Covalent modifications of histone tails have fundamental roles in chromatin structure and transcriptional activity of a target locus. One of such modifications, Methylation at Lysine 9 of histone H3 (H3-K9) causes several epigenetic phenomena including heterochromatin formation, transcriptional regulation and DNA methylation. Setdb1, H3-K9 specific histone methyltransferase, functions in gene silencing, heterochromatin formation and essential role for early development. Here, we demonstrate that Setdb1 associates with promyelocytic leukemia (Pml) protein from the early stage of mouse development and is a constitutive member of PML nuclear bodies (PML-NBs) that have been linked to many cellular processes such as apoptosis, DNA damage responses, and transcriptional regulation. Immunostaining of mouse blastocyst showed that Setdb1 and Pml signals were scattered in nucleus as a few speckles and microinjected Pmlmyc signals colocalize with Setdb1 signals. This colocalization was observed in mEF and the punctate signals of Setdb1 were observed to be present in every nucleus of mEFs and dividing cells with condensed chromosomes. Arsenic treatment, which induces Pml degradation, also caused Setdb1 signals to disappear. Setdb1 knockdown resulted in disassembly of PML-NBs and immunoprecipitation results demonstrated physical interactions between Setdb1 and Pml. These data suggest that Setdb1 was associated in PML-NB and Setdb1 has important function in maintenance of PML-NB structure.

Heat shock protein 90 (Hsp90) is ATPase-directed molecular chaperon and affects survival of several cells. In our previous study, inhibitory effect of Hsp90 by inducing cell cycle arrest and apoptosis in the pig embryonic and primary cells was reported. However, its role during early bovine embryonic development is not sufficient. In this study, we traced the effects of Hsp90 inhibitor, 17-allylamino-17-demethoxygeldanamycin (17-AAG), on early bovine embryonic development. We also investigated several indicators of developmental potential, including structural integrity, gene expression (apoptosis-related genes), and apoptosis, which are affected by 17-AAG. Bovine embryos were cultured in the CR1-aa medium with or without 17-AAG for 7 days. In result, significant differences in developmental potential were detected between the embryos that were cultured with or without 17-AAG (33.1±9.6 vs 21.7± 8.3%). The structural integrity of the blastocysts was examined by differential staining. Blastocysts from the dbcAMP- treated group had higher numbers of ICM, TE, and total cells than those from the untreated group. Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) showed that the number of containing fragmented DNA at the blastocyst stage increased in the 17-AAG treated group compared with control (11.2 vs 3.9, respectively). Blastocysts that developed in the 17-AAG treated group had low structural integrity and high apoptotic nuclei than those of the untreated control, resulting in decrease the embryonic qualities of preimplantation bovine blastocysts. The m-RNA expression of the pro-apoptotic gene (Bax) increased in 17-AAG treated group, whereas expression of the antiapoptotic gene (Bcl-XL) decreased. In conclusion, Hsp90 also appears to play a direct role in bovine early embryo developmental competence including structural integrity of blastocysts. Also, these results indicate that Hsp90 is closely associated with apoptosis-related genes expression in developing bovine embryos.

The purpose of this study is to understand the transition process of Korean architecture and urbanism from traditional state to modern state, by investigating the development of modern survey and the characteristics of survey drawings during the Great Han Empire (大韓帝國), the early modern Korea. The governmental efforts of the Great Han Empire to introduce a modern survey system named Gwangmu Land survey (光武量田事業) ended in failure. After the Russo-Japanese War (露日戰爭, 1904-1905), the Residency-General (統監府) held the hegemony of Korean Peninsula. It reintroduced a modern survey system for the survey of land and buildings all over the country and enforced the Land and Buildings Certification System (土地家屋證明制度). Since then, the land and buildings survey was propagated rapidly and the modern system for land use was gradually organized. With the progress of modern survey, the survey bureau of Cabinet (內閣) and Department of Royal Household (宮內府) created survey drawings that had some characteristics of colonialism. Takjibu (度支部) produced cadastral maps of major cities, with which the modern land system was developed. In addition, the Royal Property Bureau (帝室財産整理局) produced survey drawings of land and buildings owned by the Royal Household which were finally converted into modern facilities.

쌀가루제품은 밀가루 제품에 비하여 알레르기 유발율이 낮으므로, 영유아용 제품으로 개발될 수 있는 가능성이 크다. 특히, 영유아용 식품은 치아발달이 미숙한 영유아에 적합하도록 침에 의해서 쉽게 녹여 삼킬 수 있어야 하는데, 본 연구에서 개발된 팽화쌀과자는 이러한 특성을 모두 만족하고 있다. 본 연구에서는 쌀가루로부터 쌀압출물을 제조한 후 팽화시켜 다공질이 많은 구조를 갖는 쌀과자를 제조하였다. 팽화쌀과자의 팽화율은 1.8배이었으며, 경도는 대조군으로 연구된 Graduates 및 Little과 유의적 차이를 보이지 않았다. 팽화쌀과자의 수분흡수량은 상대습도가 증가함에 따라 증가하였으며 수분 흡수속도는 상대습도 30-40% 구간에서 0.0024 mg/hr로 가장 낮았다. 본 연구에서 개발된 팽화쌀과자는 기존 쌀가공품이 보이는 딱딱한 물성을 다공성의 구조의 형성을 통하여 개선하였으며, 이는 영유아의 씹힘과 삼킴에 부담을 줄이며 저작운동을 유도하여 치아발달에 도움을 제공할 수 있다. 본 연구의 팽화쌀과자와 같은 팽화쌀제품의 개발을 통하여 기존에 밥, 떡, 주류 중심의 쌀제품에서 탈피하여 쌀가공품의 다양화에 이바지할 수 있다.

A total of 106 two month-old Nanjiang yellow goats (male were 32, female were 34 and castrated were 40) were divided into treatment group and control group to determine the growth performance. The treatment group was reared in grazing mode, experimental group was pastured with supplement feeding. The results showed that the body weight of male in experiment group was heavier by 9.82%(P＜0.05), 11.87%(P＜0.01), 16.91%(P＜0.01) and 23.99%(P＜0.05) than that in control group in 3,4,5 and 6 month-age. The body weight of female in experimental group was heavier by 11.54%(P＜0.05), 13.79%(P＜0.01), 17.47%(P＜0.01) and 21.15%(P＜0.01) than that in control group in 3,4,5 and 6 month-old. The body weight of castrator in experimental group was heavier by 1.58%, 1.99%, 13.08%(P＜0.01) and 15.76%(P＜0.01) than that in control group in 3,4,5 and 6 month-age. The daily increasing weight and body measurements of experimental group is improved more than control group, especially in 6 month-age.

There are replete numbers of reports which have apparently shown that established patterns of methylation are critical for normal mammalian development. Here, we report expression of the DNA methyltransferases (Dnmts) family during mouse early development. Transcription of Dnmt1o occurs in one-cell and morula stage embryos, whereas Dnmt1s transcripts were detectable in all cells and tissues examined during the study. Dnmt3a1 transcript was detected in all cells and Dnmt3a2 transcript was particularly detected in the oocyte and 1-cell stages. Low level Dnmt3b1 transcripts were expressed ubiquitously in oocyte, 1-cell, and preimplantation embryos except 2～4 cell stages. Dnmt3b3 transcripts were only detected in E7.5 embryo and ovary. Furthermore, Dnmt3l transcripts were detectable in all cells and tissues examined. Unlike Dnmt1, both Dnmt3a and Dnmt3b proteins existed in the nucleus of preimplantation embryos till the morula stage. These Results suggest that differences Dnmts expression level exist and genomic DNA methylation patterns may be determined partly through differential expression of Dnmts during early development.

이온 통로 및 이온 농도의 변화는 수정 현상을 포함한 다양한 세포 기능에 중요한 역할을 한다. 그러나 이러한 이온의 변화가 포유동물 배의 발달과정에 어떻게 관여하는지에 대해서는 알려진 바가 적다. 본 연구에서는 생쥐난자가 수정 이후 배 발달 과정을 거치는 동안 나타나는 칼슘과 포타슘 이온의 변화를 전기생리학적 실험 기법과 공초점 현미경을 이용하여 조사하였다. 수정 시에 나타나는 일시적인 세포내 칼슘 농도 변화는 활성 전류(수정 전류)와 함께 동반되었다.