Characteristics and useful effects for human health of antioxidant carbon nanodots contained in microwave-assisted Opuntia humifusa extract (MA-OHE(CD)) were investigated in this work. MA-OHE(CD) was characterized using transmission electron microscopy, dynamic light scattering analysis, X-ray diffraction, UV–vis spectroscopy, X-ray photoelectron spectroscopy, and Fourier transform-infrared spectroscopy. Besides, total phenolic content and antioxidant activity of MA-OHE(CD) were measured. It was revealed that the MA-OHE(CD) increases aquaporin-3 expression in human epidermal keratinocytes cell with hydrophilic characteristics. Moreover, the bio-active compound extraction efficacy and antioxidant activity of microwave-assisted extraction were great when compared to maceration.

Pine Wilt Disease (PWD) is a disease causing mass deaths of pine trees in South Korea, and the dead trees serve as breeding grounds for insect vectors responsible for spreading the disease to other host trees. Because the PWD requires early monitoring to minimize its damage on domestic forestry, this study aims to develop a species distribution model for predicting the potential distribution of PWD by using artificial neural network (ANN) with time-series data. Among the architectures, the Convolutional Neural Network exhibited the highest performance, achieving a validation accuracy of 0.854 and a cross-entropy loss of 0.401, and the InceptionTime model emerged as the second-best performer. This study identified the best-performing ANN architecture for a spatiotemporal evaluation of PWD occurrence, emphasizing the importance for determining hyperparameters with ecological characteristics and data types to apply deep learning into SDMs.

2022년 국내 노지 마늘, 대파, 양파, 부추 작물재배지에서 채집한 파총채벌레 지역집단들에 대하여 살충제 저항성을 조사하였다. 제조사 추천약량에서의 살충력은 acrinathrin SC를 제외한 6종 약제들이 안성 등 8개 집단 에서 모두 90%이상을 보였으며, Spinetoram SC와 fluxametamide EC는 추천농도의 100배 희석농도에서도 전 지역 집단에 걸쳐 높은 살충력을 보여주었다. 미리 저항성 진단농도로 코팅한 바이알을 이용한 지역집단의 저항성 검정을 실시한 결과, emamectin benzoate의 저항성이 신안 등 9개 지역집단에서 매우 높았으며, chrantraniliprole은 부산 등 4개 , spinetoram은 의성 등 3개, actamiprid와 chlorfenapyr는 각각 1개의 지역집단에서 저항성이 높게 발달 하고 있는 것을 확인할 수 있었으며, 지역별로는 주요 대파 및 양파 재배지인 안성, 서산, 진도, 신안 지역의 저항성 이 모든 약제에 대하여 전반적으로 높게 나타났다.

Apolygus is one of speciose genus in subfamily Mirinae, including more than 80 species worldwide. Most of Apolygus species are phytophagous, and some species are regard as severe crop pest. For that, precise diagnosis and classification for this genus is crucial. In this work, we provide checklist of Korean Apolygus species with a new species and new distributional records. Diagnostic characters of the new species are discussed with the illustrations of adult male, female specimen and genitalic structures. A key to the Korean Apolygus is also presented.

Anoplophora horsfieldii (Hope, 1843), 국명 노랑알락하늘소(가칭)는 아열대 지역에 주로 분포하는 종으로 2019 년에 제주도 용연계곡에서 처음 발견되었으며, 2023년에 국내 정착이 보고되었다. 국내에서는 팽나무에서 발생 하는 것으로 알려졌으나, 섭식방식, 산란 선호성 등 생태적 특성에 대해서는 국내외 연구가 전무하다. 따라서 본 연구에서는 노랑알락하늘소의 기초적인 생태정보를 제공하고자 실내 산란 선호성 조사 및 야외조사를 진행 했다. 산란 선호성 실험은 기주식물로 알려진 팽나무, 멀구슬나무와 제주도내 대표 수종인 동백나무, 종가시나무 을 대상으로 했다. 이 중 팽나무에서만 산란이 확인었으며, 상대적으로 굵은 팽나무에서 더 많은 산란이 확인되었 다. 야외조사에서는 최대 발생지인 용연계곡을 기준으로 반경 3 km 내에서 노랑알락하늘소의 탈출공이 확인된 32 그루의 팽나무를 대상으로 피해를 조사하였다. 나무의 굵기는 흉고직경이 20-40 cm인 팽나무에서 탈출공이 많이 확인되었고, 높이는 지면으로 부터 0-100 cm 구간에서 가장 많은 탈출공이 발견되었다.

The aphid genus Tuberaphis Takahashi belongs to the tribe Cerataphdini (Hemiptera: Hormaphidinae) and comprises 17 valid species. Generally, its species are distributed in East and South East Asia, where they primarily feed on Styrax spp. (Styracaceae) and utilize species within the Loranthaceae family as their secondary hosts. They form various shapes of galls on Styrax, looking coral and broccoli etc. Currently, the only aphid species reported to exclusively produce gall forms resembling broccoli head-shaped is Tuberaphis takeouchii (Takahashi). For the first time in Korea, an aphid species forming gall structures with a broccoli-head-like appearance has been discovered. We confirmed that the species discovered in Korea is a new species through morphological and molecular comparisons with T. takenouchii (Takahashi). To comprehend the life cycle of this new species, we conducted a year-long study investigating how the gall forms change and examined aphid polymorphism across different seasons. The results of this study additionally include as followings: 1) Species identification, 2) DNA barcoding, 3) population genetic analyses, and 4) morphological comparison using SEM

Miridae show remarkable diversity (>11,700 spp.), accounting for a quarter of all Heteroptera. However, their phylogeny and evolutional history is still remain unclear. In this work, we provide new suggestions for the phylogeny of Miridae using a larger dataset than previous studies. In addition, we suggest an alternative evolutionary history based on newly calibrated divergence dates for Miridae and its subordinate groups, and present probable factors of the family’s success in terms of species diversity. The entire dataset comprises 16 outgroups and 188 ingroup taxa including all seven known subfamilies and 37 out of 45 known tribes. Each species is aligned as 3,577 bp with six molecular loci (COI, 16S rRNA, 18S rRNA, 28S rRNA D3 region, H2A, and H3A).

Honey bees are crucial pollinators for agricultural and natural ecosystems, but are experiencing heavy mortality in Korea due to a complex suite of factors. Extreme winter losses of honey bee colonies are a major threat to beekeeping but the combinations of factors underlying colony loss remain debatable. Finding solutions involves knowing the factors associated with high loss rates. To investigate whether loss rates are related to Varroa control and climate condition, we surveyed beekeepers in korea after wintering (2021–2022 to 2022–2023). The results show an average colony loss rate of 46%(2022) and 17%(2023), but over 40% colony loss before wintering at 2022. Beekeepers attempt to manage their honey bee colonies in ways that optimize colony health. Disentangling the impact of management from other variables affecting colony health is complicated by the diversity of practices used and difficulties handling typically complex and incomplete observational datasets. We propose a method to 1) Varroa mite population Control by several methods , and 2) Many nursing bee put in hive before wintering.

This study investigated ethopabate (EPB) residues in edible tissues of broiler chickens given in drinking water and established the withdrawal time (WT) of EPB in poultry tissues. Twenty-four healthy Ross broiler chickens were orally administered with EPB at the concentration of 3.8 mg/L for 14 days (EPB-1, n=24) and 15.2 mg/L for 7 days (EPB-2, n=24) through drinking water, respectively. After the drug treatment, tissue samples were collected from six broiler chickens at 0, 1, 3, and 5 days, respectively. EPB residue concentrations in poultry tissues were determined using LC-MS/MS. Correlation coefficient values ranged from 0.9980 to 0.9998, and the limits of detection and quantification (LOQ) were 0.03~0.09 and 0.1~0.3 μg/kg, respectively. Mean recoveries in muscle, liver, kidney and skin/fat tissues were 95.9~109.8, 108.7~115.3, 89.9~96.6 and 86.7~96.8%, respectively, and coefficient of variations were less than 17.11%. At the end of the drug-administration period (0 day), EPB was detected at levels under the LOQ in all tissues from both the EPB-1 and EPB-2 groups. According to the results of EPB residue in Ross broiler tissues, withdrawal periods of both EPB-1 and EPB-2 in poultry tissues were established to 0 day. In conclusion, the developed analytical method is suitable for the detection of EPB in poultry tissues, and the estimated WT of EPB in poultry tissues will contribute to ensuring the safety of Ross broiler chickens.

Background: Pluripotent stem cells (PSCs) including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) offer the immense therapeutic potential in stem cell-based therapy of degenerative disorders. However, clinical trials of human ESCs cause heavy ethical concerns. With the derivation of iPSCs established by reprogramming from adult somatic cells through the transgenic expression of transcription factors, this problems would be able to overcome. In the present study, we tried to differentiate porcine iPSCs (piPSCs) into endothelial cells (ECs) for stem cell-based therapy of vascular diseases. Methods: piPSCs (OSKMNL) were induced to differentiation into ECs in four differentiation media (APEL-2, APEL-2 + 50 ng/mL of VEGF, EBM-2, EBM-2 + 50 ng/ mL of VEGF) on cultured plates coated with matrigel® (1:40 dilution with DMEM/F-12 medium) for 8 days. Differentiation efficiency of these cells were exanimated using qRT-PCR, Immunocytochemistry, Western blotting and FACS. Results: As results, expressions of pluripotency-associated markers (OCT-3/4, SOX2 and NANOG) were higher observed in all porcine differentiated cells derived from piPSCs (OSKMNL) cultured in four differentiation media than piPSCs as the control, whereas endothelial-associated marker (CD-31) in the differentiated cells was not expressed. Conclusions: It can be seen that piPSCs (OSKMNL) were not suitable to differentiate into ECs in the four differentiation media unlike porcine epiblast stem cells (pEpiSCs). Therefore, it would be required to establish a suitable PSCs for differentiating into ECs for the treatment of cardiovascular diseases.

Synced advertising (SA) has grown in popularity due to its ability to facilitate multitasking and personalize ads to the media content users consume. Research on the effects of SA has been limited, but it has been suggested that repetition and personalization with SA will lead to a stronger, lasting impression. However, there are concerns that SA could be perceived as intrusive and lead to privacy issues. This study seeks to explore how the consumer paradox between perceived relevance and privacy concerns may affect the outcome of the advertisements.

This study examines the impact of others' reviews (reviews, product ratings) on consumer responses (helpfulness & buying intention) in an online shopping platform. We propose that review features, such as review message construal and review inconsistency between review message valence and rating, determine review credibility as product-related information, which in turn influences helpfulness of review and buying intention toward the product. Specifically, low- level construal review messages will be perceived as more credible than high-level construal review messages, which affect helpfulness and buying intention. In addition, the effect of review message construal will be moderated by review inconsistency. The effect of the review message construal will be enhanced in the condition of review consistency (positive content-high rating & negative content-low rating), but it will be disappeared or attenuated in the condition of inconsistency (positive content-low rating, negative content-high rating).

본 연구는 아시아의 국제관계가 변화하는 맥락에서 "아시아 나토"의 실 행 가능성과 잠재적 영향을 검토한다. 아시아 버전 나토의 가능성을 분 석하기 위해 세 가지 목표를 추구한다: (1) 나토의 성격을 조사하고 아시 아에서의 잠재적 적용을 검토한다, (2) 세력균형이론과 위협균형이론의 관점에서 나토의 과거 실패를 검토한다, (3) 정치와 군사적 도전을 고려 한 아시아 나토의 실행 가능성을 평가한다. 이 연구는 미국과 일본을 중 심으로 아시아 나토에 대한 관심이 증가한 지정학적 변화를 탐구한다. 현재 미중 균형과 위협 사이의 균형은 양자 및 삼자 동맹을 설정하는 데 이르렀지만 아시아 나토의 가능성은 여전히 쉽지 않다. 그러나 중국이 지역 패권 추구로 인한 압도적인 외부 위협을 초래하면, 아세안 국가들 은 집단 안보 체제에 가입할 수 있다. 본 논문은 특히 이러한 상황에서 한국이 직면할 수 있는 문제점에 대해서도 지적한다.

The Body Appreciation Scale-2 (BAS-2) measures the extent to which individuals feel, accept, and respond to their own bodies in a positive manner. Given the research need to explore positive body image and its associations with various sociocultural factors and related consequences among individuals with various cultural backgrounds, several studies have established the psychometric properties and factor structures of the BAS-2 in different languages and samples with different characteristics. The current study investigated the psychometric properties and measurement invariance of a Korean version of the BAS-2 in an older Korean adult population (599 older Korean adults with the average age of 70 years). Data were collected using both online and offline (paper-based) survey questionnaires. The results of exploratory factor analyses and confirmatory factor analysis evidenced the unidimensional factor structure and measurement invariance of the Korean BAS-2 among older Korean men and women, after dropping item 1. Scalar invariance was supported across gender, and men and women did not significantly differ in observed mean scores of the Korean BAS-2. The results also supported good convergent validity and criterion validity. Incremental validity was demonstrated by predicting self-esteem over and above measures of age, BMI, subjective financial and health status, body esteem, and ageism. High internal reliability and test-retest reliability over a 2-week period were confirmed. Overall, the results of this study support the reliable use of a Korean BAS-2 to measure positive body image among older Koreans after excluding item 1.