Various antimicrobial drug screen tests have been used in order to ensure food safety. However, the conventional screen teats, the Swab Test on Premises(STOP, USA), the Calf Antibiotic and Sulfa Teat(CAST, USA) and the European Economic Community 4-plate Test(FPT, EU) are not sufficiently rapid or sensitive enough to detect low levels of sulfa drugs in meat. We developed a new screen test kit for the determination of the antimicrobial residues in meat called the Bacillus megaterium Disk AssayBmDA). A comparison of BmDA with the older screen tests showed BmDA was as good as the older ones with several advantages. The new test kit is faster-it can be read in 4-6 hours instead of 16-18 hours. Moreover, BmDA can discriminate sulfa drugs from other antimicrobial drugs because p-aminobenzoic acid countacts the inhibiting action of sulfa drugs. Minimum detectable levels of sulfa drugs were significantly improved at the level of 0.025-0.1 ppm compared with the level of 1.0 ppm in FPT. A comparison of BmDA with the older screen tests in HPLC confirmed meat samples exeeded the Korean tolerance value of 0.1 ppm showed BmDA was the most sensitive in the microbiological screen tests. As the microbiological screen tests have already known, a person familiar with simple laboratory techniques should have no difficulty in using it to detect antimicrobial residues in meat. This would be a simple, economic method of antimicrobial residues detection which might be succesfully used by many laboratories.

This study was carried out to determine the sex of genomic and embryonic DNA using polymerase chain reaction(PCR). Bovine specific(216bp) and Y chromosome speicific DNA primers(l4lbp) were synthesized and tested for sexing. Bovine embryos used in this study were produced by in vitro fertilization. Few blastomeres for PCR were bisected by nicromanipulator and demi -embryos were cultured in TCM 199 medium containing 0.1% of solcoseryl. The results obtained were as follows; 1. Average optical density of genomic DNA extracted from blood of Hanwoo was 1.79 0.14. 2. 2. The ratio of the demi-embryos developed to blastocyst was 62.1 and 81.9% in morula and blastocyst, respectively. 3. When DNA of 2~4, 5~10 and more than 11 blastomeres was amplified with Y chromosome specific DNA primer by PCR, appreance rate of Y specific DNA band was 16.7, 46.2 and 40.0%, respectively. At least 5 to 10 blastomeres were required to determine the sex of embryos. 4. The rate of demi-embryos developed to blastocyst was 73.3% in TCM 199 medium supplemented with 0.1% solcoceryl. but 55.6% in control.

In order to determine the metallicity of a globuar cluster, M3,by using the spectral indices, a kind of index grid has been establshed by stars in globular clusters, M3, M15, M71 and old open cluster, NGC 188. The indices were measured from the medium resolution spectra of about 2 Å. The summed indices were used to determine metallicity in order to increase signals. It is found that the core depth index is measured more accurately and leads result more accurate than the pseudo-equivalent width index. This method can be further improved by including many more calibration globular clusters of various metallicity to make finer grids. By this method, the metallicity of M3 is determined as [Fe/H] = -1.46±0.15.

순도가 자기적 임계현상에 어떠한 영향을 주는가를 보기 위해 여러 순도의 니켈에서 비저항의 온도의존성을 관찰하였다. 비저항은 Lock-In 증폭기를 사용하여 교류저항측정법으로 측정하였으며 시료를 솔레노이드식으로 감아 저항부분과 인덕터부분의 신호를 함께 관찰할 수 있도록 하였다. 큐리온도는 이들 신호들이 갑작스럽게 변하는 온도로 결정하여UT으며 저항부분과 인덕터부분의 신호에서 결정한 큐리온도값들은 서로 잘 일치함을 볼 수 있었다. 비저항의 온도에 따른 일차미분에서 비열의 임계지수를 결정하였으며 큐리온도와 마찬가지로 임계지수의 순도의존성은 실험오차내에서는 없음이 관찰되었다.

A procedure for the determination of Aflatoxins in food and grains which utilizes reversed phased liquid chromatographic (LC) analysis with postcolumn derivatization by an electrochemical cell and determination with a fluorescence detector has been evaluated. The LC mobile phase was water-acetonitrile-methanol (6+2+2) with 1mM KBr and 1 mM HNO₃ which gave baseline separation for the four Aflatoxins (AfB₁, AfB₂, AfG₁, AfG₂). The electrochemical cell set at 7V, generated bromine and derivatized aflatoxins B₁ and G₁, The derivatives were detected by the fluorescence detector. The aflatoxins in naturally contaminated corn samples were isolated by three different cleanup procedures: the AOAC method I column (CB method), a rapid filtrate column (Romer's column), and an immunoaffinity column. The final extract were quantitated with fluorodensitometric TLC and the LC postcolumn derivatization techniques. The results were quite similar, however the LC technique showed less interferences and could be automated. Samples of corn, raw peanuts, peanut butter and dried dates were also analyzed successfully with this procedure.

This study was carried out to determine and confirm of Dapsone in vegetables and fruits which were illegally used for freshness. We have developed a simple, rapid and precise method that Dapsone can be analyzed in the cabbages, grapes and strawberry by HPLC with photodiode array detector. Experimental subjects were included 15 cases of cabbages, 10 cases of grapes and 10 cases of strawberries purchased in Kangwon, Chungchong province and the Seoul area. The results were obtained that Dapaone in the experimental subject was separated completely within 10 min. Detection limit of Dapsone was 0.5 ng. Aberage recoveries from cabbages, grapes and strawberries were 93.3±0.26%, 92.5±0..37%, 91.4±0..65% respectively. 4 cases of cabbages were detected Dapsone and the amount was below the 0.3 ppm. There was not detected Dapsone in any grape and strawberry samples.

3.3kb 웅성특이 DNA(pEM39 plasmid DNA)가 성 특이 DNA 검색자로 활용되어질 수 있는가를 확인하기 위하여 구조적인 분석을 Southern blotting, DNA sequencing과 computer program 분석을 통하여 실시하였다. 전체 3.3kb에서 유래된 약 1kb 단위의 단편을 이용하여 표지된 짧은 DNA probe들은 Southern blot 분석에서 웅성특이성을 나타내었다. McGraw와 Jeon의 sequence

본 연구는 상대적으로 활하중의 영향이 민감하게 작용하는 농업시설의 합리적 구조설계를 위하여 우리나라 60개 지역의 자료를 사용하여 중요한 설계하중의 결정요인인 단위적설중량, 순간최대풍속 및 최대신적설심의 그 적용에 관하여 검토하였고, 얻어진 결과를 요약하면 다음과 같다. 1. 적설심에 다른 단위적설중량을 분석한 결과 적설심에 따른 차이는 발견할 수 없었고 대체적으로 정규분포를 이루고 있다. 평균기온에 의하여 단위중량을 구분해본 결과 -1℃ 이상에서는 단위중량이 평균 0.91kg/cm/m2, -1℃ 이하에서는 평균 0.58kg/cm/m2로 나타나 기온에 의한 차이가 명확했다. 2. 평균최대풍속과 순간최대풍속과의 관계를 도출하여 회귀식을 유도한 결과, 일반적으로 사용하고 있는 순간최대풍속 관계식과는 약간의 차이를 보였다. 해안지방과 내륙지방에 2개지역으로 구분하여 순간최대풍속 관계식을 유도하였다. 3. 재현기간별 최대적설심과 최대신적설심을 비교한 결과, 눈이 적은 지방에서는 큰 차이가 없으나, 비교적 눈이 많은 지방의 경우 재현기간 8년에서는 24.6cm, 57년에서는 45.6cm로 큰 차이를 보여, 난방 및 적절한 관리를 전제로 설계하면 신적설에 의하여 설계하중을 크게 감소시킬 수 있음을 나타냈다. 4. 기존식과 수정식의 설계풍속(진주지역) 및 최대적설심과 최대신적설심(서산지역)의 적용시, 2연동아치형시설의 부재사용량은 각각의 경우에 대하여 +0.3배, -0.3배이었고, 2연동지붕형 시설의 부재사용량은 각각 +0.3배, -0.1배이었기 때문에 시설의 특성에 맞는 설계자료의 선정이 매우 중요하다.

The depth of sewers in residential complexes was determined to prevent the separated sewers from misconnection between storm sewer and sanitary sewer, and from the submersion of the basement by minimizing the phenomenon of backwater when it rains. In residential complexes, main causes of the submersion were the misconnection of sewers, rising of the backwater level at outfall in sewer system, poor maintenance of sewers, and lacking in their cross section. Minimum depth of sewers should be over 1.2~1.5m. According to the economic analysis, the depth of 1.5m~3.0m was appropriate for minimizing the submersion of basements and for making the disposal of domestic wastewater more easily.