 순으로 나타났다. 프랜차이즈와 대형마트 내 판매 치킨제품을 비교하였을 때, 평균 당 함량의 경우 100 g당 대형마트 내 판매 후라이드 치킨(0.8 g) 과 양념치킨(8.9 g)에서 모두 프랜차이즈의 후라이드 치킨 (0.5 g)과 양념치킨(8.6 g)보다 높았으며, 프랜차이즈와 대형 마트 내 판매 제품에 따른 유의적으로 차이를 보였다(P<0.05). 평균 나트륨 함량의 경우 100 g당 프랜차이즈 양념치킨 (516.0 mg)과 대형마트의 후라이드 치킨(471.5 mg)이 높은 값을 보였으며, 유의적인 차이는 보이지 않았다. 포장량 전체를 섭취했을 경우, 평균 당 함량의 섭취비율은 후라이드 치킨은 모두 WHO에서 권고하는 1일 당류 섭취권고량인 50 g 이하였고, 간장치킨(47.6%)과 치즈가루 치킨(55.4%)은 절반 수준이었으나, 양념치킨은 프랜차이즈(140.4%)와 대형마트(148.8%) 제품 모두 권고량을 넘어섰다. 나트륨은 WHO의 1일 나트륨 섭취권고량인 2,000 mg 대비 섭취비율이 후라이드 치킨은 프랜차이즈(140.1%)와 대형마트(170.3%) 제품과 간장치킨(167.9%)에서 높게 나타났다. 특히, 양념치 킨은 프랜차이즈(203.7%)와 대형마트(208.4%) 제품 모두와 치즈가루 치킨(202.4%)에서 섭취권고량의 2배를 넘어선 것으로 나타났다.

A microfluidic reactor with computer-controlled programmable isocratic pumps and online detectors is employed as a combinatorial synthesis system to synthesize and analyze materials for fabricating CdSe quantum dots for various applications. Four reaction condition parameters, namely, the reaction temperature, reaction time, Cd/Se compositional ratio, and precursor concentration, are combined in synthesis condition sets, and the size of the synthesized CdSe quantum dots is determined for each condition. The average time corresponding to each reaction condition for obtaining the ultraviolet–visible absorbance and photoluminescence spectra is approximately 10 min. Using the data from the combinatorial synthesis system, the effects of the reaction conditions on the synthesized CdSe quantum dots are determined. Further, the data is used to determine the relationships between the reaction conditions and the CdSe particle size. This method should aid in determining and selecting the optimal conditions for synthesizing nanoparticles for diverse applications.

내시경 역행 췌담관조영술 중 경췌중격절개술은 선택적 담관 삽관이 어려운 환자에서 성공률을 높일 수 있는 방법으로 알려져 있다. 최근 후향적 연구에서는 경췌중격절개술이 침형절개도를 이용한 조임근절개술보다 효과적이며 안전한 것으로 보고되었다. 그러나 이번 증례에서 담석췌장염 환자에서 경췌중격절개술 후 벽내 십이지장 혈종 및 십이지장 폐쇄가 발생하였고 이로 인해 저혈량쇼크 등 치명적인 합병증이 발생하였다. 따라서 급성 췌장염이 동반된 환자에서 내시 경역행췌담관조영술 중 경췌 중격절개술을 시행한 후에는 벽내 십이지장 혈종과 같은 합병증 발생 여부를 주의 깊게 관찰할 필요가 있다고 생각된다.

In everyday network changing field, permanent service is being an issue. Network disconnection phenomenon is directed to financial damage to service providers. Some companies guarantee on network maintenance, but there is a permitted limit according to their company regulations. If customers overuse the limitation, extra pay will be placed. Moreover, network disconnection affects critical in some fields with using network. Permanent service becomes indispensable in stock, finance, online game, and other fields. Additionally, it should be possible to pre-detect and block abnormal traffic area, improve network infrastructure secureness and optimization, and reduce damage recovery cost. MCS(Multi Connection System) can provide permanent service even if a connection is cut because it provides multi-connection. It can also isolate the attacker when it detects DDoS attack, so it provides smooth service. Also, the benefit from this structure is that the real server can be hidden so the service can be provided continuously. It prevents damages from network cutting, reduces management cost, maintains the best performance, and increases convenient on management and maintenance. Stable and continuous service can be provided with managing network through MCS.

Panax ginseng C.A. meyer (family: Araliaceae) is a perennial crop that has been widely used as a traditional medicine in Korea. Various P. ginseng cultivars exhibit a range of morphological and physiological traits as well as genetic diversity. To elucidate the differences of primary metabolism underlying such genetic diverstiy, we performed primary metabolite profiles in adventitious roots from five Panax ginseng cultivars using gas chromatography-mass spectrometry (GC-MS). The GC-MS analysis revealed eight primary metabolites as biomarkers and allowed us to classify the five cultivars into three groups. We selected three cultivars to represent each group and analyzed their transcriptomes by Illumina sequencing. We inspected 100 unigenes involved in seven primary metabolite biosynthesis pathways and found that 21 unigenes encoding 15 enzymes were differentially expressed among the three cultivars. Integrated analysis of transcriptomes and metabolomes revealed that the ginseng cultivars differ in primary metabolites as well as in the putative genes involved in the complex process of primary metabolic pathways. Our data derived from this integrated analysis provide insights into the underlying complexity of genes and metabolites that co-regulate flux through these pathways in ginseng.

The generation and analysis of genomic resources information are essential to understand genomic features of crops. Even though medicinal component and its effect of Panax ginseng was well studied, the genomic study has been recently started. The ginseng genome has been known to undergo two rounds of whole genome duplication (WGD), therefore we investigated an evidence of WGD in ginseng draft sequence for understanding current ginseng genome structure. Four paralogous gene-rich genome blocks were found, consisted by eight scaffolds, using about 3.0 Gb whole genome draft sequence and 48,821 unigenes of P. ginseng generated by whole genome shotgun sequencing. The eight scaffold sequences were ordered and connected into four genomic blocks, using zig-zag extension within scaffold sequences recently duplicated. The paralogous scaffold pairs that were recently duplicated showed high sequence conservation in genic and non-genic regions. However, paleo duplicated paralogue scaffold sequences showed little conservation only in genic regions. Finally, a total of 110 paralogous gene pairs and its expression were identified from recently and paleo duplicated scaffold pairs, which were co-linear among four genomic blocks. This study provides the first insight into duplicated genome structure of ginseng and will be a valuable information for further ginseng genomics including improvement of draft sequence quality, chromosome anchoring of scaffolds, and genetic mapping.

Genome duplication is an abundance phenomenon and in plant kingdom and consequently formed paralogous region. Korean ginseng (Panax ginseng C.A. Meyer) has a possibility of tetraploid by comparing chromosome numbers of relative species. During development of EST-SSR markers in Korean ginseng, most of primer sets have produced multiple bands in gel electrophoresis. In this study, for identifying origin of multiple bands, five EST-SSR markers showing multi-band were selected and two bands around expected size were sequenced. Sequence comparison classified the multiple bands into individual loci. Two bands can be identified by SNP or InDel variation with number of SSR units. Sequencing result represented that paralogous loci with high similarity were existence caused by recent duplication. One clear band were amplified with newly designed locus specific primer picked from SNP variation. SNP and InDel polymorphism between paralgous loci were useful for identifying each locus. This study will provide better understanding of ginseng genome and will be helpful for development of DNA markers.

Ginseng (Panax ginseng C.A. Meyer) is the most famous medicinal herb in East Asia. Although medicinal components and their functions have been widely investigated, ginseng has been regarded as an underdeveloped crop in genetics and genomics research areas. This study was conducted to elucidate the structure and evolution of the ginseng genome by analyses of expressed sequence tags (ESTs) and bacterial artificial chromosome (BAC) sequences. The EST analysis based on the calculation of synonymous substitutions per synonymous site (Ks) in paralog and ortholog pairs revealed that two rounds of polyploidy events occurred in the common ancestor of ginseng and American ginseng (P. quinquefolius L.) and subsequent divergence of the two species. The sequence analysis of repeat-rich BAC clones characterized the major component of the ginseng genome, long terminal repeat retrotransposons (LTR-RTs). The LTR-RTs were classified into five main families, of which three (PgDel, PgTat, and PgAthila) belonged to Ty3/gypsy and the other two (PgTork and PgOryco) to Ty1/Copia. High abundance of the LTR-RTs were revealed by whole genome shotgun (WGS) read mapping and fluorescence in situ hybridization (FISH) analysis. Particularly, the most abundant PgDel family have played major roles in expanding heterochromatic regions as well as remodeling euchromatic regions. Biased intensity of the PgDel2 FISH signals on half the total chromosomes demonstrates the allopolyploid feature of ginseng genome. Insertion time estimation of each LTR-RT implied that LTR-RTs have proliferated after the recent polyploidization of ginseng. These results suggest that the ginseng genome of the present day has been expanded and evolved by two rounds of polyploidization and accumulation of LTR-RTs.

Next generation sequencing (NGS) approaches can also be useful tool for characterization of organelle genomes. We generated chloroplast (CP) genome sequences of two Korean ginseng cultivars, Chunpoong and Yunpoong, based on reference-guided assembly using whole genome NGS data. We used 0.5x of P. ginseng genome NGS reads to assemble CP genome. Of the NGS reads used, about 6% were mapped to the reference CP genome with mean coverage of 94x due to high copy number of CP genome in plant cell. CP genomes of the two cultivars were predicted to be 156,248 bp and 156,355 bp in length and showed about 0.1% differences at nucleotide level, compared to reference CP genome sequenced from P. ginseng (Acc.no. NC_006290), whereas difference between CP genomes of the two cultivars is very rare. In this study, we developed the molecular marker to perform taxon identification and also to elucidate phylogenetic relationship among Korean ginseng cultivars. Now, we are analyzing the CP genomes of other P. ginseng cultivars together with other Panax species including American ginseng and Panax related species.

Genetic map provides basic and important informations for breeding. Therefore, genetic map construction is a essential process in plant research. Panax ginseng is one of the most famous medical plant in the world. However, genetic informations of this medical plant for breeding are not enough. Because of little informations, genetic map construction of panax ginseng provides very useful information for breeding. Using Solexa next generation sequencing (NGS) technology, we have been produced a lot of expressed sequence tags (ESTs) and whole genome sequences from Chunpoong (368 Gb) and Yunpoong (6 Gb) cultivar. To develop large amount of DNA markers and thus construct high resolution genetic map, we inspect large scale of SSR motif and putative SNP sites which can be used as dCAPs markers using produced ginseng’s sequence data. As a result, we can find a number of DNA markers that have polymorphism between Yunpoong and Chunpoong cultivar. These developed DNA markers were analyzed for F2 population of Yunpoong x Chunpoong to find markers showing Mendelian segregation ratio 1:2:1.

Groundwater recharge characteristics in a fractured granite area, Mt. Geumjeong, Korea. was interpreted using bedrock groundwater and wet-land water data. Time series analysis using autocorreclation, cross-correlation and spectral density was conducted for characterizing water level variation and recharge rate in low water and high water seasons. Autocorrelation analysis using water levels resulted in short delay time with weak linearity and memory. Cross-correlation function from cross-correlation analysis was lower in the low water season than the high water season for the bedrock groundwater. The result of water level decline analysis identified groundwater recharge rate of about 11% in the study area.