This study was conducted to investigate the effects of co-culture for the development rate to morula /blastocyst stages of early porcine embryos, derived from oocytes matured and fertilized in vitro, with porcine endometrial cell monolayers(PEM) in the two different media, respectively. The rates of embryos developed to 2-, 4-, 8~16-cell and morula /blastocyst stage were 49.6, 40.5, 28.2 and 15.3% in Ham's F-10 with PEM, and 55.3, 45.9, 32.7, and 17.6% in TCM-HEPES with PEM, respectively. The above development rates to morula /blastocyst stages were significantly higher than those of the embryos cultured in the Ham's F-10 and TGM-HEPES without PEM(P<0.05). The in vitro development rates to the morula /blastocyst stage of 1-cell embryos cultured in Ham's F-10 and TCM-HEPES without PEM were 0~1.2%. Especially, most of embryos were observed to arrest the development beyond 4-cell stages. As shown in the above results, the co-culture of in vitro produced porcine embryos with PEM in the two different media enhanced the development of fertilized eggs to morula /blastocyst stages in vitro. However, we didn't find out any differences for the in vitro development to morula /blastocyst stages between Ham's F-10 and TcM-HEPES media.

The present study was carried out to investigate the effects of co-culture cells and growth factors on in vitro culture of Korean native cattle(KNC) embryos fertilized in vitro. Two-eight cell embryos were cultured in vitro using 4 types of co-culture cells and 3 growth factors singly or in combination. The results were as follows, In the co-culture of 2~8 cell embryos with bovine oviductal epithelial cell(BOEC), granulosa cell(BGC), uterine epithelial cell(BUEC) and mouse embryonic fibroblast (MEF) monolayers, the developing rate to blastocysts was significantly(P<0.05) higher with BUEC(32.1%) than with MEF(15.3%), BGC(13.2%) and non co-culture control(11.6%). When the morula co-cultured with BOEC for 5 days following in vitro fertilization were co-cultured with BOEC continuously or with BUEC, respectively, the developing rate to blastocysts was higher with BUEC(73.9%) than with BOEC(56.0%). To examine the effects of growth factors on in vitro development of 2~8 cell embryos, epidermal growth factor(EGF), transforming growth factor-l(TGF-l) and insulin-like growth factor-1(IGF-1) were added singly or in combination to TCM 199 maturation medium with respective concentration. In a addition of each 10, 30 and SOng /rnl EGF, the developing rate to blastocysts was the highest in lOng /ml EGF(25.3%). In addition of each 1, 2 and Sng /mi TGF-1, the developing rate to blastocysts was the highest in lng /ml TGF-1(28.8%). In addition of each 50, 100ng/ml JGF-l, the developing rate to blastocysts was higher in 100ng/ml IGF-l(16.5%) than in SOng/mi IGF-1(12.9%). When lOng /ml EGF and lng /ml TGF-l was added singly or in combination, the developing rate to blastocysts was similar in groups added singly or in combination with EGF and TGF-l (23.l~24.6%), although higher than in control(16.7%). In the co-culture of 2~8 cell embryos Wth BOEC + each 10, 30 and 5Ong /rnl EGF, the developing rate to blastocysts was significantly(p<0.05) higher in BOEC + long /ml EGF(32.3%) than in BOEC + 3Ong /ml EGF(18.9%) and BOEC + song /ml EGF(9.7%). In the co-culture of 2~8 cell embryos with BOEC + each 1, 2, Sng /ml TGF-l the developing rate to blastocysts was higher in BOEC + Sng/rnl TGF-l(28.2%) than in BOEC + lng /ml TGF-l(21.7%) and BOEC + 2ng/ml TGF-l(21.4%). In summary, higher developing rate to blastocysts were obtained with co-culture of BUEC for co-culture system, with addition of lOng /ml EGF or lng /ml TGF-l for growth factor culture system, and with co-culture of BOEC + lOng /ml EGF or BOEC + Sng /ml TGF-l for co-culture + growth factor culture system.

This study was conducted to improve the in vitro maturation(JVM), in vitro fertilization (IVF) and in vitro developmental capacity of oocytes derived from slaughtered Korean native cattle. The recoverd oocytes, obtained from a local slaughter house, were used completely surrounded by at least 3 layers of cumulus cells in combination with a homogeneous cytoplasmic pigmentation. In vitro maturation was induced in TCM-199 or Ham's F-10 supplemented with LH(1O g/rnl), FSH(35 g/ml), estradiol-17(1 g/ml) at 39 under 5% in air for 24 hours. Sperm from caudal epididyrnis and previously matured cumulus-oocytes complexes were cultured for 24 hours in 100 l droplets of fertilization media under paraffin oil. The zygotes were cultured with media(TCM-199 with bovine oviductal epithelial cells or CRlaa) for 7 to 10 days. The cleavage rate of IVM-IVF oocytes was significantly (P<0.05) higher following maturation using Ham's F-10 (59.9%) than TCM-199 (51.6%). Development to the blastocysts among cleaved embryos was not signficantly different between maturation media: Ham's F-10 (16.0%) and TCM-199(11.9%). However, the hatching rate was affected significantly (P<0.05) on rnaturation media as 62.9% in Ham's F-10, compared with 41.2% in TCM-199. The cleavage rate of IVM-IVF oocytes was significantly (P<0.05) higher following IVF using m-TALP medium (80.1%) than BO medium (51.6%). The percentage of in vitro developed blastocysts among cleaved embryos was not signficantly different between fertimization media: BO (11.7%) and m-TALP (17.6%). The cleavage and the developmental rate to the blastocysts after IVF in m-TALP or condition medium(CM) with or without oviduct epithelial cell monolayer(OECM) was similar(80.1% and 17.6% in m-TALP, 83.8% and 19.4% in M-TALP with OECM. 82.9% and 18.9% in CM, 87.6% and 16.0% in CM with OECM, respectively). The percentage of in vitro developed blastocysts among cleaved embryos was significantly (P<0.05) higher in TCM-199 medium co-cul tured with bovine oviduatal epithelial cell monolayers(35.2%) than CRlaa medium(1.9%). These results stggest that the most transferable IVF embryos could be produced from Ham's F-10, m-TALP and TCM-199 medium with bovine oviductal epithelial cell monolayers for IVM, IVF and IVC, respectively.

The object of this study was to evaluate the effect of uterine epithelial cells on development of Korean native cattle(KNC) oocytes fertilized in vitro. Qocytes were collected from ovaries of slaughtered Korean Native Cows and matured in TCM199 with granulosa cells supplemented with 10% FBS, 5g/ml FSH, 10 JU/ml hCG, and 1g/ml estradiol-17 for 24 hrs. For co-culture of in vitro development of fertilized ova, oviductal epithelial cells (ll0˚cells /ml) obtained from slaughtered cow and uterine epithelial cells(110˚cells /ml) flushed from the superovulated holstein on Day 7 were incubated in 39, 5% , 95% air. Frozen-thawed KNC sperm was capacitated with BO(Brackett & Oliphant, 1975) medium supplemented with 10mM, 5mM-caffein. Matured oocytes were inseminated for 20 hrs. And then fertilized oocytes were washed with culture medium and transferred to oviductal epithelial cells for in vitro development and three days later a portion of embryos were transferred to uterine epithelial cells. Stastical methods of developmental rates on KNC-IVF oocytes was ANOVA-test. Developmental rates of KNC-IVF oocytes was significant higher(P<0.01) when co-cul-tured with uterine epithelial cells(25.2%) than oviductal epithelial cells. Blatocyst cul-tured for 7 to 9 days were frozen by automatic freezer with 1.4M glycerol-PBS. Survival rates of blastocyst was 40.0%. Fourteen frozen-thawed blastocysts were transferred to five holstein heifers on day 7 after natural estrus. Three recipients were observed twin and one recipient was single by ultra-sound systems on days 45 after embryo transfer.

간격침적식 수경재배의 실용화 가능성을 검사하고자 10분간격 간격침적식, 15분간격 간격침적식, NFT식 및 분무경system을 이용하여 토마토에 대한 생육비교 실험을 수행하였다. 1. 초장은 NFT식에서 가장 양호하였으나 주근장은 간헐침지식에서 길었으며 수경과 엽수는 처리간에 차이가 없었다. 2. 생체중과 건물중은 생육초기에는 NFT에서 무거웠으나 생육이 진전될수록 간헐침지식에서 더 무거워졌다. 3. 처리 후 43일의 개화수는 분무경 18.5, 15분간격 간헐침지식 17.8, 10분간격 간헐침지식 16.8, NFT 15.1개 순이었으며 과중은 분무경에서 149.1g으로 가장 무거웠다. 4. 식물체당 평균수량은 분무경에서 1929.1g으로 가장 많았고 10분간격 간헐침지식에서 1475.2g으로 다음이었으며, NFT식 및 15분간격 간헐침지식에서 각각 1276.2, 1084.8g으로 가장 적었다. 5. 식물체당 평균수호과수는 분무경 11.0, NFT식 9.7, 15분간격 간헐침지식 8.3, 10분간격 간헐침지식 7.7개 순이었으며 평균과중은 분무경 192.9, 10분간격 간헐침지식 172.4, NFT식 134.0, 15분간격 간헐침지식 126.0 순이었다. 6. 평균과장은 분무경과 10분간격 간헐침지식에서 NFT식과 15분간격 간헐침지식보다 길었으며 평균과폭은 NFT식, 분무경 및 10분간격 간헐침지식에서 비슷한 정도로 15분간격 간헐침지식보다 켰다.

외국어를 습득함에 있어 언어적인 장애만을 생각하기 쉬우나 실제로는 크고 작은 문화적인 장벽(cultural barriers)이 차지하는 비중 또한 큰 것이다. 본 연구는 문화 지도 자료의 개발이 영어 교실에서 문화 교육의 효율을 높이고, 문화 교육이 영어를 통한 의사소통 능력을 극대화할 것이라는 이해에 근거하였다. 이에 학교 현장의 영어 수업에 직접 활용할 수 있는 문화 지도 자료를 설계 ⋅개발하여 현장에서 활용할 수 있도록 하는데 목적을 두었다. 본 연구는 체제적 교수 설계 과정을 바탕으로 요구 조사 및 분석, 기초 자료 분석, 자료의 수집, 자료 개발 및 평가를 통한 수정⋅보완의 단계를 거쳤다. 요구 분석과 수집된 생생한 사진 및 비디오 자료를 바탕으로 저작도구를 이용하여 자기 주도적 학습과 교실에서의 수업 지도 자료로서의 기능을 지닌 교수⋅학습 자료를 제작하였다. 제작된 지도 자료의 전반적인 보완 및 개선을 위하여 프로그램의 적합성에 대한 평가를 실시 하였다. 영어 교사 평가 결과 제작된 프로그램을 교사의 수업 자료제작에 대한 부담 없이 효율적으로 사용할 수 있다는 점 등이 장점으로 드러났으며 학생의 흥미와 동기부여에 도움이 됨을 알 수 있었다.

This study developed diagnostic system to understand the level of welfare cultural service and infrastructure in rural areas. The applicability was reviewed through the Delphi survey and the sample survey of 60 villages. The diagnostic indicators consist of three areas: the demand area, supply area, and delivery area. The demand area consists of 8 indicators, 25 indicators for welfare services and infrastructure (healthcare, social welfare) and 32 indicators for cultural services and infrastructure (culture, education, leisure sports). The service delivery area was divided into service supply area access and traffic accessibility (public transport use status and rural transport model status) by each indicator. A diagnostic system was applied to 60 villages. Services and infrastructure for rural welfare and culture were supplied more in the Si area than Gun area. The delivery area was easier to access the Gun area than Si area. In the case of traffic access, public transportation was more frequently used in the Si area than Gun area, and the rural transportation model was found to have a relatively large amount in the Gun area compared to Si area. The diagnosis system about services and infrastructure for rural welfare and culture will provide information necessary for establishment and decision making of regional development policy taking into account characteristics of rural areas in the future.