벼밤나방[Sesamia inferens (Walker, 1856)](나비목: 밤나방과)은 벼 줄기를 가해하는 해충이다. 본 연구는 국내 몇 지역에서 벼밤나방의 연 중 세대수를 추정하였다. 북위 38° 부근의 세 지역에서 벼밤나방 성충이 성페로몬트랩에 포획되었다. 이는 국내 모든 지역에서 벼밤나방이 서식하 는 것을 나타냈다. 조사된 지역 대부분에서 단일사인곡선의 유효적산온일도 모델로 추정된 세대별 성충 발생 시기는 관찰된 성충 세대의 발생 시기 와 차이가 없었다. 이 모델을 사용하여 추정된 월동유충은 성충 마지막 세대로부터 유래할 가능성이 있다고 추정되었다. 중남부지역에서 가을동안 벼 포장에서 채집된 유충들을 25°C에서 사육하였을 때, 번데기의 약 70%가 용화전 유충 탈피를 보이지 않았다. 경남 고성에서 3월초에 채집된 유 충은 25°C 사육에서 유충 탈피 없이 용화하여 이 지역에서는 주로 마지막 유충 영기로 월동할 것으로 추정되었다. 모든 결과를 종합하여 벼밤나방 은 북위 38° 부근 지역에서는 주로 2세대, 북위 35.3° ~ 37.3° 사이 지역에서는 3세대가 경과할 것으로 결론지었다. 별도로, 시토크롬 c 산화효소 1 유전자의 염기서열을 분석한 결과, 성페로몬트랩에 포획된 종의 35%가 다른 종이었고, 이들은 형태적으로 벼밤나방과 혼동되지 않았다.

Azuki bean beetle (ABB), Callosobruchus chinensis (L.) is a cosmopolitan field-to-storage pest of legumes. Its female sex pheromone consists of two isomeric components, 2Z-homofarnesal and 2E-homofarnesal. Two day old virgin adults were exposed to sub-lethal fumigation doses (LC25=2.80 and LC50=3.32 mgL-1) of ethyl formate (EF) to see the effects on adult longevity, fecundity, sterility and female sex pheromone production. Longevity (female; P < 0.001, male; P < 0.001), fecundity (P < 0.001) and sterility (P < 0.001) were significantly affected. The Gas Chromatography-Mass Spectrometry for solid phase micro extraction revealed that release of both of the pheromone components were not affected by LC25 dose of EF. However, the fecundity (per female/day) was significantly (P < 0.001) stimulated by the tested EF doses when the female adults were treated irrespective of the male treatment, probably revealing a hormetic effect. This study provides insight into the effect of EF fumigation on biological activities of C. chinensis.

Azuki bean beetle (ABB), Callosobruchus chinensis (L.) is a field-to-storage pest of legumes and its female produces sex pheromone with two isomeric components, 2Z-homofarnesal and 2E-homofarnesal. Two day old virgin adults were exposed to 0, 200, 300, 400, 500 & 600 Gy gamma radiation and effect on longevity, fecundity, sterility and pheromone production were studied. Longevity (female; P < 0.001, male; P < 0.001) and fecundity (P < 0.001) were dose-dependently affected by the gamma irradiation. Both adults were totally sterilized by the tested doses of gamma irradiation as depicted by the null hatchability of laid eggs. Gas chromatography-mass spectrometry for solid phase micro extraction revealed that both of the pheromone components were significantly but not completely reduced by 300 Gy. It is a pre-requisite for a successful sterile insect technology that the sterility of ABB is induced without the total disruption of calling behavior.

Azuki bean beetle (ABB), Callosobruchus chinensis (L.) is a field-to-storage pest of legumes. It is ideal to estimate its field population before it invades the stored legume stuffs. The seasonal occurrence was monitored with rocket traps supplied with synthetic female sex pheromone and with sweep netting during 2015-2016. Harvest damage was evaluated by taking purposive samples of the pods at harvest. Over-wintering stage was identified from the pod samples of left over plants. The results ascertained that October was the peak season of ABB in the azuki bean field. Grain damage and weight loss of the harvested azuki bean were 2.7% and 2.4% respectively, which could be the inoculum for storage loss. The adult emergence only from live pupae collected from pods revealed for its most probable over-wintering stage. In all, this study provides insight into some field ecological parameters of ABB that could be helpful in adopting its management strategies.

Plant essential oils (EOs) exhibit an array of biological activities against insect pests. However, their negative influences on the pheromonal activity of azuki bean beetle (ABB), Callosobruchus chinensis L., the field-to-storage pest of legumes, have not received research attentions. We evaluated the effects of three EOs and their two major components on the attractiveness of male ABBS to synthetic homofarnesal (2E- : 2Z-homofarnesal = 6:4) using Y-tube olfactometry in laboratory and rocket traps in the semi-open vinyl house. The tested EOs of Illicium verum, Croton anisatum and Gaultheria fragrantissima as well as the major components, trans-anethole and methyl salicylate ascertained significant negative effect against homofarnesal both in Y-tube olfactometry and in semi-open vinyl house tracing out a new opportunity of integrating them in ABB management programs both in field and storage.

Ethyl formate (EF) and methyl salicylate (MS) are naturally occurring and generally safe substances for use. Sub-lethaldoses of EF and MS were evaluated to find synergistic fumigation action against azuki bean beetle (ABB), Callosobruchuschinensis (L.). Susceptibility of the ABB to EF was in the order of eggs =adults >larvae >pupae. Adults was most sensitiveto MS, and followed by eggs. Larvae and pupae were highly tolerant to MS. However, in synergistic effects of EF andMS showed that all the stages of ABB were highly sensitive in the order of adults >eggs >larvae >pupae. Highest synergisticratio was found against adults when using LC10 of both EF and MS as compared to other stages. Notably, MS synergizedthe EF exposure to all stages of ABB. This finding paves the way to exploiting synergistic activity of MS and EF againstABB for the safeguard of stored azuki bean grains.

The female azuki bean beetle, Callosobruchus chinensis L., a worldwide notorious pest of azuki beans produces sex pheromone having two isomer compounds; (2Z,6E)-7-ethyl-3,11-dimethyl-2,6,10-dodecatrienals (2Z-homofarnesal) and (2E,6E)-7-ethyl-3,11-dimethyl-2,6,10-dodecatrienals (2E-homofarnesal). We synthesized and evaluated the attractiveness of different blends of the two isomeric compounds using Y-olfactometry in laboratory and rocket traps in the field. The attractiveness pattern of the tested blends in Y-olfactometry was found as 1:9≥6:4>9:1 of 2E-:2Z-homofarnesal. Under field condition also, the 6:4 blend was more attractive than 9:1 blend. The result ascertained the enhanced pheromonal activity of homofarnesal with the increased ratio of Z-component in the blend. This provides an opportunity to synthesize Z-rich homofarnresal with less sophisticated and economically feasible Z-selective method.

잎들깨의 해충인 차응애(Tetranychus kanzawai)와 들깨진딧물(Aphis egomae)에 효과적인 약제를 선발하기 위하여 진주와 밀양의 하우스 잎들깨에서 약제선발 실험을 실시하였다. 차응애에 대해서는 milbemectin 유제, abamectin 유제, cyenopyrafen 액상수화제, chlorfenapyr+cyenopyrafen 액상수화제, hexythiazox 수화제, azocyclotin 수화제의 여섯 약제가 두 지역에서 90% 이상의 방제가를 나타내었고, 들깨진딧물에 대해서는 bifenthrin+clothianidin 액상수화제, spirotetramat 액상수화제, thiacloprid 액상수화제 등 3종의 약제가 두 지역에서 93.7% 이상의 우수한 효과를 나타내었다. 이들약제는 기준량과 배량에서 들깨잎에 어떠한 약해도 나타나지 않았다.

This study was conducted to determine the effect of six different leguminous seeds a) cowpea, b) white kidney bean c) soybean (Cheongja) d) soybean (Daepung) e) mung bean and f) azuki bean on the nymphal development, mortality, adult longevity, fecundity and hatchability of Riptortus pedestris (F.) (Hemiptera: Alydidae). Total developmental time ranged from 18 to 25 days; R. pedestris fed on white kidney bean took the longest time. There was no difference in mortality among the bugs fed on the legumes except those fed on white kidney bean which had the highest mortality (94%). Also, adult bugs fed on white kidney bean showed the shortest longevity. Total number of eggs laid by adult female and the hatchability of the eggs were highest from the bugs fed on cowpea. Higher reproductive success of R. pedestris fed on cowpea suggests that cowpea could be very suitable legume for the bug and it can be used as a trap crop against the bean bug.

Several pentatomids and an alydid are reported inflicting losses in several host plants ranging from leguminous crops to fruit orchards in Korea. Understanding their visual preference would help develop mass trapping or efficient monitoring device. Visual preference of the major pentatomids and an alydid bugs to different colors and light emitting diodes (LED) of different wavelengths were observed. Adults of pentatomid such as Halyomorpha halys Stål, Plautia Stali Scott, and Nezara sp., and an alydid Riptortus pedestris (F.) were exposed to seven different colors (black, yellow, green, blue, white, cream, orange) in a multiple choice tests. Also, adult and second instar nymphs of the bugs were released inside a hexagonal LED boxes consisting of LED lamps of six different wavelengths (375 to 630 nm). Most of the pentatomids showed highest preference to blue color followed by yellow and 530 nm LED. However, R. pedestris showed higher preference to the yellow color followed by blue and black. Second instar nymphs of all the tested species also showed higher preference to 530 nm. Their color and LED lamp preference would help in development of mass trapping device.

근접방사선치료는 일반적으로 외부방사선치료와 병행하여 수행되고 치료단계가 매우 복잡하며 이로 인 해 방사선 사고가 발생될 수 있다. 본 연구에서는 이를 해결하기 위해 근접방사선치료에 사고유형과 영향 분석(Failure mode and effects analysis, FMEA) 방법을 적용하여 프로세스 맵을 구성하고 이를 기반으로 각 치료단계에 대한 위해도를 산출하였다. 프로세스 맵은 “외래 및 진료”와 “근접방사선 모의치료”, “CT 모의 치료”, “근접방사선치료계획”, “방사선치료”로 총 5단계로 구성하였으며, 각 치료단계를 세분화하여 세부단 계를 작성하였다. 위해도를 산출하기 위해 의사와 의학물리사, 선량설계사, 방사선사, 간호사가 참여하여 세부단계마다 발생빈도와 심각도, 불검출도를 평가하였다. 전반적으로 프로세스 맵은 각 치료단계마다 환 자 신원 확인 절차가 우선적으로 수행되며, 이는 다른 환자로 오인하여 서로 다른 치료계획이 수립되어 방 사선사고가 발생될 우려가 있다. 프로세스 맵을 기반으로 작성한 세부단계에 대해 위해도를 평가한 결과, 전반적으로 “외래 및 진료”와 “근접방사선치료계획” 과정이 높은 위해도로 평가되었다. 직종마다 평가한 위해도는 서로 다른 경향을 보였으며, 간호사는 방사선치료를 제외한 모든 과정이 55점 이상의 위해도를 보였으며, “근접방사선 모의치료” 과정이 88.8점으로 가장 높았다. 방사선치료를 수행하는 의료기관마다 치 료단계가 다소 차이가 있으므로 해당 기관에 대한 프로세스 맵을 작성하고 위해도를 산출하여 중점관리 항목을 집중적으로 리스크 관리가 수행되어야 할 것으로 생각된다.