참다슬기 아가미 조직으로부터 heat shock protein 70 유전자를 분리 · 동정하였다. 참다슬기 HSP70 cDNA의 open reading frame (ORF)는 1,917 bp로 639개의 아미노산을 암호화하여 분자 량은 약 70 kDa으로 예측되었다. 생물정보학 배열분석에 의해 HSP 유전자 기능과 관여되어 있는 3가지 주요 signature motifs와 보존된 도메인을 확인하였다. 계통학적 분석을 통하여 참 다슬기 HSP70 유전자는 왕우렁이 Pomacea canaliculate와 같은 클러스트에 포함된다는 사실을 확인하였다. 수온 및 염분 변화에 따라, 참다슬기 HSP70 mRNA 유전자 레벨은 유의적으로 증 가하였으며(p < 0.05), 이는 외부자극요인을 파악할 있는 분자생물학적 마커로서 활용될 수 있 을 것으로 사료된다.

Potential utility of 14 candidate housekeeping genes as normalization reference for RT-qPCR analysis with developmental samples (fertilized eggs to late veliger larvae) in Pacific abalone Haliotis discus hannai was evaluated using four different statistical algorithms (geNorm, NormFinder, BestKeeper and comparative ΔCT method). Different algorithms identified different genes as the best candidates, and geometric mean-based final ranking from the most to the least stable expression was as follow: RPL5, RPL4, RPS18, RPL8, RPL7, UBE2, RPL7A, GAPDH, RPL36, PPIB, EF1A, ACTB and B-TU. The findings were further validated via relative quantification of metallothionein (MT) transcripts using the stable and unstable reference genes, and expression levels of MT were greatly influenced according to the choice of reference genes. In overall, our data suggest that RPL5 and RPS18, either singly or in combination, are appropriate for normalizing gene expression in developmental samples of this abalone species, whereas ACTB, B-TU and EF1A are less stable and not recommended. In addition, our findings propose that standard deviations in geometric ranking as well as geometric mean itself should also be taken into account for the final selection of reference gene(s). This study could be a useful basis to facilitate the generation of accurate and reliable RT-qPCR data with developmental samples in this abalone species.

국립 9.11 뮤지엄의 목적은 기억, 인식, 회복에 있으며, 9.11을 어떻게 기억할 것인가의 문제가 주요 논점이었다. 특히 기억은 9.11에 대한 미국의 기억이 탈역사, 탈기념의 맥락에 있음을 확인시켜 주었다. 초기에 형성된 ‘거리 추모비’나 마이클 아라드의 <부재의 반추>, 시민 참여의 공모전, 공청회, 포럼 등은 모두 기억의 목적을 향하였다. 그러나 테러 1주기 이후, 9.11에 대한 기억은 점차 전통적인 기념 방식으로 회귀하였다. 전시된 생존 오브제들은 예외주의의 수사로 기능하였으며, 자유수호의 의지, 불의에의 저항 등 국가 정체성과 관련된 서사가 내재되었다. 또 한 함께 전시된 빈 라덴의 벽돌은 국가적 보복행위의 상징물로서, 애도의 빚을 청산하려는 자기 모순을 보였다.

Most of the red ginseng (RG) products contain active substances derived from hot water or alcohol extraction. Since active substances of RG are divided into two two types: water-soluble and liposoluble, water or alcohol is needed as an extraction solvent and this leads the different extraction yields and components of the active substances. To overcome the limit, whole red ginseng powder can be used and consumed by consumers. In this study, the physicochemical properties and extractable active substance contents of variable-sized RG powder (158.00 μm, 8.45 μm, and 6.33 μm) were analyzed, and dispersion stability was measured to investigate the suitable size of RG powder for industrial processing. In the results, no significant difference was found from the changes in color intensity and thiobarbutric acid tests at 4°C, 25°C, and 40°C for 4 weeks. There was no significant difference on the production of antioxidants and ginsenoside among the samples (p>0.05). In dispersion stability, RG-158.00 μm was precipitated immediately, and the dispersion stabilities between RG-8.45 μm and RG-6.33 μm showed no significant difference. It implies that fine RG is suitable for the production process. With further study, it seemed that the physicochemical effects of RG particle sizes can be clearly revealed.

The conditions of blanching, freezing, and thawing for Shepherd’s purse (Capsella bursa-pastoris [L.]) were studied to improve its quality attributes during frozen storage. The washed samples were blanched with water at 100oC for 10, 20, 30, and 40 s. They were then soaked in cold water. The quality characteristics of the samples such as pH, texture, hardness, color value, and total viable cell were analyzed. The samples blanched for 30 s were frozen and thawed under several conditions and their physicochemical characteristics were analyzed. The pH value of raw samples was higher than that of the blanched sample. The values of brightness (L*), redness (a*), and yellowness (b*) decreased after blanching except the L* value of root at the condition of blanching for 40 s. Upon considering the bacterial cell counts, the proper condition was determined to be 30 s of blanching time. Blanching for 30 s was the optimal pre-treatment for minimizing the quality deterioration of the shepherd’s purse for freezing. Regarding its color change and microbial safety, faster freezing and thawing methods made less changes on the quality of frozen samples. Consequently, immersion freezing and radio-frequency thawing were the most effective in minimizing the quality change.

There are a lot of types of wild vegetables such as Colocasia esculenta (L.) Schott stem in Korea. However, the consumption of these wild vegetables is restricted because their storage decreased dramatically after harvest. To maintain original quality of vegetables, pre-treatments such as blanching and drying are important. But conditions for these treatments were still not optimized for many vegetables including Colocasia esculenta (L.) Schott stem. Thus, the objective of this study was to set up an optimal pre-treatment method for freezing storage. Colocasia esculenta (L.) Schott stems were peeled and cut equally (10 cm) for sample preparation. Dried samples (D) were dried at 90℃ for 3 h. Blanched samples (B) were blanched in hot water at 100℃ for 2 min. Blanched and dried samples (BD) were blanched and dried as same protocol. Physicochemical properties were analyzed to evaluate the quality including texture, moisture content, total color difference and viable cell count. Raw sample had 6.85 kg/cm 3 of hardness and 78.75 of chewiness whereas B was 6.83 kg/cm 3 of hardness and 7.8 of chewiness. B had the similar value compared to raw samples. Moisture content of raw sample was 94.4% and that of B was 94.1%, though there were not any significant differences between them. ΔE value of B showed lower value than those of the others. Viable cell counts and total coliforms were not detected after treatment, while raw sample had 5.39 log CFU/g of viable cell count without total coliform. Therefore, pre-treatments are essential for microbial safety of samples. All results considered, it is supposed that blanching is the optimal pre-treatment to sustain its original quality of Colocasia esculenta (L.) Schott stems before freezing.