In this study, Bacillus sp. SRCM 112835 was isolated from soybean paste (Doenjang, Korean Fermented Soy Paste). Bacillus sp. SRCM 112835 showed biosorption of the Cu (II) in aqueous solution. The strain effectively absorbed 30.2% of the Cu (II) from a 52.3 mg/L within 60 min. The properties of the Bacillus sp. SRCM 112835 were investigated by Fourier transform infrared spectroscopy (FT-IR), point of zero charge (pHpzc), and phylogenetic analysis. The influence of initial pH (2.08-9.98) and biomass dosage (0.005-0.07 g) were likewise probed. Isotherm and kinetic experiment results suggested that the Langmuir isotherm and pseudo-second-order kinetic models well-fitted the experimental data, respectively.

The present study investigated the effect of enzyme inclusion on silage quality using meta-analysis tool. A total of 16 research papers reporting the effect of enzyme application on silage quality were employed in the meta-analysis of this study. Mixed model for integrating quantitative results from multiple studies was used first to calculate the predicted error of each study. Individual error from the estimated model was the applied into standard deviation of each study to calculate the mean difference. Finally, summary effect was determined using standard mean difference (SMD) and inversed variance weighting. Mixed model analysis and SMD analysis showed the same effect patterns in all analysis items. Enzyme inclusion in silage significantly (p < 0.05) altered all silage quality characteristics investigated compared to control when enzyme was not included. Our results showed that enzyme treatment increased dry matter content, preserved crude protein effectively, and elevated water soluble carbohydrate content. However, the pH value, acetic acid, propionic acid, neutral detergent fiber, and acid detergent fiber contents in silage with enzyme inclusion were lower than those of the control.

The objective of this study was to determine the effect of bacterial inoculation (Lactobacillus plantarum or combo inoculant mixed with Lactobacillus plantarum and Lactobacillus buchneri) and addition of fibrolytic enzyme on chemical compositions and fermentation characteristics of whole crop barley (WCB) and triticale (TRT) silage, their ruminal in vitro fermentation, and digestibility. In TRT silage, enzyme addition significantly (p<0.01) decreased NDF content compared to no enzyme addition treatment. Organic acids such as lactate and acetate contents in WCB and TRT silages were significantly (p<0.01) higher compared to those in the control. Particularly, lactate content was the highest in L. plantarum treatment. Fibrolytic enzyme treatment on both silages had relatively higher lactic acid bacteria content, while mold content was lower in both treatments compared to that in the control. In vitro dry matter digestibility was generally improved in WCB silages. It was higher (p<0.01) in TRT with mixed treatment of L. plantarum, L. buchneri, and enzyme compared to others. In vitro ruminal acetate production was relatively higher in treatments with both enzyme and inoculant additions compared to that in the control. Therefore, the quality of silage and rumen fermentation could be improved by inoculants (L. plantarum and L. buchneri) regardless whether whole crop barley (WCB) or triticale (TRT) silage was used. Although it was found that fibrolytic enzyme addition to both silages had various quality and rumen fermentation values, further study is needed

The aim of this study was to evaluate the alleviative effects of a nude pack containing black tea water extract (NPBT) on inflammation and skin barrier damage in hairless mice. Skin inflammation and skin barrier damage were induced by UVB irradiation to the backs of hairless mice for five weeks. And, at the same time, saline (C), NPBT (1%: E1, 2%: E2), and 0.01% retinoic acid diluted with polyethylene glycol (PC) were applied topically twice per day, five days per week, for a period of five weeks. The skin erythema indices of the E1 and E2 groups were significantly lower (p<0.05) than those of the C group through one week after the beginning of the experiment. Meanwhile, water contents of the E1 and E2 groups were significantly higher (p<0.05) than those of the C group through two weeks after the beginning of the experiment. Remarkable thickening of epidermis and dermis was observed in the C group, compared with the PC, E1, or E2 groups. In the C group, infiltration of many inflammatory cells, including neutrophils and lymphocytes, was observed in dermis and a large number of mast cells were observed in dermis and hypodermis, and the degree of degranulation was remarkable. However, these phenomena were alleviated in the PC, E1, and E2 groups. Therefore, NPBT could have considerable inhibitory efficacy on inflammation and skin barrier damage induced by UVB irradiation.

본 연구에서는 다양한 식품유해미생물에 대한 길항작용 및 항산화활성이 우수한 B. subtilis SRCM102046 균주를 식품보존 소재로서 이용하기 위해 반응표면분석법을 이용하여 균체 증량을 통한 항균활성 및 항산화활성 증대를 도모하고자 하였다. SRCM102046의 산업적 활용을 위한 성장조건을 최적화하기 위해 배양시간에 따른 균체 성장을 조사하였으며, 통계학적 기법인 반응표면분석법을 사용하였다. SRCM102046의 최적 성장을 위한 배지 성분을 선별하기 위해 Plackett-Burman design을 이용하였으며, PBD 결과 선별된 배지 성분으로 molasses, sucrose, peptone으로 예측되었다. 각 배지 성분의 최적농도를 결정하기 위한 방법으로 central composite design을 이용하였으며, 최종적으로 예측된 각 배지 성분의 농도는 molasses 7 g/L, sucrose 7 g/L, peptone 2 g/L로 예측되었다. 이때의 균체량은 22.03±1.30 g/L로 예측되었으며, 통계분석을 통해 실험모델의 적합성을 확인하였다. 또한, 실험 모델을 수행하여 건조균체량을 측정한 결과 22.02±0.35 g/L로 측정되어 실험모델에 의해 예측된 값이 오차범위 내에 존재하여 모델의 신뢰성이 매우 높음을 확인하였으며, 이는 실험모델에 의해 예측된 최적배지 사용시 최적화 이전의 LB 배지에서의 균체량(2.47±0.03 g/L)대비 약 9배의 균체량이 증가함을 확인하였다. 또한 최적배지에서 B. subtilis SRCM102046 배양 시 항균활성은 대조구로 사용된 LB 배지에서의 항균활성 대비 최대 140% 향상되었으며, 항산화활성은 약 100.41% 증가함을 확인하였다. 본 연구를 통하여 식품보존제로서B. subtilis SRCM102046의 산업화를 위한 배지최적화를 수행하였으며, 추후 박테리오신의 정제 및 특성 등에 대한 세부적인 연구가 필요하지만 본 연구를 통해 확립된 배양조건을 기반으로 식품보존 소재의 측면에서 매우 유용한 자료로 활용될 수 있을 것으로 기대된다.