Transient receptor potential melastatin 8 (TRPM8) plays a crucial role in innocuous cool sensation, acute cold pain and cold-induced hyperalgesia during pathologic conditions. To help understand TRPM8-mediated cold perception in the dental pulp and periodontal tissues, we examined the distribution of TRPM8-immunopositive (+) axons in molar and incisor pulp and periodontal tissues using transgenic mice expressing a genetically encoded axonal tracer in TRPM8+ neurons. In the radicular pulp of the molar teeth, a small number of TRPM8+ axons were observed. TRPM8+ axons branched frequently and extensively in the core of coronal pulp, forming a network in the peripheral pulp. Some TRPM8+ axons ascended between odontoblasts and were observed in the dentinal tubule. TRPM8+ axons were linear-shaped in the radicular pulp, whereas many TRPM8+ axons showed portions shaped like beads connected with thin axonal stands at the peripheral pulp. TRPM8 was densely expressed in the bead portions. In the incisor pulp, TRPM8+ axons were occasionally observed in the core of the coronal pulp and rarely observed at the peripheral pulp. TRPM8+ axons were occasionally observed and showed a linear shape rather than a bead-like appearance in the periodontal ligament and lamina propria of the gingival tissue. These findings, showing differential distribution of TRPM8+ axons between radicular and coronal portions of the molar pulp, between incisor and molar pulp, and between dental pulp and periodontal tissues, may reflect differential cold sensitivity in these regions.

본 논문에서는 과도한 계산용량이 필요한 초음속 비행체의 비정상 열응답 해석을 수행하기 위한 준-비정상해석 기법을 소개한다. 준-비정상해석 기법은 연성 연계 기법과 복합 열전달 해석기법을 통합한 방법으로 계산시간 단축시키면서 동시에 정확도를 향상시키기 위해 고안되었다. 또한 준-비정상해석 시, 해석 구간을 분할하기 위한 기준시간을 결정하는 알고리즘을 고안하여 준-비정상해석 기법의 정확도를 향상시키고자 하였다. 본 논문에서는 준-비정상해석 기법을 평가하기 위하여 가상의 비행 시나리오에서 열응답 해석을 수행하였으며, 비정상 해석 결과와 비교 검증을 수행하였다. 무딘 물체의 표면 온도 및 정체점의 온도를 통해 각각의 기법의 차이를 도출하였다. 비정상 해석을 통해 도출한 정체점의 온도와 준-비정상 해석을 통해 도출한 정체점의 온도 차이는 11.4% 이내로 높은 정확도를 확보함과 동시에 28배에 가까운 계산시간을 단축시켜 해석 기법의 효율성과 정확성을 확보하였다.

Recently, measuring instruments for SHM of structures had being developed. In general, the wireless transmission of sensor signals, compared to its wired counterpart, is preferable due to its absence of triboelectric noise and elimination of the requirement for cumbersome cable. Preliminary studies on the continuous vibration measurement of high-rise buildings using MEMS sensors have been carried out. However, the research on the low-rise buildings with relatively small vibration levels is insufficient. Therefore, in this paper, we used the wireless MEMS sensor to compare and analyze the vibration measurements of three low-rise buildings.

왕복동식 압축기에서 피스톤과 커넥팅로드는 중요한 부분이다. 이러한 주요부에 기계적 부하가 과도하게 가해지면 해당 기부 속이 손상될 수 있으며, 교체하기도 쉽지 않고 비용도 많이 든다. 따라서 내구성과 수명에 영향을 미치는 요인을 분석할 필요가 있다. 본 연구의 주요 목적은 피스톤과 커넥팅로드의 최대 응력 집중 위치를 확인하는 것이다. 이를 위해 설계된 공기압축기의 작업 공정의 동적 계산을 기반으로 피스톤 및 커넥팅로드의 응력 분석을 수행하였다. 공기압축기의 피스톤과 커넥팅로드의 3 차원 모델을 따로 설계하고, 이러한 부품들의 유한요소 해석은 수치해석적인 근사해법을 사용하였다. 피스톤은 열 경계 조건 없이 크랭크 샤프트의 각도에 따라 압 력 부하를 받는다. 시뮬레이션 결과는 피스톤과 커넥팅로드의 응력 집중 위치와 그 값을 예측하고 추정할 수 있다. 그 결과 크랭크 각도 135°와 225°에서 피스톤은 190MPa, 커넥팅로드는 123MPa 이상의 최대 등가응력이 나타났으며 이는 인장 항복강도 이하의 값이다. 또한, 커넥팅로드와 피스톤에 계산 된 안전 계수는 1보다 높게 나타났다. 더욱이, 이러한 결과는 왕복동 공기압축기 제작사에 피스톤 및 커넥 팅로드를 설계함에 있어서 최적화를 위한 참고 자료로 활용 될 수 있다.

본 논문은 동역학의 새로운 변분이론인 확장 해밀턴 이론을 열 탄성과 공극 탄성에 적용하여 더욱 일반화하는 것에 그 주 요 목적이 있다. 이를 위해 열 탄성학에 대한 이론 적용이 우선적으로 검토되었고, 열 탄성-공극 탄성의 유사성을 바탕으로 공극 탄성에까지 그 이론이 확장되었으며, 각 경우에 대한 푸리에 변환을 통해 그 적정성을 확인하였다.

본 논문에서는 Co-Rotational plane beam transient analysis EDISON program(CR-보)를 이용한 에어포일 단면형상 변화 에 따른 진동특성 연구를 수행하였다. Co-Rotational 평면 보 해석은 대 회전과 작은 변형률을 갖는 보 해석에 적합하다. 항 공기의 날개를 외팔보로 가정하여, VABS를 통한 단면해석과 Fourier 변환을 통해 각 단면형상 변화에 따른 에어포일의 고 유진동수를 비교하였다. VABS를 사용하여 단면의 형상과 재료의 적층 정보를 고려한 단면에서의 유한요소 해석을 수행하 였다. 에어포일의 재질, spar 유무, 단일 등방성 재료·복합재료, 에어포일 최대두께의 변화에 따라 에어포일의 끝단 진폭과 고유진동수가 변화함을 확인할 수 있었다. 이를 바탕으로 에어포일 고유진동수 변화는 2차 관성모멘트/단면적, 밀도, 영률의 변화에 상당한 영향을 받음을 알 수 있었다.

Acetylcholine receptors (AChR) including muscarinic and nicotinic AChR are widely expressed and mediate a variety of physiological cellular responses in neuronal and non-neuronal cells. Notably, a functional cholinergic system exists in oral epithelial cells, and nicotinic AChR (nAChR) mediates cholinergic anti-inflammatory responses. However, the pathophysiological roles of AChR in periodontitis are unclear. Here, we show that activation of AChR elicits increased cytosolic Ca2+ ([Ca2+]i), transient cytotoxicity, and induction of receptor activator of nuclear factor kappa-B ligand (RANKL) expression. Intracellular Ca2+ mobilization in human gingival fibroblast-1 (hGF-1) cells was measured using the fluorescent Ca2+ indicator, fura-2/AM. Cytotoxicity and induction of gene expression were evaluated by measuring the release of glucose-6-phosphate dehydrogenase and RT-PCR. Activation of AChR in hGF-1 cells by carbachol (Cch) induced [Ca2+]i increase in a dose-dependent manner. Treatment with a high concentration of Cch on hGF-1 cells caused transient cytotoxicity. Notably, treatment of hGF-1 cells with Cch resulted in upregulated RANKL expression. The findings may indicate potential roles of AChR in gingival fibroblast cells in bone remodeling.

Temperature as a major environmental factor affects on organisms on various levels including molecular, physiological, behavioral and ecological levels. Transient receptor potential channels (TRPs) are a cation channel family. Among them, thermo-TRPs are known as a thermosensor. The potential role of thermo-TRPs have been identified in the fruit fly, Drosophila melanogaster, in thermotaxis and in thermal acclimation. With RNA interference (RNAi) technique, the role of thermo-TRPs in the red flour beetle, Tribolium castaneum, was identified by measuring thermal avoidance behavior in a behavioral assay. RNAi of trpA1 reduced high temperature avoidance, 39 and 42 °C. Moreover, the effects of RNAi of thermo-TRPs on the heat-induced knockout and the death after short exposure to high temperature was measured after one minute exposure at 52 °C, either with or without a 42 °C 10-minute thermal acclimation period. Even though it was relatively short time exposure to high temperature, it was enough to induce high temperature thermal acclimation. RNAi of trpA1 made faster knockout at 52 °C. With RNAi of painless, the recovery rates from heat-induced knockout after thermal acclimation. RNAi of pyrexia reduced long-term total survivorship without thermal acclimation.

The Palomar Transient Factory is a project making use of a Schmidt 48 inch telescope located on the Palomar Mountain, which is surveying the sky with dynamical cadences. It was deployed in 2009 and the observed sky region is over 1200 square degrees. We have studied the long-term periodic variabilities of the known galactic cataclysmic variables (CVs). More than 20 of the sources had been found to have long term periodic signals, ranging from several tens of days to several hundreds of days. Some possible scenarios are proposed to explain the results, such as a magnetic field change of the companion star, precession of the accretion disk, triple systems and superoutburst cycles. Some preliminary discussion will be presented in this article.

The aim of the study was to investigate the neural substrates associated with processing anger among young males with alcohol use disorders (AUDs) using functional magnetic resonance imaging (fMRI). Eighteen individuals with AUD and 15 demographically similar non-abusers participated in the study. Participants were scanned on their brain functioning while they viewed an audio-visual film clip that was previously designed specifically to induce anger emotion, followed by anpsychological assessment. Greater brain activities were detected in the left inferior frontal gyrus (IFG) and dorsal anterior cingulate cortex (dACC) among subjects with AUD compared to the controls during the exposure to anger-provoking stimuli. Despite the same level of subjective anger during anger induction, the greater activations both in the IFG and dACC regions may suggestthat individuals with AUD have a greater propensity to undergo cognitive control and self-regulation while experiencing anger.

The cerebellum is known to control balance, equilibrium, and muscle tone. If the cerebellum becomes damaged, the body is unable to retain its balancing functions or involuntary muscle movement. This is why, in stroke patients, there is a high risk of functional disability, as well as a myriad of other disabilities secondary to stroke. Ischemia was induced in SD mice by occluding the common carotid artery for 5 minutes, after which blood was reperfused. Needle electrode electrical stimulation(NEES) was applied to acupuncture points, at 12, 24, and 48 hours post-ischemia on the joksamri. Protein expression was investigated through caspase-3 antibody immuno-reactive cells in the cerebral nerve cells and Western blotting. The results were as follows: The number of caspase-3 reactive cells in the corpus cerebellum 12 and 24 hours post-ischemia was significantly (p<.05) smaller in the NEES group compared to the GI group. caspase-3 expression 12 and 24 hours post-ischemia was significantly(p<.05) smaller in the NEES group compared to the GI group. Based on these results, NEES seems to have a significant effect on Caspase-3 in the cerebellum in an ischemic state at 12 and 24 hours post ischemia, NEES delays the occurrence of early stage apoptosis-inducing Caspase-3, delaying and inhibiting apoptosis. Further systematic studies will have to be conducted in relation to the application of this study’s results on stroke patients.

Bromoviridae과 Cucumovirus속에 속하는 대표 바이러스인 오이모자이크바이러스 (Cucumber mosaic virus: CMV)는 많은 경제적으로 중요한 원예작물 및 관상식물들에 심한 손실을 초래하는 바이러스이다. 다중염기서열 비교는 현재까지 서브그룹1에 속하는 모든 CMV 계통들에서 3'말단부의 보전적 염기서열들이 존재하고 있음을 보여주었다. 이런 관찰에 기초하여, 우리는 CMV RNA3와 상동성을 가지는 162 bp 상보적 DNA를 포함하며 CMV감염에 대하여 식물 유래 RNA간섭 현상을 유도할 수 있는 도치된 반복 구조를 가지는 머리핀RNA (pIR-CMVNCR)를 발현시킬 수 있는 벡터를 제작하였다. 아그로박테리움을 이용하여 IR-CMVNCR의 일시 발현은 CMV 감염을 저해하였으며, 아그로박테리움이 접종된 식물의 상엽에서는 CMV 병징이 발현되지 않았다. 또한 RT-PCR결과는 아그로박테리움이 접종된 식물의 접종엽 및 상엽에서 CMV 서브유전자 4를 포함하는 CMV RNA들이 모두 검출이 되지 않았다. CMV 유래 작은 저해 RNA들의 축적이 관찰되었으며, 이의 결과의 의미는 아그로박테리움에 의해 IRCMVNCR을 일시 발현시킨 야생담배 (Nicotiana benthamiana)의 접종엽에서 RNA 간섭 현상이 유도되어 CMV 감염을 억제시키는 것으로 판명되었다.

This article deals with the transient analysis in bicycle shifting using a discrete chain model. Among the various components of a bicycle, we focused in the power-transmissions on the contact points between the chain element and sprocket. And by imposing kinematic motions on the front and rear derailleurs, we analyzed the shifting mechanism for increasing the rotational speed of rear wheel. In order to build the dynamic analysis model, we first tore down the real bicycle and measured each component's design parameters. Then we made 3-dimensional CAD models for each component related to the power transmission of a bicycle. Using the converted 3-dimensional dynamic model for the simulation program, we performed non-shifting and shifting dynamic analysis. As a result, we investigated the dynamic behaviors of a discrete chain model focused on the interaction between the chain and sprocket wheel.

Ischemia, the leading cause of strokes, is known to be deeply related to synaptic plasticity and apoptosis in tissue damage due to ischemic conditions or trauma. The purpose of this study was to research the effects of NEES(needle electrode electrical stimulation) in brain cells of ischemiainduced rat, more specifically the effects of Poly[ADP-ribose] polymerase(PARP) on the corpus striatum. Ischemia was induced in SD mice by occluding the common carotid artery for 5 minutes, after which blood was re-perfused. NEES was applied to acupuncture points, at 12, 24, and 48 hours post-ischemia on the joksamri, and at 24 hours postischemia on the hapgok. Protein expression was investigated through PARP antibody immuno-reactive cells in the cerebral nerve cells and western blotting. The number of PARP reactive cells in the corpus striatum 24 hours post-ischemia was significantly(p<.05) smaller in the NEES group compared to the global ischemia(GI) group. PARP expression 24 hours post-ischemia was very significantly smaller in the NEES group compared to the GI group. Results show that ischemia increases PARP expression and stimulates necrosis, making it a leading cause of death of nerve cells. NEES can decrease protein expression related to cell death, protecting neurons and preventing neuronal apoptosis.

A 10-year-old intact female Cocker Spaniel weighing 15.3 kg presented with dysbasia. The dog had hyperglycemia and glucosuria at presentation. Neurological examination and magnetic resonance imaging on the following day indicated the onset of cervical disk disease. Four days later, the patient returned due to sudden vomiting and anorexia with severe dehydration. Abnormal laboratory findings included severe hyperglycemia with ketonuria. Plasma progesterone concentration was very high at presentation. She was hospitalized for critical care, and insulin administration with fluid therapy was initiated. Mean blood glucose concentration showed a gradual decline, and hyperglycemia with glucosuria disappeared four days after initiation of insulin therapy. This case demonstrated that high plasma progesterone concentration during diestrus can contribute to development of diabetes mellitus in intact female dogs.

Four different buildings having various wall construction are analyzed for the effect of wall mass on the thermal performance and inside building air and wall temperature transient and also for calculating the energy consumption load. This analytical study was motivated by the experimental work of Burch et al. An analytical solution of one-dimensional, linear, partial differential equations is obtained using the Laplace transform method, Bromwich and modified Bromwich contour method. A simple dynamic model using steady state analysis as simplified methods is developed and results of energy consumption loads are compared with results obtained using the analytical solution. Typical Meteorological Year data are processed to yield hourly average monthly values. This study is conducted using weather data from two different locations in Korea: Daegu having severe weather in summer and winter and Jeju having mild weather almost all year round. There is a significant wall mass effect on the thermal performance of a building in mild weather condition. Buildings of heavyweight construction with insulation show the highest comfort level in mild weather condition. A proportional controller provides the higher comfort level in comparison with buildings using on-off controller. The steady state analysis gives an accurate estimate of energy load for all types of construction. Finally, it appears that both mass and wall insulation are important factors in the thermal performance of buildings, but their relative merits should be decided in each building by a strict analysis of the building layout, weather conditions and site condition.