Seeds of some barberry species have embryo with physiological dormancy that requires a cold stratification for germination. Berberis amurensis Rupr. is a native species of Japan, Korea, the Russian far east, and parts of China. This specific plant is important for its edible fruits and rhizomes with high medicine value. This study aimed to determine the effect of stratification on germination and physiological change of B. amurensis. Seeds were placed on sterilized sand medium moistened with distilled water in 9 cm diameter petri dishes and stored at 4 and 25˚C for 0, 15, 30, 45 and 60 days. Each treatment had 40 seeds per replica, and three repetitions per treatment. Immediately after stratification, total phenolics contents (TPC) was analyzed and seeds incubated at 15/6˚C for 12 weeks. Warm stratification had a significant effect on seed forcing for germination than cold stratification treatment. At 25˚C for 60 days, stratified seed showed highest germination percentage (25.7±4.3%) and germination started in 14 days of the stored period. Whereas TPC was significantly decreased with increasing stored period. Contrastively, cold stratification had no effect on the germination ability. In the same way germination percentage of non-stratified seeds were also zero. The results confirmed that B. amurensis seeds were in a dormant state and warm stratification increased the germination ability by breaking of dormancy.

Islands often have relatively well-preserved ecosystem and an abundance of bioresources with a high conservation value, with unrecorded species continuing to be reported (Hong, 2011). Approximately 1,000,000 species of insect known worldwide (Costello et al., 2012), and 20,710 species are known in Korea (NIBR, 2023). Among these, there are 6,117 species in Korean islands (HNIBR, 2022). Native insect Bio-scan project for Korean islands is to estimate the number of insect species on Korean islands. We attempted to estimate the number of insect species on Korean islands using Barcode Index Number (BIN), and also found unrecorded species. The samples were collected four times from April to July at five locations in the Amtedo, an island located in Shinan-gun Jeollanam-do. We tried to obtain a minimum of one to usually a maximum of four samples per morphospecies to enable DNA barcoding.

To investigate the impact of forestry projects on the carbon stocks of forests, we estimated the carbon stock change of above-ground and soil before and after forestry projects using forest type maps, forestry project information, and soil information. First, we selected six map sheet with large areas and declining age class based on forest type map information. Then, we collected data such as forest type maps, growth coefficients, soil organic matter content, and soil bulk density of the estimated areas to calculate forest carbon storage. As a result, forest carbon stocks decreased by about 34.1~70.0% after forestry projects at all sites. In addition, compared to reference studies, domestic forest soils store less carbon than the above-ground, so it is judged that domestic forest soils have great potential to store more carbon and strategies to increase carbon storage are needed. It was estimated that the amount of carbon stored before forestry projects is about 1.5 times more than after forestry projects. The study estimated that it takes about 27 years for forests to recover to their pre-thinning carbon stocks following forestry projects. Since it takes a long time for forests to recover to their original carbon stocks once their carbon stocks are reduced by physical damage, it is necessary to plan to preserve them as much as possible, especially for highly conservative forests, so that they can maintain their carbon storage function.