제초제 저항성 유전자가 도입된 형질전환 토마토에서 T-DNA의 특성을 분석하였다. 형질전환 토마토의 T-DNA 도입형태를 분석하기 위한 primer쌍을 제작하여 rpPCR을 실시하 였다. T0-21(15:1, 4 copy), 26(3:1, 3 copy), 34(63:1, 4 copy)의 경우에는 5쌍의 primer를 이용하여 수행한 rpPCR 결과에서 양쪽이 절단된 불완전한 T-DNA 반복이 확인되었다. 도입된 T-DNA의 주변 서열 및 분포를 알아보기 위하여 총 52개체의 형질전환체에 대하여 TAIL-PCR을 실시하였다. 이 중 22개 형질전환체의 TAIL-PCR 산물의 염기서열을 분석하였는데, 2개의 형질전환체에서 나온 염기서열 결과는 토마토의 12번 염색체와 90%, 1개체는 토마토 clone 135006R과 98%, 5개체는 담배의 엽록체와 97% 상동성을 보였으며, 12개체는 다양한 vector의 염기서열을 나타내었고, 그 외 나머지 개체들은 토마토 유전체 정보와 는 유사성을 찾을 수 없었다.

Flammulina velutipes, amongst others known as Winter Mushroom or Enokitake is an important economic crop in Asia. The tetrapolarity (having four mating types) of this mushroom obligates mating and results in self-sterile progeny that carries unique genetic traits, making understanding of the genetic base desirable for breeding. Moreover, mating type genes are significant for evolutionary studies as their high polymorphism benefits phylogenetic comparisons. This polymorphism further makes mating type genes interesting candidates for genetic markers that allow identification of specific strains. Mating type loci in Agaricomycotina are classically termed A and B and control two different developmental pathways [for a review see 1]. They consist of tightly linked subloci that encode multiallelic genes. MatA loci contain two groups of divergently transcribed homeodomain proteins (HD1 and HD2) and heterodimerization of HD1 with a non-self HD2 protein forms a functional transcription factor that activates the A pathway. MatB loci hold pheromones and pheromone receptors. Pheromone genes encode small precursor proteins that are characterized by a C-terminal CAAX motif. Pheromone receptors typically contain 7 membrane spanning regions and are coupled to G-proteins. Binding of a pheromone to a receptor, triggers splicing of the (trimeric) G-protein, which activates the B pathway. New genetic data from recent genome sequences is challenging the strict concepts of old mating type models in fungi. MatB loci turn out to be rather diverse and contain considerable varying numbers of pheromone receptors and associated pheromones. To this, pheromone receptors which are not linked to matB loci have now been reported for C. cincerea, S. commune and L. bicolor [2, 3]. Also the organization of the matA locus is less strictly conserved than anticipated. Though far more tightly maintained than the matB locus, substantial differences in HD gene numbers and overall organization are reported [2, 3]. These differences stress the importance of determination of the individual mating type systems from industrially important mushrooms to assist breeding. Our analysis of F. velutipes strain 4019-20 uncovered 7 pheromone receptors together with 3 pheromones. The matB-3 locus of this strain however, is defined by only a single pheromone receptor and pheromone gene and our data strongly indicates that a 2nd pheromone receptor recently lost its function. The other receptor genes are non mating type specific. Finally, we detected three homeodomain genes distributed over two distant subloci. These subloci have been separated by two large inversions. Strikingly the distant matA subloci in S. commune seem to be separated by inversions as well. Synthenic mapping of a large regions from Coprinus cinerea, Laccaria bicolor, S. commune and F. velutipes shows that the matA loci originate from a single locus in a common ancestor of S. commune and F. velutipes that is represented by L. bicolor and C. cinerea. [1] U Kües. Micr Mol Biol Rev 64, 316 (2000) [2] H Niculita-Hirzel, J Labbé, A Kohler, F le Tacon, F Martin, IR Sanders and U Kües. New Phytol 180, 329 (2008). [3] RA Ohm, JF de Jong, LG Lugones, A aerts, E Kothe, JE Stajich, RP de Vries, E Record, A Levasseur, SE Baker, KA Bartholomew, PM Couthino, S Erdmann, TJ Fowler, AC Gathman, V Lombard, B Henrissat, N Knabe, U Kües, WW Lilly, E Lindquist, S Lucas, JK Magnuson, F Piumi, M Rausdaskoski, A Salamov, J Schmutz, FWMr Schwarze, PA van Kuyk, JS Horton, IV Grigoriev and HAB Wösten. Nat. Biotechnol ISSN: 1087-0156 (2010).

재배상황버섯의 유산균 증식 효과를 측정한 결과 B. breve에 대한 증식 효과와 배양배지의 pH 저하 효과가 우수했다. 사람 및 흰쥐의 장내 세균을 버섯추출물 함유 배지에서 배양시 배지의 pH 저하 효과가 우수할 뿐만 아니라 β-glucosidase의 효소 활성을 억제하였다. 그 외에도 대장암 및 방광암 등의 원인효소로 주목되고 있는 사람 및 흰쥐의 장내 세균층 효소인 β-glucosidase 및 tryptophanase 효소 활성을 억제하였다. 이외에도 인슐린 비의 존형 당뇨병의 치료제로 사용되는 acarbose보다는 약하지만 maltase, sucrase 및 알파아밀라제를 효과적으로 저해하였다.

In order to simulate a typhoon precisely, the satellite observation data has been assimilated using WRF (Weather Research and Forecasting model) three-Dimensional Variational (3DVAR) data assimilation system. The observation data used in 3DVAR was GPS Radio Occultation (GPS-RO) data which is loaded on Low-Earth Orbit (LEO) satellite. The refractivity of Earth is deduced by temperature, pressure, and water vapor. GPS-RO data can be obtained with this refractivity when the satellite passes the limb position with respect to its original orbit. In this paper, two typhoon cases were simulated to examine the characteristics of data assimilation. One had been occurred in the Western Pacific from 16 to 25 October, 2015, and the other had affected Korean Peninsula from 22 to 29 August, 2012. In the simulation results, the typhoon track between background (BGR) and assimilation (3DV) run were significantly different when the track appeared to be rapidly change. The surface wind speed showed large difference for the long forecasting time because the GPS-RO data contained much information in the upper level, and it took a time to impact on the surface wind. Along with the modified typhoon track, the differences in the horizontal distribution of accumulated rain rate was remarkable with the range of 600~500 mm. During 7 days, we estimated the characteristics between daily assimilated simulation (3DV) and initial time assimilation (3DV_7). Because 3DV_7 demonstrated the accurate track of typhoon and its meteorological variables, the differences in two experiments have found to be insignificant. Using observed rain rate data at 79 surface observatories, the statistical analysis has been carried on for the evaluation of quantitative improvement. Although all experiments showed underestimated rain amount because of low model resolution (27 km), the reduced Mean Bias and Root-Mean-Square Error were found to be 2.92 mm and 4.53 mm, respectively.

To predict annual energy production (AEP) accurately in the wind farm where located in Seongsan, Jeju Island, Equivalent wind speed (EQ) which can consider vertical wind shear well than Hub height wind speed (HB) is calculated. AEP is produced by CFD model WindSim from National wind resource map. EQ shows a tendency to be underestimated about 2.7% (0.21 m/s) than HB. The difference becomes to be large at nighttime when wind shear is large. EQ can be also affected by atmospheric stability so that is classified by wind shear exponent (). AEP is increased by 11% when atmosphere becomes to be stabilized ( > 0.2) than it is convective ( < 0.1). However, it is found that extreme wind shear ( > 0.3) is hazardous for power generation. This results represent that AEP calculated by EQ can provide improved accuracy to short-term wind power forecast and wind resource assessment.

Purpose – This study investigated the effects of leaders of national universities on faculty creativity. The study examined transformational leadership and creativity theories and investigated self-efficacy, the parameter between transformational leadership and creativity, to examine its moderating effect and provide policy implications for national universities. Research design, data, and methodology – This study built four hypotheses based on the literature review, and tested these using a survey methodology. The collected data were analyzed using SPSS 15.0. Result – At the national universities, the results were as follows: charisma of leaders had a positive influence on member creativity; intellectual stimulus of leaders had a positive influence on member creativity; and, individual consideration of leaders had a positive influence on member creativity. Therefore, leaders’ transformational leadership had a positive influence on member creativity. Conclusion – Creativity is thought to be important for organizational survival and continuous development at rapidly changing education administrations. Educational administration leaders should exercise transformational leadership to develop member creativity. Member self-efficacy, which had a mediating effect on creativity, requires leadership to develop it.

In order to clarify the relation between sea breeze penetration and Planetary Boundary Layer development in southeastern part of the Korean Peninsula, several numerical assessments were carried out using atmospheric numerical model WRF(Weather Research and Forecasting). Compared with onset time of sea breeze at eastern coast area(Uljin), the time at southern coast region(Masan) with complex costal line tend to delay for several hours. The penetration patterns of sea breeze between two coastal regions are some different due to the shape of their coastal line and back ground topography. Intensified valley wind due to high topography of lee side of Uljin can help penetration of sea breeze at early time. So penetration of sea breeze at early time often prevent PBL to develop at Uljin and lower PBL height last for a day time. But because of late penetration of sea breeze at Masna, PBL Height dramatically decrease after 1500LST. The distribution of front genesis function based on the heat and momentum variation are explained obviously the sea breeze penetration patterns and agreed well with the PBL height distribution.

Wind power energy is one of the favorable and fast growing renewable energies. It is most important for exact analysis of wind to evaluate and forecast the wind power energy. The purpose of this study is to improve the performance of numerical atmospheric model by data assimilation over a complex coastal area. The benefit of the profiler is its high temporal resolution and dense observation data at the lower troposphere. Three wind profiler sites used in this study are inhomogeneously situated near south-western coastal area of Korean Peninsula. The method of the data assimilation for using the profiler to the model simulation is the three-dimensional variational data assimilation (3DVAR). The experiment of two cases, with/without assimilation, were conducted for how to effect on model results with wind profiler data. It was found that the assimilated case shows the more reasonable results than the other case compared with vertical observation and surface Automatic Weather Station(AWS) data. Although the effect of sonde data was better than profiler at a higher altitude, the profiler data improves the model performance at lower atmosphere. Comparison with the results of 4 June and 5 June suggests that the efficiency with hourly assimilated profiler data is strongly influenced by synoptic conditions. The reduction rate of Normalized Mean Error(NME), mean bias normalized by averaged wind speed of observation, on 4 June was 28% which was larger than 13% of 5 June. In order to examine the difference in wind power energy, the wind power density(WPD) was calculated and compared.