본 연구에서는 약콩(Glycine soja Siebold et Zucc.) 분획 추출물의 미백효능을 관찰하기 위해 B16F10 멜라노마 세포에서 TRP-1 (tyrosinase related protein-1), TRP-2 (tyrosinase related protein-2), 티로 시나제 발현을 평가하였다. 그 결과 약콩 분획 추출물 0.125, 0.25, 0.5, 2.0 mg/mL 농도에서 82% 이상의 높 은 세포생존율을 나타내었다. α-melanocyte stimulating hormone (α-MSH)을 처리한 B16F10 멜라노마 세포에 약콩의 EtOAc 분획 추출물을 처리한 결과 티로시나제 발현이 감소되었으며 TRP-1, TRP-2 단백질 발현이 감소하였다. 이러한 결과는 약콩 분획 추출물이 멜라닌생합성과 관련되는 단백질의 발현을 감소시켜 피 부 미백효능을 나타내는 것으로 기대할 수 있다.

This study was conducted to analyze the polyphenol contents and antioxidant activities of small-black-bean- Chungkukjang-added black food (SBCB) and soybean Chungkukjang (SC) extracts for the development of functional materials. The yields of the hot-water-and-ethanol SC extracts were higher than those of the hot-water-only SC extracts, which were higher than those of the ethanol-only SC extracts. The total phenol contents of the hot-water-and-ethanol SBCB extracts were higher than those of the other extracts. The EDA values of the hot-water-and-ethanol SBCB extracts were higher in the 0.625~5.0 mL/mL extract concentration than those of the other extracts, and those of the SC extracts were high in the 10 mg/mL extract concentration. The SOD-like activities of the hot-water- and ethanol-only SC extracts were higher than those of the other extracts. The nitrite scavenging ability of the ethanol-only SC extracts was higher at pH 1.2 than that of the hot-water-only SC extracts. The xanthine oxidase inhibitory activities of the hot-water- and ethanol-only SBCB extracts were higher in the 0.625 mL/mL extract concentration than those of the other extracts, and increased along with the extract concentration. The inhibitory activities of tyrosinase also increased along with the extract concentration, and the reducing power increased along with the extract concentration and was high in the hot-water-only SBCB and SC extracts.