청도반시 추출물로부터 라디칼 소거활성, T-bet promoter 활성, IL-4 발현 활성의 상관관계를 분석하고자 7월부터 10월까지 감을 시기별로 수확하여 분자염증 활성 여부를 확인하였다. 감의 무게는 시기가 경과할수록 무거워지면서 항산화 활성에 있어서는 감이 미숙과일수록 항산화활성이 우수하였다. 4가지의 용매로 추출한 감의 항산화 활성도 유사한 양상을 보였다. T-bet promoter 활성은 추숙이 될 수록 억제되는 양상을 보였는데, 이에

CA저장에 적합한 떫은감 품종을 선발하기 위해 청도반시, 사곡시, 고종시, 봉옥시 4품종을 공시하여 농도를 12, 16%로 하였으며 농도는 같이 3%로 하여서 약 160일간 저장하여 품질변화를 조사한 결과는 다음과 같다. 가용성 고형분 함량은 저장기간이 경과함에 따라 거의 변화가 없거나 감소하였으며, 청도반시, 사곡시, 고종시는 12%, 16% 모두 일반 저온저장고와 비슷한 수준이었으나, 봉옥시는 고농도의 로 가용성 고형분의 감소가 현저하였다.

In our studies on the role of -galactosidase in fruit softening, significant difficulty, was encountered in our attempts to extract RNA from persimmon(Diospyros kaki L. cv. Fuyu) fruit due to astringency and tannin content. Initial, unsuccessful RNA extractions involved methods using guanidinium isothiocyanate/CsCl with and without polyvinylpyrrolidone(PVP), phenol/sodium lauryl sulfate(SDS), guanidinium hydrochloride, as well as polysomal RNA purification method that used 0.2 M Tris-HCI (pH 9.0) containing KCI, Mg-acetate, EDTA, -mercaptoethanol, and sucrose. A method was devised which employed treatment of fruit with CO2 gas to diminish astringency prior to RNA extraction, followed by extraction of tissue powders with Proteinase K extraction buffer containing PVP and ascorbate at an alkaline pH. This procedure resulted in the removal of tannins and other polyphenolics and extraction of relatively large amount of high-quality RNA suitable for cDNA library construction and polymerase chain reaction(PCR). Futhermore, the procedure does not use the toxic and corrosive chemical guanidinium isothiocyanate or require ultracentrifugation.

Ethylene was treated or inhibited to investigate its effect on the physiological changes related to induction of flesh browning in Fuyu persimmon fruit. The response of fruit to ethylene was so slight, that the Fuyu fruit seemed to possess a similar characteristic to non-climacteric fruit. The flesh browning was however enhanced by ethylene treatment, although any significant increment of phenolic content or PPO activity in flesh tissue was not detected. Ethylene induced not only increasing of ion leakage from fruit tissue, but the fatty acids extracted from ethylene-treated fruit tissue were also more saturated. It was suggested that ethylene be related in the changing of membrane permeablity via saturating of fatty acid in membrane lipid. That could result in increased leakage of vacuole-stored phenolic compounds, which oxidized further by PPO to cause fruit flesh to brown.

This paper was carried out to investigate changes in chromatograms of polysacctatides and soluble pectins on Sephadex G-50 and non-cellulosic neutral sugars of polysaccharides isolated from cell wall of persimmon fruits treated with polygalacturonase and -galactosidase in vitro. The chromatogram pattern of soluble pectins extracted from cell wall treated with -galactosidase on Sephacryl S-500 column were similar to those of untreatment, but contents of soluble pectins treated with -galactosidase were different from those of untreatment. The patterns of chromatograms In soluble pectins extracted from cell wall treated with polygalacturonase were more complex and lower molecular polymer than those of other cell wall-degrading enzyme treatments. Non-cellulosic neutral sugar of polysaccharides in fraction I of soluble material treated with polygalacturonase was rhamnose, those in fraction II were similar to those in fraction III and contents of arabinose, xylose and glucose were higher than contents of other non-cellulosic neutral sugars. Non-cellulosic neutral sugars of polysaccharides in fraction I in soluble material by -galactosidase treatment were rhamnose, arabinose, galactose and mannose. Content of glucose of polysaccharides in fraction II was higher than that in fraction I . Non-cellulosic neutral sugars treated with mixed enzyme were rhamnose, fucose, arabinose, xylose, mannose, galactose and glucose. Compositions of non-cellulosic neutral sugars of polysaccharides in fraction I were similar to those in fraction II and III.