밀은 세계 3대 주요 작물이지만 우리나라는 대부분 수입에 의존하고 있으며, 자급률이 1%도 도달하지 못한 실정이다. 국내 밀 품종은 40여 종 이상으로 지속적으로 개발되고 있으며, 품종마다 용도와 농업적 특성이 달라 목표 형질에 맞는 적절한 품종을 선택하여 재배하는 것이 중요하다. 품종을 정확하고 빠르게 판별하기 위해선 분자 마커 기술이 필요하다. 분자 마커는 생육 환경과 시기에 영향을 받지 않고 다양한 품종을 신속하고 정확하게 구분할 수 있어 유전적 변이성, 농업 형질 등을 분석하기에 유용하다. 본 연구는 기존에 보고된 국내 밀 32품종에 대한 SCAR (Sequence Characterized Amplified Region) 판별 마커를 재검정하여 재현성이 높은 마커를 선별하였으며, 기존에 검정되지 않은 국내 밀 9품종에 추가 적용하여 비교분석하였다. 15개의 마커 세트 중 6개의 마커가 재현성과 정확도가 높은 것으로 확인되었고, 최종적으로 국내 41품종 중 4, 7, 8, 10, 11, 12번 마커를 이용하여 다홍, 금강, 밀성, 조은, 수강, 한백, 조광, 영광을 판별할 수 있었다. 또한, 4번 마커를 통한 증폭산물의 염기서열 분석을 통해 SNP (Single Nucleotide Polymorphism)를 발굴하여 ‘올밀’에 대한 새로운 품종 판별 마커인 SdHRM1과 SdHRM2를 개발하여 HRM (High Resolution Melts) 분석을 시행하여 육안으로 판단하기 어려운 SNP를 정확하게 판별할 수 있었다. 품종간의 SNP를 활용한 품종 마커 기술은 다양한 농업형질에 적용할 수 있으며, 분자 육종 프로그램에 실질적인 도움이 되는데 큰 기여를 할 것으로 사료된다.

Recently, extreme terrorist attacks have frequently occurred around the world and are threatening the international community. It is no longer a safe zone for terrorism in our country. Therefore, domestic nuclear facilities as the highest level of national security facilities have established a physical protection system to protect facilities and lives against terrorist attacks. In addition, security search and access control are conducted for controlled items and unauthorized person. However, with the development of science and technology, disguised weapons or homemade explosives used in terrorism are becoming very sophisticated. Therefore, nuclear facilities need to strengthen security search of weapons or homemade explosives. Since these disguised weapons or homemade explosives are difficult to find only through security search, it is also necessary to actively identify unspecified people who possess disguised weapons or do abnormal behavior. For this reason, the “Abnormal Behavior Detection Method”, which is very effective in preemptive response to potential terrorist risks, has been introduced and operated in aviation security field. Korea Institute of Nuclear Nonproliferation and Control (KINAC) has established a “Practice Environment for Identifying Disguised Weapons” in 2020 for trainees to recognize the dangers of controlled items and to use for physical protection education. This Practice environment has not only the basic explanation of the controlled items of nuclear facilities, but also various actual disguised weapons were displayed. It also introduces actual terrorist incidents using homemade explosives such as attempted bombing of a cargo plane bound for Chicago and the Boston Marathon bombing. And then a model of the disguised explosives actually used is displayed and used for education. In addition, in 2022, the “Abnormal behavior detection method” education module was developed and used for physical protection education. In this module, the outline and introduction of the “Abnormal Behavior Detection Method” and “Behavior Detection Officer (BDOs)” are explained. In this way, the access control and security search system of nuclear facilities require the overall monitoring system, not only for dangerous goods but also for identification of persons possess and carrying them. This study describes the development of the Curriculum for “Disguised Weapon Identification” and “Abnormal Behavior Detection Method” to enhance the effectiveness of physical protection education.

The IAEA states that in the event of sabotage, nuclear material and equipment in quantities that can cause high radiological consequences (HRC), as well as the minimum systems and devices necessary to prevent HRC, must be located within one or more vital areas. Accordingly, in Article 2 of the ACT ON PHYSICAL PROTECTION AND RADIOLOGICAL EMERGENCY, the definition of the vital area is specified, and a nuclear facility operator submits a draft to the Nuclear Safety and Security Commission to establish vital areas and must obtain approval from Nuclear Safety and Security Commission. Since the spent fuel pool and new fuel storage area are areas where nuclear material is used and stored, they can be candidates for vital areas as direct targets of sabotage. The spent fuel pool is a wet spent fuel storage facility currently operated by most power plants in Korea to cool and store spent nuclear fuel. Considering the HRC against sabotage, it is necessary to review whether sepnt fuel pool needs to establish a vital area. In addition, depending on the status of plant operation during the spent fuel management cycle, the operation status of safety systems to mitigate accidents and power system change, so vital areas in fuel handling building (including spent fuel pool) also need to be adjusted flexibly. This study compares the results of the review on whether the essential consideration factors are reflected in the identification of essential safety systems and devices to minimize HRC caused by sabotage in the spent fuel storage system with the procedure for identifying the vital area in nuclear power plants. It was reviewed from the following viewpoints: Necessity to identify necessary devices to minimize the radiation effects against sabotage on the spent fuel pool, Review of necessary elements when identifying vital areas to minimize the radiation effects of spent fuel pool against sabotage, Necessity to adjust vital areas according to the spent fuel management cycle. The main assumptions used in the analysis of the vital area of the power plant need to be equally reflected when identifying vital areas in spent fuel pool. And, the results of this study are for the purpose of minimizing the radiological consequences against sabotage on the spent fuel storage system including the spent fuel pool and used to establish regulatory standards in the spent fuel storage stage.

The buffer is a critical barrier component in an engineered barrier system, and its purpose is to prevent potential radionuclides from leaking out from a damaged canister by filling the void in the repository. No experimental parameters exist that can describe the buffer expansion phenomenon when Kyeongju bentonite, which is a buffer candidate material available in Korea, is exposed to groundwater. As conventional experiments to determine these parameters are time consuming and complicated, simple swelling pressure tests, numerical modeling, and machine learning are used in this study to obtain the parameters required to establish a numerical model that can simulate swelling. Swelling tests conducted using Kyeongju bentonite are emulated using the COMSOL Multiphysics numerical analysis tool. Relationships between the swelling phenomenon and mechanical parameters are determined via an artificial neural network. Subsequently, by inputting the swelling tests results into the network, the values for the mechanical parameters of Kyeongju bentonite are obtained. Sensitivity analysis is performed to identify the influential parameters. Results of the numerical analysis based on the identified mechanical parameters are consistent with the experimental values.

Subgroups of sperm which share similar motility features documented in mammals indicate between-subject variations that might be related to fertilizing potential of the respective ejaculates. The objectives of this study were to define subpopulations of motile sperm in Gyr falcon semen using kinematic parameters driven by Computer Assisted Semen Analysis (CASA) and to investigate the subjectrelated variations in these subpopulations. A total of 24 fresh ejaculates from 6 falcons were used to assign each of the 20473 sperms into 3 subpopulations by a multivariate cluster analysis. The proportion of sperms in different sub-populations were compared among subjects by a generalized linear model and repeatability of sperm frequency in different subpopulations was investigated by corelation analysis. The resulting 3 categories of sperm indicated significant differences in all kinematic parameters (p < 0.05). Subpopulation 1 (15.91%) contained sperms with the highest velocity and progressiveness of movement trajectory while subpopulation 3 (6.4%) included the least progressively motile sperms. Proportion of rapid and medium progressive sperm were consistently higher in the ejaculate of three falcons compared to the two other birds which also had the highest proportion of slow non-progressive sperms (p < 0.05). Respective proportion of sperms in each subpopulations indicated significant repeatability over multiple measurements (p < 0.05). In conclusion, subpopulations of motile sperm in Gyr falcon can be identified using kinematic parameters generated by CASA. Individual differences in the proportion of these subpopulations might have potential application for identifying the males with higher fertilizing capacity.

국제해사기구에서는 해상에서의 선박의 충돌방지와 그로 인한 해양환경의 보호를 목적으로 1993년 11월 선박조종성능에 대 한 잠정지침을 채택한 이후, 축적된 데이터를 바탕으로 2002년 12월 선박조종성능에 대한 확정된 지침을 채택하였다. 하지만 채택된 지침은 만재상태, 등흘수 및 선박의 최대 출력(MCR)의 85 %에 해당되는 선속의 최소 90 % 이상에서의 지침으로, 동 지침은 항해사에게 필요한 실 항해조건에서의 조종성능 정보를 제공하는 데 한계가 있다. 따라서 본 연구에서는 항해사, 선장 및 선박조종에 대한 식견을 갖추고 있는 전문가를 대상으로 빈도분석과 AHP 분석기법을 실시하여 현 지침에 대한 활용도 및 실제 선박조종에 필요한 정보가 무 엇인지를 식별하였다. 연구결과 선박을 운항하는 항해사에게 필요한 조종성능 정보는 5~10°의 소각도에서의 선회권 정보, 항해속력(Sea speed)이 아닌 조종속력(Maneuvering speed)에서의 z-test 정보라는 것을 확인하였고, 속도제어 관련하여서는 항해속력 및 만재상태에서의 정지성능에 대한 정보보다 감속타력, 가속타력 등에 대한 정보가 더 필요하다는 것을 확인하였다. 도출된 결과는 선박을 조종하는 항 해사에게 필요한 선박조종성능기준 지침마련에 대한 기초자료로 활용도가 높을 것으로 사료된다.