South Korea has over 0.38 million of managed honey bee (Apis cerana) colonies before 2009 years ago, which produce the highest quantity of honey in the Korea; however, almost colony (90%) were collapsed by Korean Sacbrood Virus (KSBV) in South Korea. Korean Sacbrood Virus (KSBV) is the pathogen of A. cerana Sacbrood disease, which poses a serious threat to honeybee A. cerana, and tends to cause bee colony and even the whole apiary collapse. Colony collapse of A. cerana was first reported on the Pyeong-Chang of the South Korea in 2009. Several scientists and governments has been tried research for cure the sacbrood disease in A. cerana colony by medicines and management techniques. Unfortunately, The sacbrood disease dosen’t improve. So, we were developed a better breed of A. cerana for resistance of sacbrood virus by selection and then artificial insemination. A. cerana breeding technique was first successful applied with A. cerana in Korean. Queens was grafted from sacbrood resistance line and then it was growing in sacbrood disease colony that was survived 100%. Altogether selected 18 queens were artificially inseminated and 2,000 drones of A. cerana in Korea was used to evaluate amount of semen collection. We are select two scabrood resistance A. cerana line (R and H). R line be used for rearing the Queen. Drone was reared in H line colony. The RH hybrid were not infected sacbrood virus even spread sacbrood virus (2×106). RH colonies have very excellent hygienic behavior, brood, and sacbrood disease resistance activity.

The black pine bast scale are the main insects of the Japanese black pine forest in Korea, and the distribution is spreading around the Japanese black pine forest in the east and west coast. However, after the nationwide survey in 2010, there has been no investigation on the distribution and spread of these, so we conducted the 2017 and 2018 surveys to determine the extent of the northern area inland or east and west. In the 2017 survey, outbreaks were observed in 35 areas from 47 dong or myeon of 14 cities or counties, mainly in areas not previously located in the outbreak area. In the 2018 survey, additional outbreaks were confirmed at 14 out of 23 areas in the East Coast region, and damage was detected at 27 out of 28 points in the inland area of the South Sea and distribution was found at 16 out of 17 in the west coast.

후각 신경 시스템은 일반 생활 환경에서 많은 다양한 화학 물질을 인식하고 구별한다. 곤충에서는 다양한 화학 물질을 기호적 또는 회피적인 특이성을 부여하고 이를 구분해 낼 수 있는 고도로 발달된 후각신경 수용체들로 구성된 냄새 맡는 (odorant-gated) 이온 채널 군을 진화시켰다. 최근에 후각 수용체와 단백질을 포함한 olfaction 관련한 진딧물 게놈 밝혀졌고, 초파리에서 다양하게 분화되어 있는 후각 수용체들이 보고되고 있다. 후각 신경 수용체의 유전체는 매우 높은 보전적인 염기 서열을 가지고 있으며, 체계적인 신호 전달 시스템을 갖추고 있다. 대표적 수용체인 odorant-gated ion channels comprised of a highly conserved co-receptor (Orco)는 중심 구멍 주위에 대칭 적으로 배열된 4 개의 서브 유닛을 갖는 homotetramer 채널 구조를 가지고 있다. 이는 인체 내에 존재하는 7-transmembrane receptor와 매우 유사한 구조를 형성하고 있고, 신경전달물질의 수용체와 매우 유사한 구조적 형태 및 gating mechanism을 가지고 있다. 본 연구에서는 초파리에서 분리한 후각 신경 수용체 하위 유형인 OR65 유전자 분리하여 세포 발현 시켜 Xenopus oocyte를 이용하여 Whole cell voltage clamp recording을 실시하였다. 본 수용체의 성공적인 발현 이후 유해 해충 유인제 개발 회사인 마이크로자임의 미생물 배양 추출물을 이용해서 후각 신경 수용체 활성 조절 여부를 연구하였다. 미생물 배양 추출물을 10,000배 희석한 recording media에서 수용체의 활성을 확인하였고, 이를 농도 별로 처리하여 농도 의존성 수용체 활성 작용을 확인하였다. 따라서 곤충의 후각 신경 수용체 활성 조절 시스템을 이용하여 유인물질 또는 기피 물질을 발굴할 수 있으며, 본 연구를 통해서 MZ01은 곤충 수용체 OR65를 활성 시킴으로써 유인 현상을 나타내며, 본 연구를 통해서 현장에서 검증된 미생물 배양 추출물의 성능을 과학적 분석으로 결과를 제시하였다.

ICT 기반의 해충 이미지 전송 시스템은 담배거세미나방과 파밤나방을 예찰하고 방제하는데 필요한 효과적인 유인 해충 전송수단이다. 시설 온실내 담배거세미나방과 파밤나방을 유인하기 위하여 스마트 트랩을 이용하였다. 스마트트랩은 나방류 해충유인을 위해 성페로몬 4종 장착하였으며 유인된 해충은 특수 카메라를 이용하여 이미지 촬영이 가능하였다. 또한, 해충유인 발광트랩 처리구는 황색광을 사용한 발광 트랩을 사용하였다. 또한 대조구로 기존에 널리 사용되는 델타트랩은 한 종류의 성페로몬을 장착하여 유인력 검정에 이용하였다. 성페로몬 한 종류만 사용한 델타트랩 처리는 담배거세미나방 페로몬 루어를 사용하였고, 4종류의 성페로몬을 설치한 스마트 트랩 처리구는 담배거세미나방, 뒷날개흰나방, 파밤나방, 왕담배나방용 성페로몬 루어를 사용하였다. 실험은 2018년 6월 1일부터 8월 31일까지 함안 시설원예연구소의 13m2의 소형비닐하우스에서 수행하였으며 공시작물은 파프리카 ‘시로코’ 품종과 토마토 ‘데프니스’ 품종을 사용하였다. 나방류 해충의 유인력 검정결과 파프리카와 토마토를 식재한 온실 모두 성페로몬 루어 한 종류를 설치한 델타트랩보다 유인발광 트랩 처리구에서 높은 나방류 유인율을 나타내었다. 시험기간 유인발광 트랩의 경우 유인 포획된 총 나방수는 파프리카 온실에서 평균 97마리, 토마토 온실에서 75마리가 유인 포획되어 가장 높았다. 그러나, 성페로몬 1종 및 4종을 설치한 델타트랩 및 스마트트랩처리구는 두 개의 온실 모두 평균 5마리 이하의 유인율을 나타내었다. 따라서, 시설내 나방류 예찰 및 유인 방제하기 위해서는 기존 성페로몬만 사용하기 보다는 유인발광 트랩을 함께 설치하는 것이 나방류 유인포획에 효과적이라고 판단되었다.

The hypopharyngeal gland (HPG) of the honeybee worker produces royal jelly (RJ) and has a developmental cycle closely related to the division of labor. In this study, we investigated to compare the HPG acini diameter of differently aged worker bees with high royal jelly producing colony (HRC) or less producing colony (LRC). Additionally, we also evaluated whether the fresh weight of the head is a reliable indicator of the developmental status of HPG. The HRC showed a significantly higher RJ production about two-times as compared with those of the LRC. We measured the HG-diameters on days 1, 3, 6, 9, 12, 15. The microscopic analysis revealed that the acini size of the HRC was significantly larger than the LRC. In addition, the acini diameter of HRC was 15% longer than the LRC on the first day after emerging. It was shown that the fastest development during 3 days which is preparing for nurse the brood. The HPG acini diameters increased in both colonies in a similar fashion until day 12 and then decreased. We also compared the fresh head weight of the experimental colonies, differences were similar to the development of HPG. Therefore, high royal jelly production may have a positive correlation between HPG acini size and the fresh head weight.

Salvia miltiorrhiza (Danshen) is perennial plant and commonly used in traditional Chinese medicine to treat numerous diseases like cardiovascular and cerebrovascular diseases, coronary heart disease, myocardial infarction, stroke and some viral diseases. Nevertheless, no study has been conducted on the antiviral properties of crude extract of Salvia miltiorrhiza against Influenza virus. In an attempt to identify new potential anti-influenza virus agents, 200 natural oriental herbal medicines were screened and we found that Salvia miltiorrhiza has a potential anti-influenza effect. Therefore, in this study, we investigated the protective effect of aqueous extract from Salvia miltiorrhiza against divergent influenza A subtypes using murine model of influenza A infection. Effective dose of aqueous extracts of Salvia miltiorrhiza in BALB/c mice displayed higher survival rate and lower lung viral titers when challenged with lethal doses of influenza A subtypes ({A/Aquatic bird/Korea/ W81/2005(H5N2)}, {A/PR/8/34(H1N1)}, {A/Aquatic bird/Korea/W44/2005(H7N3)} and {A/Chicken/ Korea/116/2004(H9N2)}). In vivo results exhibited that Salvia miltiorrhiza induced prophylactic effect in BALB/c mice against Influenza virus by disrupting viral replication or preventing viral infection by creating an antiviral state in the lungs. Taken together, the use of aqueous extracts of Salvia miltiorrhiza as an orally active antiviral agent, will be potential candidates for prophylactic treatments against Influenza A virus for humans and animals.

Near infrared spectroscopy (NIRS) is a rapid and accurate method for analyzing the quality of cereals, and dried animal forage. However, one limitation of this method is its inability to measure fermentation parameters in dried and ground samples because they are volatile, and therefore, respectively lost during the drying process. In order to overcome this limitation, in this study, fresh coarse haylage was used to test the potential of NIRS to accurately determine chemical composition and fermentation parameters. Fresh coarse Italian ryegrass haylage samples were scanned at 1 nm intervals over a wavelength range of 680 to 2500 nm, and optical data were recorded as log 1/reflectance. Spectral data, together with first- and second-order derivatives, were analyzed using partial least squares (PLS) multivariate regressions; scatter correction procedures (standard normal variate and detrend) were used in order to reduce the effect of extraneous noise. Optimum calibrations were selected based on their low standard error of cross validation (SECV) values. Further, ratio of performance deviation, obtained by dividing the standard deviation of reference values by SECV values, was used to evaluate the reliability of predictive models. Our results showed that the NIRS method can predict chemical constituents accurately (correlation coefficient of cross validation, R2 cv, ranged from 0.76 to 0.97); the exception to this result was crude ash (R2 cv = 0.49 and RPD = 2.09). Comparison of mathematical treatments for raw spectra showed that second-order derivatives yielded better predictions than first-order derivatives. The best mathematical treatment for DM, ADF, and NDF, respectively was 2, 16, 16, whereas the best mathematical treatment for CP and crude ash, respectively was 2, 8, 8. The calibration models for fermentation parameters had low predictive accuracy for acetic, propionic, and butyric acids (RPD < 2.5). However, pH, and lactic and total acids were predicted with considerable accuracy (R2 cv 0.73 to 0.78; RPD values exceeded 2.5), and the best mathematical treatment for them was 1, 8, 8. Our findings show that, when fresh haylage is used, NIRS-based calibrations are reliable for the prediction of haylage characteristics, and therefore useful for the assessment of the forage quality.

Oyster mushrooms are popular and physiologically beneficial to human health. This study was conducted to investigate the effect of dietary supplementation of oyster mushrooms such as Pleurotus ostreatus, P. pulmonarius, and P. citrinopileatus on high cholesterol fed rats. Five-week old female Sprague-Dawley albino rats were divided into three groups of 8 rats each. Feeding a diet containing a 5% fruiting body powder of Pleurotus species to high cholesterol fed rats reduced plasma total cholesterol (TG), triglyceride (TG), low-density lipoprotein (LDL). Feeding each oyster mushroom also significantly reduced body weight in high cholesterol fed rats. However, it had no adverse effects on plasma albumin, creatinine, blood urea nitrogen, uric acid, glucose, total protein or enzyme profiles such as glutamate oxalate transaminase (GOT), glutamate pyruvate transaminase (GPT), and alkaline phosphatase (ALP) which are related to kidney function. Feeding each mushroom increased total lipid and cholesterol excretion in feces. A histological study of hepatic cells by conventional red O staining showed normal findings in mushroom-fed hyperlipidemic rats. These results suggested that diet supplementation of 5% fruiting body power of Pleurotus species provided health benefits by acting on the atherogenic lipid profile in high cholesterol fed rats.

Ochratoxin A, which is frequently detected in cereals and infant diets worldwidely, is a mycotoxin to damage mainly the kidney and liver. Because ochratoxin A is highly thermostable compound. it is necessary to study ways of reducing level of ochratoxin A by controling processing steps. However, food processes, including extrusion, expansion, roasting, and steam cooking, which are used in order to mitigate the contents of ochratoxin A, are known to produce polycyclic aromatic hydrocarbons, which are generated from radicals decomposed by pyrolysis. Therefore, this study analyzed the levels of 4 polycyclic aromatic hydrocarbons, benz (a) anthracene, chrysene, benzo (b) fluoranthene and benzo (a) pyrene in rice-based products made in high pressure and heating process. Rice samples were finely ground, and homogenized samples were alkaline treatement with 1 M KOH/EtOH and extracted with liquid-liquid extraction method using n-hexane. The extracted solution was pretreated with a silica cartridge. The purified solution was dried with nitrogen gas and dissolved in 1 mL of dichloromethane and injected into GC/MSD. We had overall recoveries for 4 polycyclic aromatic hydrocarbons spiked into rice samples ranging from 92.8 to 110.2%. The limit of quantitations of benz (a) anthracene, chrysene, benzo (b) fluoranthene and benzo (a) pyrene in rice-based product were 0.19 ng/g, 0.38 ng/g, 0.51 ng/g, and 0.31 ng/g, respectively. However, these 4 polycyclic aromatic hydrocarbons in all processed rice samples were not detected.

In order to perform the biological investigation of coffee extract containing different molecules, it would be necessary to develop in vitro experimental system rather than animal experiment. Although the animal experiment treated via oral intake or intravenous injection may disclose the whole systemic effect, the in vitro cell culture experiment would be more convenient to analyze direct cellular effect of coffee extract than animal experiment. Therefore, this study was aimed to develop a dialysis method for the crude coffee extract to perform the biological investigation using murine macrophage cell line, RAW 264.7. First of all, the RAW 264.7 cells treated with dialyzed coffee extract were observed, and subsequently their protein extracts were analyzed by gel filtration chromatography, thin layer chromatography, and immunoprecipitation high performance liquid chromatography (IP-HPLC). Resultantly, it was found that the low dose (20μg/mL) of dialyzed coffee extract, about 5 cups of ordinary coffee drinking for human adult, enhanced the growth of RAW 264.7 cells by increased expression of β-actin and Ki-67, and also induced the anti-inflammatory effect by decreased expression of NFkB, TNFα, and LC3 contrast to the high dose (40μg/mL) of dialyzed coffee extract. The low dose of dialyzed coffee extract produced almost no harmful effect on RAW cell culture for 12 hours, rather than it produced stimulatory effect on RAW cells by increasing the cell number and enhancing the protein expression of β-actin, Ki-67. Therefore, it was thought that the low dose of dialyzed coffee extract is applicable to cell culture experiment without difficult purification procedures of coffee elements. In addition, as the contrast cellular effect between the low and high dose of coffee extract was found in this study, it was also presumed that the low dose of coffee extract may play an important role in the inflammatory reaction of murine macrophages.