국내 유통 중인 건조 목이 8건(국내산 3건, 중국산 5건) 을 수집하여 잔류농약 321종, 중금속 7종, 방사능 3종의 유해물질 잔류성분을 조사하였다. 잔류농약 검사결과 국내산 3건에서는 검출되지 않았으나, 중국산 5건 중 4건에서 Chlorpyrifos, Isoprocarb, Mepiquat chloride, Carbendazim이 검출되었다. 농산물 농약 잔류허용기준에 의하면 목이는 Mepiquat chloride (0.5 mg/kg 이하)만 기준치가 설정되어 있다. 또한 중금속 검사결과는 모든 건조 목이에서 미량의 중금속이 검출되었으나, 기준치 이하로 낮게 나타났다. 다만 국내산 시료 1건에서 납 함량이 기준치(0.3 mg/kg)보다 높게 나타나 정확한 원인 규명을 위해서는 톱밥배지, 지 하수, 보관·유통 과정에 대한 추가적인 조사가 필요할 것 으로 보여진다. 방사능 조사결과에서는 모든 건조 목이에서 방사능 농도가 MDA값 이하로 불검출 수준으로 나타났다. 국내산과 중국산의 평균 방사능 농도는 요오드( 131 I)와 세슘 ( 134 Cs)은 비슷한 수준으로 나타났으며, 세슘( 137 Cs)은 국내산보다 중국산에서 다소 높게 나타났다. 방사성 세슘 ( 137 Cs)은 버섯류에 잘 농축되는 것으로 알려져 있는데 이번 조사에서는 국내 유통 중인 건조 목이는 방사능에 안전하게 나타났다.
Lentinula edodes, a member of the Pleurotaceae, is a blood pressure and cholesterol lowering effect. Recently, research the reduction of irritation and the enhancement of function have been cultivation of natural resources using mushroom mycelium. For the development of food fermented with L. edodes mycelium of natural medicinal herb remnants, determined to be 20%, and the product were used after lyophilization. In this study, we were analyzed the contents of β-glucan and amino acids of fermented herb remnants by L. edoeds mycelials. β -Glucan is a type of polysaccharide that has immune-enhancing and is known to inhibit cancer cells. The content of β-glucan and total amino acids of fermented herb remnants by L. edoeds mycelials were 35.13% and 8,697.36 mg%, respectively. The content of essential amino acid from fermented herb remnants by L. edoeds mycelials was 3,226.85 mg%. Collectively, our findings suggest that fermented herb remnants by L. edoeds mycelials extract could be used as an chemo-preventive material for consumers.
Polygonum multiflorum (Polygonaceae family) is effective to cholesterol lowering effect, increased immunity effect and whitening effect. P. multiflorum root is used for medicinal herb that can be used as raw material for food. In this study, acetic acid fermentation was adding extracts of P. multiflorum root(0%, 1%, 3%, 5%) for the developing healthy beverage. In this study, we investigated the contents of free sugars, organic acids and inorganic components of P. multiflorum root. As a result of free sugars from P. multiflorum root analysis, three kind of free sugars were determined the glucose, sucrose and lactose by HPLC. Four kind of organic acids were found in lactic acid fermented P. multiflorum root. And oxalic acid, lactic acid and succinic acid were detected in organic acids. The content of lactose and lactic acid in lactic acid fermented P. multiflorum root was the highest among the free sugars and organic acids. The content of minerals were in high orders of sodium> calcium> magnesium in P. multiflorum by acetic acid fermentation. The content of inorganic components was highest in lactic acid fermented solution with 5% and proportional to the content of P. multiflorum
Schizophyllum commune belongs to Schizophyllum, Schizophyllaceae, Agaricales, Agaricomycetes and Basidiomycotatina. The morphological shape of S. commune was similar with the cap, fan and hair. As for the sensory evaluation, chewness was not good for human's sensory. The major useful component was identified schizophyllan from S. commune extract. Schizophyllan has a great potential for developing medical supplies and functional foods. In our study, we carried out to internal transcribed spacer regions(ITS) for the specimens systematically. Also, we found out the optimal growth conditions of different temperature and media for S. commune cultivation. As the results of these test showed that when we compare with PDA, MCM and ME1 medium, we confirm that optimal temperature is 35℃ in MCM and ME1 medium. And optimal media of growth condition is ME1 and MCM.
A Lentinula edodes has abundant nutritious components such as a fiber, minerals and vitamins. It also was known to effective of anticancer and the immune enhancement. However, studies of mushroom-re1ated products were not still enough. Mushroom jerky was examined by using the under grade Lentinula edodes in this study. Content of stipe was most appropriate 24-30% in slices of dried mushroom. A grinder used 2mm sieve, and graund three or four times. A mushroom and spices were boiled to 30min in order to control the microbe occurrence. A mushroom, water, spices were mixed at ratio of 1 : 4: 1.5 and boiled 10 minutes for sterilization. The optimum drying temperature was 60~70℃ but the edge of slice was burned 80~90℃. Dry time was most appropriate for 4~5 hours.
A Lentinula edodes was known to the excellent foods that abundant in nutritious components such as a fiber, minerals and vitamins. Fermentation conditions for rice wine were examined by using the medicinal herbs and the incuvated-rice with mycelia of Lentinula edodes. The average of ethanol contents was in the range of 3.4~4.1% at initial fermentation, increasing up to 16.9% after 15 days. Total acid, pH, reducing sugar and alcohol content were similar to the control during storage periods. The change of colors was measured by Hunter's color value during fermentation. The L value was the highest on 15th day of fermentation.
Aluminum (Al) toxicity in plants is one of the major limitations to crop growth on acid soils. The Al-induced change of H+-ATPase expression has been regarded as an important mechanism for Al tolerance in soybean. To investigate whether translational regulation of plasma membrane H+-ATPase is involved in the response to Al stress, we conducted western - blot of this protein. The results show that western - blot of plasma membrane H+-ATPase in the "Sowon" (Al tolernace) significantly increased in translational expression level, while citric acid (50 μM) with Al (50 μM) treatment has not effected. In contrast, Al sensitive cultivar "Poongsannamool" inhibited expression level of plasma membrane H+-ATPase with Al treatment. Two - dimensional gel analysis were performed to determine the protein induction patterns of control and Al (50 μM, 24 h) treated soybean. There are many changes of plasma membrane proteins in both cultivars under Al stress. Especially "Sowon" was significantly enhanced the expression of the plasma membrane H+-ATPase in Al treatment. But protein expression of "Poongsannamool" was less than "Sowon". These results suggest that the regulatory role of plasma membrane H+-ATPase may involved the tolerance mechanism in soybean roots. At the present, transcriptional level of H+-ATPase is under investigation.