To understand the postharvest characteristics of soybean sprouts, 5-day-old sprouts were harvested, packed in PE film, and stored at 4, 12, and 20~circC for up to 4 days. In addition, the sprout respiration rate was measured after storage at 4, 8, 12, 16, 20, and 24~circC for up to 20h. During the first day of storage at 20~circC , the sprouts maintained temperature-dependent longitudinal growth, especially of hypocotyl length; hypocotyl and root grew 0.8cm and 0.2cm, respectively. The hypocotyl thickness decreased by 11, 13, and 18~% after 4 days of storage at 4, 12, and 20~circC , respectively. No temperature-dependent differences in fresh weight, dry weight, or water content were found, despite decreases of 3~% over the 4 days of storage. A significant postharvest decrease of 50~% in vitamin C content was observed in the sprouts stored at 20~circC for 3days. Based on the CO2 production rate, the soybean sprouts exhibited an increase in respiration in proportion to the storage temperature; sprouts stored at 8, 12, 16, 20 and 24~circC showed approximately 2, 5, 6, 11, and 17 times, respectively, than the respiration rate of sprouts stored at 4~circC . These results indicate the importance of low temperature storage during market circulation for minimizing the postharvest morphological and nutritional degradation of soybean sprouts.

Rehmannia glutinosa shows a high level of resistance to the non-selective herbicide paraquat. To characterize the antioxidant enzyme system of R. glutinosa, we comparatively examined the responses of antioxidant enzymes to UV, wounding and a general elicitor yeast extract in R. glutinosa and soybean. The levels of enzyme activities of the two plant species were drastically different between those per fresh weight (general activity) and per protein (specific activity) bases. The general activities of superoxide dismutase (SOD), peroxidase (POX), catalase (CAT), and glutathione reductase (GR) were lower, but that of ascorbate peroxidase (APX) was higher in R. glutinosa than in soybean. The specific activities of the enzymes, however, were about two- to seven-fold higher in R. glutinosa than in soybean, except that of CAT, which was about 12-fold higher in soybean. The general and specific enzyme activities of R. glutinosa relative to those of soybean showed a consistent increase in responses to the stresses only in SOD. The specific activities of SOD and APX were higher in R. glutinosa in all stress treatments. The results might suggest a relatively higher contribution of SOD and APX to the stress tolerance.

Changes in the level of metabolites in leaves and pods were examined with respect to the seed chemical composition in black soybean. There was no further increase in pod length after 42 days after flowering (DAF). Pod weight, however, persistently increase until 73 DAF, thereafter the weight was slightly lowered. The seed storage protein, however, increased drastically as the increasing rate of pod weight was lessened at 61 DAF. The accumulation of seed storage proteins was occurred conspicuously as the increasing rate of pod weight was slowed down. The chlorophyll content both in leaves and pods was drastically decreased after 50 DAF. The beginning of drastic reduction in chlorophyll content was occurred concomitantly with the reduction of soluble protein content in leaves. The sugar content in leaves showed similar tendency with chlorophyll and soluble protein content. The starch level in leaves, however, showed different changing pattern during seed development. The starch content in leaves was increased persistently until 66 DAF, thereafter the content was decreased drastically to about 55~% of maximal value at 66 DAF. Total phenolics content in leaves and the anthocyanins content in seeds were stable without noticeable increase until 66 DAF. The contents were increased dramatically after 66 DAF showing the synchronized pattern with the decrease in starch level in leaves. The levels of the selected metabolites in leaf and seed suggested that the accumulation of chemical components of black soybean seed is launched actively at 66 DAF. The profile of storage proteins was nearly completed at 61 DAF because there was no large difference in densitometric intensity among protein subunits after 61 DAF. In soybean, chemical maturation of seed begins around 61 to 66 DAF at which most metabolites in vegetative parts are decreased and remobilized into maturing seeds.

Number of nodes and branches are yield component characters whose expression are highly related with many other traits. In this study, a population derived from the cross of ‘Keunolkong’ and ‘Shinpaldalkong’ was evaluated with SSR markers to identify QTLs

This study was carried out to investigate the best explant source and combination of media and growth regulators for the regeneration of multiple shoots in soybean (Glycine max. L. Merr.) cv. ‘Iksannamulkong’. Multiple shoots could well be directly induce

An experiment was conducted to determine N₂-fixation efficiency using 5 soybean genotypes, viz. 'Chiang Mai (CM) 60', 'SJ1', 'Santa Maria', 'KUSL 20004', 'AGS 292' and 40 rhizobium strains from the Soil Microbiology Group, Soil Science Division

The experiment was conducted to determine the effects of enhanced UV-B on growth and differential responses among cultivars in soybean. The soybean cultivars subjected to enhanced UV-B irradiation at daily dose of 11.32 kJ m^-2(UV-B_BE) revealed that the growth was significantly depressed. Plant height, leaf number, leaf area and dry weight were inhibited by UV-B irradiation showing differential responses among cultivars used. Danyeubkong seems to be less sensitive to the enhanced UV-B irradiation, while Keunolkong more sensitive. Reduction of chlorophyll content was also found significantly greater to Keunolkong. Specific leaf weight, an index of leaf thickness, and flavonoid content known as UV-absorbing compounds were significantly increased in Danyeubkong by UV-B, but those in the other cultivars were not significantly affected. The results indicated that there are cultivar differences in the growth and physiological responses to the enhanced UV-B irradiation and specific leaf weight and UV-absorbing compounds in the leaves were highly related to the sensitivity of soybean by UV-B irradiation.

대두(Glycine max L.) 종자로부터 분리된 렉틴을 토끼에 면역화시켜 렉틴항혈청을 얻었다. 이를 이용한 면역화학적 방법을 사용하여 대두렉팅의 면역화학적 특성과 6개 대두 품종의 종자에서 렉틴의 존재를 조사하였다. Crossed immunoeiectrophoresis한 결과 종자는 4종의 침강선을 보임으로서 렉틴에 4종의 항원성분이 존재함을 보여 주었다. 1％ agarose gel을 이용하여 Immunodouble diffusion test한 결과 6개 대두 공시품종 모두 렉틴항체에 대하여 침강선을 나타내었고 푸종간 침강선은 모두 유사한 양상을 보였다. ELISA 방법에 의하여 6개 대두 품종간의 렉틴함량을 조사, 비교한 결과 렉틴함량은 장엽콩>광교>황금콩>백운콩>장백콩=팔달콩 순으로 나타났다. 공시품종의 조자로부터 분리한 렉틴은 모두 토끼 적혈구는 응집시켰으나 Fusarium sp., Alternaria sp., Cuvalaria sp. 등의 곰팡이 포자는 응집시키지 못하였다.

본 실험은 대두(Glycine max L.) 방사품종의 종자단백질에서 렉틴을 분리하여 그 특성을 조사하기 위하여 수행되었다. 렉틴의 분리방법은 (NH4 )2 SO4 의 50~80% 포화침전단백질을 Carboxymethyl cellulose column과 Sephadex G 100 column을 거쳐서 분리, 정제되었다. 순도의 검정은 2~30% polyacrylamide porosity gradient gel을 사용한 전기영동법으로 단일 band를 확인하였고 또한 분자량은 11% sodium dodecyl sulfate polyacrylamide gel에 의하여 134,000 dalton임을 추정하였으며 45,000 dalton의 3개의 subunits로 구성되어 있음을 알았다. Schiff reagent에 의하여 분홍색 반응을 일으켰으므로 이것은 당단백질임을 알 수 있었다. 본 당단백질은 토끼와 사람의 적혈구 모두에서 적혈구응집 반응이 일어났으며 사람의 혈액형중에서 적형구응집 정도는 A>B>O>AB형의 순서로 응집이 잘 일어났다. 당류에 의한 적혈구응집저해는 N -acetyl-D-galactoseamine 과 D-galactose에 의하여 일어났다.

추대두형품종을 조파 또는 적파 재배하여 개체당 생육량을 증대시키면서 건실한 생육을 유동하기 위하여 생장조절제를 처리하였을 때 이들이 콩의 생육 및 수량형질에 영향하는 바를 조사한 결과를 요약하면 다음과 같다. 1. 조파할 경우 적파에 비하여 주경절수, 분기수, 협수, 100립량 등의 형질들이 증가되는 경향을 보였으며 10a당 수량도 8% 증가했다. 2. TIBA 또는 ABA 처리는 경장단축, 경직경증대 및 도복경감 등 건실한 생육을 유도하는데 효과적이었으며 결협률 주당입수를 증대시켜 종실수량이 6% 이상 증가하였다. 특히 TIBA 처리에 의해서는 12%의 수량이 증대되었다. 3. 생장조절제 처리횟수에 있어서는 6엽기에서부터 5일 간격으로 2∼3회 처리하는 것이 건실한 생육을 유도하여 수량이 증대되었으며 3회 처리구에서는 도복경감, 결협률 및 천립량 증대효과가 크게 나타나 16%의 수량이 증대되었다. 4. 본 실험에 사용한 생장조절제 중에서느 TIBA의 효과가 가장 좋아서 조파구나 적파구에서 모두 TIBA 3회 처리구에서 20% 이상의 수량이 증대되었다.

하대두형 및 추대두형 콩품종에 대하여 파종기를 달리 했을 때 생육상의 차를 구명하고, 또한 건물축적양상 비교하기 위하여 1987년 천안시 안서동에 위치한 단국대 농대 실습포장에서 시험을 수행하였다. 극조반(4월 15일파종)나 그후 파종(5월 15일 및 6월 15일)에서 하ㆍ추대두형품종간 출현율의 차가 관찰되지 않아 포장에서 저온발아성의 차를 검정한다는 것은 어렵다고 판단되었고, 또한 하ㆍ추대두형간 개화일수단축정도의 차도 없었으나, 하대두형인 DN82029-3이나 ES18085-1의 조ㆍ만파기 생육일수의 차가 추대두형인 장엽콩이나 팔달콩에 비하여 크지 않아, 생육후기 고온에 의한 결실일수가 촉진되어 만파에서 추대두형에 비하여 몇가지 수량구성요소의 감소정도가 큰 것으로 나타났다. 하대두형은 추대두형에 비하여 만파에서 도장정도가 심함 것으로 관찰되었다. 하대두형인 DN82029-3이이나 추대두형인 단엽콩 및 장엽콩은 개화시 이후에도 경엽신장이 큰 폭으로 증가된 바, 개화시 이후 3품종 모두 경장이 17∼32cm가 신장되었으며, 엽면적지수도 개화시 이후 0.83∼1.53의 증가를 보여 엽의 신장이 큰 폭임을 나타내 주었다. 하대두형인 DN82029-3은 단엽콩이나 장엽콩에 비하여 49일이나 빨리 최고건물중을 나타냈으나, 절대량은 추대두형의 최고건물중의 약 50% 수준이어서 5월 15일 파종시 일장둔감형인 하대두형은 충분한 영양생장을 이루지 못한 가운데 생식생장기 간으로 접어들어 건물축적량이 크게 낮아지게 되고, 이것이 낮은 수량의 원인으로 보인다.